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AGO2 argonaute RISC catalytic component 2 [ Homo sapiens (human) ]

Gene ID: 27161, updated on 5-Feb-2023

Summary

Official Symbol
AGO2provided by HGNC
Official Full Name
argonaute RISC catalytic component 2provided by HGNC
Primary source
HGNC:HGNC:3263
See related
Ensembl:ENSG00000123908 MIM:606229; AllianceGenome:HGNC:3263
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
PPD; Q10; CASC7; EIF2C2; LESKRES; LINC00980
Summary
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Expression
Ubiquitous expression in skin (RPKM 3.3), ovary (RPKM 3.3) and 25 other tissues See more
Orthologs
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Genomic context

See AGO2 in Genome Data Viewer
Location:
8q24.3
Exon count:
23
Annotation release Status Assembly Chr Location
110 current GRCh38.p14 (GCF_000001405.40) 8 NC_000008.11 (140520156..140642313, complement)
110 current T2T-CHM13v2.0 (GCF_009914755.1) 8 NC_060932.1 (141640512..141755648, complement)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 8 NC_000008.10 (141530255..141645732, complement)

Chromosome 8 - NC_000008.11Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC105375782 Neighboring gene chromatin accessibility complex subunit 1 Neighboring gene MAGE-like protein 2 Neighboring gene U2 spliceosomal RNA Neighboring gene E2F1-regulated inhibitor of cell death Neighboring gene protein tyrosine kinase 2 Neighboring gene microRNA 151a

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phenotypes

Associated conditions

Description Tests
Lessel-Kreienkamp syndrome
MedGen: C5436892 OMIM: 619149 GeneReviews: Not available
not available

EBI GWAS Catalog

Description
Genome-wide association analyses suggest NELL1 influences adverse metabolic response to HCTZ in African Americans.
EBI GWAS Catalog

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Nef nef The interaction of HIV-1 Nef with AGO2 inhibits miRNA-induced silencing and the slicing activity of AGO2 PubMed
nef HIV-1 Nef interacts with AGO2 and two GW motifs at residues 12-13 and 140-141 in Nef are required for AGO2 binding PubMed
nef HIV-1 Nef co-localizes with AGO2 in multivesicular bodies PubMed
Pr55(Gag) gag Knockdown of AGO2 by shRNA leads to enhance enrichment of HIV-1 Gag in intracellular vacuoles, but decrease viral particle release in Gag expressing cells PubMed
gag Mov10 overexpression results in translational repression of HIV-1 Gag and that AGO2 and/or RISC function is required to induce this translational repression PubMed
gag The efficiency of incorporation of Mov10, A3G, and A3F into viral particles, which contains both HIV-1 Gag and genomic RNA, is much higher than that of the other P-body proteins AGO2, DCP1a, DCP2, and DDX6 PubMed
gag ABCE1, DDX6, and AGO2 proteins are associated with HIV-1 Gag in the intracellular high-molecular-weight assembly intermediates. DDX6 and AGO2 are enriched at early and late sites of Gag assembly at plasma membrane PubMed
gag HIV-1 Gag interacts with and encapsidates AGO2 into virus particles in a miRNA- and P-body-independent manner. AGO2 is required for HIV-1 replication PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • MGC3183, FLJ34853

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables RNA 7-methylguanosine cap binding IDA
Inferred from Direct Assay
more info
PubMed 
enables RNA binding HDA PubMed 
enables RNA endonuclease activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
enables RNA endonuclease activity IDA
Inferred from Direct Assay
more info
PubMed 
enables RNA endonuclease activity TAS
Traceable Author Statement
more info
 
enables RNA polymerase II complex binding IDA
Inferred from Direct Assay
more info
PubMed 
enables core promoter sequence-specific DNA binding IMP
Inferred from Mutant Phenotype
more info
PubMed 
enables double-stranded RNA binding IDA
Inferred from Direct Assay
more info
PubMed 
enables endoribonuclease activity, cleaving miRNA-paired mRNA IDA
Inferred from Direct Assay
more info
PubMed 
enables endoribonuclease activity, cleaving miRNA-paired mRNA IMP
Inferred from Mutant Phenotype
more info
PubMed 
enables endoribonuclease activity, cleaving siRNA-paired mRNA IDA
Inferred from Direct Assay
more info
PubMed 
enables mRNA cap binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
 
enables miRNA binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
enables miRNA binding IDA
Inferred from Direct Assay
more info
PubMed 
enables miRNA binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables miRNA binding TAS
Traceable Author Statement
more info
PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
contributes_to siRNA binding IDA
Inferred from Direct Assay
more info
PubMed 
enables siRNA binding IDA
Inferred from Direct Assay
more info
PubMed 
enables single-stranded RNA binding IDA
Inferred from Direct Assay
more info
PubMed 
enables translation initiation factor activity NAS
Non-traceable Author Statement
more info
PubMed 
Process Evidence Code Pubs
involved_in RISC complex assembly IDA
Inferred from Direct Assay
more info
PubMed 
involved_in RNA phosphodiester bond hydrolysis, endonucleolytic IEA
Inferred from Electronic Annotation
more info
 
involved_in RNA secondary structure unwinding IDA
Inferred from Direct Assay
more info
PubMed 
involved_in RNA secondary structure unwinding IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in RNA-mediated gene silencing ISS
Inferred from Sequence or Structural Similarity
more info
 
involved_in RNA-mediated post-transcriptional gene silencing TAS
Traceable Author Statement
more info
 
involved_in miRNA metabolic process IEA
Inferred from Electronic Annotation
more info
 
involved_in miRNA processing IDA
Inferred from Direct Assay
more info
PubMed 
involved_in miRNA-mediated gene silencing by inhibition of translation IDA
Inferred from Direct Assay
more info
PubMed 
involved_in miRNA-mediated gene silencing by inhibition of translation IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in miRNA-mediated gene silencing by mRNA destabilization IDA
Inferred from Direct Assay
more info
PubMed 
involved_in miRNA-mediated gene silencing by mRNA destabilization IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in negative regulation of amyloid precursor protein biosynthetic process ISS
Inferred from Sequence or Structural Similarity
more info
 
involved_in negative regulation of translational initiation IDA
Inferred from Direct Assay
more info
PubMed 
involved_in positive regulation of angiogenesis IDA
Inferred from Direct Assay
more info
PubMed 
involved_in positive regulation of gene expression IEA
Inferred from Electronic Annotation
more info
 
involved_in positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay ISS
Inferred from Sequence or Structural Similarity
more info
 
involved_in positive regulation of nuclear-transcribed mRNA poly(A) tail shortening ISS
Inferred from Sequence or Structural Similarity
more info
 
involved_in positive regulation of transcription by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in positive regulation of trophoblast cell migration IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in post-embryonic development IEA
Inferred from Electronic Annotation
more info
 
involved_in pre-miRNA processing IDA
Inferred from Direct Assay
more info
PubMed 
involved_in siRNA processing IDA
Inferred from Direct Assay
more info
PubMed 
involved_in siRNA-mediated gene silencing by mRNA destabilization IDA
Inferred from Direct Assay
more info
PubMed 
involved_in siRNA-mediated gene silencing by mRNA destabilization IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in translation NAS
Non-traceable Author Statement
more info
PubMed 
involved_in translational initiation IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
located_in P-body IDA
Inferred from Direct Assay
more info
PubMed 
part_of RISC complex IDA
Inferred from Direct Assay
more info
PubMed 
part_of RISC complex IGI
Inferred from Genetic Interaction
more info
PubMed 
part_of RISC complex IPI
Inferred from Physical Interaction
more info
PubMed 
part_of RISC-loading complex IDA
Inferred from Direct Assay
more info
PubMed 
part_of RISC-loading complex IPI
Inferred from Physical Interaction
more info
PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
located_in cytoplasmic ribonucleoprotein granule IDA
Inferred from Direct Assay
more info
 
located_in cytosol IDA
Inferred from Direct Assay
more info
 
located_in cytosol TAS
Traceable Author Statement
more info
 
located_in dendrite IEA
Inferred from Electronic Annotation
more info
 
located_in extracellular exosome IDA
Inferred from Direct Assay
more info
PubMed 
part_of mRNA cap binding complex IDA
Inferred from Direct Assay
more info
PubMed 
located_in membrane HDA PubMed 
located_in nucleoplasm TAS
Traceable Author Statement
more info
 
located_in nucleus IC
Inferred by Curator
more info
PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
PubMed 
part_of polysome IDA
Inferred from Direct Assay
more info
PubMed 
part_of ribonucleoprotein complex IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
protein argonaute-2
Names
PAZ Piwi domain protein
argonaute 2, RISC catalytic component
cancer susceptibility candidate 7 (non-protein coding)
eukaryotic translation initiation factor 2C, 2
long intergenic non-protein coding RNA 980
protein slicer
NP_001158095.1
NP_036286.2
XP_011515267.1
XP_011515270.3
XP_047277651.1
XP_047277652.1
XP_047277653.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001164623.3NP_001158095.1  protein argonaute-2 isoform 2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1.
    Source sequence(s)
    AC067931, AC107375
    Consensus CDS
    CCDS55279.1
    UniProtKB/Swiss-Prot
    Q9UKV8
    Related
    ENSP00000430176.1, ENST00000519980.5
    Conserved Domains (4) summary
    cd02846
    Location:227347
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:392783
    Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...
    pfam08699
    Location:176226
    ArgoL1; Argonaute linker 1 domain
    pfam16486
    Location:92166
    ArgoN; N-terminal domain of argonaute
  2. NM_012154.5NP_036286.2  protein argonaute-2 isoform 1

    See identical proteins and their annotated locations for NP_036286.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AC067931, AC107375
    Consensus CDS
    CCDS6380.1
    UniProtKB/Swiss-Prot
    Q96ID1, Q9UKV8
    Related
    ENSP00000220592.5, ENST00000220592.10
    Conserved Domains (4) summary
    cd02846
    Location:227347
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:392817
    Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
    pfam08699
    Location:176226
    ArgoL1; Argonaute linker 1 domain
    pfam16486
    Location:92166
    ArgoN; N-terminal domain of argonaute

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 110 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000008.11 Reference GRCh38.p14 Primary Assembly

    Range
    140520156..140642313 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011516968.3XP_011515270.3  protein argonaute-2 isoform X3

  2. XM_047421697.1XP_047277653.1  protein argonaute-2 isoform X3

  3. XM_047421695.1XP_047277651.1  protein argonaute-2 isoform X2

  4. XM_011516965.3XP_011515267.1  protein argonaute-2 isoform X1

    Conserved Domains (5) summary
    PLN03202
    Location:39872
    PLN03202; protein argonaute; Provisional
    cd02846
    Location:241361
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:406831
    Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...
    pfam08699
    Location:191240
    ArgoL1; Argonaute linker 1 domain
    pfam16486
    Location:42180
    ArgoN; N-terminal domain of argonaute
  5. XM_047421696.1XP_047277652.1  protein argonaute-2 isoform X3

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060932.1 Alternate T2T-CHM13v2.0

    Range
    141640512..141755648 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)