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Pc pyruvate carboxylase [ Rattus norvegicus (Norway rat) ]

Gene ID: 25104, updated on 4-Apr-2023

Summary

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Official Symbol
Pcprovided by RGD
Official Full Name
pyruvate carboxylaseprovided by RGD
Primary source
RGD:3262
See related
Ensembl:ENSRNOG00000019372 AllianceGenome:RGD:3262
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
Pcx
Summary
Enables ATP binding activity; biotin binding activity; and pyruvate carboxylase activity. Involved in carboxylic acid metabolic process; gluconeogenesis; and positive regulation of phospholipid biosynthetic process. Located in mitochondrion. Human ortholog(s) of this gene implicated in pyruvate carboxylase deficiency disease. Orthologous to human PC (pyruvate carboxylase). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in Kidney (RPKM 891.7), Liver (RPKM 758.1) and 6 other tissues See more
Orthologs
human mouse all
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Genomic context

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Location:
1q43
Exon count:
26
Annotation release Status Assembly Chr Location
108 current mRatBN7.2 (GCF_015227675.2) 1 NC_051336.1 (201799374..201898412)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 1 NC_005100.4 (219759157..219859854)

Chromosome 1 - NC_051336.1Genomic Context describing neighboring genes Neighboring gene synaptotagmin 12 Neighboring gene solute carrier family 25 member 5, pseudogene 10 Neighboring gene hypothetical protein LOC689065 Neighboring gene leucine rich repeat and fibronectin type III domain containing 4 Neighboring gene TOP6B like initiator of meiotic double strand breaks Neighboring gene Ras converting CAAX endopeptidase 1 Neighboring gene ribosomal protein L7a, pseudogene 7

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. Multiple E-boxes in the distal promoter of the rat pyruvate carboxylase gene function as a glucose-responsive element. Wutthisathapornchai A, et al. PLoS One, 2014. PMID 25054881, Free PMC Article
  2. Glucose induces expression of rat pyruvate carboxylase through a carbohydrate response element in the distal gene promoter. Pedersen KB, et al. Biochem J, 2010 Feb 9. PMID 20001964
  3. The role of pyruvate carboxylase in insulin secretion and proliferation in rat pancreatic beta cells. Xu J, et al. Diabetologia, 2008 Nov. PMID 18769905, Free PMC Article
  4. Impaired anaplerosis and insulin secretion in insulinoma cells caused by small interfering RNA-mediated suppression of pyruvate carboxylase. Hasan NM, et al. J Biol Chem, 2008 Oct 17. PMID 18697738, Free PMC Article
  5. Expression of pyruvate carboxylase in cultured oligodendroglial, microglial and ependymal cells. Murin R, et al. Neurochem Res, 2009 Mar. PMID 18686030

See all (39) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data indicate that glucose-mediated transcriptional activation of the pyruvate carboxylase (PC) gene is regulated by transcription factors.
    Title: Multiple E-boxes in the distal promoter of the rat pyruvate carboxylase gene function as a glucose-responsive element.
  2. Pyruvate carboxylase and fatty acid synthase were knocked down in insulin cells. Insulin secretion, phospholipid and cholesterol esters were lowered.
    Title: Knockdown of pyruvate carboxylase or fatty acid synthase lowers numerous lipids and glucose-stimulated insulin release in insulinoma cells.
  3. A carbohydrate response element in the distal gene promoter contributes to the glucose-mediated expression of pyruvate carboxylase.
    Title: Glucose induces expression of rat pyruvate carboxylase through a carbohydrate response element in the distal gene promoter.
  4. PC is not only expressed in cultured astroglial cells but also in cultured oligodendrocytes, microglial cells, and ependymocytes.
    Title: Expression of pyruvate carboxylase in cultured oligodendroglial, microglial and ependymal cells.
  5. The PC pathway in beta cells may play a key role in pyruvate metabolism, insulin secretion and cell proliferation.
    Title: The role of pyruvate carboxylase in insulin secretion and proliferation in rat pancreatic beta cells.
  6. PC is important for insulin secretion stimulated by noncarbohydrate insulin secretagogues
    Title: Impaired anaplerosis and insulin secretion in insulinoma cells caused by small interfering RNA-mediated suppression of pyruvate carboxylase.
  7. The results presented here show that Foxa2 and USFs regulate the distal promoter of the rat PC gene in a cell-specific manner.
    Title: Transcriptional regulation of the distal promoter of the rat pyruvate carboxylase gene by hepatocyte nuclear factor 3beta/Foxa2 and upstream stimulatory factors in insulinoma cells.
  8. beta-cells activate compensatory mechanisms in response to suppression of PC expression that prevent impairment of anaplerosis, pyruvate cycling, NAPDH production, and GSIS
    Title: Compensatory responses to pyruvate carboxylase suppression in islet beta-cells. Preservation of glucose-stimulated insulin secretion.
  9. Pyruvate carboxylase plays a role in the enhanced insulin secretion and beta-cell growth that characterize beta-cell adaptation to insulin resistance
    Title: beta-Cell adaptation to insulin resistance. Increased pyruvate carboxylase and malate-pyruvate shuttle activity in islets of nondiabetic Zucker fatty rats.
  10. Anaplerosis via pyruvate carboxylase is required for the fuel-induced rise in the ATP:ADP ratio in rat pancreatic islets.
    Title: Anaplerosis via pyruvate carboxylase is required for the fuel-induced rise in the ATP:ADP ratio in rat pancreatic islets.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables ATP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables biotin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables biotin binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables carboxylic acid binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables pyruvate carboxylase activity IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
enables pyruvate carboxylase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyruvate carboxylase activity IEA
Inferred from Electronic Annotation
more info
  
enables pyruvate carboxylase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables pyruvate carboxylase activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in NADH metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in NADH metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in NADP metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in NADP metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in gluconeogenesis IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
involved_in gluconeogenesis IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in gluconeogenesis IEA
Inferred from Electronic Annotation
more info
  
involved_in gluconeogenesis ISO
Inferred from Sequence Orthology
more info
  
involved_in lipid metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of gene expression IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of gene expression ISO
Inferred from Sequence Orthology
more info
  
involved_in oxaloacetate metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation by host of viral process IEA
Inferred from Electronic Annotation
more info
  
involved_in positive regulation by host of viral process ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of phospholipid biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in pyruvate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
involved_in pyruvate metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in pyruvate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in viral RNA genome packaging IEA
Inferred from Electronic Annotation
more info
  
involved_in viral RNA genome packaging ISO
Inferred from Sequence Orthology
more info
  
involved_in viral release from host cell IEA
Inferred from Electronic Annotation
more info
  
involved_in viral release from host cell ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in mitochondrial matrix ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrion IEA
Inferred from Electronic Annotation
more info
  
located_in mitochondrion ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
pyruvate carboxylase, mitochondrial
Names
PCB
pyruvic carboxylase
NP_036876.2
XP_006230753.1
XP_006230754.1
XP_006230755.1
XP_038957320.1
XP_038957321.1
XP_038957322.1
XP_038957326.1
XP_038957329.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_012744.3 → NP_036876.2  pyruvate carboxylase, mitochondrial precursor

    See identical proteins and their annotated locations for NP_036876.2

    Status: PROVISIONAL

    Source sequence(s)
    JACYVU010000045
    UniProtKB/Swiss-Prot
    P52873, Q64555
    Related
    ENSRNOP00000026316.5, ENSRNOT00000026316.7
    Conserved Domains (1) summary
    PRK12999
    Location:33 → 1178
    PRK12999; pyruvate carboxylase; Reviewed

RefSeqs of Annotated Genomes: Rattus norvegicus Annotation Release 108 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference mRatBN7.2 Primary Assembly

Genomic

  1. NC_051336.1 Reference mRatBN7.2 Primary Assembly

    Range
    201799374..201898412
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_039101394.1 → XP_038957322.1  pyruvate carboxylase, mitochondrial isoform X2

    UniProtKB/Swiss-Prot
    Q64555
    Conserved Domains (1) summary
    PRK12999
    Location:33 → 1178
    PRK12999; pyruvate carboxylase; Reviewed

  2. XM_006230691.4 → XP_006230753.1  pyruvate carboxylase, mitochondrial isoform X1

    See identical proteins and their annotated locations for XP_006230753.1

    UniProtKB/TrEMBL
    A0A8L2QDW8
    Conserved Domains (1) summary
    PRK12999
    Location:34 → 1179
    PRK12999; pyruvate carboxylase; Reviewed

  3. XM_039101393.1 → XP_038957321.1  pyruvate carboxylase, mitochondrial isoform X1

    UniProtKB/TrEMBL
    A0A8L2QDW8
    Conserved Domains (1) summary
    PRK12999
    Location:34 → 1179
    PRK12999; pyruvate carboxylase; Reviewed

  4. XM_039101392.1 → XP_038957320.1  pyruvate carboxylase, mitochondrial isoform X1

    UniProtKB/TrEMBL
    A0A8L2QDW8
    Conserved Domains (1) summary
    PRK12999
    Location:34 → 1179
    PRK12999; pyruvate carboxylase; Reviewed

  5. XM_039101398.1 → XP_038957326.1  pyruvate carboxylase, mitochondrial isoform X1

    UniProtKB/TrEMBL
    A0A8L2QDW8
    Related
    ENSRNOP00000075317.2, ENSRNOT00000082388.2
    Conserved Domains (1) summary
    PRK12999
    Location:34 → 1179
    PRK12999; pyruvate carboxylase; Reviewed

  6. XM_006230692.4 → XP_006230754.1  pyruvate carboxylase, mitochondrial isoform X2

    See identical proteins and their annotated locations for XP_006230754.1

    UniProtKB/Swiss-Prot
    P52873, Q64555
    Conserved Domains (1) summary
    PRK12999
    Location:33 → 1178
    PRK12999; pyruvate carboxylase; Reviewed

  7. XM_006230693.3 → XP_006230755.1  pyruvate carboxylase, mitochondrial isoform X2

    See identical proteins and their annotated locations for XP_006230755.1

    UniProtKB/Swiss-Prot
    P52873, Q64555
    Related
    ENSRNOP00000068757.1, ENSRNOT00000087432.2
    Conserved Domains (1) summary
    PRK12999
    Location:33 → 1178
    PRK12999; pyruvate carboxylase; Reviewed

  8. XM_039101401.1 → XP_038957329.1  pyruvate carboxylase, mitochondrial isoform X3

    Conserved Domains (1) summary
    cl39082
    Location:41 → 770
    PycA; Pyruvate carboxylase [Energy production and conversion]
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P52873.2 GenPept UniProtKB/Swiss-Prot:P52873

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