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Ano2 anoctamin 2 [ Mus musculus (house mouse) ]

Gene ID: 243634, updated on 17-Aug-2024

Summary

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Official Symbol
Ano2provided by MGI
Official Full Name
anoctamin 2provided by MGI
Primary source
MGI:MGI:2387214
See related
Ensembl:ENSMUSG00000038115 AllianceGenome:MGI:2387214
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Tmem16b
Summary
Enables identical protein binding activity and intracellular calcium activated chloride channel activity. Acts upstream of or within chloride transport. Located in non-motile cilium and plasma membrane. Is expressed in dorsal root ganglion; epidermis; medulla oblongata basal plate mantle layer; olfactory epithelium; and spinal cord mantle layer. Orthologous to human ANO2 (anoctamin 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in testis adult (RPKM 1.4), placenta adult (RPKM 0.4) and 8 other tissues See more
Orthologs
human all
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Genomic context

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Location:
6 F3; 6 59.58 cM
Exon count:
28
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (125667382..126017115)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (125690419..126040133)

Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E2804 Neighboring gene STARR-seq mESC enhancer starr_17384 Neighboring gene predicted gene, 33144 Neighboring gene Von Willebrand factor Neighboring gene predicted gene, 38898 Neighboring gene predicted gene, 33202 Neighboring gene predicted gene, 24947 Neighboring gene STARR-seq mESC enhancer starr_17386 Neighboring gene STARR-seq mESC enhancer starr_17388 Neighboring gene STARR-seq mESC enhancer starr_17389 Neighboring gene neurotrophin 3 Neighboring gene STARR-seq mESC enhancer starr_17391 Neighboring gene STARR-seq mESC enhancer starr_17392 Neighboring gene predicted gene 10415

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Paradoxical electro-olfactogram responses in TMEM16B knock-out mice. Guarneri G, et al. Chem Senses, 2023 Jan 1. PMID 36744918, Free PMC Article
  2. TMEM16B Calcium-Activated Chloride Channels Regulate Action Potential Firing in Lateral Septum and Aggression in Male Mice. Wang L, et al. J Neurosci, 2019 Sep 4. PMID 31320449, Free PMC Article
  3. Calcium-activated chloride channels clamp odor-evoked spike activity in olfactory receptor neurons. Zak JD, et al. Sci Rep, 2018 Jul 13. PMID 30006552, Free PMC Article
  4. Ca(2+)-activated Cl current predominates in threshold response of mouse olfactory receptor neurons. Li RC, et al. Proc Natl Acad Sci U S A, 2018 May 22. PMID 29735665, Free PMC Article
  5. Inferior Olivary TMEM16B Mediates Cerebellar Motor Learning. Zhang Y, et al. Neuron, 2017 Aug 30. PMID 28858616, Free PMC Article

See all (52) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Paradoxical electro-olfactogram responses in TMEM16B knock-out mice.
    Title: Paradoxical electro-olfactogram responses in TMEM16B knock-out mice.
  2. TMEM16b regulates action potential firing in lateral septum and aggression in male mice.
    Title: TMEM16B Calcium-Activated Chloride Channels Regulate Action Potential Firing in Lateral Septum and Aggression in Male Mice.
  3. Ano2/TMEM16B is a Ca2+-activated chloride channel in vagal afferents of nodose neurons and a major determinant of CCK-induced satiety, body weight control, and energy expenditure, making it a potential therapeutic target in obesity.
    Title: TMEM16B determines cholecystokinin sensitivity of intestinal vagal afferents of nodose neurons.
  4. Here the authors report that Ano2 knockout mice exhibit impaired anxiety-related behaviors and context-independent fear memory, thus implicating TMEM16B in anxiety modulation.
    Title: TMEM16B regulates anxiety-related behavior and GABAergic neuronal signaling in the central lateral amygdala.
  5. Study provides the first in vivo evidence toward an alternative or additional role for Ano2 in the olfactory transduction cascade, where it may serve as a feedback mechanism to clamp olfactory receptor neurons spike output.
    Title: Calcium-activated chloride channels clamp odor-evoked spike activity in olfactory receptor neurons.
  6. Results of behavioral studies obtained from Ano2 (-/-) mice, a mouse line that was previously shown to lack this particular mode of ionic plasticity. The animals display motor coordination deficits that constitute a condition of mild ataxia. Moreover, motor learning is severely impaired in Ano2 (-/-) mice, suggesting cerebellar dysfunction.
    Title: Impaired Motor Coordination and Learning in Mice Lacking Anoctamin 2 Calcium-Gated Chloride Channels.
  7. Results suggest that the anoctamin 2-mediated amplification mechanism enables mice to track weak, unfamiliar olfactory cues.
    Title: Tracking of unfamiliar odors is facilitated by signal amplification through anoctamin 2 chloride channels in mouse olfactory receptor neurons.
  8. TMEM16B controls AP firing and ensures correct glomerular targeting of olfactory sensory neurons s expressing I7.
    Title: The Ca2+-activated Cl- channel TMEM16B regulates action potential firing and axonal targeting in olfactory sensory neurons.
  9. Loss of TMEM16B expression resulted in the absence of Calcium-activated Chloride channels in inferior Olive neurons, leading to markedly diminished action potential firing of IO neurons. TMEM16B knockout mice. Moreover, exhibited severe cerebellar motor learning deficits.
    Title: Inferior Olivary TMEM16B Mediates Cerebellar Motor Learning.
  10. The siRNA knock-down suggests that Ano2 contributes to Ca(2+)-dependent chloride conductance in the RPE.
    Title: Anoctamin2 (TMEM16B) forms the Ca(2+)-activated Cl(-) channel in the retinal pigment epithelium.
Submit: New GeneRIF Correction See all GeneRIFs (26)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC38715

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables chloride channel activity IEA
Inferred from Electronic Annotation
more info
  
enables chloride channel activity TAS
Traceable Author Statement
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables intracellularly calcium-gated chloride channel activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables intracellularly calcium-gated chloride channel activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables intracellularly calcium-gated chloride channel activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables intracellularly calcium-gated chloride channel activity ISO
Inferred from Sequence Orthology
more info
  
NOT enables phospholipid scramblase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein dimerization activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in chloride transmembrane transport IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within chloride transport IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within chloride transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within establishment of localization in cell IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within establishment of localization in cell IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within monoatomic ion transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within monoatomic ion transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of chloride channel complex IEA
Inferred from Electronic Annotation
more info
  
is_active_in glutamatergic synapse IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in neuron projection IDA
Inferred from Direct Assay
more info
 PubMed 
located_in non-motile cilium IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane ISO
Inferred from Sequence Orthology
more info
  
located_in plasma membrane TAS
Traceable Author Statement
more info
  
is_active_in presynaptic membrane IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
anoctamin-2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001364562.1 → NP_001351491.1  anoctamin-2 isoform 2

    Status: VALIDATED

    Source sequence(s)
    KC164759
    UniProtKB/TrEMBL
    M1GTU4, M1H0B0
    Conserved Domains (2) summary
    pfam04547
    Location:351 → 936
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:165 → 348
    Anoct_dimer; Dimerisation domain of Ca+-activated chloride-channel, anoctamin

  2. NM_001364563.1 → NP_001351492.1  anoctamin-2 isoform 3

    Status: VALIDATED

    Source sequence(s)
    KC164761
    UniProtKB/TrEMBL
    M1GNA0, M1GRW0
    Conserved Domains (2) summary
    pfam04547
    Location:289 → 874
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:103 → 286
    Anoct_dimer; Dimerisation domain of Ca+-activated chloride-channel, anoctamin

  3. NM_153589.2 → NP_705817.2  anoctamin-2 isoform 1

    See identical proteins and their annotated locations for NP_705817.2

    Status: VALIDATED

    Source sequence(s)
    AK044763, BC033409, BC060368
    Consensus CDS
    CCDS39643.2
    UniProtKB/Swiss-Prot
    C4N788, Q8C8N6, Q8CFW1
    UniProtKB/TrEMBL
    M1GTU4
    Related
    ENSMUSP00000125303.2, ENSMUST00000160496.8
    Conserved Domains (2) summary
    pfam04547
    Location:351 → 940
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:91 → 348
    Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000072.7 Reference GRCm39 C57BL/6J

    Range
    125667382..126017115
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006506117.4 → XP_006506180.1  anoctamin-2 isoform X2

    UniProtKB/TrEMBL
    M1GNA0
    Conserved Domains (2) summary
    pfam04547
    Location:289 → 878
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:29 → 286
    Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

  2. XM_006506115.3 → XP_006506178.1  anoctamin-2 isoform X1

    UniProtKB/TrEMBL
    M1GNA0
    Conserved Domains (2) summary
    pfam04547
    Location:306 → 895
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:46 → 303
    Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

  3. XM_006506119.3 → XP_006506182.1  anoctamin-2 isoform X3

    See identical proteins and their annotated locations for XP_006506182.1

    UniProtKB/TrEMBL
    M1GNA0
    Conserved Domains (2) summary
    pfam04547
    Location:262 → 851
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:2 → 259
    Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8CFW1.2 GenPept UniProtKB/Swiss-Prot:Q8CFW1

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