Clic4 chloride intracellular channel 4 (mitochondrial) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Clic4provided by MGI
Official Full Name: chloride intracellular channel 4 (mitochondrial)provided by MGI
Primary source: MGI:MGI:1352754
See related: Ensembl:ENSMUSG00000037242 AllianceGenome:MGI:1352754
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: TU-74; mc3s5; D0Jmb3; mtCLIC
Summary: Predicted to enable chloride channel activity. Involved in keratinocyte differentiation. Acts upstream of or within several processes, including endothelial cell morphogenesis; vacuolar acidification; and vasculature development. Located in mitochondrion. Is expressed in brain; skin; and spleen. Orthologous to human CLIC4 (chloride intracellular channel 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in lung adult (RPKM 93.5), bladder adult (RPKM 63.6) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 4; 4 D3

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
109	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (134941280..135000071, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (135213969..135272760, complement)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The oxidoreductase CLIC4 is required to maintain mitochondrial function and resistance to exogenous oxidants in breast cancer cells.
Title: The oxidoreductase CLIC4 is required to maintain mitochondrial function and resistance to exogenous oxidants in breast cancer cells.
Chloride intracellular channel 4 (CLIC4) expression profile in the mouse medial prefrontal cortex and its regulation by ethanol.
Title: Chloride intracellular channel 4 (CLIC4) expression profile in the mouse medial prefrontal cortex and its regulation by ethanol.
Retinal pigment epithelium-specific CLIC4 mutant is a mouse model of dry age-related macular degeneration.
Title: Retinal pigment epithelium-specific CLIC4 mutant is a mouse model of dry age-related macular degeneration.
CLIC1 and CLIC4 mediate endothelial S1P receptor signaling to facilitate Rac1 and RhoA activity and function.
Title: CLIC1 and CLIC4 mediate endothelial S1P receptor signaling to facilitate Rac1 and RhoA activity and function.
Results indicate CLICs (CLIC1, CLIC4 and CLIC5)-dependent chloride efflux as an essential and proximal upstream event for NLRP3 activation.
Title: CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation.
Data, including data from studies in primary cells from knockout mice, suggest Clic1 and Clic4 participate in signaling interleukin 1beta secretion and in activation of Nlrp3 inflammasomes; in LPS macrophage activation, Clic1 and Clic4 are translocated to nucleus/cell membranes. (Clic1 = chloride intracellular channel 1; Clic4 = chloride intracellular channel 4, mitochondrial; Nlrp3 = NLR family pyrin domain containing 3)
Title: The intracellular chloride channel proteins CLIC1 and CLIC4 induce IL-1β transcription and activate the NLRP3 inflammasome.
study reports an alternately-spliced form of Smad7, Smad7Delta, that is induced by TGF-beta and CLIC4, is a dominant inhibitor of Smad7 and enhances TGF-beta signaling
Title: Elevating CLIC4 in Multiple Cell Types Reveals a TGF- Dependent Induction of a Dominant Negative Smad7 Splice Variant.
The findings indicate that CLIC4/CLIC5A-mediated ERM activation is required for maintenance of the glomerular capillary architecture.
Title: Both CLIC4 and CLIC5A activate ERM proteins in glomerular endothelium.
CLIC4 is not required for collaterogenesis but is essential for perinatal maturation of nascent collaterals through a mechanism that supports VEGF signaling.
Title: Chloride intracellular channel 4 is required for maturation of the cerebral collateral circulation.
However, the absence of CLIC4 has no significant impact on the extent of functional recovery or fibrosis following acute injury.
Title: Absence of chloride intracellular channel 4 (CLIC4) predisposes to acute kidney injury but has minimal impact on recovery.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (2)
Targeted (5) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D11Bhm20 (e-PCR), detects polymorphism
- UniSTS:141619

D9Mit34.1 (e-PCR), detects polymorphism
- UniSTS:131903

Homology

Homologs of the Clic4 gene: The Clic4 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, zebrafish, and frog.
Orthologs from Annotation Pipeline: 474 organisms have orthologs with human gene Clic4
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables chloride channel activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables chloride channel activity	ISO Inferred from Sequence Orthology more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables voltage-gated monoatomic ion channel activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within angiogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within branching morphogenesis of an epithelial tube	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to calcium ion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within chloride transmembrane transport	ISO Inferred from Sequence Orthology more info	PubMed
involved_in chloride transport	IBA Inferred from Biological aspect of Ancestor more info	PubMed
acts_upstream_of_or_within chloride transport	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within endothelial cell morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within fertilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in keratinocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in keratinocyte differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within monoatomic ion transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within multicellular organism growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell migration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of monoatomic ion transmembrane transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within retina vasculature morphogenesis in camera-type eye	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within vacuolar acidification	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in apical part of cell	ISO Inferred from Sequence Orthology more info
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in cell-cell junction	ISO Inferred from Sequence Orthology more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
part_of chloride channel complex	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in membrane	ISS Inferred from Sequence or Structural Similarity more info	PubMed
colocalizes_with microtubule cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in microvillus	ISO Inferred from Sequence Orthology more info
located_in midbody	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in nuclear matrix	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: chloride intracellular channel protein 4

Names: mc3s5/mtCLIC

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.