Hspa4 heat shock protein family A (Hsp70) member 4 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Hspa4provided by RGD
Official Full Name: heat shock protein family A (Hsp70) member 4provided by RGD
Primary source: RGD:628878
See related: Ensembl:ENSRNOG00000016596 AllianceGenome:RGD:628878
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Hsp70; irp94; Hsp110
Summary: Enables RNA polymerase II-specific DNA-binding transcription factor binding activity. Involved in several processes, including negative regulation of macromolecule metabolic process; positive regulation of angiogenesis; and regulation of microglial cell activation. Located in lipid droplet. Used to study adult respiratory distress syndrome and middle cerebral artery infarction. Biomarker of Parkinson's disease and liver benign neoplasm. Human ortholog(s) of this gene implicated in Chagas disease. Orthologous to human HSPA4 (heat shock protein family A (Hsp70) member 4). [provided by Alliance of Genome Resources, Jul 2025]
Expression: Biased expression in Kidney (RPKM 486.9), Adrenal (RPKM 469.5) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 10q22

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (37908866..37951994, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (37408025..37449080, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (38601624..38642397, complement)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dynamically changed HSP70 after reperfusion following cerebral infarction in human and rats: correlation with p38 MAPK.
Title: Dynamically changed HSP70 after reperfusion following cerebral infarction in human and rats: correlation with p38 MAPK.
Heat shock protein 70 protects the lungs from hyperoxic injury in a neonatal rat model of bronchopulmonary dysplasia.
Title: Heat shock protein 70 protects the lungs from hyperoxic injury in a neonatal rat model of bronchopulmonary dysplasia.
Overexpression of miR-144-3p alleviates polycystic ovaries syndrome through targeting expression of HSP-70.
Title: Overexpression of miR-144-3p alleviates polycystic ovaries syndrome through targeting expression of HSP-70.
Heat-Stress Preconditioning Attenuates Behavioral Responses to Psychological Stress: The Role of HSP-70 in Modulating Stress Responses.
Title: Heat-Stress Preconditioning Attenuates Behavioral Responses to Psychological Stress: The Role of HSP-70 in Modulating Stress Responses.
Male rats were trained in contextual fear conditioning task and HSP70 content was analyzed by western blot in the hippocampus at different time points. We observed rapid and transient elevations in HSP70 60min following training. Double immunofluorescence with GFAP and HSP72 revealed that astrocytes were not the site for HSP72 induction by CFC training.
Title: HSP70 Facilitates Memory Consolidation of Fear Conditioning through MAPK Pathway in the Hippocampus.
Data suggest that heat stress enhances ovarian levels of Hsp70 and affects Erk1/2 and Atf3 phosphorylation levels, but not c-Akt activation/phosphorylation, in pseudo-pregnant rats during luteal regression induced by cloprostenol, a prostaglandin F2alpha analog. (Hsp70 = heat shock 70 kDa protein; Erk = extracellular signal-regulated kinase; Atf3 = activating transcription factor-3; c-Akt = proto-oncogene protein c-Akt)
Title: Temporal regulation of extracellular signal-regulated kinase 1/2 phosphorylation, heat shock protein 70 and activating transcription factor 3 during prostaglandin F-induced luteal regression in pseudopregnant rats following heat stress.
The results showed that regular Hot-water immersion (HWI) resulted in lower HSP-70 expression than single HWI did in the organs, indicating that regular HWI caused less heat stress than single HWI.
Title: Heat adaptation from regular hot water immersion decreases proinflammatory responses, HSP70 expression, and physical heat stress.
Data suggest that vitamin A, when used as an unnecessary dietary supplement, decreases skeletal muscle expression of anti-inflammatory mediators interleukin-10 and heat shock protein 70, important factors for positive exercise adaptations and tissue damage prevention.
Title: Vitamin A Oral Supplementation Induces Oxidative Stress and Suppresses IL-10 and HSP70 in Skeletal Muscle of Trained Rats.
increased expression/activity of SLK activates the HSF1-Hsp70 pathway.
Title: Ste20-like kinase, SLK, activates the heat shock factor 1 - Hsp70 pathway.
Renal Hsp70 expression is decreased in spontaneously hypertensive rats before the development of hypertension.
Title: Changes in renal WT-1 expression preceding hypertension development.

Submit: New GeneRIF Correction See all GeneRIFs (103)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH65703 (e-PCR)
- UniSTS:30291

AW535242 (e-PCR)
- UniSTS:260726

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent protein folding chaperone	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables adenyl-nucleotide exchange factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in chaperone-mediated protein complex assembly	ISO Inferred from Sequence Orthology more info
involved_in kidney development	IEP Inferred from Expression Pattern more info	PubMed
involved_in negative regulation of apoptotic process	IEP Inferred from Expression Pattern more info	PubMed
involved_in negative regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of angiogenesis	IEP Inferred from Expression Pattern more info	PubMed
involved_in protein folding	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein import into mitochondrial matrix	ISO Inferred from Sequence Orthology more info
involved_in regulation of microglial cell activation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in extracellular exosome	ISO Inferred from Sequence Orthology more info
located_in lipid droplet	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in organelle	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: heat shock 70 kDa protein 4

Names: heat shock protein 4; heat shock protein family A member 4; ischemia responsive 94 kDa protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_153629.1 → NP_705893.1 heat shock 70 kDa protein 4

See identical proteins and their annotated locations for NP_705893.1

Status: PROVISIONAL

Source sequence(s)

AF077354

UniProtKB/Swiss-Prot

O88600

UniProtKB/TrEMBL

F1LRV4

Related

ENSRNOP00000023628.3, ENSRNOT00000023628.5

Conserved Domains (2) summary

PTZ00121
Location:499 → 743

PTZ00121; MAEBL; Provisional

cd11737
Location:2 → 384

HSPA4_NBD; Nucleotide-binding domain of HSPA4

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086028.1 Reference GRCr8

Range

37908866..37951994 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039085314.2 → XP_038941242.1 heat shock 70 kDa protein 4 isoform X1

UniProtKB/TrEMBL

F1LRV4

Conserved Domains (2) summary

cd11737
Location:2 → 384

HSPA4_NBD; Nucleotide-binding domain of HSPA4

TIGR00927
Location:502 → 636

2A1904; K+-dependent Na+/Ca+ exchanger