Smad3 SMAD family member 3 [ Rattus norvegicus (Norway rat) ]

Gene ID: 25631, updated on 3-Jun-2026

Summary

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Official Symbol: Smad3provided by RGD
Official Full Name: SMAD family member 3provided by RGD
Primary source: RGD:3032
See related: Ensembl:ENSRNOG00000008620 AllianceGenome:RGD:3032
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: mad3; Madh3; Smad 3
Summary: Enables several functions, including DNA-binding transcription factor activity; RNA polymerase II transcription regulatory region sequence-specific DNA binding activity; and beta-catenin binding activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of cellular component biogenesis; and regulation of macromolecule metabolic process. Located in nucleus. Part of transcription regulator complex. Used to study cocaine dependence and pre-malignant neoplasm. Biomarker of alcoholic cardiomyopathy and alcoholic liver cirrhosis. Human ortholog(s) of this gene implicated in Loeys-Dietz syndrome 3; Lynch syndrome; and pancreatic cancer. Orthologous to human SMAD3 (SMAD family member 3). [provided by Alliance of Genome Resources, Apr 2025]
Expression: Biased expression in Muscle (RPKM 301.2), Kidney (RPKM 125.7) and 9 other tissues See more
Orthologs: all
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Genomic context

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See Smad3 in Genome Data Viewer

Location:: 8q24

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	8	NC_086026.1 (73022204..73132324, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	8	NC_051343.1 (64126829..64236960, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	8	NC_005107.4 (68569530..68678349, complement)

Chromosome 8 - NC_086026.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Zinc-Alpha-2-Glycoprotein Peptide Downregulates Type I and III Collagen Expression via Suppression of TGF-beta and p-Smad 2/3 Pathway in Keloid Fibroblasts and Rat Incisional Model.
Title: Zinc-Alpha-2-Glycoprotein Peptide Downregulates Type I and III Collagen Expression via Suppression of TGF-β and p-Smad 2/3 Pathway in Keloid Fibroblasts and Rat Incisional Model.
SIRT3 sulfhydration using hydrogen sulfide inhibited angiotensin II-induced atrial fibrosis and vulnerability to atrial fibrillation via suppression of the TGF-beta1/smad2/3 signalling pathway.
Title: SIRT3 sulfhydration using hydrogen sulfide inhibited angiotensin II-induced atrial fibrosis and vulnerability to atrial fibrillation via suppression of the TGF-β1/smad2/3 signalling pathway.
Superoxide dismutase 2 scavenges ROS to promote osteogenic differentiation of human periodontal ligament stem cells by regulating Smad3 in alveolar bone-defective rats.
Title: Superoxide dismutase 2 scavenges ROS to promote osteogenic differentiation of human periodontal ligament stem cells by regulating Smad3 in alveolar bone-defective rats.
Brachyury promotes extracellular matrix synthesis through transcriptional regulation of Smad3 in nucleus pulposus.
Title: Brachyury promotes extracellular matrix synthesis through transcriptional regulation of Smad3 in nucleus pulposus.
Recombinant Slit2 attenuates tracheal fibroblast activation in benign central airway obstruction by inhibiting the TGF-beta1/Smad3 signaling pathway.
Title: Recombinant Slit2 attenuates tracheal fibroblast activation in benign central airway obstruction by inhibiting the TGF-β1/Smad3 signaling pathway.
miR-135a Regulates Atrial Fibrillation by Targeting Smad3.
Title: miR-135a Regulates Atrial Fibrillation by Targeting Smad3.
The expression and phosphorylation of SMAD3 protein in microglia and astrocytes of the rat hippocampus after transient global cerebral ischemia.
Title: The expression and phosphorylation of SMAD3 protein in microglia and astrocytes of the rat hippocampus after transient global cerebral ischemia.
Hyperoxia induces alveolar epithelial cell apoptosis by regulating mitochondrial function through small mothers against decapentaplegic 3 (SMAD3) and extracellular signal-regulated kinase 1/2 (ERK1/2).
Title: Hyperoxia induces alveolar epithelial cell apoptosis by regulating mitochondrial function through small mothers against decapentaplegic 3 (SMAD3) and extracellular signal-regulated kinase 1/2 (ERK1/2).
Reduction of Smad2 caused by oxidative stress leads to necrotic death of hypertrophic chondrocytes associated with an endemic osteoarthritis.
Title: Reduction of Smad2 caused by oxidative stress leads to necrotic death of hypertrophic chondrocytes associated with an endemic osteoarthritis.
Zishen Qingre Tongluo Formula Improves Renal Fatty Acid Oxidation and Alleviated Fibrosis via the Regulation of the TGF-beta1/Smad3 Signaling Pathway in Hyperuricemic Nephrology Rats.
Title: Zishen Qingre Tongluo Formula Improves Renal Fatty Acid Oxidation and Alleviated Fibrosis via the Regulation of the TGF-β1/Smad3 Signaling Pathway in Hyperuricemic Nephrology Rats.

Submit: New GeneRIF Correction See all GeneRIFs (137)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH130799 (e-PCR)
- UniSTS:214083

Smad3 (e-PCR), detects polymorphism
- UniSTS:466571

RH138729 (e-PCR)
- UniSTS:220816

RH143351 (e-PCR)
- UniSTS:232598

PE5L-LSB (e-PCR)
- UniSTS:9921

Madh3 (e-PCR), detects polymorphism
- UniSTS:143810

Madh3 (e-PCR), detects polymorphism
- UniSTS:143811

Madh3 (e-PCR), detects polymorphism
- UniSTS:143812

Madh3 (e-PCR), detects polymorphism
- UniSTS:143814

RH143448 (e-PCR)
- UniSTS:232695

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables DEAD/H-box RNA helicase binding	IEA Inferred from Electronic Annotation more info
enables DEAD/H-box RNA helicase binding	ISO Inferred from Sequence Orthology more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
contributes_to DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription repressor activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription repressor activity	ISO Inferred from Sequence Orthology more info
enables I-SMAD binding	IBA Inferred from Biological aspect of Ancestor more info
enables R-SMAD binding	IEA Inferred from Electronic Annotation more info
enables R-SMAD binding	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables bHLH transcription factor binding	IEA Inferred from Electronic Annotation more info
enables bHLH transcription factor binding	ISO Inferred from Sequence Orthology more info
enables beta-catenin binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin DNA binding	IEA Inferred from Electronic Annotation more info
enables chromatin DNA binding	ISO Inferred from Sequence Orthology more info
enables chromatin binding	IEA Inferred from Electronic Annotation more info
enables chromatin binding	ISO Inferred from Sequence Orthology more info
enables cis-regulatory region sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables cis-regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables cis-regulatory region sequence-specific DNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables co-SMAD binding	IEA Inferred from Electronic Annotation more info
enables co-SMAD binding	ISO Inferred from Sequence Orthology more info
enables collagen binding	IEA Inferred from Electronic Annotation more info
enables collagen binding	ISO Inferred from Sequence Orthology more info
enables double-stranded DNA binding	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA binding	ISO Inferred from Sequence Orthology more info
enables enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables nuclear glucocorticoid receptor binding	IEA Inferred from Electronic Annotation more info
enables nuclear glucocorticoid receptor binding	ISO Inferred from Sequence Orthology more info
enables nuclear mineralocorticoid receptor binding	IEA Inferred from Electronic Annotation more info
enables nuclear mineralocorticoid receptor binding	ISO Inferred from Sequence Orthology more info
enables nuclear receptor binding	IEA Inferred from Electronic Annotation more info
enables nuclear receptor binding	ISO Inferred from Sequence Orthology more info
enables phosphatase binding	IEA Inferred from Electronic Annotation more info
enables phosphatase binding	ISO Inferred from Sequence Orthology more info
enables promoter-specific chromatin binding	IEA Inferred from Electronic Annotation more info
enables promoter-specific chromatin binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein kinase binding	IEA Inferred from Electronic Annotation more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables sterol response element binding	IEA Inferred from Electronic Annotation more info
enables sterol response element binding	ISO Inferred from Sequence Orthology more info
enables transcription cis-regulatory region binding	IEA Inferred from Electronic Annotation more info
enables transcription cis-regulatory region binding	ISO Inferred from Sequence Orthology more info
enables transcription coactivator binding	IEA Inferred from Electronic Annotation more info
enables transcription coactivator binding	ISO Inferred from Sequence Orthology more info
enables transcription corepressor binding	IEA Inferred from Electronic Annotation more info
enables transcription corepressor binding	ISO Inferred from Sequence Orthology more info
enables transforming growth factor beta receptor binding	IEA Inferred from Electronic Annotation more info
enables transforming growth factor beta receptor binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in JNK cascade	IEA Inferred from Electronic Annotation more info
involved_in JNK cascade	ISO Inferred from Sequence Orthology more info
involved_in MAPK cascade	IEA Inferred from Electronic Annotation more info
involved_in MAPK cascade	ISO Inferred from Sequence Orthology more info
involved_in SMAD protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in SMAD protein signal transduction	IEA Inferred from Electronic Annotation more info
involved_in SMAD protein signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of SMAD protein signal transduction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within SMAD protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in SMAD protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in T cell activation	IEA Inferred from Electronic Annotation more info
involved_in T cell activation	ISO Inferred from Sequence Orthology more info
involved_in activin receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in activin receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in activin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in adrenal gland development	IDA Inferred from Direct Assay more info	PubMed
involved_in anatomical structure morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in apoptotic signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in cell-cell junction organization	IEA Inferred from Electronic Annotation more info
involved_in cell-cell junction organization	ISO Inferred from Sequence Orthology more info
involved_in cellular response to glucose stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to platelet-derived growth factor stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to transforming growth factor beta stimulus	IEA Inferred from Electronic Annotation more info
involved_in cellular response to transforming growth factor beta stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to transforming growth factor beta stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to virus	IEA Inferred from Electronic Annotation more info
involved_in cellular response to virus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within developmental growth	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within embryonic cranial skeleton morphogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within embryonic foregut morphogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within embryonic pattern specification	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within endoderm development	ISO Inferred from Sequence Orthology more info
involved_in extrinsic apoptotic signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in extrinsic apoptotic signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in extrinsic apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within gastrulation	ISO Inferred from Sequence Orthology more info
involved_in gene expression	IEA Inferred from Electronic Annotation more info
involved_in gene expression	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within heart looping	ISO Inferred from Sequence Orthology more info
involved_in immune response	IEA Inferred from Electronic Annotation more info
involved_in immune response	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within immune system development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within in utero embryonic development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within lens fiber cell differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within liver development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within mesoderm formation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of cardiac muscle hypertrophy in response to stress	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of cell growth	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cell growth	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell population proliferation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of cell population proliferation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cytosolic calcium ion concentration	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cytosolic calcium ion concentration	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of fat cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of fat cell differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of negative regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of inflammatory response	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of lung blood pressure	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of miRNA transcription	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of miRNA transcription	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of ossification	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of ossification	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of osteoblast differentiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of osteoblast proliferation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of osteoblast proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of wound healing	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of wound healing	ISO Inferred from Sequence Orthology more info
involved_in nodal signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in nodal signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within osteoblast development	ISO Inferred from Sequence Orthology more info
involved_in osteoblast differentiation	IEA Inferred from Electronic Annotation more info
involved_in osteoblast differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within paraxial mesoderm morphogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within pericardium development	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of SMAD protein signal transduction	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of SMAD protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of alkaline phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of bone mineralization	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of canonical Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of chondrocyte differentiation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of chondrocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of epithelial to mesenchymal transition	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of epithelial to mesenchymal transition	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of extracellular matrix assembly	IEA Inferred from Electronic Annotation more info
acts_upstream_of positive regulation of extracellular matrix assembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of extracellular matrix assembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of focal adhesion assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interleukin-1 beta production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of miRNA transcription	IEA Inferred from Electronic Annotation more info
acts_upstream_of positive regulation of miRNA transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of miRNA transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of nitric oxide biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of nitric oxide biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of nitric oxide biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of positive chemotaxis	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of stress fiber assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transforming growth factor beta3 production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein stabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in regulation of dendritic spine morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of epithelial cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in regulation of immune response	IEA Inferred from Electronic Annotation more info
involved_in regulation of immune response	ISO Inferred from Sequence Orthology more info
involved_in regulation of mitochondrial membrane potential	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of striated muscle tissue development	ISO Inferred from Sequence Orthology more info
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of transforming growth factor beta receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in regulation of transforming growth factor beta2 production	IEA Inferred from Electronic Annotation more info
involved_in regulation of transforming growth factor beta2 production	ISO Inferred from Sequence Orthology more info
involved_in release of cytochrome c from mitochondria	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to alcohol	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of response to angiotensin	ISO Inferred from Sequence Orthology more info
involved_in response to cocaine	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to gamma radiation	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to hypoxia	IEA Inferred from Electronic Annotation more info
involved_in response to hypoxia	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to transforming growth factor beta	ISO Inferred from Sequence Orthology more info
involved_in signal transduction involved in regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within skeletal system development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within somitogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within thyroid gland development	ISO Inferred from Sequence Orthology more info
involved_in transdifferentiation	IDA Inferred from Direct Assay more info	PubMed
involved_in transforming growth factor beta receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in transforming growth factor beta receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in transforming growth factor beta receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within transforming growth factor beta receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in transforming growth factor beta receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in trophoblast cell migration	IEA Inferred from Electronic Annotation more info
involved_in trophoblast cell migration	ISO Inferred from Sequence Orthology more info
involved_in ureteric bud development	IEA Inferred from Electronic Annotation more info
involved_in ureteric bud development	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of SMAD protein complex	IEA Inferred from Electronic Annotation more info
part_of SMAD protein complex	ISO Inferred from Sequence Orthology more info
part_of SMAD protein complex	ISS Inferred from Sequence or Structural Similarity more info
located_in chromatin	IEA Inferred from Electronic Annotation more info
located_in chromatin	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
part_of heteromeric SMAD protein complex	IBA Inferred from Biological aspect of Ancestor more info
part_of heteromeric SMAD protein complex	IEA Inferred from Electronic Annotation more info
part_of heteromeric SMAD protein complex	ISO Inferred from Sequence Orthology more info
part_of homomeric SMAD protein complex	IEA Inferred from Electronic Annotation more info
located_in nuclear inner membrane	IEA Inferred from Electronic Annotation more info
located_in nuclear inner membrane	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	EXP Inferred from Experiment more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
part_of protein-DNA complex	IEA Inferred from Electronic Annotation more info
part_of protein-DNA complex	ISO Inferred from Sequence Orthology more info
part_of signaling receptor complex	IEA Inferred from Electronic Annotation more info
part_of signaling receptor complex	ISO Inferred from Sequence Orthology more info
part_of transcription regulator complex	IDA Inferred from Direct Assay more info	PubMed
part_of transcription regulator complex	IEA Inferred from Electronic Annotation more info
part_of transcription regulator complex	ISO Inferred from Sequence Orthology more info
part_of transcription repressor complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: SMAD family member 3; mothers against decapentaplegic homolog 3

Names: MAD (mothers against decapentaplegic, Drosophila) homolog 3; MAD homolog 3; mothers against DPP homolog 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_013095.3 → NP_037227.1 SMAD family member 3

See identical proteins and their annotated locations for NP_037227.1

Status: VALIDATED

Source sequence(s)

BC064437, JAXUCZ010000008

UniProtKB/Swiss-Prot

P84025

UniProtKB/TrEMBL

A6J5A4

Related

ENSRNOP00000037346.3, ENSRNOT00000039730.6

Conserved Domains (2) summary

cd10491
Location:8 → 132

MH1_SMAD_2_3; N-terminal Mad Homology 1 (MH1) domain in SMAD2 and SMAD3

cd10985
Location:224 → 414

MH2_SMAD_2_3; C-terminal Mad Homology 2 (MH2) domain in SMAD2 and SMAD3

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086026.1 Reference GRCr8

Range

73022204..73132324 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039080897.2 → XP_038936825.1 mothers against decapentaplegic homolog 3 isoform X2

Conserved Domains (2) summary

cd10985
Location:119 → 309

MH2_SMAD_2_3; C-terminal Mad Homology 2 (MH2) domain in SMAD2 and SMAD3

cl00055
Location:1 → 27

MH1; N-terminal Mad Homology 1 (MH1) domain
XM_039080896.2 → XP_038936824.1 mothers against decapentaplegic homolog 3 isoform X1

UniProtKB/TrEMBL

A0A8I5ZS20

Related

ENSRNOP00000081624.2, ENSRNOT00000099327.2

Conserved Domains (2) summary

cd10985
Location:202 → 392

MH2_SMAD_2_3; C-terminal Mad Homology 2 (MH2) domain in SMAD2 and SMAD3

cl00055
Location:48 → 110

MH1; N-terminal Mad Homology 1 (MH1) domain
XM_008766216.4 → XP_008764438.1 mothers against decapentaplegic homolog 3 isoform X3

See identical proteins and their annotated locations for XP_008764438.1

Conserved Domains (1) summary

cd10985
Location:29 → 219

MH2_SMAD_2_3; C-terminal Mad Homology 2 (MH2) domain in SMAD2 and SMAD3