Glrx3 glutaredoxin 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Glrx3provided by MGI
Official Full Name: glutaredoxin 3provided by MGI
Primary source: MGI:MGI:1353653
See related: Ensembl:ENSMUSG00000031068 AllianceGenome:MGI:1353653
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: PICOT; Txnl2
Summary: Predicted to enable identical protein binding activity and protein kinase C binding activity. Involved in negative regulation of cardiac muscle hypertrophy and regulation of the force of heart contraction. Located in Z disc. Is expressed in several structures, including brain; egg cylinder; genitourinary system; gut; and white fat. Orthologous to human GLRX3 (glutaredoxin 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in testis adult (RPKM 37.8), CNS E11.5 (RPKM 24.0) and 24 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7; 7 F4

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
109	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (137039302..137071360)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (137437573..137469631)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Liver specific disruption of Glutaredoxin 3 leads to iron accumulation and impaired cellular iron homeostasis.
Title: Liver specific disruption of Glutaredoxin 3 leads to iron accumulation and impaired cellular iron homeostasis.
Crucial Role of Mammalian Glutaredoxin 3 in Cardiac Energy Metabolism in Diet-induced Obese Mice Revealed by Transcriptome Analysis.
Title: Crucial Role of Mammalian Glutaredoxin 3 in Cardiac Energy Metabolism in Diet-induced Obese Mice Revealed by Transcriptome Analysis.
PICOT (GLRX3) is a positive regulator of stress-induced DNA-damage response.
Title: PICOT (GLRX3) is a positive regulator of stress-induced DNA-damage response.
Grx3 CKO mice exhibited increase of ROS production, impaired Ca2+ handling, left ventricular hypertrophy and heart failure.
Title: Cardiac-specific ablation of glutaredoxin 3 leads to cardiac hypertrophy and heart failure.
Grx3 is a key regulator of ROS in vivo and is involved in pregnancy-dependent mammary gland development and secretory activation through modulating cellular ROS.
Title: Loss of glutaredoxin 3 impedes mammary lobuloalveolar development during pregnancy and lactation.
Data show that protein kinase C-interacting cousin of thioredoxin (PICOT) alleviates myocardial I/R injury by regulating intracellular reactive oxygen species (ROS) and free iron levels, and suggest that PICOT presents a therapeutic strategy for myocardial I/R injury.
Title: PICOT alleviates myocardial ischemia-reperfusion injury by reducing intracellular levels of reactive oxygen species.
investigation of role of Grx3: embryos absent Grx3 were small, morphological defects, early embryonic lethality; mRNA ubiquitously expressed in developing embryos, adult tissues; induced during oxidative stress; necessary for cell cycle progression
Title: A mammalian monothiol glutaredoxin, Grx3, is critical for cell cycle progression during embryogenesis.
These data suggest that AM and PICOT might play cooperatively essential roles in embryogenesis as iron-sulfur cluster proteins.
Title: PICOT is a molecule which binds to anamorsin.
Demonstrated a differential expression of PICOT in various cell types, with a predominant cytosolic staining of epithelial cells and low or undetectable levels of PICOT in the stroma.
Title: Widespread expression of PICOT in mouse and human tissues with predominant localization to epithelium.
the precise PICOT protein level significantly affects the process of cardiac hypertrophy and cardiomyocyte contractility.
Title: PICOT is a critical regulator of cardiac hypertrophy and cardiomyocyte contractility.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1) 1 citation
Targeted (2) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D7Mit361 (e-PCR), detects polymorphism
- UniSTS:116749

RH126214 (e-PCR)
- UniSTS:162922

BE117517 (e-PCR)
- UniSTS:248695

NoName (e-PCR)
- UniSTS:234962

Homology

Orthologs from Annotation Pipeline: 490 organisms have orthologs with human gene Glrx3
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables iron-sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase C binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in [2Fe-2S] cluster assembly	ISO Inferred from Sequence Orthology more info
involved_in cell redox homeostasis	ISO Inferred from Sequence Orthology more info
involved_in intracellular iron ion homeostasis	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in iron-sulfur cluster assembly	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cardiac muscle hypertrophy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein maturation by iron-sulfur cluster transfer	ISO Inferred from Sequence Orthology more info
involved_in regulation of the force of heart contraction	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Z disc	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in dendrite	ISO Inferred from Sequence Orthology more info
part_of iron-sulfur cluster assembly complex	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: glutaredoxin-3

Names: PKC-interacting cousin of thioredoxin; PKC-theta-interacting protein; PKCq-interacting protein; thioredoxin-like protein 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001348093.1 → NP_001335022.1 glutaredoxin-3 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate exon in the 5' region and a downstream start codon, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1.

Source sequence(s)

AC131791

Conserved Domains (1) summary

cd03028
Location:93 → 181

GRX_PICOT_like; Glutaredoxin (GRX) family, PKC-interacting cousin of TRX (PICOT)-like subfamily; composed of PICOT and GRX-PICOT-like proteins. The non-PICOT members of this family contain only the GRX-like domain, whereas PICOT contains an N-terminal TRX-like domain ...
NM_023140.5 → NP_075629.2 glutaredoxin-3 isoform 1

See identical proteins and their annotated locations for NP_075629.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AC131791, AK017371, AK152446, BY771451

Consensus CDS

CCDS21948.1

UniProtKB/Swiss-Prot

Q9CQM9, Q9JLZ2

UniProtKB/TrEMBL

A0A8C6N241

Related

ENSMUSP00000066621.7, ENSMUST00000064404.8

Conserved Domains (2) summary

cd02984
Location:20 → 115

TRX_PICOT; TRX domain, PICOT (for PKC-interacting cousin of TRX) subfamily; PICOT is a protein that interacts with protein kinase C (PKC) theta, a calcium independent PKC isoform selectively expressed in skeletal muscle and T lymphocytes. PICOT contains an ...

cd03028
Location:139 → 227

GRX_PICOT_like; Glutaredoxin (GRX) family, PKC-interacting cousin of TRX (PICOT)-like subfamily; composed of PICOT and GRX-PICOT-like proteins. The non-PICOT members of this family contain only the GRX-like domain, whereas PICOT contains an N-terminal TRX-like domain ...