U.S. flag

An official website of the United States government


Send to:

Choose Destination

PDIA3 protein disulfide isomerase family A member 3 [ Homo sapiens (human) ]

Gene ID: 2923, updated on 6-Nov-2022


Official Symbol
PDIA3provided by HGNC
Official Full Name
protein disulfide isomerase family A member 3provided by HGNC
Primary source
See related
Ensembl:ENSG00000167004 MIM:602046; AllianceGenome:HGNC:4606
Gene type
protein coding
RefSeq status
Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. This protein also functions as a molecular chaperone that prevents the formation of protein aggregates. [provided by RefSeq, Dec 2016]
Ubiquitous expression in thyroid (RPKM 333.0), prostate (RPKM 175.9) and 25 other tissues See more
Try the new Gene table
Try the new Transcript table

Genomic context

See PDIA3 in Genome Data Viewer
Exon count:
Annotation release Status Assembly Chr Location
110 current GRCh38.p14 (GCF_000001405.40) 15 NC_000015.10 (43746438..43773278)
110 current T2T-CHM13v2.0 (GCF_009914755.1) 15 NC_060939.1 (41553795..41580638)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 15 NC_000015.9 (44038636..44065476)

Chromosome 15 - NC_000015.10Genomic Context describing neighboring genes Neighboring gene RNA, U6 small nuclear 354, pseudogene Neighboring gene cation channel sperm associated 2 pseudogene 1 Neighboring gene elongation factor for RNA polymerase II 3 Neighboring gene small EDRK-rich factor 2 Neighboring gene SERF2-C15orf63 readthrough Neighboring gene microRNA 1282 Neighboring gene serine incorporator 4

Genomic regions, transcripts, and products


  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018


GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
Knockdown of protein disulfide isomerase family A, member 3 (PDIA3) by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 gp120/MBL complex co-localizes with the ER marker ERp57 and the Golgi marker p230 at subcellular perinuclear compartments in neuronal cells PubMed
env PDI is predominantly involved in HIV-1 entry and infection of the T cell line PM-1 and PHA-stimulated primary T lymphocytes, suggesting the preferential use of PDI relevant to the HIV-1 entry and establishment of virus reservoirs in resting CD4+ cells PubMed
env The disulfide cross-linking interaction between gp120 and PDI is enhanced by CD4 protein PubMed
env Protein-disulfide isomerase (PDI) cleaves disulfide bonds in recombinant HIV-1 envelope glycoprotein gp120, and gp120 bound to the surface receptor CD4 undergoes a disulfide reduction that is prevented by PDI inhibitors PubMed
Envelope surface glycoprotein gp160, precursor env HIV-1 gp160 interacts with PDIA3; predicted interaction to be within the endoplasmic reticulum and function as a thioredoxin reductase PubMed
env HIV-1 gp160 is identified to have a physical interaction with protein disulfide isomerase family A, member 3 (PDIA3) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
env Treatment of trimeric HIV-1 rgp140 with protein disulfide isomerase yields monomers by disruption of the intermolecular disulfide bonds PubMed
Envelope transmembrane glycoprotein gp41 env HIV-1 gp41 is identified to have a physical interaction with protein disulfide isomerase family A, member 3 (PDIA3) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
Vpr vpr A stable-isotope labeling by amino acids in cell culture coupled with mass spectrometry-based proteomics identifies downregulation of protein disulfide isomerase A3 (PDIA3) expression by HIV-1 Vpr in Vpr transduced macrophages PubMed
retropepsin gag-pol Positional proteomics analysis identifies the cleavage of human protein disulfide isomerase family A, member 3 (PDIA3) at amino acid residues 27-28 by the HIV-1 protease PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem


Products Interactant Other Gene Complex Source Pubs Description

General gene information



Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables RNA binding HDA PubMed 
enables cysteine-type endopeptidase activity TAS
Traceable Author Statement
more info
enables disulfide oxidoreductase activity TAS
Traceable Author Statement
more info
enables identical protein binding IPI
Inferred from Physical Interaction
more info
enables phospholipase C activity TAS
Traceable Author Statement
more info
enables protein binding IPI
Inferred from Physical Interaction
more info
enables protein disulfide isomerase activity IBA
Inferred from Biological aspect of Ancestor
more info
enables protein-disulfide reductase activity IDA
Inferred from Direct Assay
more info

General protein information

Preferred Names
protein disulfide-isomerase A3
58 kDa glucose-regulated protein
58 kDa microsomal protein
ER protein 57
ER protein 60
disulfide isomerase ER-60
endoplasmic reticulum P58
endoplasmic reticulum resident protein 57
endoplasmic reticulum resident protein 60
epididymis secretory protein Li 269
epididymis secretory sperm binding protein Li 93n
glucose regulated protein, 58kDa
phospholipase C-alpha
protein disulfide isomerase-associated 3

NCBI Reference Sequences (RefSeq)

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_005313.5NP_005304.3  protein disulfide-isomerase A3 precursor

    See identical proteins and their annotated locations for NP_005304.3

    Status: REVIEWED

    Source sequence(s)
    AC018512, AI769725, BC014433, BE644608, BG826781, BM854408
    Consensus CDS
    P30101, Q9UMU7
    ENSP00000300289.5, ENST00000300289.10
    Conserved Domains (1) summary
    YbbN; Negative regulator of GroEL, contains thioredoxin-like and TPR-like domains [Posttranslational modification, protein turnover, chaperones]

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 110 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly


  1. NC_000015.10 Reference GRCh38.p14 Primary Assembly

    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0


  1. NC_060939.1 Alternate T2T-CHM13v2.0

    GenBank, FASTA, Sequence Viewer (Graphics)