Nefm neurofilament medium chain [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Nefmprovided by RGD
Official Full Name: neurofilament medium chainprovided by RGD
Primary source: RGD:3160
See related: Ensembl:ENSRNOG00000013916 AllianceGenome:RGD:3160
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Nfm; Nef3
Summary: Enables signaling receptor binding activity and toxic substance binding activity. Involved in several processes, including cellular response to estradiol stimulus; intermediate filament cytoskeleton organization; and nervous system development. Located in axon; neurofilament; and perikaryon. Used to study amyotrophic lateral sclerosis and congenital hypothyroidism. Biomarker of hypothyroidism; sciatic neuropathy; and transient cerebral ischemia. Human ortholog(s) of this gene implicated in Alzheimer's disease and human immunodeficiency virus infectious disease. Orthologous to human NEFM (neurofilament medium chain). [provided by Alliance of Genome Resources, Jul 2025]
Expression: Biased expression in Brain (RPKM 180.6) and Kidney (RPKM 17.7) See more
Orthologs: human mouse all
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Genomic context

Location:: 15p12

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	15	NC_086033.1 (46535857..46541161, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	15	NC_051350.1 (42360449..42365753, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	15	NC_005114.4 (44855307..44860604, complement)

Chromosome 15 - NC_086033.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

head domain phosphorylation of endogenous NF-M by forskolin-activated PKA inhibits NF-M tail domain phosphorylation in rat cortical neurons
Title: Phosphorylation of the head domain of neurofilament protein (NF-M): a factor regulating topographic phosphorylation of NF-M tail domain KSP sites in neurons.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH131013 (e-PCR)
- UniSTS:214296

D8S1927 (e-PCR)
- UniSTS:46910

UniSTS:465485 (e-PCR)
- UniSTS:465485

Nefm (e-PCR), detects polymorphism
- UniSTS:465485

Nfm (e-PCR), detects polymorphism
- UniSTS:144009

PMC125376P1 (e-PCR)
- UniSTS:270496

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed
enables signaling receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables structural constituent of cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
enables structural molecule activity	IEA Inferred from Electronic Annotation more info
enables toxic substance binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within axo-dendritic transport	ISO Inferred from Sequence Orthology more info
involved_in axon regeneration	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to estradiol stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to oxidative stress	IEP Inferred from Expression Pattern more info	PubMed
involved_in cerebral cortex development	IEP Inferred from Expression Pattern more info	PubMed
involved_in hippocampus development	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within intermediate filament bundle assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within intermediate filament cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in intermediate filament polymerization or depolymerization	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within microtubule cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in neurofilament bundle assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in neurofilament bundle assembly	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within neurofilament cytoskeleton organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of axon diameter	ISO Inferred from Sequence Orthology more info
involved_in response to acrylamide	IEP Inferred from Expression Pattern more info	PubMed
involved_in spinal cord development	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
is_active_in axon	IBA Inferred from Biological aspect of Ancestor more info
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in axon	IEA Inferred from Electronic Annotation more info
located_in axon	ISO Inferred from Sequence Orthology more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
is_active_in intermediate filament	IBA Inferred from Biological aspect of Ancestor more info
located_in intermediate filament	IDA Inferred from Direct Assay more info	PubMed
located_in intermediate filament	IEA Inferred from Electronic Annotation more info
located_in neurofibrillary tangle	ISO Inferred from Sequence Orthology more info
located_in neurofilament	IDA Inferred from Direct Assay more info	PubMed
located_in neurofilament	ISO Inferred from Sequence Orthology more info
located_in neuromuscular junction	ISO Inferred from Sequence Orthology more info
located_in neuron projection	ISO Inferred from Sequence Orthology more info
located_in perikaryon	IDA Inferred from Direct Assay more info	PubMed
located_in postsynaptic density	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic intermediate filament cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
is_active_in postsynaptic intermediate filament cytoskeleton	ISO Inferred from Sequence Orthology more info
is_active_in presynaptic intermediate filament cytoskeleton	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: neurofilament medium polypeptide

Names: 160 kDa neurofilament protein; NF-M; Neurofilament protein, middle polypeptide; neurofilament 3, medium; neurofilament triplet M protein; neurofilament, medium polypeptide

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.