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DROSHA drosha ribonuclease III [ Homo sapiens (human) ]

Gene ID: 29102, updated on 28-Oct-2018

Summary

Official Symbol
DROSHAprovided by HGNC
Official Full Name
drosha ribonuclease IIIprovided by HGNC
Primary source
HGNC:HGNC:17904
See related
Ensembl:ENSG00000113360 MIM:608828; Vega:OTTHUMG00000161976
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
RN3; ETOHI2; RNASEN; RANSE3L; RNASE3L; HSA242976
Summary
This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016]
Expression
Ubiquitous expression in brain (RPKM 9.5), ovary (RPKM 7.6) and 25 other tissues See more
Orthologs

Genomic context

See DROSHA in Genome Data Viewer
Location:
5p13.3
Exon count:
39
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) 5 NC_000005.10 (31400494..31532175, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 5 NC_000005.9 (31400601..31532282, complement)

Chromosome 5 - NC_000005.10Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC105374709 Neighboring gene cadherin 6 Neighboring gene double homeobox 4 like 51 (pseudogene) Neighboring gene chromosome 5 open reading frame 22 Neighboring gene RNA, U6 small nuclear 363, pseudogene

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Jun 15 11:32:44 2016

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Pr55(Gag) gag Knockdown of Drosha by shRNA leads to inhibit enrichment of HIV-1 Gag in intracellular vacuoles, but enhance viral particle release in Gag expressing cells PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

  • DDX1 as a regulatory component of the Drosha microprocessor, organism-specific biosystem (from WikiPathways)
    DDX1 as a regulatory component of the Drosha microprocessor, organism-specific biosystemPosttranscriptional maturation is a critical step in microRNA (miRNA) biogenesis that determines mature miRNA levels. In addition to core components (Drosha and DGCR8 [DiGeorge syndrome critical regi...
  • Direct p53 effectors, organism-specific biosystem (from Pathway Interaction Database)
    Direct p53 effectors, organism-specific biosystem
    Direct p53 effectors
  • Gene Expression, organism-specific biosystem (from REACTOME)
    Gene Expression, organism-specific biosystemGene Expression covers the pathways by which genomic DNA is transcribed to yield RNA, the regulation of these transcription processes, and the pathways by which newly-made RNA Transcripts are process...
  • Gene Silencing by RNA, organism-specific biosystem (from REACTOME)
    Gene Silencing by RNA, organism-specific biosystemIn this module, the biology of various types of regulatory non-coding RNAs are described. Currently, biogenesis and functions of small interfering RNAs (siRNAs) and microRNAs are annotated.
  • MicroRNA (miRNA) biogenesis, organism-specific biosystem (from REACTOME)
    MicroRNA (miRNA) biogenesis, organism-specific biosystemBiogenesis of microRNAs (miRNAs) can be summarized in five steps (reviewed in Ketting 2011, Nowotny and Yang 2009, Kim et al. 2009, Chua et al. 2009, Hannon and He 2004):1. Transcription. miRNA trans...
  • Proteoglycans in cancer, organism-specific biosystem (from KEGG)
    Proteoglycans in cancer, organism-specific biosystemMany proteoglycans (PGs) in the tumor microenvironment have been shown to be key macromolecules that contribute to biology of various types of cancer including proliferation, adhesion, angiogenesis a...
  • Proteoglycans in cancer, conserved biosystem (from KEGG)
    Proteoglycans in cancer, conserved biosystemMany proteoglycans (PGs) in the tumor microenvironment have been shown to be key macromolecules that contribute to biology of various types of cancer including proliferation, adhesion, angiogenesis a...
  • RNA interference, organism-specific biosystem (from WikiPathways)
    RNA interference, organism-specific biosystemThe process of RNA-interference in eukaryotic cells. Long precursor microRNA (miRNA), called pri-miRNA, is cleaved by RNase III endonuclease (Drosha) into pieces of approximately 70 nucleotides each ...
  • Ribosome biogenesis in eukaryotes, organism-specific biosystem (from KEGG)
    Ribosome biogenesis in eukaryotes, organism-specific biosystemRibosomes are the cellular factories responsible for making proteins. In eukaryotes, ribosome biogenesis involves the production and correct assembly of four rRNAs and about 80 ribosomal proteins. It...
  • Ribosome biogenesis in eukaryotes, conserved biosystem (from KEGG)
    Ribosome biogenesis in eukaryotes, conserved biosystemRibosomes are the cellular factories responsible for making proteins. In eukaryotes, ribosome biogenesis involves the production and correct assembly of four rRNAs and about 80 ribosomal proteins. It...
  • miRNA Biogenesis, organism-specific biosystem (from WikiPathways)
    miRNA Biogenesis, organism-specific biosystemmicroRNA biogenesis + an example of a miRBase entry. As an example immature hsa-mir-146a is shown

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
DEAD/H-box RNA helicase binding IPI
Inferred from Physical Interaction
more info
PubMed 
R-SMAD binding IPI
Inferred from Physical Interaction
more info
PubMed 
RNA binding HDA PubMed 
SMAD binding IPI
Inferred from Physical Interaction
more info
PubMed 
double-stranded RNA binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
lipopolysaccharide binding IDA
Inferred from Direct Assay
more info
PubMed 
metal ion binding IEA
Inferred from Electronic Annotation
more info
 
primary miRNA binding IPI
Inferred from Physical Interaction
more info
PubMed 
primary miRNA binding NAS
Non-traceable Author Statement
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein homodimerization activity IDA
Inferred from Direct Assay
more info
PubMed 
ribonuclease III activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
ribonuclease III activity IDA
Inferred from Direct Assay
more info
PubMed 
Process Evidence Code Pubs
RNA phosphodiester bond hydrolysis, endonucleolytic IEA
Inferred from Electronic Annotation
more info
 
RNA processing IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
defense response to Gram-negative bacterium IDA
Inferred from Direct Assay
more info
PubMed 
defense response to Gram-positive bacterium IDA
Inferred from Direct Assay
more info
PubMed 
miRNA metabolic process IEA
Inferred from Electronic Annotation
more info
 
positive regulation of gene expression IEA
Inferred from Electronic Annotation
more info
 
pre-miRNA processing IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
primary miRNA processing IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
primary miRNA processing IDA
Inferred from Direct Assay
more info
PubMed 
primary miRNA processing IMP
Inferred from Mutant Phenotype
more info
PubMed 
production of siRNA involved in RNA interference IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
rRNA catabolic process IEA
Inferred from Electronic Annotation
more info
 
regulation of inflammatory response IEA
Inferred from Electronic Annotation
more info
 
regulation of miRNA metabolic process IEA
Inferred from Electronic Annotation
more info
 
regulation of regulatory T cell differentiation IEA
Inferred from Electronic Annotation
more info
 
ribosome biogenesis IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
microprocessor complex IDA
Inferred from Direct Assay
more info
PubMed 
nucleolus IEA
Inferred from Electronic Annotation
more info
 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
postsynaptic density IEA
Inferred from Electronic Annotation
more info
 

General protein information

Preferred Names
ribonuclease 3
Names
RNase III
drosha, double-stranded RNA-specific endoribonuclease
nuclear RNase III Drosha
p241
putative protein p241 which interacts with transcription factor Sp1
putative ribonuclease III
ribonuclease type III, nuclear
NP_001093882.1
NP_037367.3
XP_005248348.1
XP_005248349.1
XP_005248350.1
XP_005248351.1
XP_011512335.1
XP_016864888.1
XP_016864889.1
XP_016864890.1
XP_016864891.1
XP_024301801.1
XP_024301802.1
XP_024301803.1
XP_024301804.1

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_051574.1 RefSeqGene

    Range
    5001..136682
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001100412.1NP_001093882.1  ribonuclease 3 isoform 2

    See identical proteins and their annotated locations for NP_001093882.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter than isoform 1.
    Source sequence(s)
    AA917625, AC008768, BC024261, BX647724, DB065470
    Consensus CDS
    CCDS47194.1
    UniProtKB/Swiss-Prot
    Q9NRR4
    Related
    ENSP00000424161.1, OTTHUMP00000220912, ENST00000513349.5, OTTHUMT00000366562
    Conserved Domains (3) summary
    cd00593
    Location:9221042
    RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
    pfam08648
    Location:230357
    DUF1777; Protein of unknown function (DUF1777)
    cl25983
    Location:10681296
    Rnc; dsRNA-specific ribonuclease [Transcription]
  2. NM_013235.4NP_037367.3  ribonuclease 3 isoform 1

    See identical proteins and their annotated locations for NP_037367.3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AA917625, AC008768, AF189011, BC024261, DB065470
    Consensus CDS
    CCDS47195.1
    UniProtKB/Swiss-Prot
    Q9NRR4
    Related
    ENSP00000425979.2, OTTHUMP00000220911, ENST00000511367.6, OTTHUMT00000366561
    Conserved Domains (3) summary
    cd00593
    Location:9591079
    RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
    pfam08648
    Location:230317
    DUF1777; Protein of unknown function (DUF1777)
    cl25983
    Location:11051333
    Rnc; dsRNA-specific ribonuclease [Transcription]

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000005.10 Reference GRCh38.p12 Primary Assembly

    Range
    31400494..31532175 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017009401.2XP_016864890.1  ribonuclease 3 isoform X8

  2. XM_005248294.4XP_005248351.1  ribonuclease 3 isoform X7

    See identical proteins and their annotated locations for XP_005248351.1

    UniProtKB/Swiss-Prot
    Q9NRR4
    Conserved Domains (5) summary
    smart00358
    Location:11931264
    DSRM; Double-stranded RNA binding motif
    PRK00102
    Location:10371265
    rnc; ribonuclease III; Reviewed
    cd00593
    Location:10541186
    RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
    pfam08648
    Location:219332
    DUF1777; Protein of unknown function (DUF1777)
    pfam12871
    Location:218296
    PRP38_assoc; Pre-mRNA-splicing factor 38-associated hydrophilic C-term
  3. XM_017009399.2XP_016864888.1  ribonuclease 3 isoform X5

  4. XM_005248293.4XP_005248350.1  ribonuclease 3 isoform X3

    Conserved Domains (5) summary
    smart00358
    Location:12301301
    DSRM; Double-stranded RNA binding motif
    PRK00102
    Location:10741302
    rnc; ribonuclease III; Reviewed
    cd00593
    Location:10911223
    RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
    pfam08648
    Location:219286
    DUF1777; Protein of unknown function (DUF1777)
    pfam12871
    Location:219280
    PRP38_assoc; Pre-mRNA-splicing factor 38-associated hydrophilic C-term
  5. XM_017009400.2XP_016864889.1  ribonuclease 3 isoform X6

    Conserved Domains (3) summary
    cd00593
    Location:9141034
    RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
    pfam08648
    Location:230357
    DUF1777; Protein of unknown function (DUF1777)
    cl25983
    Location:10601288
    Rnc; dsRNA-specific ribonuclease [Transcription]
  6. XM_005248292.4XP_005248349.1  ribonuclease 3 isoform X2

    Conserved Domains (5) summary
    smart00358
    Location:12531324
    DSRM; Double-stranded RNA binding motif
    PRK00102
    Location:10971325
    rnc; ribonuclease III; Reviewed
    cd00593
    Location:11141246
    RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
    pfam08648
    Location:217310
    DUF1777; Protein of unknown function (DUF1777)
    pfam12871
    Location:219278
    PRP38_assoc; Pre-mRNA-splicing factor 38-associated hydrophilic C-term
  7. XM_005248291.4XP_005248348.1  ribonuclease 3 isoform X1

    See identical proteins and their annotated locations for XP_005248348.1

    UniProtKB/Swiss-Prot
    Q9NRR4
    Conserved Domains (3) summary
    cd00593
    Location:9591079
    RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
    pfam08648
    Location:230317
    DUF1777; Protein of unknown function (DUF1777)
    cl25983
    Location:11051333
    Rnc; dsRNA-specific ribonuclease [Transcription]
  8. XM_024446035.1XP_024301803.1  ribonuclease 3 isoform X4

    Conserved Domains (3) summary
    cd00593
    Location:9221042
    RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
    pfam08648
    Location:230357
    DUF1777; Protein of unknown function (DUF1777)
    cl25983
    Location:10681296
    Rnc; dsRNA-specific ribonuclease [Transcription]
  9. XM_024446033.1XP_024301801.1  ribonuclease 3 isoform X1

    Conserved Domains (3) summary
    cd00593
    Location:9591079
    RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
    pfam08648
    Location:230317
    DUF1777; Protein of unknown function (DUF1777)
    cl25983
    Location:11051333
    Rnc; dsRNA-specific ribonuclease [Transcription]
  10. XM_024446034.1XP_024301802.1  ribonuclease 3 isoform X1

    Conserved Domains (3) summary
    cd00593
    Location:9591079
    RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
    pfam08648
    Location:230317
    DUF1777; Protein of unknown function (DUF1777)
    cl25983
    Location:11051333
    Rnc; dsRNA-specific ribonuclease [Transcription]
  11. XM_017009402.1XP_016864891.1  ribonuclease 3 isoform X11

  12. XM_011514033.3XP_011512335.1  ribonuclease 3 isoform X9

    Conserved Domains (3) summary
    cd00593
    Location:9591079
    RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
    pfam08648
    Location:217310
    DUF1777; Protein of unknown function (DUF1777)
    pfam12871
    Location:219278
    PRP38_assoc; Pre-mRNA-splicing factor 38-associated hydrophilic C-term
  13. XM_024446036.1XP_024301804.1  ribonuclease 3 isoform X10

    Conserved Domains (2) summary
    pfam08648
    Location:218314
    DUF1777; Protein of unknown function (DUF1777)
    cl25983
    Location:879979
    Rnc; dsRNA-specific ribonuclease [Transcription]
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