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thiS sulfur carrier protein ThiS [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 2847702, updated on 3-Jun-2026
Official Symbol
thiS
Official Full Name
sulfur carrier protein ThiS
Primary source
ECOCYC:G1
Locus tag
b4407
See related
ASAP:ABE-0023907
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3983; thiG1
Summary
The ThiS N-terminus has been verified by MS/MS sequencing, the Met is not clipped and the measured mass is 7310.74 (Kelleher, 1998). [More information is available at EcoGene: EG14363]. ThiS is the sulfur source for the thiazole moiety in thiamin biosynthesis. [More information is available at EcoCyc: G1].
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Sequence:
NC_000913.3 (4192637..4192837, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene 2-iminoacetate synthase Neighboring gene 1-deoxy-D-xylulose 5-phosphate:thiol sulfurtransferase Neighboring gene sulfur carrier protein ThiS adenylyltransferase Neighboring gene thiamine phosphate synthase Neighboring gene phosphomethylpyrimidine synthase

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Overexpression of recombinant proteins with a C-terminal thiocarboxylate: implications for protein semisynthesis and thiamin biosynthesis. Kinsland C, et al. Protein Sci, 1998 Aug. PMID 10082383, Free PMC Article
  2. Heat shock transcription factor δ³² is targeted for degradation via an ubiquitin-like protein ThiS in Escherichia coli. Xu X, et al. Biochem Biophys Res Commun, 2015 Apr 3. PMID 25721662
  3. Structure of the Escherichia coli ThiS-ThiF complex, a key component of the sulfur transfer system in thiamin biosynthesis. Lehmann C, et al. Biochemistry, 2006 Jan 10. PMID 16388576, Free PMC Article
  4. The enzymology of sulfur activation during thiamin and biotin biosynthesis. Begley TP, et al. Curr Opin Chem Biol, 1999 Oct. PMID 10508664
  5. Thiamin biosynthesis in Escherichia coli. Identification of ThiS thiocarboxylate as the immediate sulfur donor in the thiazole formation. Taylor SV, et al. J Biol Chem, 1998 Jun 26. PMID 9632726

See all (15) citations in PubMed

GeneRIFs: Gene References Into Functions

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables sulfur carrier activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables sulfur carrier activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in thiamine diphosphate biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in thiazole biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within thiazole biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
part_of adenylyltransferase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of adenylyltransferase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of sulfurtransferase complex NAS
Non-traceable Author Statement
more info
 PubMed 
Preferred Names
sulfur carrier protein ThiS

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4192637..4192837 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. YP_026279.1 sulfur carrier protein ThiS

    See identical proteins and their annotated locations for YP_026279.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A8T5ZBV6
    Conserved Domains (1) summary
    PRK08053
    Location:1 → 66
    PRK08053; sulfur carrier protein ThiS; Provisional
Nucleotide Protein
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