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AGO1 argonaute RISC component 1 [ Homo sapiens (human) ]

Gene ID: 26523, updated on 1-Jun-2020

Summary

Official Symbol
AGO1provided by HGNC
Official Full Name
argonaute RISC component 1provided by HGNC
Primary source
HGNC:HGNC:3262
See related
Ensembl:ENSG00000092847 MIM:606228
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
Q99; EIF2C; hAgo1; EIF2C1; GERP95
Summary
This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015]
Expression
Ubiquitous expression in skin (RPKM 4.5), brain (RPKM 3.9) and 25 other tissues See more
Orthologs

Genomic context

See AGO1 in Genome Data Viewer
Location:
1p34.3
Exon count:
20
Annotation release Status Assembly Chr Location
109.20200522 current GRCh38.p13 (GCF_000001405.39) 1 NC_000001.11 (35869808..35930532)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (36335412..36395211)

Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene claspin Neighboring gene argonaute RISC component 4 Neighboring gene argonaute RISC catalytic component 3 Neighboring gene uncharacterized LOC105378647 Neighboring gene magnesium transporter 1 pseudogene

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Pr55(Gag) gag HIV-1 Gag co-immunoprecipitates with endogenous AGO1 in 293T cells PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • DKFZp686M13167

Gene Ontology Provided by GOA

Function Evidence Code Pubs
RNA binding HDA PubMed 
RNA binding IDA
Inferred from Direct Assay
more info
PubMed 
RNA polymerase II complex binding IDA
Inferred from Direct Assay
more info
PubMed 
RNA polymerase II proximal promoter sequence-specific DNA binding IEA
Inferred from Electronic Annotation
more info
 
core promoter sequence-specific DNA binding IMP
Inferred from Mutant Phenotype
more info
PubMed 
double-stranded RNA binding IDA
Inferred from Direct Assay
more info
PubMed 
NOT endoribonuclease activity IDA
Inferred from Direct Assay
more info
PubMed 
miRNA binding IDA
Inferred from Direct Assay
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
single-stranded RNA binding IDA
Inferred from Direct Assay
more info
PubMed 
Process Evidence Code Pubs
RNA phosphodiester bond hydrolysis, endonucleolytic IEA
Inferred from Electronic Annotation
more info
 
RNA secondary structure unwinding IDA
Inferred from Direct Assay
more info
PubMed 
RNA secondary structure unwinding IMP
Inferred from Mutant Phenotype
more info
PubMed 
Wnt signaling pathway, calcium modulating pathway TAS
Traceable Author Statement
more info
 
NOT mRNA cleavage involved in gene silencing by miRNA IDA
Inferred from Direct Assay
more info
PubMed 
NOT mRNA cleavage involved in gene silencing by siRNA IDA
Inferred from Direct Assay
more info
PubMed 
miRNA loading onto RISC involved in gene silencing by miRNA IDA
Inferred from Direct Assay
more info
PubMed 
miRNA mediated inhibition of translation IDA
Inferred from Direct Assay
more info
PubMed 
miRNA metabolic process IEA
Inferred from Electronic Annotation
more info
 
negative regulation of angiogenesis IMP
Inferred from Mutant Phenotype
more info
PubMed 
negative regulation of gene expression TAS
Traceable Author Statement
more info
 
nuclear-transcribed mRNA catabolic process IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of NIK/NF-kappaB signaling IEA
Inferred from Electronic Annotation
more info
 
positive regulation of gene expression TAS
Traceable Author Statement
more info
 
positive regulation of transcription by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
PubMed 
posttranscriptional gene silencing by RNA TAS
Traceable Author Statement
more info
 
pre-miRNA processing IDA
Inferred from Direct Assay
more info
PubMed 
production of miRNAs involved in gene silencing by miRNA IMP
Inferred from Mutant Phenotype
more info
PubMed 
regulation of gene silencing by miRNA TAS
Traceable Author Statement
more info
 
regulation of megakaryocyte differentiation TAS
Traceable Author Statement
more info
 
Component Evidence Code Pubs
P-body IEA
Inferred from Electronic Annotation
more info
 
RISC complex IDA
Inferred from Direct Assay
more info
PubMed 
RISC-loading complex IDA
Inferred from Direct Assay
more info
PubMed 
cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
cytosol TAS
Traceable Author Statement
more info
 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IC
Inferred by Curator
more info
PubMed 
polysome IDA
Inferred from Direct Assay
more info
PubMed 
ribonucleoprotein complex IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
protein argonaute-1
Names
Golgi Endoplasmic Reticulum protein 95 kDa
argonaute 1, RISC catalytic component
argonaute RISC catalytic component 1
argonaute1
eIF-2C 1
eIF2C 1
eukaryotic translation initiation factor 2C, 1
putative RNA-binding protein Q99

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001317122.1NP_001304051.1  protein argonaute-1 isoform 1x

    Status: REVIEWED

    Description
    Transcript Variant: Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the longer, C-terminally extended isoform (1x). As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'.
    Source sequence(s)
    AF093097, AK314148, BC063275, CR936823, HY052874
    UniProtKB/Swiss-Prot
    Q9UL18
    UniProtKB/TrEMBL
    B2RAD8
    Related
    ENSP00000501450.1, ENST00000674304.1
    Conserved Domains (5) summary
    PLN03202
    Location:26856
    PLN03202; protein argonaute; Provisional
    cd02846
    Location:225345
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:390815
    Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...
    pfam08699
    Location:175224
    ArgoL1; Argonaute linker 1 domain
    pfam16486
    Location:26164
    ArgoN; N-terminal domain of argonaute
  2. NM_001317123.1NP_001304052.1  protein argonaute-1 isoform 2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) contains an alternate 5' terminal exon, which results in translation initiation from an in-frame, downstream start codon compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1.
    Source sequence(s)
    AF093097, AK001622, AK314148, CR936823
    Consensus CDS
    CCDS81300.1
    UniProtKB/Swiss-Prot
    Q9UL18
    UniProtKB/TrEMBL
    B2RAD8, B3KME0, Q5TA58
    Related
    ENSP00000362302.1, ENST00000373206.5
    Conserved Domains (4) summary
    cd02846
    Location:150270
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:315740
    Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
    pfam08699
    Location:101149
    ArgoL1; Argonaute linker 1 domain
    pfam16486
    Location:1589
    ArgoN; N-terminal domain of argonaute
  3. NM_012199.5NP_036331.1  protein argonaute-1 isoform 1

    See identical proteins and their annotated locations for NP_036331.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (1).
    Source sequence(s)
    AF093097, AK314148, AL139286, BC063275, CR936823, HY052874
    Consensus CDS
    CCDS398.1
    UniProtKB/Swiss-Prot
    Q9UL18
    UniProtKB/TrEMBL
    B2RAD8
    Related
    ENSP00000362300.4, ENST00000373204.6
    Conserved Domains (4) summary
    cd02846
    Location:225345
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:390815
    Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
    pfam08699
    Location:176224
    ArgoL1; Argonaute linker 1 domain
    pfam16486
    Location:34164
    ArgoN; N-terminal domain of argonaute

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000001.11 Reference GRCh38.p13 Primary Assembly

    Range
    35869808..35930532
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017001019.1XP_016856508.1  protein argonaute-1 isoform X5

    Conserved Domains (3) summary
    cd02846
    Location:74194
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:239664
    Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
    pfam08699
    Location:2573
    ArgoL1; Argonaute linker 1 domain
  2. XM_017001016.1XP_016856505.1  protein argonaute-1 isoform X1

    Conserved Domains (5) summary
    PLN03202
    Location:26875
    PLN03202; protein argonaute; Provisional
    cd02846
    Location:225345
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:390834
    Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...
    pfam08699
    Location:175224
    ArgoL1; Argonaute linker 1 domain
    pfam16486
    Location:26164
    ArgoN; N-terminal domain of argonaute
  3. XM_011541236.2XP_011539538.1  protein argonaute-1 isoform X3

    See identical proteins and their annotated locations for XP_011539538.1

    Conserved Domains (4) summary
    cd02846
    Location:225345
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:390818
    Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
    pfam08699
    Location:176224
    ArgoL1; Argonaute linker 1 domain
    pfam16486
    Location:34164
    ArgoN; N-terminal domain of argonaute
  4. XM_017001017.1XP_016856506.1  protein argonaute-1 isoform X2

    Conserved Domains (5) summary
    PLN03202
    Location:26872
    PLN03202; protein argonaute; Provisional
    cd02846
    Location:225345
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:390831
    Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...
    pfam08699
    Location:175224
    ArgoL1; Argonaute linker 1 domain
    pfam16486
    Location:26164
    ArgoN; N-terminal domain of argonaute
  5. XM_017001018.1XP_016856507.1  protein argonaute-1 isoform X4

    Conserved Domains (3) summary
    cd02846
    Location:74194
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:239683
    Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...
    pfam08699
    Location:2473
    ArgoL1; Argonaute linker 1 domain
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