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Klf8 Kruppel-like transcription factor 8 [ Mus musculus (house mouse) ]

Gene ID: 245671, updated on 5-Mar-2024

Summary

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Official Symbol
Klf8provided by MGI
Official Full Name
Kruppel-like transcription factor 8provided by MGI
Primary source
MGI:MGI:2442430
See related
Ensembl:ENSMUSG00000041649 AllianceGenome:MGI:2442430
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
BKLF3; ZNF74
Summary
Predicted to enable DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in negative regulation of transcription by RNA polymerase II. Predicted to be located in aggresome; cytosol; and nucleoplasm. Is expressed in early conceptus; inner cell mass; and liver. Orthologous to human KLF8 (Kruppel like factor 8). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in CNS E18 (RPKM 3.7), whole brain E14.5 (RPKM 2.3) and 16 other tissues See more
Orthologs
human all
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Genomic context

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See Klf8 in Genome Data Viewer
Location:
X F3; X 68.46 cM
Exon count:
14
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) X NC_000086.8 (152008335..152181921)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) X NC_000086.7 (153225339..153398925)

Chromosome X - NC_000086.8Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_48111 Neighboring gene forkhead box R2 Neighboring gene Ras-related GTP binding B Neighboring gene STARR-seq mESC enhancer starr_48113 Neighboring gene STARR-seq mESC enhancer starr_48114 Neighboring gene STARR-seq mESC enhancer starr_48116 Neighboring gene STARR-seq mESC enhancer starr_48117 Neighboring gene STARR-seq mESC enhancer starr_48118 Neighboring gene glyceraldehyde-3-phosphate dehydrogenase pseudogene Neighboring gene STARR-seq mESC enhancer starr_48121 Neighboring gene ubiquilin 2

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Krüppel-like transcription factor 8 (Klf8) is expressed and active in the neurons of the mouse brain. Dobrivojević M, et al. Gene, 2015 Oct 1. PMID 26071188
  2. Krüppel-like factor KLF8 plays a critical role in adipocyte differentiation. Lee H, et al. PLoS One, 2012. PMID 23285057, Free PMC Article
  3. A unique sequence in the N-terminal regulatory region controls the nuclear localization of KLF8 by cooperating with the C-terminal zinc-fingers. Mehta TS, et al. Cell Res, 2009 Sep. PMID 19488069
  4. Sumoylation delimits KLF8 transcriptional activity associated with the cell cycle regulation. Wei H, et al. J Biol Chem, 2006 Jun 16. PMID 16617055
  5. A network of Krüppel-like Factors (Klfs). Klf8 is repressed by Klf3 and activated by Klf1 in vivo. Eaton SA, et al. J Biol Chem, 2008 Oct 3. PMID 18687676, Free PMC Article

See all (31) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Klf8 is highly expressed in various regions of the mouse brain and in particular in the neurons, where it was localized in the cell nuclei.
    Title: Krüppel-like transcription factor 8 (Klf8) is expressed and active in the neurons of the mouse brain.
  2. KLF3 and KLF8 have overlapping roles in vivo and participate in the silencing of embryonic globin expression during development.
    Title: Generation of mice deficient in both KLF3/BKLF and KLF8 reveals a genetic interaction and a role for these factors in embryonic globin gene silencing.
  3. KLF8 is a key component of the transcription factor network that controls terminal differentiation during adipogenesis.
    Title: Krüppel-like factor KLF8 plays a critical role in adipocyte differentiation.
  4. PARP-1 as a novel KLF8-binding and -regulating protein and provided new insights into the mechanisms underlying the regulation of KLF8 nuclear localization, stability, and functions.
    Title: Identification of poly (ADP-ribose) polymerase-1 (PARP-1) as a novel Kruppel-like factor 8-interacting and -regulating protein.
  5. These results identified the KLF8 activation domain located between residues 101-260 where the well-conserved Q118 and Q248 are essential for recruiting p300 and PCAF to activate target gene transcription.
    Title: KLF8 recruits the p300 and PCAF co-activators to its amino terminal activation domain to activate transcription.
  6. KLF8 has two N terminal regulatory regions, one surrounding S165 and K171 and the other being two tandem ZFs, which are critical for the regulation of KLF8 nuclear localization and its cellular functions
    Title: A unique sequence in the N-terminal regulatory region controls the nuclear localization of KLF8 by cooperating with the C-terminal zinc-fingers.
  7. Klf8 is repressed by Klf3 and activated by Klf1 in vivo.
    Title: A network of Krüppel-like Factors (Klfs). Klf8 is repressed by Klf3 and activated by Klf1 in vivo.
  8. sumoylation of KLF8 negatively regulates its transcriptional activity and cellular functions
    Title: Sumoylation delimits KLF8 transcriptional activity associated with the cell cycle regulation.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Gene trapped (2) 
  • Endonuclease-mediated (2) 

General gene information

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Markers

Homology

Clone Names

  • A830097P10Rik

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables DNA binding ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
enables DNA-binding transcription factor activity, RNA polymerase II-specific IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA-binding transcription repressor activity, RNA polymerase II-specific ISO
Inferred from Sequence Orthology
more info
  
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding ISO
Inferred from Sequence Orthology
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in negative regulation of transcription by RNA polymerase II ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in aggresome ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Krueppel-like factor 8
Names
Kruppel-like factor 8

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001357183.2 → NP_001344112.1  Krueppel-like factor 8

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.
    Source sequence(s)
    AL671912
    Consensus CDS
    CCDS30481.1
    UniProtKB/Swiss-Prot
    Q8BLM0
    Related
    ENSMUSP00000044317.5, ENSMUST00000039545.5
    Conserved Domains (4) summary
    COG5048
    Location:268 → 338
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:272 → 294
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam13465
    Location:316 → 341
    zf-H2C2_2; Zinc-finger double domain
    cd21440
    Location:45 → 212
    KLF8_N; N-terminal domain of Kruppel-like factor 8

  2. NM_173780.6 → NP_776141.1  Krueppel-like factor 8

    See identical proteins and their annotated locations for NP_776141.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes the longer transcript. Variants 1 and 2 encode the same protein.
    Source sequence(s)
    AL671912, AL672293, AL805962
    Consensus CDS
    CCDS30481.1
    UniProtKB/Swiss-Prot
    Q8BLM0
    Related
    ENSMUSP00000108193.3, ENSMUST00000112574.9
    Conserved Domains (4) summary
    COG5048
    Location:268 → 338
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:272 → 294
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam13465
    Location:316 → 341
    zf-H2C2_2; Zinc-finger double domain
    cd21440
    Location:45 → 212
    KLF8_N; N-terminal domain of Kruppel-like factor 8

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000086.8 Reference GRCm39 C57BL/6J

    Range
    152008335..152181921
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017318503.1 → XP_017173992.1  Krueppel-like factor 8 isoform X3

    UniProtKB/Swiss-Prot
    Q8BLM0
    Conserved Domains (4) summary
    COG5048
    Location:268 → 338
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:272 → 294
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam13465
    Location:316 → 341
    zf-H2C2_2; Zinc-finger double domain
    cd21440
    Location:45 → 212
    KLF8_N; N-terminal domain of Kruppel-like factor 8

  2. XM_036161970.1 → XP_036017863.1  Krueppel-like factor 8 isoform X3

    UniProtKB/Swiss-Prot
    Q8BLM0
    Conserved Domains (4) summary
    COG5048
    Location:268 → 338
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:272 → 294
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam13465
    Location:316 → 341
    zf-H2C2_2; Zinc-finger double domain
    cd21440
    Location:45 → 212
    KLF8_N; N-terminal domain of Kruppel-like factor 8

  3. XM_017318505.3 → XP_017173994.1  Krueppel-like factor 8 isoform X3

    UniProtKB/Swiss-Prot
    Q8BLM0
    Conserved Domains (4) summary
    COG5048
    Location:268 → 338
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:272 → 294
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam13465
    Location:316 → 341
    zf-H2C2_2; Zinc-finger double domain
    cd21440
    Location:45 → 212
    KLF8_N; N-terminal domain of Kruppel-like factor 8

  4. XM_017318506.2 → XP_017173995.1  Krueppel-like factor 8 isoform X3

    UniProtKB/Swiss-Prot
    Q8BLM0
    Conserved Domains (4) summary
    COG5048
    Location:268 → 338
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:272 → 294
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam13465
    Location:316 → 341
    zf-H2C2_2; Zinc-finger double domain
    cd21440
    Location:45 → 212
    KLF8_N; N-terminal domain of Kruppel-like factor 8

  5. XM_006528859.4 → XP_006528922.1  Krueppel-like factor 8 isoform X3

    See identical proteins and their annotated locations for XP_006528922.1

    UniProtKB/Swiss-Prot
    Q8BLM0
    Conserved Domains (4) summary
    COG5048
    Location:268 → 338
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:272 → 294
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam13465
    Location:316 → 341
    zf-H2C2_2; Zinc-finger double domain
    cd21440
    Location:45 → 212
    KLF8_N; N-terminal domain of Kruppel-like factor 8

  6. XM_011247821.4 → XP_011246123.1  Krueppel-like factor 8 isoform X3

    See identical proteins and their annotated locations for XP_011246123.1

    UniProtKB/Swiss-Prot
    Q8BLM0
    Conserved Domains (4) summary
    COG5048
    Location:268 → 338
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:272 → 294
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam13465
    Location:316 → 341
    zf-H2C2_2; Zinc-finger double domain
    cd21440
    Location:45 → 212
    KLF8_N; N-terminal domain of Kruppel-like factor 8

  7. XM_006528852.5 → XP_006528915.1  Krueppel-like factor 8 isoform X2

    Conserved Domains (4) summary
    PHA03269
    Location:169 → 279
    PHA03269; envelope glycoprotein C; Provisional
    COG5048
    Location:358 → 428
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:362 → 384
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:420 → 442
    zf-C2H2; Zinc finger, C2H2 type

  8. XM_006528860.5 → XP_006528923.2  Krueppel-like factor 8 isoform X1

    Conserved Domains (4) summary
    COG5048
    Location:370 → 440
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:374 → 396
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:432 → 454
    zf-C2H2; Zinc finger, C2H2 type
    cd21440
    Location:147 → 314
    KLF8_N; N-terminal domain of Kruppel-like factor 8

  9. XM_030251350.2 → XP_030107210.1  Krueppel-like factor 8 isoform X3

    UniProtKB/Swiss-Prot
    Q8BLM0
    Conserved Domains (4) summary
    COG5048
    Location:268 → 338
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:272 → 294
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam13465
    Location:316 → 341
    zf-H2C2_2; Zinc-finger double domain
    cd21440
    Location:45 → 212
    KLF8_N; N-terminal domain of Kruppel-like factor 8

  10. XM_030251351.1 → XP_030107211.1  Krueppel-like factor 8 isoform X3

    UniProtKB/Swiss-Prot
    Q8BLM0
    Conserved Domains (4) summary
    COG5048
    Location:268 → 338
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:272 → 294
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam13465
    Location:316 → 341
    zf-H2C2_2; Zinc-finger double domain
    cd21440
    Location:45 → 212
    KLF8_N; N-terminal domain of Kruppel-like factor 8
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8BLM0.1 GenPept UniProtKB/Swiss-Prot:Q8BLM0

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