Tpst2 protein-tyrosine sulfotransferase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tpst2provided by MGI
Official Full Name: protein-tyrosine sulfotransferase 2provided by MGI
Primary source: MGI:MGI:1309516
See related: Ensembl:ENSMUSG00000029344 AllianceGenome:MGI:1309516
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: grm; grt; D5Ucla3; Tango13b
Summary: Enables protein-tyrosine sulfotransferase activity. Acts upstream of or within fusion of sperm to egg plasma membrane involved in single fertilization; peptidyl-tyrosine sulfation; and prevention of polyspermy. Predicted to be located in endoplasmic reticulum. Predicted to be active in Golgi apparatus. Orthologous to human TPST2 (tyrosylprotein sulfotransferase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in genital fat pad adult (RPKM 79.3), adrenal adult (RPKM 73.7) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 F; 5 54.65 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
109	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (112424548..112463222)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (112276682..112315356)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

cerebral abnormalities in grt/grt Tpst2 gene defect mice are characterized by cortical hypoplasia at the frontooccipital axis with immature pyramidal neurons and insufficient development of callosal fibers
Title: Cerebral cortical hypoplasia with abnormal morphology of pyramidal neuron in growth-retarded mouse (grt/grt).
Findings indicate that low body weight and salivary gland hypofunction in Tpst2-/- mice is due solely to primary hypothyroidism.
Title: Salivary gland hypofunction in tyrosylprotein sulfotransferase-2 knockout mice is due to primary hypothyroidism.
Results demonstrate the importance of protein-tyrosine sulfation for proper development of the retina and suggest different phenotypes resulting from elimination of TPST-1 or -2 may reflect differential expression patterns or levels of the enzymes.
Title: Differential developmental deficits in retinal function in the absence of either protein tyrosine sulfotransferase-1 or -2.
Data show that Tpst1/Tpst2 DKO leukocytes bound less P-selectin than wild type leukocytes despite equivalent surface expression of Psgl-1.
Title: Tyrosine sulfation of native mouse Psgl-1 is required for optimal leukocyte rolling on P-selectin in vivo.
Lack of tyrosylprotein sulfotransferase-2 activity results in altered sperm-egg interactions and loss of ADAM3 and ADAM6 in epididymal sperm.
Title: Lack of tyrosylprotein sulfotransferase-2 activity results in altered sperm-egg interactions and loss of ADAM3 and ADAM6 in epididymal sperm.
These results indicate that protein-tyrosine sulfation by Tpst1/2 is essential for proper outer segment morphogenesis and synaptic function, but is not critical for overall retinal structure or synapse formation.
Title: Lack of protein-tyrosine sulfation disrupts photoreceptor outer segment morphogenesis, retinal function and retinal anatomy.
demonstrated that the Tpst2 transgene rescues the mutant phenotypes both in vitro and in vivo
Title: Hypothyroid phenotype of the Tpst2 mutant mouse is dependent upon genetic background.
Considering that Tpst2 is the responsible gene of grt mice, mutation of which is associated with a poor function of TSH receptor, the findings raise the possibility of involvement of factors including Tpst2 in the insulin hyposecretion in grt mice.
Title: Impaired insulin secretion from the pancreatic islets of hypothyroidal growth-retarded mice.
demonstrate that RNase 9 and Mfge8 are tyrosine-sulfated in wild type and Tpst1(-/-), but not in Tpst2(-/-) mice. This suggests lack of sulfation of one or both of these proteins may contribute mechanistically to the infertility of Tpst2(-/-) males
Title: Tyrosylprotein sulfotransferase-2 expression is required for sulfation of RNase 9 and Mfge8 in vivo.
Tpst1 and Tpst2 are the only Tpst genes in mice, tyrosine sulfation is required for normal pulmonary function at birth, and TPST-2 is required for normal thyroid gland function.
Title: Early postnatal pulmonary failure and primary hypothyroidism in mice with combined TPST-1 and TPST-2 deficiency.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Phenotypes

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Associated conditions

Description	Tests
growth retarded GeneReviews: Not available

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Spontaneous (1) 1 citation
Gene trapped (1)
Targeted (1) 1 citation
Endonuclease-mediated (1)

General gene information

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Markers

AI448750 (e-PCR)
- UniSTS:165419

37.MMHAP46FRE10.seq (e-PCR)
- UniSTS:124233

D5Mit94 (e-PCR), detects polymorphism
- UniSTS:130636

D5Mit94.1 (e-PCR), detects polymorphism
- UniSTS:130637

D5Mit26 (e-PCR), detects polymorphism
- UniSTS:130395

D1Bda11 (e-PCR)
- UniSTS:239758

Homology

Homologs of the Tpst2 gene: The Tpst2 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, zebrafish, and frog.
Orthologs from Annotation Pipeline: 341 organisms have orthologs with human gene Tpst2
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein-tyrosine sulfotransferase activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables protein-tyrosine sulfotransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein-tyrosine sulfotransferase activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within fusion of sperm to egg plasma membrane involved in single fertilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptidyl-tyrosine sulfation	IBA Inferred from Biological aspect of Ancestor more info	PubMed
acts_upstream_of_or_within peptidyl-tyrosine sulfation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptidyl-tyrosine sulfation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within prevention of polyspermy	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein-tyrosine sulfotransferase 2

Names: TPST-2; tyrosylprotein sulfotransferase 2

NP_001313519.2

EC 2.8.2.20

NP_001361637.1

EC 2.8.2.20

NP_001361638.1

EC 2.8.2.20

NP_033445.3

EC 2.8.2.20

XP_006534912.1

EC 2.8.2.20

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001326590.2 → NP_001313519.2 protein-tyrosine sulfotransferase 2 precursor

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 4. Variants 1-4 all encode the same protein.

Source sequence(s)

AC123795, AC123848

UniProtKB/Swiss-Prot

Q8CBA8

Related

ENSMUSP00000071399.3, ENSMUST00000071455.7

Conserved Domains (1) summary

pfam13469
Location:70 → 261

Sulfotransfer_3; Sulfotransferase family
NM_001374708.1 → NP_001361637.1 protein-tyrosine sulfotransferase 2 precursor

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 4. Variants 1-4 all encode the same protein.

Source sequence(s)

AC123848

UniProtKB/Swiss-Prot

Q8CBA8

Conserved Domains (1) summary

pfam13469
Location:70 → 261

Sulfotransfer_3; Sulfotransferase family
NM_001374709.1 → NP_001361638.1 protein-tyrosine sulfotransferase 2 precursor

Status: VALIDATED

Description

Transcript Variant: This variant (4) represents the longest transcript. Variants 1-4 all encode the same protein.

Source sequence(s)

AC123848

UniProtKB/Swiss-Prot

Q8CBA8

Conserved Domains (1) summary

pfam13469
Location:70 → 261

Sulfotransfer_3; Sulfotransferase family
NM_009419.5 → NP_033445.3 protein-tyrosine sulfotransferase 2 precursor

Status: VALIDATED

Description

Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 4. Variants 1-4 all encode the same protein.

Source sequence(s)

AC123795, AC123848

UniProtKB/Swiss-Prot

Q8CBA8

Related

ENSMUSP00000031287.6, ENSMUST00000031287.12

Conserved Domains (1) summary

pfam13469
Location:70 → 261

Sulfotransfer_3; Sulfotransferase family

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

112424548..112463222

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006534849.5 → XP_006534912.1 protein-tyrosine sulfotransferase 2 isoform X1

Conserved Domains (1) summary

pfam13469
Location:135 → 326

Sulfotransfer_3; Sulfotransferase family