Raf Raf oncogene [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Rafprovided by FlyBase
Official Full Name: Raf oncogeneprovided by FlyBase
Primary source: FLYBASE:FBgn0003079
Locus tag: Dmel_CG2845
See related: AllianceGenome:FB:FBgn0003079
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 11-29; C110; CG2845; D-raf; D-Raf; D-RAF; D-raf1; Dmel\CG2845; draf; dRaf; Draf; DRaf; Draf-1; Draf1; DRaf1; EG:BACH48C10.3; l(1)2Fe; l(1)G0475; l(1)ph; l(1)phl; l(1)pole hole; l(1)polehole; l(1)polehole/draf; l(1)raf; lincRNA.937; ph; ph1; phl; Phl; raf; RAF; raf-1; Raf/phl; raf1; Raf1; rafl
Summary: Enables MAP kinase kinase kinase activity and kinase binding activity. Involved in several processes, including gamete generation; regionalization; and transmembrane receptor protein tyrosine kinase signaling pathway. Predicted to be located in cytoplasm and plasma membrane. Is expressed in several structures, including anterior endoderm; anterior endoderm anlage; embryonic/larval midgut; extended germ band embryo; and oocyte. Used to study cancer and cardiomyopathy. Human ortholog(s) of this gene implicated in several diseases, including Noonan syndrome (multiple); Noonan syndrome with multiple lentigines (multiple); carcinoma (multiple); cardiofaciocutaneous syndrome (multiple); and central nervous system benign neoplasm (multiple). Orthologous to several human genes including ARAF (A-Raf proto-oncogene, serine/threonine kinase). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

Location:: 2F5-3A1; 1-0.9 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	X	NC_004354.4 (2295466..2343870)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	X	NC_004354.3 (2189499..2237903)

Chromosome X - NC_004354.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Co-dependent regulation of p-BRAF and potassium channel KCNMA1 levels drives glioma progression.
Title: Co-dependent regulation of p-BRAF and potassium channel KCNMA1 levels drives glioma progression.
Optical control of ERK and AKT signaling promotes axon regeneration and functional recovery of PNS and CNS in Drosophila.
Title: Optical control of ERK and AKT signaling promotes axon regeneration and functional recovery of PNS and CNS in Drosophila.
MEK inhibitor cobimetinib rescues a dRaf mutant lethal phenotype in Drosophila melanogaster.
Title: MEK inhibitor cobimetinib rescues a dRaf mutant lethal phenotype in Drosophila melanogaster.
Raf-activated intestinal stem cells and their progenies are very sensitive to apoptosis
Title: Wildtype adult stem cells, unlike tumor cells, are resistant to cellular damages in Drosophila.
Yorkie (Yki), a transcriptional coactivator in the Hippo pathway that regulates organ size, is required for Raf-induced cardiac hypertrophy in flies
Title: Cardiac hypertrophy induced by active Raf depends on Yorkie-mediated transcription.
move from the oocyte to the follicle cells in process that does not require endocytosis
Title: Germline and somatic vitelline proteins colocalize in aggregates in the follicular epithelium of Drosophila ovaries.
These results suggest that ERK is necessary, but not sufficient, for Raf-mediated cardiac hypertrophy.
Title: Raf-mediated cardiac hypertrophy in adult Drosophila.
RNAi-mediated inhibition of MAPK-signaling by knockdown of Ras85D, phl/D-Raf or Dsor1/MEK affects larval development, adult life span and hemolymph phosphate, suggesting that some in vivo effects involve activation of this signaling pathway by phosphate.
Title: Genetic determinants of phosphate response in Drosophila.
RAF catalytic function is regulated in response to a specific mode of dimerization of its kinase domain, which we term the side-to-side dimer
Title: A dimerization-dependent mechanism drives RAF catalytic activation.
tyrosine 510 regulates Draf activation by affecting the autoinhibitory interaction between the N- and C-terminal fragments of the protein
Title: Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables MAP kinase kinase kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in ERK1 and ERK2 cascade	IDA Inferred from Direct Assay more info	PubMed
involved_in MAPK cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in R7 cell fate commitment	IGI Inferred from Genetic Interaction more info	PubMed
involved_in R7 cell fate commitment	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Ras protein signal transduction	HGI more info	PubMed
involved_in border follicle cell migration	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in compound eye photoreceptor development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dorsal appendage formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in dorsal/ventral axis specification, ovarian follicular epithelium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in epidermal growth factor receptor signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in epidermal growth factor receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in epithelial cell migration, open tracheal system	IGI Inferred from Genetic Interaction more info	PubMed
involved_in fibroblast growth factor receptor signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in fibroblast growth factor receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gastrulation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in hemocyte differentiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in hemocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc-derived wing morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc-derived wing vein specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in instar larval development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lamellocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in metamorphosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of apoptotic signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in photoreceptor cell development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in photoreceptor cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of ERK1 and ERK2 cascade	HGI more info	PubMed
involved_in positive regulation of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of photoreceptor cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein autophosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of cellular pH	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of multicellular organism growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sevenless signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in sevenless signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in terminal branching, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in terminal region determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in torso signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in torso signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vascular endothelial growth factor receptor signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in wing and notum subfield formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in wing disc morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: Raf oncogene

Names: CG2845-PA; CG2845-PE; Raf-PA; Raf-PE; l(1)pole hole; pole hole; pole-hole; polehole; raf-like-oncogene-1

NP_001096867.3

EC 2.7.11.1

EC 2.7.11.25

NP_525047.1

EC 2.7.11.1

EC 2.7.11.25

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_004354.4 Reference assembly

Range

2295466..2343870

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001103397.3 → NP_001096867.3 Raf oncogene, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001096867.3

Status: REVIEWED

UniProtKB/Swiss-Prot

A8JUV5, P11346, Q0KHW7, Q8I086, Q8I0D9, Q8ISE1, Q8ISE2, Q8ISE3, Q9NEH9, Q9W4Z3

Conserved Domains (3) summary

cd14062
Location:435 → 687

STKc_Raf; Catalytic domain of the Serine/Threonine Kinases, Raf (Rapidly Accelerated Fibrosarcoma) kinases

cd01816
Location:142 → 212

RBD_RAF; Ras-binding domain (RBD) found in RAF family serine/threonine kinases

cd20811
Location:221 → 269

C1_Raf; protein kinase C conserved region 1 (C1 domain) found in the Raf (Rapidly Accelerated Fibrosarcoma) kinase family
NM_080308.4 → NP_525047.1 Raf oncogene, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_525047.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A8JUV5, P11346, Q0KHW7, Q8I086, Q8I0D9, Q8ISE1, Q8ISE2, Q8ISE3, Q9NEH9, Q9W4Z3

Related

FBpp0070385

Conserved Domains (3) summary

cd14062
Location:435 → 687

STKc_Raf; Catalytic domain of the Serine/Threonine Kinases, Raf (Rapidly Accelerated Fibrosarcoma) kinases

cd01816
Location:142 → 212

RBD_RAF; Ras-binding domain (RBD) found in RAF family serine/threonine kinases

cd20811
Location:221 → 269

C1_Raf; protein kinase C conserved region 1 (C1 domain) found in the Raf (Rapidly Accelerated Fibrosarcoma) kinase family