RNF144B ring finger protein 144B [Homo sapiens (human)] - Gene

Summary

Official Symbol: RNF144Bprovided by HGNC
Official Full Name: ring finger protein 144Bprovided by HGNC
Primary source: HGNC:HGNC:21578
See related: Ensembl:ENSG00000137393 MIM:618869; AllianceGenome:HGNC:21578
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: PIR2; IBRDC2; p53RFP; bA528A10.3
Summary: Enables ubiquitin-protein transferase activity. Involved in negative regulation of apoptotic process and ubiquitin-dependent protein catabolic process. Located in mitochondrial membrane. [provided by Alliance of Genome Resources, Jul 2025]
Expression: Broad expression in thyroid (RPKM 30.0), lung (RPKM 13.0) and 20 other tissues See more
Orthologs: all
: Try the new Gene page
Try the new Transcripts and proteins table

Genomic context

Location:: 6p22.3

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2025_08	current	GRCh38.p14 (GCF_000001405.40)	6	NC_000006.12 (18387350..18468870)
RS_2025_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	6	NC_060930.1 (18259855..18340593)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	6	NC_000006.11 (18387581..18469101)

Chromosome 6 - NC_000006.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help

See details

Project title: Tissue-specific circular RNA induction during human fetal development
Description: 35 human fetal samples from 6 tissues (3 - 7 replicates per tissue) collected between 10 and 20 weeks gestational time were sequenced using Illumina TruSeq Stranded Total RNA
BioProject: PRJNA270632
Publication: PMID 26076956
Analysis date: Mon Apr 2 22:54:59 2018

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The TP53-activated E3 ligase RNF144B is a tumour suppressor that prevents genomic instability.
Title: The TP53-activated E3 ligase RNF144B is a tumour suppressor that prevents genomic instability.
RNF144B stimulates the proliferation and inhibits the apoptosis of human spermatogonial stem cells via the FCER2/NOTCH2/HES1 pathway and its abnormality is associated with azoospermia.
Title: RNF144B stimulates the proliferation and inhibits the apoptosis of human spermatogonial stem cells via the FCER2/NOTCH2/HES1 pathway and its abnormality is associated with azoospermia.
Our study identifies that RNF144B interaction with TBK1 is sufficient to inactivate TBK1 and delineates a previously unrecognized role for RNF144B in innate immune responses.
Title: RNF144B inhibits LPS-induced inflammatory responses via binding TBK1.
Inactivation of GSK3beta activity is associated with loss of PIR2 protein and consequent inhibition of cell proliferation in endometrial cancer cells.
Title: Pir2/Rnf144b is a potential endometrial cancer biomarker that promotes cell proliferation.
Low RNF144B expression is associated with gastric cancer.
Title: miR-100 antagonism triggers apoptosis by inhibiting ubiquitination-mediated p53 degradation.
findings substantiate PIR2/Rnf144b as a potentially critical component of epithelial homeostasis, acting downstream of DeltaNp63alpha to regulate cellular levels of p21(WAF1/CIP1) and DeltaNp63alpha
Title: PIR2/Rnf144B regulates epithelial homeostasis by mediating degradation of p21WAF1 and p63.
PIR2, by being induced by TAp73 and degrading DeltaNp73, differentially regulates TAp73/DeltaNp73 stability.
Title: Differential control of TAp73 and DeltaNp73 protein stability by the ring finger ubiquitin ligase PIR2.
PIR2/RNF144B/IBRDC2 regulates the stability of TAp73 and DNp73.
Title: Differential control of TAp73 and DeltaNp73 protein stability by the ring finger ubiquitin ligase PIR2.
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
IBRDC2, an IBR-type RING-finger E3 ubiquitin ligase, regulates the levels of Bax and protects cells from unprompted Bax. activation and cell death.
Title: IBRDC2, an IBR-type E3 ubiquitin ligase, is a regulatory factor for Bax and apoptosis activation.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Phenotypes

Go to the top of the page Help

Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide association study link novel loci to endometriosis. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

Go to the top of the page Help

See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

G32680 (e-PCR)
- UniSTS:117262

RH12676 (e-PCR)
- UniSTS:68676

STS-R00513 (e-PCR)
- UniSTS:28751

A003N40 (e-PCR)
- UniSTS:17810

A009N14 (e-PCR)
- UniSTS:53651

G20359 (e-PCR)
- UniSTS:58862

A005N35 (e-PCR)
- UniSTS:58863

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin conjugating enzyme binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	EXP Inferred from Experiment more info	PubMed
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in negative regulation of apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	EXP Inferred from Experiment more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	TAS Traceable Author Statement more info
is_active_in mitochondrial membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	IDA Inferred from Direct Assay more info
part_of ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of ubiquitin ligase complex	IC Inferred by Curator more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: E3 ubiquitin-protein ligase RNF144B

Names: IBR domain containing 2; IBR domain-containing protein 2; p53-inducible RING finger protein

NP_877434.2

EC 2.3.2.31

XP_047274550.1

EC 2.3.2.31

XP_054211039.1

EC 2.3.2.31

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_182757.4 → NP_877434.2 E3 ubiquitin-protein ligase RNF144B

See identical proteins and their annotated locations for NP_877434.2

Status: VALIDATED

Source sequence(s)

AK096832, AL138725

Consensus CDS

CCDS34345.1

UniProtKB/Swiss-Prot

B3KUA8, B4DZI2, Q5TB85, Q6P4Q0, Q7Z419, Q8N3R7, Q9BX38

UniProtKB/TrEMBL

A0ACI8QDL2

Related

ENSP00000259939.4, ENST00000259939.4

Conserved Domains (3) summary

smart00647
Location:101 → 166

IBR; In Between Ring fingers

cd16778
Location:28 → 84

mRING-HC-C4C4_RBR_RNF144B; Modified RING finger, HC subclass (C4C4-type), found in RING finger protein 144B (RNF144B)

pfam01485
Location:190 → 225

IBR; IBR domain, a half RING-finger domain