p2rx4a purinergic receptor P2X, ligand-gated ion channel, 4a [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: p2rx4aprovided by ZNC
Official Full Name: purinergic receptor P2X, ligand-gated ion channel, 4aprovided by ZNC
Primary source: ZFIN:ZDB-GENE-020806-2
See related: Ensembl:ENSDARG00000044752 AllianceGenome:ZFIN:ZDB-GENE-020806-2
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: p2x4; p2rx4; wu:fi03f02
Summary: Enables extracellularly ATP-gated monoatomic cation channel activity; identical protein binding activity; and ribonucleotide binding activity. Acts upstream of or within calcium ion transport. Located in membrane. Part of monoatomic ion channel complex. Orthologous to human P2RX4 (purinergic receptor P2X 4). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs: all
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Genomic context

Location:: chromosome: 21

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2025_04	current	GRCz12tu (GCF_049306965.1)	21	NC_133196.1 (17131766..17141083)
RS_2024_08	previous assembly	GRCz11 (GCF_000002035.6)	21	NC_007132.7 (15723206..15732398)

Chromosome 21 - NC_133196.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Our NMR analyses also revealed that the transmembrane region adopts the open conformation in the state bound to the inhibitor trinitrophenyl-ATP, and thus the antagonism is due to the closure of ion pathways, except for the pore in the transmembrane region: i.e., the lateral cation access in the extracellular region.
Title: Conductance of P2X4 purinergic receptor is determined by conformational equilibrium in the transmembrane region.
ATP-recognition of P2X4 receptors
Title: Inherent dynamics of head domain correlates with ATP-recognition of P2X4 receptors: insights gained from molecular simulations.
a property that is essential for purinergic sensory signaling. Apo and ATP-bound X-ray structures of the detergent-solubilized zebrafish P2X4 receptor provide a blueprint for receptor mechanisms
Title: Inter- and intrasubunit interactions between transmembrane helices in the open state of P2X receptor channels.
crystal structure of the zebrafish P2X4 receptor in complex with ATP and a new structure of the apo receptor
Title: Molecular mechanism of ATP binding and ion channel activation in P2X receptors.
These computational studies produced the first complete model, supported by experimental data, for how ATP binding triggers activation of a P2X receptor.
Title: Gating mechanism of a P2X4 receptor developed from normal mode analysis and molecular dynamics simulations.
Comparison of the acid-sensing ion channel structure with the ATP-gated P2X(4) receptor reveals similarity in pore architecture and aqueous vestibules
Title: Pore architecture and ion sites in acid-sensing ion channels and P2X receptors.
Cloning and characterization of zebrafish P2X4 and P2X5.
Title: Cloning and characterization of two novel zebrafish P2X receptor subunits.

Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-gated ion channel activity	IDA Inferred from Direct Assay more info	PubMed
enables CTP binding	IDA Inferred from Direct Assay more info	PubMed
enables extracellularly ATP-gated monoatomic cation channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables extracellularly ATP-gated monoatomic cation channel activity	IDA Inferred from Direct Assay more info	PubMed
enables extracellularly ATP-gated monoatomic cation channel activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ligand-gated monoatomic ion channel activity	IDA Inferred from Direct Assay more info	PubMed
enables monoatomic ion channel activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables purine nucleotide binding	IDA Inferred from Direct Assay more info	PubMed
enables purinergic nucleotide receptor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in calcium ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within calcium ion transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within monoatomic cation transmembrane transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within monoatomic cation transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within monoatomic ion transmembrane transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within monoatomic ion transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to ATP	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in lysosomal membrane	IEA Inferred from Electronic Annotation more info
located_in lysosome	IEA Inferred from Electronic Annotation more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
part_of monoatomic ion channel complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
part_of transmembrane transporter complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: P2X purinoceptor 4a

Names: etID14865.21; zP2X4.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.