Amot angiomotin [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Amotprovided by MGI
Official Full Name: angiomotinprovided by MGI
Primary source: MGI:MGI:108440
See related: Ensembl:ENSMUSG00000041688 AllianceGenome:MGI:108440
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Sii6; CAG-2; D0Kist1
Summary: Predicted to enable angiostatin binding activity and signaling receptor activity. Acts upstream of or within several processes, including ameboidal-type cell migration; negative regulation of GTPase activity; and vasculogenesis. Located in bicellular tight junction; cytoplasmic vesicle; and lamellipodium. Is expressed in several structures, including egg cylinder; embryo endoderm; embryo mesoderm; and mesendoderm. Orthologous to human AMOT (angiomotin). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in limb E14.5 (RPKM 14.2), genital fat pad adult (RPKM 12.4) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: X; X F2

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
109	current	GRCm39 (GCF_000001635.27)	X	NC_000086.8 (144229420..144288962, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	X	NC_000086.7 (145446424..145505966, complement)

Chromosome X - NC_000086.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Sequestration of eIF4A by angiomotin: A novel mechanism to restrict global protein synthesis in trophoblast cells.
Title: Sequestration of eIF4A by angiomotin: A novel mechanism to restrict global protein synthesis in trophoblast cells.
Amot and Yap1 regulate dendrite development by affecting the phosphorylation of S6 kinase and its target S6 ribosomal protein.
Title: Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice.
Collectively, we have uncovered that AMOT acts as a YAP stimulator in high glucose level.
Title: AMOT is required for YAP function in high glucose induced liver malignancy.
Rho attenuates the interaction between Amot and Nf2 by binding to the coiled-coil domain of Amot.
Title: Rho differentially regulates the Hippo pathway by modulating the interaction between Amot and Nf2 in the blastocyst.
TFPI-1 interacts with AMOT, which led to a decrease in the phosphorylation of YAP and further increased the genes expression of the proliferation and migration involved. Our results further confirmed that atherosclerosis was a localized disease.
Title: Conditional knockout of TFPI-1 in VSMCs of mice accelerates atherosclerosis by enhancing AMOT/YAP pathway.
a new function of RNF146 and tankyrase in stabilizing the Crumbs complex through downregulation of AMOT proteins at the apical membrane, is reported.
Title: The RNF146 and tankyrase pathway maintains the junctional Crumbs complex through regulation of angiomotin.
AMOT is a crucial suppressor of lung cancer metastasis and highlight its critical role as a tumor suppressor and its potential as a prognostic biomarker and therapeutic target for lung cancer.
Title: Angiomotin decreases lung cancer progression by sequestering oncogenic YAP/TAZ and decreasing Cyr61 expression.
Within the nucleus, Amot-p130 was associated with the transcriptional complex containing Yap and Teads (TEA domain family members) and contributed to the regulation of a subset of Yap target genes, many of which are associated with tumorigenesis.
Title: The p130 isoform of angiomotin is required for Yap-mediated hepatic epithelial cell proliferation and tumorigenesis.
The loss of Angiomotin, together with Angiomotin-like 2, leads to differentiation of inner cell mass cells and compromised peri-implantation development.
Title: Angiomotin prevents pluripotent lineage differentiation in mouse embryos via Hippo pathway-dependent and -independent mechanisms.
The phosphorylation of S176 in the N-terminal domain of Amot is a critical step for activation of the Hippo pathway in adherens junctions and cell polarity disconnects the Hippo pathway from cell-cell adhesion by sequestering Amot from AJs.
Title: Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH127083 (e-PCR)
- UniSTS:209545

Amot (e-PCR), detects polymorphism
- UniSTS:502740

Homology

Homologs of the Amot gene: The Amot gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, zebrafish, and frog.
Orthologs from Annotation Pipeline: 340 organisms have orthologs with human gene Amot
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables angiostatin binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables signaling receptor activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables signaling receptor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info	PubMed
acts_upstream_of_or_within actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in angiogenesis	IBA Inferred from Biological aspect of Ancestor more info	PubMed
acts_upstream_of_or_within blood vessel endothelial cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within blood vessel morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell migration involved in gastrulation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within chemotaxis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of embryo development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of cell polarity involved in ameboidal cell migration	IBA Inferred from Biological aspect of Ancestor more info	PubMed
acts_upstream_of_or_within establishment of cell polarity involved in ameboidal cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within establishment of epithelial cell polarity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within gastrulation with mouth forming second	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in hippo signaling	IBA Inferred from Biological aspect of Ancestor more info	PubMed
acts_upstream_of_or_within hippo signaling	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within in utero embryonic development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of GTPase activity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of vascular permeability	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of blood vessel endothelial cell migration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of embryonic development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein localization	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell migration	IBA Inferred from Biological aspect of Ancestor more info	PubMed
acts_upstream_of_or_within regulation of cell migration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of small GTPase mediated signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within vasculogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of COP9 signalosome	ISO Inferred from Sequence Orthology more info
located_in actin filament	ISO Inferred from Sequence Orthology more info
is_active_in bicellular tight junction	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in bicellular tight junction	IDA Inferred from Direct Assay more info	PubMed
located_in bicellular tight junction	ISO Inferred from Sequence Orthology more info
located_in cell junction	ISO Inferred from Sequence Orthology more info
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasmic vesicle	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in cytoplasmic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in endocytic vesicle	ISO Inferred from Sequence Orthology more info
located_in external side of plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in lamellipodium	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in lamellipodium	IDA Inferred from Direct Assay more info	PubMed
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in ruffle	ISO Inferred from Sequence Orthology more info
located_in stress fiber	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: angiomotin

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001290274.2 → NP_001277203.1 angiomotin isoform 2

Status: VALIDATED

Source sequence(s)

AL807753

UniProtKB/Swiss-Prot

Q8VHG2

Conserved Domains (2) summary

COG1196
Location:367 → 664

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

pfam12240
Location:578 → 785

Angiomotin_C; Angiomotin C terminal
NM_001403386.1 → NP_001390315.1 angiomotin isoform 3

Status: VALIDATED

Source sequence(s)

AL807753

UniProtKB/TrEMBL

A0A067XG49
NM_001403387.1 → NP_001390316.1 angiomotin isoform 1

Status: VALIDATED

Source sequence(s)

AL807753

UniProtKB/Swiss-Prot

Q6ZPZ1
NM_001403388.1 → NP_001390317.1 angiomotin isoform 1

Status: VALIDATED

Source sequence(s)

AL807753

UniProtKB/Swiss-Prot

Q6ZPZ1
NM_001403389.1 → NP_001390318.1 angiomotin isoform 3

Status: VALIDATED

Source sequence(s)

AL807753

UniProtKB/TrEMBL

A0A067XG49
NM_153319.4 → NP_695231.3 angiomotin isoform 1

See identical proteins and their annotated locations for NP_695231.3

Status: VALIDATED

Source sequence(s)

AL807753

Consensus CDS

CCDS72443.1

UniProtKB/Swiss-Prot

Q6ZPZ1, Q8VHG2

Related

ENSMUSP00000108455.3, ENSMUST00000112836.9

Conserved Domains (4) summary

PRK05641
Location:1017 → 1049

PRK05641; putative acetyl-CoA carboxylase biotin carboxyl carrier protein subunit; Validated

PRK07764
Location:1014 → 1098

PRK07764; DNA polymerase III subunits gamma and tau; Validated

pfam02463
Location:418 → 669

SMC_N; RecF/RecN/SMC N terminal domain

pfam12240
Location:578 → 781

Angiomotin_C; Angiomotin C terminal

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000086.8 Reference GRCm39 C57BL/6J

Range

144229420..144288962 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030251360.1 → XP_030107220.1 angiomotin isoform X4

Related

ENSMUSP00000108454.2, ENSMUST00000112835.8

Conserved Domains (4) summary

COG1196
Location:19 → 275

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

PRK05641
Location:628 → 660

PRK05641; putative acetyl-CoA carboxylase biotin carboxyl carrier protein subunit; Validated

PRK07764
Location:625 → 709

PRK07764; DNA polymerase III subunits gamma and tau; Validated

pfam12240
Location:189 → 392

Angiomotin_C; Angiomotin C terminal
XM_011247828.3 → XP_011246130.1 angiomotin isoform X1

UniProtKB/Swiss-Prot

Q8VHG2

Conserved Domains (4) summary

PRK05641
Location:1017 → 1049

PRK05641; putative acetyl-CoA carboxylase biotin carboxyl carrier protein subunit; Validated

PRK07764
Location:1014 → 1098

PRK07764; DNA polymerase III subunits gamma and tau; Validated

pfam02463
Location:418 → 669

SMC_N; RecF/RecN/SMC N terminal domain

pfam12240
Location:578 → 781

Angiomotin_C; Angiomotin C terminal
XM_006528864.4 → XP_006528927.1 angiomotin isoform X1

UniProtKB/Swiss-Prot

Q8VHG2

Conserved Domains (4) summary

PRK05641
Location:1017 → 1049

PRK05641; putative acetyl-CoA carboxylase biotin carboxyl carrier protein subunit; Validated

PRK07764
Location:1014 → 1098

PRK07764; DNA polymerase III subunits gamma and tau; Validated

pfam02463
Location:418 → 669

SMC_N; RecF/RecN/SMC N terminal domain

pfam12240
Location:578 → 781

Angiomotin_C; Angiomotin C terminal
XM_030251359.2 → XP_030107219.1 angiomotin isoform X2

Conserved Domains (4) summary

COG1196
Location:101 → 398

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

PRK05641
Location:751 → 783

PRK05641; putative acetyl-CoA carboxylase biotin carboxyl carrier protein subunit; Validated

PRK07764
Location:748 → 832

PRK07764; DNA polymerase III subunits gamma and tau; Validated

pfam12240
Location:312 → 515

Angiomotin_C; Angiomotin C terminal
XM_006528867.5 → XP_006528930.1 angiomotin isoform X3

Conserved Domains (4) summary

COG1196
Location:78 → 375

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

PRK05641
Location:728 → 760

PRK05641; putative acetyl-CoA carboxylase biotin carboxyl carrier protein subunit; Validated

PRK07764
Location:725 → 809

PRK07764; DNA polymerase III subunits gamma and tau; Validated

pfam12240
Location:289 → 492

Angiomotin_C; Angiomotin C terminal