Mbp myelin basic protein [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Mbpprovided by RGD
Official Full Name: myelin basic proteinprovided by RGD
Primary source: RGD:3054
See related: EnsemblRapid:ENSRNOG00000016516
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Mbps
Summary: The protein encoded by the classic Mbp gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, Mbp-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long Mbp gene (otherwise called "Golli-Mbp") that contains 3 additional exons located upstream of the classic Mbp exons. Alternative splicing from the Golli and the Mbp transcription start sites gives rise to 2 sets of Mbp-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-Mbp, spliced in-frame to 1 or more Mbp exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to Mbp aa sequence. The second family of transcripts contain only Mbp exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the Mbp transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. Mutation of the Mbp gene is associated with the 'shiverer' and 'myelin deficient' phenotypes in mouse. [provided by RefSeq, Jul 2008]
Expression: Restricted expression toward (RPKM 4946.1) See more
Orthologs: human mouse all
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Genomic context

Location:: 18q12.3

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
108	current	mRatBN7.2 (GCF_015227675.2)	18	NC_051353.1 (75855878..75966404)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	18	NC_005117.4 (79326738..79437310)

Chromosome 18 - NC_051353.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

All pups with exposure to methadone demonstrated decreased expression of MBP in the cerebral cortex and hippocampus.In the cerebellum, PLP expression was downregulated without apparent alteration of CNP and MBP expression. PLP and MBP expression, but not CNP expression, were significantly inhibited in the brainstem.
Title: Diverse changes in myelin protein expression in rat brain after perinatal methadone exposure.
Gonadectomy prior to puberty onset resulted in a significant reduction of myelin basic protein levels in the orbitofrontal cortex in females but not males.
Title: Pubertal hormones mediate sex differences in levels of myelin basic protein in the orbitofrontal cortex of adult rats.
Angiostrongylus cantonensis-conditioned culture medium induced suppression of the Erk1/2-NF-kappaB signaling pathway leading to reduced MBP production in RSC96 Schwann cells.
Title: Angiostrongylus Cantonensis-Conditioned Culture Medium Induces Myelin Basic Protein Alterations via Erk1/2 and NF-κB Activation in Rat RSC96 Schwann Cells.
A role for the retrograde dynein/dynactin motor complex in anterograde mbp mRNA transport.
Title: Dynein/dynactin is necessary for anterograde transport of <i>Mbp</i> mRNA in oligodendrocytes and for myelination in vivo.
The aberrant phase transition of MBP molecules from their cohesive to soluble and non-adhesive state is a mechanism triggering myelin breakdown in neuromyelitis optica.
Title: Loss of Myelin Basic Protein Function Triggers Myelin Breakdown in Models of Demyelinating Diseases.
hindlimb unweighting treatment induced damage of L5 DRG neurons, which was correlated with decreased total MBP protein expression, increased dgen-MBP expression, and reduced GDNF protein and mRNA expression
Title: Effects of Hindlimb Unweighting on MBP and GDNF Expression and Morphology in Rat Dorsal Root Ganglia Neurons.
Bilateral common carotid artery occlusion led to loss of myelin basic protein expression.
Title: Characterization of White Matter Injury in a Rat Model of Chronic Cerebral Hypoperfusion.
Study demonstrated that downregulation of tau severely impairs process outgrowth, oligodendrocyte differentiation, and sheet formation, and that MBP mRNA transport to the distal cellular extensions is disturbed
Title: Downregulation of the microtubule associated protein tau impairs process outgrowth and myelin basic protein mRNA transport in oligodendrocytes.
Protein levels of MBP were significantly greater in orbital frontal cortex (OFC) but not dorsal striatum (dSTR) of females as compared to males, suggesting that myelination is increased in OFC but similar in dSTR of females as compared to males
Title: Sex differences in myelin-associated protein levels within and density of projections between the orbital frontal cortex and dorsal striatum of adult rats: implications for inhibitory control.
MBP cell membrane localization is altered in the presence of galactolipids.
Title: The lateral membrane organization and dynamics of myelin proteins PLP and MBP are dictated by distinct galactolipids and the extracellular matrix.

Submit: New GeneRIF Correction See all GeneRIFs (30)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH130523 (e-PCR)
- UniSTS:213807

D18Chm79 (e-PCR)
- UniSTS:518197

Mbp (e-PCR), detects polymorphism
- UniSTS:255289

BE106956 (e-PCR)
- UniSTS:261702

RH128788 (e-PCR)
- UniSTS:212096

RH141640 (e-PCR)
- UniSTS:223633

BF405689 (e-PCR)
- UniSTS:247954

BF402482 (e-PCR)
- UniSTS:245930

Mbp (e-PCR), detects polymorphism
- UniSTS:259500

D18Chm77 (e-PCR)
- UniSTS:518067

D18Chm78 (e-PCR)
- UniSTS:518090

BE102437 (e-PCR)
- UniSTS:244188

Mbp (e-PCR), detects polymorphism
- UniSTS:495847

D18Chm80 (e-PCR)
- UniSTS:518121

Homology

Orthologs from Annotation Pipeline: 293 organisms have orthologs with human gene Mbp
Orthologs

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables calmodulin binding	ISO Inferred from Sequence Orthology more info
enables protease binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables structural constituent of myelin sheath	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables structural constituent of myelin sheath	IEA Inferred from Electronic Annotation more info
enables structural constituent of myelin sheath	IMP Inferred from Mutant Phenotype more info	PubMed
enables structural constituent of myelin sheath	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in MAPK cascade	IEA Inferred from Electronic Annotation more info
involved_in MAPK cascade	ISO Inferred from Sequence Orthology more info
involved_in aging	IEP Inferred from Expression Pattern more info	PubMed
involved_in maintenance of blood-brain barrier	IEA Inferred from Electronic Annotation more info
involved_in maintenance of blood-brain barrier	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within membrane organization	ISO Inferred from Sequence Orthology more info
involved_in myelination	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in myelination	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within myelination	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of heterotypic cell-cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of heterotypic cell-cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of chemokine (C-X-C motif) ligand 2 production	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of chemokine (C-X-C motif) ligand 2 production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interleukin-6 production	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of interleukin-6 production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of metalloendopeptidase activity	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of metalloendopeptidase activity	ISO Inferred from Sequence Orthology more info
involved_in response to fatty acid	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to mercury ion	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to progesterone	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to toxic substance	ISO Inferred from Sequence Orthology more info
involved_in response to tumor necrosis factor	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within sensory perception of sound	ISO Inferred from Sequence Orthology more info
involved_in substantia nigra development	ISO Inferred from Sequence Orthology more info	PubMed

Component	Evidence Code	Pubs
is_active_in cell periphery	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in cell periphery	ISO Inferred from Sequence Orthology more info
located_in cell projection	ISO Inferred from Sequence Orthology more info
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
is_active_in compact myelin	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in compact myelin	IDA Inferred from Direct Assay more info	PubMed
located_in compact myelin	ISO Inferred from Sequence Orthology more info
is_active_in internode region of axon	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in internode region of axon	ISO Inferred from Sequence Orthology more info
located_in myelin sheath	IDA Inferred from Direct Assay more info	PubMed
located_in myelin sheath	IEA Inferred from Electronic Annotation more info
located_in myelin sheath	ISO Inferred from Sequence Orthology more info
located_in myelin sheath	TAS Traceable Author Statement more info	PubMed
is_active_in neuronal cell body	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: myelin basic protein; Golli-Mbp

Names: MBP S; myelin basic protein Golli-mbp; myelin basic protein S

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001025289.1 → NP_001020460.1 Golli-Mbp isoform 1

See identical proteins and their annotated locations for NP_001020460.1

Status: REVIEWED

Description

Transcript Variant: This variant (6) contains 3 exons unique to Golli-Mbp and exon 1 of the classic Mbp. It encodes Golli-mbp isoform 1.

Source sequence(s)

BC084713

UniProtKB/TrEMBL

Q5XFW1

Related

ENSRNOP00000070157.2, ENSRNOT00000089879.2

Conserved Domains (1) summary

pfam01669
Location:149 → 195

Myelin_MBP; Myelin basic protein
NM_001025291.1 → NP_001020462.1 myelin basic protein isoform 1

See identical proteins and their annotated locations for NP_001020462.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longest of the classic Mbp isoforms (1).

Source sequence(s)

AF439750, AI145512, AJ132898, CB586909, M25889

UniProtKB/Swiss-Prot

P02688

UniProtKB/TrEMBL

I7FKL4

Conserved Domains (1) summary

pfam01669
Location:16 → 194

Myelin_MBP; Myelin basic protein
NM_001025292.1 → NP_001020463.1 myelin basic protein isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks exon 2 compared to transcript variant 1 and encodes one of the classic Mbp isoforms (2) that is missing a 26 aa segment compared to isoform 1.

Source sequence(s)

AF439750, AI145512, AJ132897, CB586909, M25889

UniProtKB/Swiss-Prot

P02688

Conserved Domains (1) summary

pfam01669
Location:16 → 168

Myelin_MBP; Myelin basic protein
NM_001025293.1 → NP_001020464.1 myelin basic protein isoform 3

Status: REVIEWED

Description

Transcript Variant: This variant (3) lacks exon 6 compared to transcript variant 1 and encodes one of the classic Mbp isoforms (3) that is missing a 41 aa segment compared to isoform 1.

Source sequence(s)

AF439750, AI145512, AJ132896, CB586909, M25889

UniProtKB/Swiss-Prot

P02688

Conserved Domains (1) summary

pfam01669
Location:16 → 148

Myelin_MBP; Myelin basic protein
NM_001025294.1 → NP_001020465.1 myelin basic protein isoform 4

See identical proteins and their annotated locations for NP_001020465.1

Status: REVIEWED

Description

Transcript Variant: This variant (4) lacks exons 2 and 5 compared to transcript variant 1 and encodes one of the classic Mbp isoforms (4) that is missing a 26 aa and a 11 aa segment compared to isoform 1.

Source sequence(s)

AF439750, AI145512, CB586909, M25889

UniProtKB/Swiss-Prot

P02688

Conserved Domains (1) summary

pfam01669
Location:16 → 157

Myelin_MBP; Myelin basic protein
NM_017026.2 → NP_058722.1 myelin basic protein isoform 5

See identical proteins and their annotated locations for NP_058722.1

Status: REVIEWED

Description

Transcript Variant: This variant (5) lacks exons 2 and 6 compared to transcript variant 1 and encodes one of the classic Mbp isoforms (5) that is missing a 26 aa and a 41 aa segment compared to isoform 1.

Source sequence(s)

AI145512, BC094522, CB586909

UniProtKB/Swiss-Prot

P02688

Conserved Domains (1) summary

pfam01669
Location:16 → 122

Myelin_MBP; Myelin basic protein