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AGO2 argonaute RISC catalytic component 2 [ Homo sapiens (human) ]

Gene ID: 27161, updated on 3-Feb-2020

Summary

Official Symbol
AGO2provided by HGNC
Official Full Name
argonaute RISC catalytic component 2provided by HGNC
Primary source
HGNC:HGNC:3263
See related
Ensembl:ENSG00000123908 MIM:606229
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
PPD; Q10; CASC7; EIF2C2; LINC00980
Summary
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Expression
Ubiquitous expression in skin (RPKM 3.3), ovary (RPKM 3.3) and 25 other tissues See more
Orthologs

Genomic context

See AGO2 in Genome Data Viewer
Location:
8q24.3
Exon count:
22
Annotation release Status Assembly Chr Location
109.20191205 current GRCh38.p13 (GCF_000001405.39) 8 NC_000008.11 (140520156..140642406, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 8 NC_000008.10 (141541264..141645646, complement)

Chromosome 8 - NC_000008.11Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC105375782 Neighboring gene chromatin accessibility complex subunit 1 Neighboring gene MAGE-like protein 2 Neighboring gene protein tyrosine kinase 2 Neighboring gene microRNA 151a Neighboring gene RNA, 5S ribosomal pseudogene 278

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phenotypes

NHGRI GWAS Catalog

Description
Genome-wide association analyses suggest NELL1 influences adverse metabolic response to HCTZ in African Americans.
NHGRI GWA Catalog

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Nef nef The interaction of HIV-1 Nef with AGO2 inhibits miRNA-induced silencing and the slicing activity of AGO2 PubMed
nef HIV-1 Nef interacts with AGO2 and two GW motifs at residues 12-13 and 140-141 in Nef are required for AGO2 binding PubMed
nef HIV-1 Nef co-localizes with AGO2 in multivesicular bodies PubMed
Pr55(Gag) gag Knockdown of AGO2 by shRNA leads to enhance enrichment of HIV-1 Gag in intracellular vacuoles, but decrease viral particle release in Gag expressing cells PubMed
gag Mov10 overexpression results in translational repression of HIV-1 Gag and that AGO2 and/or RISC function is required to induce this translational repression PubMed
gag The efficiency of incorporation of Mov10, A3G, and A3F into viral particles, which contains both HIV-1 Gag and genomic RNA, is much higher than that of the other P-body proteins AGO2, DCP1a, DCP2, and DDX6 PubMed
gag ABCE1, DDX6, and AGO2 proteins are associated with HIV-1 Gag in the intracellular high-molecular-weight assembly intermediates. DDX6 and AGO2 are enriched at early and late sites of Gag assembly at plasma membrane PubMed
gag HIV-1 Gag interacts with and encapsidates AGO2 into virus particles in a miRNA- and P-body-independent manner. AGO2 is required for HIV-1 replication PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • MGC3183, FLJ34853

Gene Ontology Provided by GOA

Function Evidence Code Pubs
RNA 7-methylguanosine cap binding IDA
Inferred from Direct Assay
more info
PubMed 
RNA binding HDA PubMed 
RNA polymerase II complex binding IDA
Inferred from Direct Assay
more info
PubMed 
core promoter binding IMP
Inferred from Mutant Phenotype
more info
PubMed 
double-stranded RNA binding IDA
Inferred from Direct Assay
more info
PubMed 
endoribonuclease activity IDA
Inferred from Direct Assay
more info
PubMed 
endoribonuclease activity TAS
Traceable Author Statement
more info
 
endoribonuclease activity, cleaving miRNA-paired mRNA IDA
Inferred from Direct Assay
more info
PubMed 
endoribonuclease activity, cleaving miRNA-paired mRNA IMP
Inferred from Mutant Phenotype
more info
PubMed 
endoribonuclease activity, cleaving siRNA-paired mRNA IDA
Inferred from Direct Assay
more info
PubMed 
mRNA cap binding IPI
Inferred from Physical Interaction
more info
PubMed 
metal ion binding IEA
Inferred from Electronic Annotation
more info
 
miRNA binding IDA
Inferred from Direct Assay
more info
PubMed 
miRNA binding TAS
Traceable Author Statement
more info
PubMed 
protein C-terminus binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
siRNA binding IDA
Inferred from Direct Assay
more info
PubMed 
contributes_to siRNA binding IDA
Inferred from Direct Assay
more info
PubMed 
single-stranded RNA binding IDA
Inferred from Direct Assay
more info
PubMed 
translation initiation factor activity NAS
Non-traceable Author Statement
more info
PubMed 
Process Evidence Code Pubs
RNA phosphodiester bond hydrolysis, endonucleolytic IEA
Inferred from Electronic Annotation
more info
 
RNA secondary structure unwinding IDA
Inferred from Direct Assay
more info
PubMed 
RNA secondary structure unwinding IMP
Inferred from Mutant Phenotype
more info
PubMed 
Wnt signaling pathway, calcium modulating pathway TAS
Traceable Author Statement
more info
 
gene silencing by RNA ISS
Inferred from Sequence or Structural Similarity
more info
 
mRNA cleavage involved in gene silencing by miRNA IDA
Inferred from Direct Assay
more info
PubMed 
mRNA cleavage involved in gene silencing by miRNA IMP
Inferred from Mutant Phenotype
more info
PubMed 
mRNA cleavage involved in gene silencing by siRNA IDA
Inferred from Direct Assay
more info
PubMed 
mRNA cleavage involved in gene silencing by siRNA IMP
Inferred from Mutant Phenotype
more info
PubMed 
miRNA loading onto RISC involved in gene silencing by miRNA IDA
Inferred from Direct Assay
more info
PubMed 
miRNA mediated inhibition of translation IDA
Inferred from Direct Assay
more info
PubMed 
miRNA mediated inhibition of translation IMP
Inferred from Mutant Phenotype
more info
PubMed 
miRNA metabolic process TAS
Traceable Author Statement
more info
 
negative regulation of amyloid precursor protein biosynthetic process ISS
Inferred from Sequence or Structural Similarity
more info
 
negative regulation of gene expression TAS
Traceable Author Statement
more info
 
negative regulation of translational initiation IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of angiogenesis IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of gene expression TAS
Traceable Author Statement
more info
 
positive regulation of miRNA mediated inhibition of translation IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay ISS
Inferred from Sequence or Structural Similarity
more info
 
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening ISS
Inferred from Sequence or Structural Similarity
more info
 
positive regulation of transcription by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of trophoblast cell migration IMP
Inferred from Mutant Phenotype
more info
PubMed 
post-embryonic development IEA
Inferred from Electronic Annotation
more info
 
posttranscriptional gene silencing by RNA TAS
Traceable Author Statement
more info
 
pre-miRNA processing IDA
Inferred from Direct Assay
more info
PubMed 
production of miRNAs involved in gene silencing by miRNA IDA
Inferred from Direct Assay
more info
PubMed 
production of siRNA involved in RNA interference TAS
Traceable Author Statement
more info
 
regulation of gene silencing by miRNA TAS
Traceable Author Statement
more info
 
siRNA loading onto RISC involved in RNA interference IC
Inferred by Curator
more info
PubMed 
siRNA loading onto RISC involved in RNA interference IDA
Inferred from Direct Assay
more info
PubMed 
translation NAS
Non-traceable Author Statement
more info
PubMed 
translational initiation IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
P-body IDA
Inferred from Direct Assay
more info
PubMed 
RISC complex IC
Inferred by Curator
more info
PubMed 
RISC complex IDA
Inferred from Direct Assay
more info
PubMed 
RISC complex IPI
Inferred from Physical Interaction
more info
PubMed 
RISC-loading complex IDA
Inferred from Direct Assay
more info
PubMed 
cell junction IDA
Inferred from Direct Assay
more info
 
cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
cytosol TAS
Traceable Author Statement
more info
 
dendrite IEA
Inferred from Electronic Annotation
more info
 
extracellular exosome IDA
Inferred from Direct Assay
more info
PubMed 
mRNA cap binding complex IDA
Inferred from Direct Assay
more info
PubMed 
membrane HDA PubMed 
nucleoplasm IDA
Inferred from Direct Assay
more info
 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IC
Inferred by Curator
more info
PubMed 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 
polysome IDA
Inferred from Direct Assay
more info
PubMed 
ribonucleoprotein complex IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
protein argonaute-2
Names
PAZ Piwi domain protein
argonaute 2, RISC catalytic component
cancer susceptibility candidate 7
cancer susceptibility candidate 7 (non-protein coding)
eukaryotic translation initiation factor 2C, 2
long intergenic non-protein coding RNA 980
protein slicer
NP_001158095.1
NP_036286.2
XP_011515267.1
XP_011515268.1
XP_011515270.2
XP_016868806.1

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001164623.3NP_001158095.1  protein argonaute-2 isoform 2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1.
    Source sequence(s)
    AC067931, AC107375
    Consensus CDS
    CCDS55279.1
    UniProtKB/Swiss-Prot
    Q9UKV8
    Related
    ENSP00000430176.1, ENST00000519980.5
    Conserved Domains (4) summary
    cd02846
    Location:227347
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:392783
    Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...
    pfam08699
    Location:176226
    ArgoL1; Argonaute linker 1 domain
    pfam16486
    Location:92166
    ArgoN; N-terminal domain of argonaute
  2. NM_012154.5NP_036286.2  protein argonaute-2 isoform 1

    See identical proteins and their annotated locations for NP_036286.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AC067931, AC107375
    Consensus CDS
    CCDS6380.1
    UniProtKB/Swiss-Prot
    Q9UKV8
    Related
    ENSP00000220592.5, ENST00000220592.10
    Conserved Domains (4) summary
    cd02846
    Location:227347
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:392817
    Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...
    pfam08699
    Location:176226
    ArgoL1; Argonaute linker 1 domain
    pfam16486
    Location:92166
    ArgoN; N-terminal domain of argonaute

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.20191205

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000008.11 Reference GRCh38.p13 Primary Assembly

    Range
    140520156..140642406 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011516968.2XP_011515270.2  protein argonaute-2 isoform X1

  2. XM_017013317.2XP_016868806.1  protein argonaute-2 isoform X4

    Conserved Domains (4) summary
    cd02846
    Location:150270
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:315740
    Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...
    pfam08699
    Location:100149
    ArgoL1; Argonaute linker 1 domain
    pfam16486
    Location:289
    ArgoN; N-terminal domain of argonaute
  3. XM_011516966.3XP_011515268.1  protein argonaute-2 isoform X3

    See identical proteins and their annotated locations for XP_011515268.1

    Conserved Domains (4) summary
    cd02846
    Location:181301
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:346771
    Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
    pfam08699
    Location:132180
    ArgoL1; Argonaute linker 1 domain
    pfam16486
    Location:46120
    ArgoN; N-terminal domain of argonaute
  4. XM_011516965.2XP_011515267.1  protein argonaute-2 isoform X2

    Conserved Domains (5) summary
    PLN03202
    Location:39872
    PLN03202; protein argonaute; Provisional
    cd02846
    Location:241361
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:406831
    Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...
    pfam08699
    Location:191240
    ArgoL1; Argonaute linker 1 domain
    pfam16486
    Location:42180
    ArgoN; N-terminal domain of argonaute
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