Dgkd diacylglycerol kinase, delta [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dgkdprovided by MGI
Official Full Name: diacylglycerol kinase, deltaprovided by MGI
Primary source: MGI:MGI:2138334
See related: Ensembl:ENSMUSG00000070738 AllianceGenome:MGI:2138334
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: dgkd-2; DGKdelta; D330025K09
Summary: Enables diacylglycerol kinase activity. Involved in diacylglycerol metabolic process; lipid phosphorylation; and regulation of signal transduction. Predicted to be located in clathrin-coated pit; cytoplasmic vesicle; and cytosol. Is expressed in several structures, including brain; immune system; musculature; reproductive system; and stomach. Used to study Lennox-Gastaut syndrome. Orthologous to human DGKD (diacylglycerol kinase delta). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in thymus adult (RPKM 36.4), spleen adult (RPKM 18.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1; 1 D

Exon count:: 34

Annotation release	Status	Assembly	Chr	Location
109	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (87780942..87872900)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (87853264..87945177)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

SFA/MUFA-PAs produced by DGKdelta are novel regulators of CKM function.
Title: Creatine kinase muscle type specifically interacts with saturated fatty acid- and/or monounsaturated fatty acid-containing phosphatidic acids.
myristic acid attenuates DGKdelta protein degradation in skeletal muscle cells and this attenuation is fatty acid-, protein- and cell line-specific.
Title: Myristic acid specifically stabilizes diacylglycerol kinase δ protein in C2C12 skeletal muscle cells.
The authors show that IFT88 interacts with DGKdelta, and is associated with COPII-coated vesicles at the the endoplasmic reticulum exit sites.
Title: DGKδ triggers endoplasmic reticulum release of IFT88-containing vesicles destined for the assembly of primary cilia.
results suggest that DGKdelta controls the down-regulation of cyclin D1 expression by attenuating the PKC signaling pathway for C2C12 myogenic differentiation
Title: Diacylglycerol kinase δ controls down-regulation of cyclin D1 for C2C12 myogenic differentiation.
the amount of 5-HT in the cerebral cortex was significantly decreased in DGKdelta-KO mice. These data strongly suggest that OCD-like behaviors in the DGKdelta-KO mice are caused by comprehensive and composite serotonergic hypofunction.
Title: Abnormalities of the serotonergic system in diacylglycerol kinase δ-deficient mouse brain.
Results strongly suggest that a deficiency of Diacylglycerol kinase delta induces obsessive-compulsive disorder-like behavior through enhancing axon/neurite outgrowth in the brain.
Title: Behavioral and pharmacological phenotypes of brain-specific diacylglycerol kinase δ-knockout mice.
DGKdelta deficiency impairs AMPK signaling and lipid metabolism, thereby highlighting the deleterious role of excessive lipid metabolites in the development of peripheral insulin resistance and type 2 diabetes pathogenesis.
Title: Diacylglycerol kinase-δ regulates AMPK signaling, lipid metabolism, and skeletal muscle energetics.
DGKD preferably metabolizes palmitic acid-containing diacylglycerol species supplied from the PC-PLC pathway.
Title: Diacylglycerol kinase δ phosphorylates phosphatidylcholine-specific phospholipase C-dependent, palmitic acid-containing diacylglycerol species in response to high glucose levels.
DGKdelta markedly increases lipid synthesis, at least in part as a result of promoting the synthesis of fatty acids.
Title: Diacylglycerol kinase delta promotes lipogenesis.
These results suggest that DGKzeta is involved in IQGAP1/Rac1-mediated phagocytosis upon LPS stimulation in macrophages.
Title: DGKζ is involved in LPS-activated phagocytosis through IQGAP1/Rac1 pathway.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (2) 1 citation
Targeted (1) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

UniSTS:498517 (e-PCR)
- UniSTS:498517

Dgkd (e-PCR), detects polymorphism
- UniSTS:498517

AI841987 (e-PCR)
- UniSTS:165086

Homology

Homologs of the Dgkd gene: The Dgkd gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, and frog.
Orthologs from Annotation Pipeline: 347 organisms have orthologs with human gene Dgkd
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables NAD+ kinase activity	IEA Inferred from Electronic Annotation more info
enables diacylglycerol kinase activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables diacylglycerol kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables diacylglycerol kinase activity	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables kinase binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein heterodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in diacylglycerol metabolic process	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in diacylglycerol metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within diacylglycerol metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within endocytosis	IEA Inferred from Electronic Annotation more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info	PubMed
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in lipid phosphorylation	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in lipid phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein kinase C signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphatidic acid biosynthetic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of clathrin-dependent endocytosis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of epidermal growth factor receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein kinase C-activating G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein transport	IEA Inferred from Electronic Annotation more info
involved_in response to organic substance	ISO Inferred from Sequence Orthology more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in clathrin-coated pit	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic vesicle	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: diacylglycerol kinase delta

Names: DAG kinase delta

NP_808314.2

EC 2.7.1.107

XP_006529534.1

EC 2.7.1.107

XP_006529535.1

EC 2.7.1.107

XP_011246290.1

EC 2.7.1.107

XP_017175841.1

EC 2.7.1.107

XP_030109593.1

EC 2.7.1.107

XP_036020405.1

EC 2.7.1.107

XP_036020407.1

EC 2.7.1.107

XP_036020416.1

EC 2.7.1.107

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_177646.3 → NP_808314.2 diacylglycerol kinase delta

Status: VALIDATED

Source sequence(s)

AC102630, AC162281

Consensus CDS

CCDS48312.1

UniProtKB/Swiss-Prot

E9PUQ8

UniProtKB/TrEMBL

E9PUQ8

Related

ENSMUSP00000027517.8, ENSMUST00000027517.14

Conserved Domains (6) summary

cd09575
Location:1147 → 1211

SAM_DGK-delta; SAM domain of diacylglycerol kinase delta

smart00045
Location:765 → 922

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:323 → 445

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd13274
Location:55 → 151

PH_DGK_type2; Type 2 Diacylglycerol kinase Pleckstrin homology (PH) domain

cd20847
Location:140 → 224

C1_DGKdelta_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase delta (DAG kinase delta) and similar proteins

cd20893
Location:231 → 291

C1_DGKdelta_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase delta (DAG kinase delta) and similar proteins

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

87780942..87872900

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006529472.5 → XP_006529535.1 diacylglycerol kinase delta isoform X2

Conserved Domains (4) summary

smart00045
Location:765 → 922

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:323 → 445

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:164 → 213

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd13274
Location:55 → 151

PH_DGK_type2; Type 2 Diacylglycerol kinase Pleckstrin homology (PH) domain
XM_036164514.1 → XP_036020407.1 diacylglycerol kinase delta isoform X4

Conserved Domains (6) summary

cd09575
Location:1109 → 1173

SAM_DGK-delta; SAM domain of diacylglycerol kinase delta

smart00046
Location:323 → 445

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd13274
Location:55 → 151

PH_DGK_type2; Type 2 Diacylglycerol kinase Pleckstrin homology (PH) domain

cd20847
Location:140 → 224

C1_DGKdelta_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase delta (DAG kinase delta) and similar proteins

cd20893
Location:231 → 291

C1_DGKdelta_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase delta (DAG kinase delta) and similar proteins

cl02440
Location:765 → 884

DAGK_acc; Diacylglycerol kinase accessory domain
XM_006529471.4 → XP_006529534.1 diacylglycerol kinase delta isoform X1

Conserved Domains (5) summary

smart00045
Location:765 → 922

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:323 → 445

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:164 → 213

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd13274
Location:55 → 151

PH_DGK_type2; Type 2 Diacylglycerol kinase Pleckstrin homology (PH) domain

cl15755
Location:1147 → 1193

SAM_superfamily; SAM (Sterile alpha motif )
XM_036164512.1 → XP_036020405.1 diacylglycerol kinase delta isoform X3

Conserved Domains (6) summary

smart00046
Location:323 → 445

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd13274
Location:55 → 151

PH_DGK_type2; Type 2 Diacylglycerol kinase Pleckstrin homology (PH) domain

cd20847
Location:140 → 224

C1_DGKdelta_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase delta (DAG kinase delta) and similar proteins

cd20893
Location:231 → 291

C1_DGKdelta_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase delta (DAG kinase delta) and similar proteins

cl02440
Location:765 → 884

DAGK_acc; Diacylglycerol kinase accessory domain

cl15755
Location:1109 → 1155

SAM_superfamily; SAM (Sterile alpha motif )
XM_030253733.2 → XP_030109593.1 diacylglycerol kinase delta isoform X5

Conserved Domains (5) summary

smart00045
Location:632 → 789

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:190 → 312

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:31 → 80

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cl15755
Location:1014 → 1060

SAM_superfamily; SAM (Sterile alpha motif )

cl17171
Location:1 → 18

PH-like; Pleckstrin homology-like domain
XM_036164523.1 → XP_036020416.1 diacylglycerol kinase delta isoform X6

Conserved Domains (5) summary

cd09575
Location:1014 → 1078

SAM_DGK-delta; SAM domain of diacylglycerol kinase delta

smart00045
Location:632 → 789

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:190 → 312

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd20847
Location:7 → 91

C1_DGKdelta_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase delta (DAG kinase delta) and similar proteins

cd20893
Location:98 → 158

C1_DGKdelta_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase delta (DAG kinase delta) and similar proteins
XM_011247988.4 → XP_011246290.1 diacylglycerol kinase delta isoform X5

See identical proteins and their annotated locations for XP_011246290.1

Conserved Domains (5) summary

smart00045
Location:632 → 789

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:190 → 312

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:31 → 80

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cl15755
Location:1014 → 1060

SAM_superfamily; SAM (Sterile alpha motif )

cl17171
Location:1 → 18

PH-like; Pleckstrin homology-like domain
XM_017320352.3 → XP_017175841.1 diacylglycerol kinase delta isoform X5

Conserved Domains (5) summary

smart00045
Location:632 → 789

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:190 → 312

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:31 → 80

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cl15755
Location:1014 → 1060

SAM_superfamily; SAM (Sterile alpha motif )

cl17171
Location:1 → 18

PH-like; Pleckstrin homology-like domain