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robo1 roundabout, axon guidance receptor, homolog 1 (Drosophila) [ Danio rerio (zebrafish) ]

Gene ID: 30769, updated on 9-Sep-2025
Official Symbol
robo1provided by ZNC
Official Full Name
roundabout, axon guidance receptor, homolog 1 (Drosophila)provided by ZNC
Primary source
ZFIN:ZDB-GENE-000209-3
See related
Ensembl:ENSDARG00000026784 AllianceGenome:ZFIN:ZDB-GENE-000209-3
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Danio rerio
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as
SAG4A; t2gtl4a
Summary
Predicted to enable axon guidance receptor activity. Acts upstream of or within axon guidance and endocardial progenitor cell migration to the midline involved in heart field formation. Predicted to be located in membrane. Is expressed in basal plate midbrain region; epidermis; nervous system; pectoral fin; and pharyngeal arch. Human ortholog(s) of this gene implicated in autosomal recessive congenital nystagmus; combined or isolated pituitary hormone deficiency 8; congenital nystagmus; multiple myeloma; and plasmacytoma. Orthologous to human ROBO1 (roundabout guidance receptor 1). [provided by Alliance of Genome Resources, Apr 2025]
Orthologs
human mouse all
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See robo1 in Genome Data Viewer
Location:
chromosome: 15
Exon count:
36
Annotation release Status Assembly Chr Location
RS_2025_04 current GRCz12tu (GCF_049306965.1) 15 NC_133190.1 (41994344..42539581, complement)
RS_2024_08 previous assembly GRCz11 (GCF_000002035.6) 15 NC_007126.7 (39277653..39819970, complement)

Chromosome 15 - NC_133190.1Genomic Context describing neighboring genes Neighboring gene roundabout, axon guidance receptor, homolog 2 (Drosophila) Neighboring gene uncharacterized LOC137487783 Neighboring gene uncharacterized LOC141377910 Neighboring gene uncharacterized LOC137487784 Neighboring gene uncharacterized LOC137487785 Neighboring gene uncharacterized LOC137487786

Go to nucleotide: Graphics FASTA GenBank

  • Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
  • Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
  • BioProject: PRJEB1986
  • Analysis date: Fri Dec 8 19:48:10 2017

Related articles in PubMed

  1. Identification and characterization of roundabout orthologs in zebrafish. Challa AK, et al. Mech Dev, 2001 Mar. PMID 11231085
  2. Robo-Slit interactions regulate longitudinal axon pathfinding in the embryonic vertebrate brain. Devine CA, et al. Dev Biol, 2008 Jan 1. PMID 18061159
  3. Sox factors transcriptionally regulate ROBO4 gene expression in developing vasculature in zebrafish. Samant GV, et al. J Biol Chem, 2011 Sep 2. PMID 21730073, Free PMC Article
  4. A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish. Kawakami K, et al. Dev Cell, 2004 Jul. PMID 15239961
  5. Robo3 isoforms have distinct roles during zebrafish development. Challa AK, et al. Mech Dev, 2005 Oct. PMID 16129585

See all (29) citations in PubMed

GeneRIFs: Gene References Into Functions

Markers

Homology

Gene Ontology Provided by ZFIN

Function Evidence Code Pubs
enables axon guidance receptor activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within Roundabout signaling pathway IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within axon guidance IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within axon guidance IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within axon guidance IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within axon midline choice point recognition IEA
Inferred from Electronic Annotation
more info
  
involved_in central nervous system development IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within endocardial progenitor cell migration to the midline involved in heart field formation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in regulation of anatomical structure morphogenesis IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of multicellular organismal process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
roundabout homolog 1
Names
roundabout (axon guidance receptor, Drosophila) homolog 1
tol2 transposon-derived gene trap locus 4A

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001309824.3 → NP_001296753.2  roundabout homolog 1 isoform 3 precursor

    Status: VALIDATED

    Source sequence(s)
    JBMGRA010000015

  2. NM_001365166.2 → NP_001352095.1  roundabout homolog 1 isoform 1 precursor

    Status: VALIDATED

    Source sequence(s)
    JBMGRA010000015
    UniProtKB/TrEMBL
    A0A8M6Z4C2, A0AB13A9K0
    Conserved Domains (7) summary
    cd05725
    Location:282 → 349
    Ig3_Robo; Third immunoglobulin (Ig)-like domain in Robo (roundabout) receptors
    cd07693
    Location:70 → 169
    Ig1_Robo; First immunoglobulin (Ig)-like domain in Robo (roundabout) receptors and similar proteins
    cd00063
    Location:567 → 658
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    pfam00041
    Location:785 → 871
    fn3; Fibronectin type III domain
    pfam13927
    Location:460 → 534
    Ig_3; Immunoglobulin domain
    cl11960
    Location:371 → 460
    Ig; Immunoglobulin domain
    cl28033
    Location:1477 → 1639
    Herpes_ICP4_C; Herpesvirus ICP4-like protein C-terminal region

  3. NM_001365167.2 → NP_001352096.1  roundabout homolog 1 isoform 2 precursor

    Status: VALIDATED

    Source sequence(s)
    JBMGRA010000015
    UniProtKB/TrEMBL
    A0A8M6Z4C2, A0AB13A7G4
    Related
    ENSDARP00000087907.5, ENSDART00000097134.6
    Conserved Domains (7) summary
    cd05725
    Location:282 → 350
    Ig3_Robo; Third immunoglobulin (Ig)-like domain in Robo (roundabout) receptors
    cd07693
    Location:70 → 169
    Ig1_Robo; First immunoglobulin (Ig)-like domain in Robo (roundabout) receptors and similar proteins
    cd00063
    Location:564 → 655
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    pfam00041
    Location:782 → 868
    fn3; Fibronectin type III domain
    pfam13927
    Location:457 → 531
    Ig_3; Immunoglobulin domain
    cl11960
    Location:368 → 457
    Ig; Immunoglobulin domain
    cl28033
    Location:1473 → 1635
    Herpes_ICP4_C; Herpesvirus ICP4-like protein C-terminal region

RefSeqs of Annotated Genomes: GCF_049306965.1-RS_2025_04

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCz12tu Primary Assembly

Genomic

  1. NC_133190.1 Reference GRCz12tu Primary Assembly

    Range
    41994344..42539581 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_073922742.1 → XP_073778843.1  roundabout homolog 1 isoform X4

    UniProtKB/TrEMBL
    A0A8M6Z4C2, A0AB13A7G4

  2. XM_073922741.1 → XP_073778842.1  roundabout homolog 1 isoform X3

    UniProtKB/TrEMBL
    A0A8M6Z4C2, A0AB32T135

  3. XM_068213461.2 → XP_068069562.1  roundabout homolog 1 isoform X5

    UniProtKB/TrEMBL
    A0A8M6Z4C2, A0AB32T134

  4. XM_068213460.2 → XP_068069561.1  roundabout homolog 1 isoform X3

    UniProtKB/TrEMBL
    A0A8M6Z4C2, A0AB32T135

  5. XM_073922743.1 → XP_073778844.1  roundabout homolog 1 isoform X7

    UniProtKB/TrEMBL
    A0A8M6Z4C2

  6. XM_068213462.2 → XP_068069563.1  roundabout homolog 1 isoform X6

    UniProtKB/TrEMBL
    A0A8M6Z4C2, A0AB32T3U2

  7. XM_068213464.2 → XP_068069565.2  roundabout homolog 1 isoform X10

    UniProtKB/TrEMBL
    A0AB32T2D7

  8. XM_068213463.2 → XP_068069564.2  roundabout homolog 1 isoform X9

    UniProtKB/TrEMBL
    A0AB32T6R5

  9. XM_068213459.2 → XP_068069560.2  roundabout homolog 1 isoform X2

    UniProtKB/TrEMBL
    A0AB32T812

  10. XM_068213458.2 → XP_068069559.2  roundabout homolog 1 isoform X1

    UniProtKB/TrEMBL
    A0AB32T813

  11. XM_017359211.4 → XP_017214700.2  roundabout homolog 1 isoform X8

    UniProtKB/TrEMBL
    A0A8M6Z4C2

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_131481.1: Suppressed sequence

    Description
    NM_131481.1: This RefSeq was removed because currently there is insufficient support for the transcript and the protein.
Nucleotide Protein Strain
Heading Accession and Version

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