Osbpl8 oxysterol binding protein-like 8 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Osbpl8provided by MGI
Official Full Name: oxysterol binding protein-like 8provided by MGI
Primary source: MGI:MGI:2443807
See related: Ensembl:ENSMUSG00000020189 AllianceGenome:MGI:2443807
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: ORP-8; C730029P18Rik; D330025H14Rik
Summary: This gene encodes a member of the oxysterol-binding protein (Osbp) family, a group of intracellular lipid receptors. Like most members, the encoded protein contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal Osbp-like sterol-binding domain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Expression: Ubiquitous expression in CNS E18 (RPKM 7.5), frontal lobe adult (RPKM 6.6) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10; 10 D1

Exon count:: 26

Annotation release	Status	Assembly	Chr	Location
109	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (111000663..111133110)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (111164742..111297249)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Orp8 deficiency in bone marrow-derived cells reduces atherosclerotic lesion progression in LDL receptor knockout mice.
Title: Orp8 deficiency in bone marrow-derived cells reduces atherosclerotic lesion progression in LDL receptor knockout mice.
Osbpl8 deficiency in mouse causes an elevation of high-density lipoproteins and gender-specific alterations of lipid metabolism.
Title: Osbpl8 deficiency in mouse causes an elevation of high-density lipoproteins and gender-specific alterations of lipid metabolism.
aspects of ORP8 function in macrophages not directly involving lipid metabolism, but rather associated with nuclear functions, microtubule organization, and migration capacity
Title: Silencing of OSBP-related protein 8 (ORP8) modifies the macrophage transcriptome, nucleoporin p62 distribution, and migration capacity.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Osbpl8 (e-PCR), detects polymorphism
- UniSTS:265476

RH124508 (e-PCR)
- UniSTS:161330

D10Mit143 (e-PCR), detects polymorphism
- UniSTS:126044

RH125591 (e-PCR)
- UniSTS:162405

D10Ertd709e (e-PCR), detects polymorphism
- UniSTS:167031

Homology

Orthologs from Annotation Pipeline: 495 organisms have orthologs with human gene Osbpl8
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables cholesterol binding	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables cholesterol binding	ISO Inferred from Sequence Orthology more info
enables lipid binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol-4-phosphate binding	ISO Inferred from Sequence Orthology more info
enables phosphatidylserine binding	ISO Inferred from Sequence Orthology more info
enables phosphatidylserine transfer activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sterol transporter activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed

Process	Evidence Code	Pubs
involved_in activation of protein kinase B activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fat cell differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within lipid transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of sequestering of triglyceride	ISO Inferred from Sequence Orthology more info
involved_in phospholipid transport	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of glucose import	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of insulin receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of insulin receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of protein kinase B signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein kinase B signaling	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein localization to nuclear pore	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cortical endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
is_active_in intracellular membrane-bounded organelle	IBA Inferred from Biological aspect of Ancestor more info	PubMed
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in nuclear membrane	IMP Inferred from Mutant Phenotype more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: oxysterol-binding protein-related protein 8

Names: OSBP-related protein 8

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001003717.1 → NP_001003717.1 oxysterol-binding protein-related protein 8 isoform b

See identical proteins and their annotated locations for NP_001003717.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an alternate exon compared to variant 1, resulting in the use of a downstream in-frame start codon compared to variant 1. Variant 2 encodes isoform b, which has a shorter N-terminus compared to isoform a.

Source sequence(s)

AA536976, AK037869, AK050244, AK052311, BU152326, CF745582

Consensus CDS

CCDS24166.1

UniProtKB/Swiss-Prot

B9EJ86

UniProtKB/TrEMBL

A0A0R4J150

Related

ENSMUSP00000092948.2, ENSMUST00000095310.3

Conserved Domains (2) summary

cd13286
Location:100 → 229

PH_OPR5_ORP8; Human Oxysterol binding protein related proteins 5 and 8 Pleckstrin homology (PH) domain

pfam01237
Location:365 → 702

Oxysterol_BP; Oxysterol-binding protein
NM_175489.3 → NP_780698.2 oxysterol-binding protein-related protein 8 isoform a

See identical proteins and their annotated locations for NP_780698.2

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).

Source sequence(s)

AA536976, AK037158, AK037869, AK050244, AK052311, BU152326, CF745582

Consensus CDS

CCDS36056.1

UniProtKB/Swiss-Prot

B9EJ86, Q69ZJ4

Related

ENSMUSP00000100911.2, ENSMUST00000105275.9

Conserved Domains (3) summary

cd13286
Location:142 → 271

PH_OPR5_ORP8; Human Oxysterol binding protein related proteins 5 and 8 Pleckstrin homology (PH) domain

pfam00169
Location:149 → 265

PH; PH domain

pfam01237
Location:407 → 745

Oxysterol_BP; Oxysterol-binding protein

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

111000663..111133110

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036155782.1 → XP_036011675.1 oxysterol-binding protein-related protein 8 isoform X3

UniProtKB/TrEMBL

A0A0R4J150

Conserved Domains (2) summary

cd13286
Location:100 → 229

PH_OPR5_ORP8; Human Oxysterol binding protein related proteins 5 and 8 Pleckstrin homology (PH) domain

pfam01237
Location:365 → 702

Oxysterol_BP; Oxysterol-binding protein
XM_006513637.4 → XP_006513700.1 oxysterol-binding protein-related protein 8 isoform X1

Conserved Domains (2) summary

cd13286
Location:139 → 268

PH_OPR5_ORP8; Human Oxysterol binding protein related proteins 5 and 8 Pleckstrin homology (PH) domain

pfam01237
Location:404 → 741

Oxysterol_BP; Oxysterol-binding protein
XM_006513638.2 → XP_006513701.1 oxysterol-binding protein-related protein 8 isoform X2

See identical proteins and their annotated locations for XP_006513701.1

Related

ENSMUSP00000158904.2, ENSMUST00000238838.2

Conserved Domains (2) summary

cd13286
Location:118 → 247

PH_OPR5_ORP8; Human Oxysterol binding protein related proteins 5 and 8 Pleckstrin homology (PH) domain

pfam01237
Location:383 → 720

Oxysterol_BP; Oxysterol-binding protein