U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Nos3 nitric oxide synthase 3 [ Rattus norvegicus (Norway rat) ]

Gene ID: 24600, updated on 5-Mar-2024

Summary

Official Symbol
Nos3provided by RGD
Official Full Name
nitric oxide synthase 3provided by RGD
Primary source
RGD:3186
See related
Ensembl:ENSRNOG00000009348 AllianceGenome:RGD:3186
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
eNos
Summary
Enables several functions, including Hsp90 protein binding activity; beta-catenin binding activity; and calmodulin binding activity. Involved in several processes, including cellular response to magnesium ion; cellular response to mechanical stimulus; and cellular response to organonitrogen compound. Located in several cellular components, including caveola; nucleolus; and sarcolemma. Used to study artery disease (multiple); irritable bowel syndrome; ischemia (multiple); liver disease (multiple); and retinal disease (multiple). Biomarker of several diseases, including artery disease (multiple); kidney failure (multiple); liver disease (multiple); lung disease (multiple); and overnutrition (multiple). Human ortholog(s) of this gene implicated in several diseases, including Alzheimer's disease (multiple); artery disease (multiple); eye disease (multiple); reproductive organ cancer (multiple); and vasculitis (multiple). Orthologous to human NOS3 (nitric oxide synthase 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in Heart (RPKM 114.2), Spleen (RPKM 51.1) and 8 other tissues See more
Orthologs
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

See Nos3 in Genome Data Viewer
Location:
4q11
Exon count:
26
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 4 NC_086022.1 (11686088..11706604, complement)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 4 NC_051339.1 (10793834..10814170, complement)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 4 NC_005103.4 (7321908..7342404, complement)

Chromosome 4 - NC_086022.1Genomic Context describing neighboring genes Neighboring gene ATP binding cassette subfamily B member 8 Neighboring gene uncharacterized LOC120102208 Neighboring gene autophagy related 9B Neighboring gene potassium voltage-gated channel subfamily H member 2 Neighboring gene uncharacterized LOC108350647

Genomic regions, transcripts, and products

Expression

  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Pathways from PubChem

General gene information

Markers

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables FMN binding IBA
Inferred from Biological aspect of Ancestor
more info
 
enables FMN binding IEA
Inferred from Electronic Annotation
more info
 
enables Hsp90 protein binding IDA
Inferred from Direct Assay
more info
PubMed 
enables NADP binding IEA
Inferred from Electronic Annotation
more info
 
enables actin binding IDA
Inferred from Direct Assay
more info
PubMed 
enables actin monomer binding IEA
Inferred from Electronic Annotation
more info
 
enables actin monomer binding ISO
Inferred from Sequence Orthology
more info
 
enables arginine binding IEA
Inferred from Electronic Annotation
more info
 
enables arginine binding ISO
Inferred from Sequence Orthology
more info
 
enables beta-catenin binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables cadherin binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables calmodulin binding IDA
Inferred from Direct Assay
more info
PubMed 
enables calmodulin binding IEA
Inferred from Electronic Annotation
more info
 
enables flavin adenine dinucleotide binding IBA
Inferred from Biological aspect of Ancestor
more info
 
enables flavin adenine dinucleotide binding IEA
Inferred from Electronic Annotation
more info
 
enables heme binding IEA
Inferred from Electronic Annotation
more info
 
enables heme binding ISO
Inferred from Sequence Orthology
more info
 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
 
enables nitric-oxide synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
 
enables nitric-oxide synthase activity IDA
Inferred from Direct Assay
more info
PubMed 
enables nitric-oxide synthase activity IEA
Inferred from Electronic Annotation
more info
 
enables nitric-oxide synthase activity IMP
Inferred from Mutant Phenotype
more info
PubMed 
enables nitric-oxide synthase activity ISO
Inferred from Sequence Orthology
more info
 
enables nitric-oxide synthase binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables scaffold protein binding IEA
Inferred from Electronic Annotation
more info
 
enables scaffold protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables scaffold protein binding ISO
Inferred from Sequence Orthology
more info
 
enables tetrahydrobiopterin binding IEA
Inferred from Electronic Annotation
more info
 
enables tetrahydrobiopterin binding ISO
Inferred from Sequence Orthology
more info
 
Process Evidence Code Pubs
involved_in angiogenesis IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of_or_within angiogenesis ISO
Inferred from Sequence Orthology
more info
 
involved_in aortic valve morphogenesis IEA
Inferred from Electronic Annotation
more info
 
involved_in aortic valve morphogenesis ISO
Inferred from Sequence Orthology
more info
 
involved_in arginine catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in arginine catabolic process IEA
Inferred from Electronic Annotation
more info
 
involved_in arginine catabolic process ISO
Inferred from Sequence Orthology
more info
 
involved_in blood vessel diameter maintenance IEA
Inferred from Electronic Annotation
more info
 
involved_in blood vessel diameter maintenance ISO
Inferred from Sequence Orthology
more info
 
involved_in blood vessel remodeling IEA
Inferred from Electronic Annotation
more info
 
involved_in blood vessel remodeling ISO
Inferred from Sequence Orthology
more info
 
involved_in bone development IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in calcium ion transport IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of calcium ion transport ISO
Inferred from Sequence Orthology
more info
 
involved_in cellular response to cAMP IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in cellular response to growth factor stimulus IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in cellular response to heat IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in cellular response to high density lipoprotein particle stimulus IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in cellular response to insulin stimulus IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in cellular response to low-density lipoprotein particle stimulus IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in cellular response to magnesium ion IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in cellular response to mechanical stimulus IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in cellular response to organic cyclic compound IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in cellular response to rotenone IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in cellular response to transforming growth factor beta stimulus IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in cellular response to tumor necrosis factor IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in endocardial cushion morphogenesis IEA
Inferred from Electronic Annotation
more info
 
involved_in endocardial cushion morphogenesis ISO
Inferred from Sequence Orthology
more info
 
involved_in endothelial cell migration IEA
Inferred from Electronic Annotation
more info
 
involved_in endothelial cell migration ISO
Inferred from Sequence Orthology
more info
 
involved_in establishment of localization in cell IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of establishment of localization in cell ISO
Inferred from Sequence Orthology
more info
 
involved_in female pregnancy IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in homeostasis of number of cells within a tissue IEA
Inferred from Electronic Annotation
more info
 
involved_in homeostasis of number of cells within a tissue ISO
Inferred from Sequence Orthology
more info
 
involved_in in utero embryonic development IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of_or_within in utero embryonic development ISO
Inferred from Sequence Orthology
more info
 
involved_in lipopolysaccharide-mediated signaling pathway IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of_or_within lipopolysaccharide-mediated signaling pathway ISO
Inferred from Sequence Orthology
more info
 
involved_in lung development IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of_or_within lung development ISO
Inferred from Sequence Orthology
more info
 
involved_in negative regulation of biomineral tissue development IEA
Inferred from Electronic Annotation
more info
 
involved_in negative regulation of biomineral tissue development ISO
Inferred from Sequence Orthology
more info
 
involved_in negative regulation of blood pressure IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in negative regulation of calcium ion transport IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of_or_within negative regulation of calcium ion transport ISO
Inferred from Sequence Orthology
more info
 
involved_in negative regulation of cell population proliferation ISO
Inferred from Sequence Orthology
more info
 
involved_in negative regulation of muscle hyperplasia IEA
Inferred from Electronic Annotation
more info
 
involved_in negative regulation of muscle hyperplasia ISO
Inferred from Sequence Orthology
more info
 
involved_in negative regulation of potassium ion transport IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of_or_within negative regulation of potassium ion transport ISO
Inferred from Sequence Orthology
more info
 
involved_in negative regulation of smooth muscle cell proliferation IEA
Inferred from Electronic Annotation
more info
 
involved_in negative regulation of smooth muscle cell proliferation IMP
Inferred from Mutant Phenotype
more info
PubMed 
acts_upstream_of_or_within negative regulation of smooth muscle cell proliferation ISO
Inferred from Sequence Orthology
more info
 
involved_in nitric oxide biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in nitric oxide biosynthetic process IEA
Inferred from Electronic Annotation
more info
 
involved_in nitric oxide biosynthetic process IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in nitric oxide biosynthetic process IMP
Inferred from Mutant Phenotype
more info
PubMed 
acts_upstream_of_or_within nitric oxide biosynthetic process ISO
Inferred from Sequence Orthology
more info
 
involved_in nitric oxide biosynthetic process ISO
Inferred from Sequence Orthology
more info
 
involved_in nitric oxide mediated signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in nitric oxide metabolic process IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in ovulation from ovarian follicle IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of_or_within ovulation from ovarian follicle ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of angiogenesis IEA
Inferred from Electronic Annotation
more info
 
involved_in positive regulation of angiogenesis ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of apoptotic process IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in positive regulation of blood vessel endothelial cell migration IEA
Inferred from Electronic Annotation
more info
 
involved_in positive regulation of blood vessel endothelial cell migration ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of gene expression IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of_positive_effect positive regulation of gene expression ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of guanylate cyclase activity IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in potassium ion transport IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of potassium ion transport ISO
Inferred from Sequence Orthology
more info
 
involved_in pulmonary valve morphogenesis IEA
Inferred from Electronic Annotation
more info
 
involved_in pulmonary valve morphogenesis ISO
Inferred from Sequence Orthology
more info
 
involved_in regulation of nervous system process IEA
Inferred from Electronic Annotation
more info
 
involved_in regulation of nervous system process ISO
Inferred from Sequence Orthology
more info
 
involved_in regulation of sodium ion transport IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of_or_within regulation of sodium ion transport ISO
Inferred from Sequence Orthology
more info
 
involved_in regulation of systemic arterial blood pressure by endothelin IEA
Inferred from Electronic Annotation
more info
 
involved_in regulation of systemic arterial blood pressure by endothelin ISO
Inferred from Sequence Orthology
more info
 
involved_in regulation of the force of heart contraction by chemical signal IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of_or_within regulation of the force of heart contraction by chemical signal ISO
Inferred from Sequence Orthology
more info
 
involved_in removal of superoxide radicals IEA
Inferred from Electronic Annotation
more info
 
involved_in removal of superoxide radicals ISO
Inferred from Sequence Orthology
more info
 
involved_in response to L-arginine IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to activity IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to amino acid IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to axon injury IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to carbohydrate IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to corticosterone IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to cytokine IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to erythropoietin IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to estradiol IDA
Inferred from Direct Assay
more info
PubMed 
involved_in response to estradiol IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to ethanol IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to fluid shear stress IEA
Inferred from Electronic Annotation
more info
 
involved_in response to fluid shear stress ISO
Inferred from Sequence Orthology
more info
 
involved_in response to fructose IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to hormone IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in response to hyperoxia IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to hypoxia IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to ketone IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to lead ion IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to lipopolysaccharide IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in response to lipopolysaccharide IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to magnetism IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to mechanical stimulus IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to mercury ion IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to metal ion IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to nicotine IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to nutrient levels IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to organic cyclic compound IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to peptide IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to peptide hormone IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to salt IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to ultrasound IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to xenobiotic stimulus IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in retina development in camera-type eye IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in smooth muscle hyperplasia IEA
Inferred from Electronic Annotation
more info
 
involved_in smooth muscle hyperplasia ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within vasodilation ISO
Inferred from Sequence Orthology
more info
 
involved_in ventricular septum morphogenesis IEA
Inferred from Electronic Annotation
more info
 
involved_in ventricular septum morphogenesis ISO
Inferred from Sequence Orthology
more info
 
Component Evidence Code Pubs
located_in Golgi apparatus IEA
Inferred from Electronic Annotation
more info
 
located_in Golgi apparatus ISO
Inferred from Sequence Orthology
more info
 
located_in apical part of cell IDA
Inferred from Direct Assay
more info
PubMed 
located_in caveola IDA
Inferred from Direct Assay
more info
PubMed 
located_in caveola IEA
Inferred from Electronic Annotation
more info
 
located_in caveola ISO
Inferred from Sequence Orthology
more info
 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
 
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
 
located_in nucleus IDA
Inferred from Direct Assay
more info
PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
 
located_in nucleus ISO
Inferred from Sequence Orthology
more info
 
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
 
located_in plasma membrane ISO
Inferred from Sequence Orthology
more info
 
located_in sarcolemma IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
nitric oxide synthase 3
Names
EC-NOS
NOS type III
NOSIII
cNOS
constitutive NOS
endothelial NOS
endothelial nitric oxide synthase 3
nitric oxide synthase 3, endothelial cell
nitric oxide synthase, endothelial
NP_068610.1
XP_006235934.1

NCBI Reference Sequences (RefSeq)

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_021838.2NP_068610.1  nitric oxide synthase 3

    See identical proteins and their annotated locations for NP_068610.1

    Status: PROVISIONAL

    Source sequence(s)
    AB176831
    UniProtKB/TrEMBL
    A0A8I6A2Y1
    Related
    ENSRNOP00000086104.1, ENSRNOT00000118104.1
    Conserved Domains (5) summary
    COG0369
    Location:5111158
    CysJ; Sulfite reductase, alpha subunit (flavoprotein) [Inorganic ion transport and metabolism]
    cd00795
    Location:67479
    NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...
    pfam00258
    Location:521697
    Flavodoxin_1; Flavodoxin
    pfam01698
    Location:3061
    FLO_LFY; Floricaula / Leafy protein
    cl06868
    Location:7611163
    FNR_like; Ferredoxin reductase (FNR), an FAD and NAD(P) binding protein, was intially identified as a chloroplast reductase activity, catalyzing the electron transfer from reduced iron-sulfur protein ferredoxin to NADP+ as the final step in the electron transport ...

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086022.1 Reference GRCr8

    Range
    11686088..11706604 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006235872.3XP_006235934.1  nitric oxide synthase 3 isoform X1

    UniProtKB/TrEMBL
    A0A8I6A2Y1
    Conserved Domains (4) summary
    COG0369
    Location:4611108
    CysJ; Sulfite reductase, alpha subunit (flavoprotein) [Inorganic ion transport and metabolism]
    cd00795
    Location:17429
    NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...
    pfam00258
    Location:471647
    Flavodoxin_1; Flavodoxin
    cl06868
    Location:7111113
    FNR_like; Ferredoxin reductase (FNR), an FAD and NAD(P) binding protein, was intially identified as a chloroplast reductase activity, catalyzing the electron transfer from reduced iron-sulfur protein ferredoxin to NADP+ as the final step in the electron transport ...