U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Spag1 sperm associated antigen 1 [ Mus musculus (house mouse) ]

Gene ID: 26942, updated on 25-Jan-2023

Summary

Official Symbol
Spag1provided by MGI
Official Full Name
sperm associated antigen 1provided by MGI
Primary source
MGI:MGI:1349387
See related
Ensembl:ENSMUSG00000037617 AllianceGenome:MGI:1349387
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
tpis
Summary
Predicted to enable GTP binding activity and hydrolase activity. Predicted to be involved in axonemal dynein complex assembly. Predicted to act upstream of or within single fertilization. Predicted to be located in cytoplasm. Predicted to be active in cytosol. Human ortholog(s) of this gene implicated in primary ciliary dyskinesia 28. Orthologous to human SPAG1 (sperm associated antigen 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in cerebellum adult (RPKM 3.6), testis adult (RPKM 2.9) and 22 other tissues See more
Orthologs
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

See Spag1 in Genome Data Viewer
Location:
15; 15 B3.1
Exon count:
25
Annotation release Status Assembly Chr Location
109 current GRCm39 (GCF_000001635.27) 15 NC_000081.7 (36175629..36235767)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 15 NC_000081.6 (36176229..36235621)

Chromosome 15 - NC_000081.7Genomic Context describing neighboring genes Neighboring gene regulator of G-protein signalling 22 Neighboring gene heterogeneous nuclear ribonucleoprotein C pseudogene Neighboring gene F-box protein 43 Neighboring gene predicted gene, 22979 Neighboring gene polymerase (RNA) II (DNA directed) polypeptide K Neighboring gene predicted gene, 23754 Neighboring gene predicted gene, 41293 Neighboring gene microRNA 8097 Neighboring gene ring finger protein 19A Neighboring gene lactate dehydrogenase A pseudogene

Genomic regions, transcripts, and products

Expression

  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

Variation

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (2) 

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables GTP binding IEA
Inferred from Electronic Annotation
more info
 
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
 
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
 
Process Evidence Code Pubs
involved_in axonemal dynein complex assembly IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
acts_upstream_of_or_within single fertilization IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
part_of chaperone complex ISO
Inferred from Sequence Orthology
more info
 
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
located_in cytosol ISO
Inferred from Sequence Orthology
more info
 

General protein information

Preferred Names
sperm-associated antigen 1
Names
TPR-containing protein involved in spermatogenesis
infertility-related sperm protein Spag-1

NCBI Reference Sequences (RefSeq)

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001359980.1NP_001346909.1  sperm-associated antigen 1 isoform 3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (4) has a distinct N-terminus and is shorter than isoform 1.
    Source sequence(s)
    AC121577
    Consensus CDS
    CCDS37061.1
    UniProtKB/Swiss-Prot
    Q9QZJ4
    Related
    ENSMUSP00000047335.4, ENSMUST00000047348.11
    Conserved Domains (4) summary
    PLN03088
    Location:619795
    PLN03088; SGT1, suppressor of G2 allele of SKP1; Provisional
    TIGR00990
    Location:202686
    3a0801s09; mitochondrial precursor proteins import receptor (72 kDa mitochondrial outermembrane protein) (mitochondrial import receptor for the ADP/ATP carrier) (translocase of outermembrane tom70)
    sd00006
    Location:217241
    TPR; TPR repeat [structural motif]
    pfam13877
    Location:777866
    RPAP3_C; Potential Monad-binding region of RPAP3
  2. NM_001359981.1NP_001346910.1  sperm-associated antigen 1 isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1, 5 and 6 encode the same protein.
    Source sequence(s)
    AC121577
    Conserved Domains (4) summary
    PLN03088
    Location:631807
    PLN03088; SGT1, suppressor of G2 allele of SKP1; Provisional
    TIGR00990
    Location:214698
    3a0801s09; mitochondrial precursor proteins import receptor (72 kDa mitochondrial outermembrane protein) (mitochondrial import receptor for the ADP/ATP carrier) (translocase of outermembrane tom70)
    sd00006
    Location:229253
    TPR; TPR repeat [structural motif]
    pfam13877
    Location:789878
    RPAP3_C; Potential Monad-binding region of RPAP3
  3. NM_001359982.1NP_001346911.1  sperm-associated antigen 1 isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. Variants 1, 5 and 6 encode the same protein.
    Source sequence(s)
    AC121577
    Conserved Domains (4) summary
    PLN03088
    Location:631807
    PLN03088; SGT1, suppressor of G2 allele of SKP1; Provisional
    TIGR00990
    Location:214698
    3a0801s09; mitochondrial precursor proteins import receptor (72 kDa mitochondrial outermembrane protein) (mitochondrial import receptor for the ADP/ATP carrier) (translocase of outermembrane tom70)
    sd00006
    Location:229253
    TPR; TPR repeat [structural motif]
    pfam13877
    Location:789878
    RPAP3_C; Potential Monad-binding region of RPAP3
  4. NM_001359983.1NP_001346912.1  sperm-associated antigen 1 isoform 2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.
    Source sequence(s)
    AC121577
    Conserved Domains (4) summary
    PLN03088
    Location:3106
    PLN03088; SGT1, suppressor of G2 allele of SKP1; Provisional
    TIGR00990
    Location:200437
    3a0801s09; mitochondrial precursor proteins import receptor (72 kDa mitochondrial outermembrane protein) (mitochondrial import receptor for the ADP/ATP carrier) (translocase of outermembrane tom70)
    sd00006
    Location:374401
    TPR; TPR repeat [structural motif]
    pfam13877
    Location:544633
    RPAP3_C; Potential Monad-binding region of RPAP3
  5. NM_001359984.1NP_001346913.1  sperm-associated antigen 1 isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (6) differs in the 5' UTR compared to variant 1. Variants 1, 5 and 6 encode the same protein.
    Source sequence(s)
    AC121577
    Conserved Domains (4) summary
    PLN03088
    Location:631807
    PLN03088; SGT1, suppressor of G2 allele of SKP1; Provisional
    TIGR00990
    Location:214698
    3a0801s09; mitochondrial precursor proteins import receptor (72 kDa mitochondrial outermembrane protein) (mitochondrial import receptor for the ADP/ATP carrier) (translocase of outermembrane tom70)
    sd00006
    Location:229253
    TPR; TPR repeat [structural motif]
    pfam13877
    Location:789878
    RPAP3_C; Potential Monad-binding region of RPAP3
  6. NM_001416284.1NP_001403213.1  sperm-associated antigen 1 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC121577
  7. NM_012031.4NP_036161.2  sperm-associated antigen 1 isoform 3

    See identical proteins and their annotated locations for NP_036161.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1.
    Source sequence(s)
    AI843673, BB706348, BC046313
    Consensus CDS
    CCDS37061.1
    UniProtKB/Swiss-Prot
    Q80ZX8, Q9QZJ4
    Related
    ENSMUSP00000132233.2, ENSMUST00000171205.3
    Conserved Domains (4) summary
    PLN03088
    Location:619795
    PLN03088; SGT1, suppressor of G2 allele of SKP1; Provisional
    TIGR00990
    Location:202686
    3a0801s09; mitochondrial precursor proteins import receptor (72 kDa mitochondrial outermembrane protein) (mitochondrial import receptor for the ADP/ATP carrier) (translocase of outermembrane tom70)
    sd00006
    Location:217241
    TPR; TPR repeat [structural motif]
    pfam13877
    Location:777866
    RPAP3_C; Potential Monad-binding region of RPAP3

RNA

  1. NR_153381.1 RNA Sequence

    Status: VALIDATED

    Description
    Transcript Variant: This variant (7) lacks an alternate internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    AC121577

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000081.7 Reference GRCm39 C57BL/6J

    Range
    36175629..36235767
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036159439.1XP_036015332.1  sperm-associated antigen 1 isoform X2

    UniProtKB/Swiss-Prot
    Q9QZJ4
    Conserved Domains (4) summary
    PLN03088
    Location:619795
    PLN03088; SGT1, suppressor of G2 allele of SKP1; Provisional
    TIGR00990
    Location:202686
    3a0801s09; mitochondrial precursor proteins import receptor (72 kDa mitochondrial outermembrane protein) (mitochondrial import receptor for the ADP/ATP carrier) (translocase of outermembrane tom70)
    sd00006
    Location:217241
    TPR; TPR repeat [structural motif]
    pfam13877
    Location:777866
    RPAP3_C; Potential Monad-binding region of RPAP3
  2. XM_036159438.1XP_036015331.1  sperm-associated antigen 1 isoform X1

    Conserved Domains (4) summary
    PLN03088
    Location:631807
    PLN03088; SGT1, suppressor of G2 allele of SKP1; Provisional
    TIGR00990
    Location:214698
    3a0801s09; mitochondrial precursor proteins import receptor (72 kDa mitochondrial outermembrane protein) (mitochondrial import receptor for the ADP/ATP carrier) (translocase of outermembrane tom70)
    sd00006
    Location:229253
    TPR; TPR repeat [structural motif]
    pfam13877
    Location:789878
    RPAP3_C; Potential Monad-binding region of RPAP3
  3. XM_017316634.3XP_017172123.1  sperm-associated antigen 1 isoform X1

    Conserved Domains (4) summary
    PLN03088
    Location:631807
    PLN03088; SGT1, suppressor of G2 allele of SKP1; Provisional
    TIGR00990
    Location:214698
    3a0801s09; mitochondrial precursor proteins import receptor (72 kDa mitochondrial outermembrane protein) (mitochondrial import receptor for the ADP/ATP carrier) (translocase of outermembrane tom70)
    sd00006
    Location:229253
    TPR; TPR repeat [structural motif]
    pfam13877
    Location:789878
    RPAP3_C; Potential Monad-binding region of RPAP3
  4. XM_036159441.1XP_036015334.1  sperm-associated antigen 1 isoform X3

    Conserved Domains (4) summary
    PLN03088
    Location:3106
    PLN03088; SGT1, suppressor of G2 allele of SKP1; Provisional
    TIGR00990
    Location:200437
    3a0801s09; mitochondrial precursor proteins import receptor (72 kDa mitochondrial outermembrane protein) (mitochondrial import receptor for the ADP/ATP carrier) (translocase of outermembrane tom70)
    sd00006
    Location:374401
    TPR; TPR repeat [structural motif]
    pfam13877
    Location:544633
    RPAP3_C; Potential Monad-binding region of RPAP3
  5. XM_006520076.3XP_006520139.1  sperm-associated antigen 1 isoform X2

    See identical proteins and their annotated locations for XP_006520139.1

    UniProtKB/Swiss-Prot
    Q80ZX8, Q9QZJ4
    Conserved Domains (4) summary
    PLN03088
    Location:619795
    PLN03088; SGT1, suppressor of G2 allele of SKP1; Provisional
    TIGR00990
    Location:202686
    3a0801s09; mitochondrial precursor proteins import receptor (72 kDa mitochondrial outermembrane protein) (mitochondrial import receptor for the ADP/ATP carrier) (translocase of outermembrane tom70)
    sd00006
    Location:217241
    TPR; TPR repeat [structural motif]
    pfam13877
    Location:777866
    RPAP3_C; Potential Monad-binding region of RPAP3
  6. XM_006520078.5XP_006520141.1  sperm-associated antigen 1 isoform X3

    See identical proteins and their annotated locations for XP_006520141.1

    UniProtKB/Swiss-Prot
    Q80ZX8
    Conserved Domains (4) summary
    PLN03088
    Location:3106
    PLN03088; SGT1, suppressor of G2 allele of SKP1; Provisional
    TIGR00990
    Location:200437
    3a0801s09; mitochondrial precursor proteins import receptor (72 kDa mitochondrial outermembrane protein) (mitochondrial import receptor for the ADP/ATP carrier) (translocase of outermembrane tom70)
    sd00006
    Location:374401
    TPR; TPR repeat [structural motif]
    pfam13877
    Location:544633
    RPAP3_C; Potential Monad-binding region of RPAP3

RNA

  1. XR_001781540.3 RNA Sequence