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EP300 E1A binding protein p300 [ Homo sapiens (human) ]

Gene ID: 2033, updated on 29-Mar-2020

Summary

Official Symbol
EP300provided by HGNC
Official Full Name
E1A binding protein p300provided by HGNC
Primary source
HGNC:HGNC:3373
See related
Ensembl:ENSG00000100393 MIM:602700
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
p300; KAT3B; MKHK2; RSTS2
Summary
This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in bone marrow (RPKM 27.3), testis (RPKM 17.5) and 25 other tissues See more
Orthologs

Genomic context

See EP300 in Genome Data Viewer
Location:
22q13.2
Exon count:
31
Annotation release Status Assembly Chr Location
109.20200228 current GRCh38.p13 (GCF_000001405.39) 22 NC_000022.11 (41092592..41180077)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 22 NC_000022.10 (41488614..41576081)

Chromosome 22 - NC_000022.11Genomic Context describing neighboring genes Neighboring gene actin gamma 2, smooth muscle pseudogene Neighboring gene microRNA 1281 Neighboring gene RNA, U6 small nuclear 375, pseudogene Neighboring gene EP300 antisense RNA 1 Neighboring gene leucine rich repeat containing 37 member A14, pseudogene Neighboring gene uncharacterized LOC646927 Neighboring gene L3MBTL2 antisense RNA 1 Neighboring gene L3MBTL histone methyl-lysine binding protein 2

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phenotypes

Copy number response

Description
Copy number response
Triplosensitivity

No evidence available (Last evaluated (2013-03-14)

ClinGen Genome Curation Page
Haploinsufficency

Sufficient evidence for dosage pathogenicity (Last evaluated (2013-03-14)

ClinGen Genome Curation PagePubMed

NHGRI GWAS Catalog

Description
A genome-wide association study on a southern European population identifies a new Crohn's disease susceptibility locus at RBX1-EP300.
NHGRI GWA Catalog
Biological insights from 108 schizophrenia-associated genetic loci.
NHGRI GWA Catalog

HIV-1 interactions

Replication interactions

Interaction Pubs
Knockdown of E1A binding protein p300 (EP300) by siRNA inhibits the early stages of HIV-1 replication in 293T cells infected with VSV-G pseudotyped HIV-1 PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 gp120 and p300 synergistically increase TGF-beta, ATF-2 and activating protein-1 (AP-1) expression leading to tubular cell apoptosis; in addition, HIV-1 gp120 treatment causes phosphorylation of Smad2 and downregulation of c-Jun PubMed
env HIV-1 gp120-induced apoptosis in human proximal renal tubular cells is significantly enhanced by p300, and Smad7 and an anti-TGF-beta antibody inhibit this effect of p300 PubMed
Tat tat HIV-1 Tat interaction with p300 and other histone acetyl transferases is preferentially important for Tat-mediated transactivation of integrated, but not unintegrated, HIV-1 LTR promoter PubMed
tat Acetylation of HIV-1 Tat by p300 at Lys50 regulates Tat binding to cyclin T1 and HIV-1 TAR RNA, as well as Tat-mediated transactivation of the HIV-1 LTR promoter, by allowing Tat to dissociate from TAR RNA PubMed
tat HIV-1 Tat is acetylated by p300 on Lys50 and Lys51 PubMed
tat HIV-1 Tat-induced upregulation and phosphorylation of STAT3 expression results in increased GFAP, Egr-1, and p300 transcription and protein expression in both Tat-expressing astrocytes, and HIV-infected astrocytes PubMed
tat HIV-1 Tat-induced upregulation of p300 occurs at the transcriptional level through the cis-acting elements of early growth response 1 (egr-1) within its promoter PubMed
tat HIV-1 Tat, NAP-1, and p300 synergistically activate HIV-1 transcription PubMed
tat Tat stimulates NF-kappaB-inducing kinase IKKalpha translocation from the cytoplasm to the nucleus in monocytes, which leads to IKKalpha and CBP/p300 recruitment to the IL-10 promoter and histone H3 phosphorylation (Ser 10) and acetylation (Lys 14) PubMed
tat HIV-1 Tat-mediated upregulation of adenovirus E1a-associated 300 kDa protein p300 regulates increased GFAP expression in astrocytes PubMed
tat HIV-1 Tat interaction with p300 increases the histone acetyl transferase (HAT) activity of p300 on histone H4, indicating Tat modulates HAT activity of co-activators to derepress the HIV-1 chromatin structure and aid in activated transcription PubMed
tat HIV-1 Tat forms a ternary complex with p300 and P/CAF PubMed
tat Binding of HIV-1 Tat to p300 induces a conformational change in the CBP/p300 complex such that it can acquire and bind better to basal transcription factors, indicating Tat helps CBP/p300 recruit new partners to the transcription machinery PubMed
tat HIV-1 Tat recruits CBP/p300 to the chromosomally integrated HIV-1 LTR promoter PubMed
tat Acetylation of the p50 subunit of NFkappaB by CBP/p300 is regulated by HIV-1 Tat PubMed
tat INI-1 and BRG-1 synergize with the p300 acetyltransferase to activate the HIV promoter; this synergism depends on the acetyltransferase activity of p300 and on HIV-1 Tat amino acids Lys(50) and Lys(51), suggesting a regulation of Tat by these proteins PubMed
tat HIV-1 Tat binds to the minimal histone acetyltransferase domain (amino acids 1253-1710) and E1a binding domain (amino acids 1542-1710) of p300, an effect mediated by the basic domain (amino acids 48-57) of Tat PubMed
tat HIV-1 Tat induces the polyubiquitination and degradation of Tip60 through p300/CBP-associated E4-type ubiquitin-ligase activity, which results in a dramatic impairment of the Tip60-dependent apoptotic cell response to DNA damage PubMed
Vpr vpr HIV-1 Vpr and p300/HAT colocalizes with CENP-A and CENP-H proteins in the centromere and arm region of chromosome PubMed
vpr HIV-1 Vpr actively recruits p300/HAT to a range of chromatine regions including the centromere, resulting in the displacement of HP1-alpha PubMed
vpr Data suggests that HIV-1 Vpr, Rel A, and p300 act in concert to activate HIV transcription PubMed
vpr Adenovirus E1A protein represses activation by Vpr by competing for binding to p300, suggesting that p300 is required for activation of HIV transcription by Vpr PubMed
vpr Transcription Factor IIH (TFIIH) and p300 act cooperatively to enhance Vpr effects on glucocorticoid receptor transactivation PubMed
vpr The C-terminus of HIV-1 Vpr (amino acids 64-84) mediates the binding of Vpr to p300 (amino acids 2045-2191) PubMed
vpr HIV-1 Vpr transactivation of HIV transcription and of the glucocorticoid receptor pathway is mediated by direct binding to the p300 co-activator PubMed
integrase gag-pol The interaction between HIV-1 IN and KAP1 is highly favored by HAT/p300-mediated IN acetylation PubMed
gag-pol HAT/p300 acetylates lysine's 264, 266, and 273 of HIV-1 IN PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
NF-kappaB binding IPI
Inferred from Physical Interaction
more info
PubMed 
RNA polymerase II activating transcription factor binding IPI
Inferred from Physical Interaction
more info
PubMed 
RNA polymerase II proximal promoter sequence-specific DNA binding IEA
Inferred from Electronic Annotation
more info
 
RNA polymerase II regulatory region sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
RNA polymerase II transcription factor binding IEA
Inferred from Electronic Annotation
more info
 
STAT family protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
acetyltransferase activity EXP
Inferred from Experiment
more info
PubMed 
acetyltransferase activity IDA
Inferred from Direct Assay
more info
PubMed 
acetyltransferase activity IMP
Inferred from Mutant Phenotype
more info
PubMed 
activating transcription factor binding IPI
Inferred from Physical Interaction
more info
PubMed 
androgen receptor binding IPI
Inferred from Physical Interaction
more info
PubMed 
beta-catenin binding IPI
Inferred from Physical Interaction
more info
PubMed 
chromatin DNA binding IEA
Inferred from Electronic Annotation
more info
 
chromatin binding IDA
Inferred from Direct Assay
more info
PubMed 
chromatin binding IMP
Inferred from Mutant Phenotype
more info
PubMed 
damaged DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
histone acetyltransferase activity IDA
Inferred from Direct Assay
more info
PubMed 
histone acetyltransferase activity TAS
Traceable Author Statement
more info
PubMed 
histone butyryltransferase activity IEA
Inferred from Electronic Annotation
more info
 
histone crotonyltransferase activity IDA
Inferred from Direct Assay
more info
PubMed 
lysine N-acetyltransferase activity, acting on acetyl phosphate as donor IDA
Inferred from Direct Assay
more info
PubMed 
nuclear hormone receptor binding IPI
Inferred from Physical Interaction
more info
PubMed 
p53 binding IEA
Inferred from Electronic Annotation
more info
 
peptide butyryltransferase activity IDA
Inferred from Direct Assay
more info
PubMed 
peptide-lysine-N-acetyltransferase activity TAS
Traceable Author Statement
more info
 
pre-mRNA intronic binding IEA
Inferred from Electronic Annotation
more info
 
protein C-terminus binding IDA
Inferred from Direct Assay
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein propionyltransferase activity IDA
Inferred from Direct Assay
more info
PubMed 
tau protein binding NAS
Non-traceable Author Statement
more info
PubMed 
transcription coactivator activity IDA
Inferred from Direct Assay
more info
PubMed 
transcription coactivator activity IMP
Inferred from Mutant Phenotype
more info
PubMed 
transcription coactivator activity IPI
Inferred from Physical Interaction
more info
PubMed 
transcription coactivator activity TAS
Traceable Author Statement
more info
PubMed 
transcription factor binding IPI
Inferred from Physical Interaction
more info
PubMed 
transferase activity, transferring acyl groups IDA
Inferred from Direct Assay
more info
PubMed 
zinc ion binding IEA
Inferred from Electronic Annotation
more info
 
Process Evidence Code Pubs
B cell differentiation IEA
Inferred from Electronic Annotation
more info
 
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest TAS
Traceable Author Statement
more info
 
N-terminal peptidyl-lysine acetylation IDA
Inferred from Direct Assay
more info
PubMed 
Notch signaling pathway TAS
Traceable Author Statement
more info
 
animal organ morphogenesis IEA
Inferred from Electronic Annotation
more info
 
apoptotic process IMP
Inferred from Mutant Phenotype
more info
PubMed 
behavioral defense response IEA
Inferred from Electronic Annotation
more info
 
beta-catenin-TCF complex assembly TAS
Traceable Author Statement
more info
 
cellular response to UV IDA
Inferred from Direct Assay
more info
PubMed 
circadian rhythm ISS
Inferred from Sequence or Structural Similarity
more info
 
face morphogenesis IEA
Inferred from Electronic Annotation
more info
 
fat cell differentiation ISS
Inferred from Sequence or Structural Similarity
more info
 
heart development IEA
Inferred from Electronic Annotation
more info
 
histone H2B acetylation IDA
Inferred from Direct Assay
more info
PubMed 
histone H4 acetylation IMP
Inferred from Mutant Phenotype
more info
PubMed 
histone acetylation IDA
Inferred from Direct Assay
more info
PubMed 
internal peptidyl-lysine acetylation IDA
Inferred from Direct Assay
more info
PubMed 
internal protein amino acid acetylation IDA
Inferred from Direct Assay
more info
PubMed 
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator IDA
Inferred from Direct Assay
more info
PubMed 
learning or memory IEA
Inferred from Electronic Annotation
more info
 
lung development IEA
Inferred from Electronic Annotation
more info
 
macrophage derived foam cell differentiation IDA
Inferred from Direct Assay
more info
PubMed 
megakaryocyte development IEA
Inferred from Electronic Annotation
more info
 
multicellular organism growth IEA
Inferred from Electronic Annotation
more info
 
negative regulation of gluconeogenesis IDA
Inferred from Direct Assay
more info
PubMed 
negative regulation of protein complex assembly IDA
Inferred from Direct Assay
more info
PubMed 
negative regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
PubMed 
nervous system development TAS
Traceable Author Statement
more info
PubMed 
peptidyl-lysine acetylation IDA
Inferred from Direct Assay
more info
PubMed 
peptidyl-lysine butyrylation IDA
Inferred from Direct Assay
more info
PubMed 
peptidyl-lysine crotonylation IDA
Inferred from Direct Assay
more info
PubMed 
peptidyl-lysine propionylation IDA
Inferred from Direct Assay
more info
PubMed 
platelet formation IEA
Inferred from Electronic Annotation
more info
 
positive regulation by host of viral transcription IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of DNA-binding transcription factor activity IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of NIK/NF-kappaB signaling IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of Notch signaling pathway TAS
Traceable Author Statement
more info
 
positive regulation of RNA polymerase II regulatory region sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of gene expression, epigenetic IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of gene expression, epigenetic TAS
Traceable Author Statement
more info
 
positive regulation of neuron projection development IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of protein binding IEA
Inferred from Electronic Annotation
more info
 
positive regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of transcription by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of transcription by RNA polymerase II TAS
Traceable Author Statement
more info
 
positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response ISS
Inferred from Sequence or Structural Similarity
more info
 
positive regulation of transcription of Notch receptor target TAS
Traceable Author Statement
more info
 
positive regulation of transcription, DNA-templated TAS
Traceable Author Statement
more info
PubMed 
positive regulation of transforming growth factor beta receptor signaling pathway IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of type I interferon production TAS
Traceable Author Statement
more info
 
protein acetylation IDA
Inferred from Direct Assay
more info
PubMed 
protein acetylation TAS
Traceable Author Statement
more info
PubMed 
protein destabilization IMP
Inferred from Mutant Phenotype
more info
PubMed 
protein deubiquitination TAS
Traceable Author Statement
more info
 
protein stabilization ISS
Inferred from Sequence or Structural Similarity
more info
 
regulation of androgen receptor signaling pathway IDA
Inferred from Direct Assay
more info
PubMed 
regulation of autophagy TAS
Traceable Author Statement
more info
PubMed 
regulation of cell cycle TAS
Traceable Author Statement
more info
 
regulation of cellular response to heat TAS
Traceable Author Statement
more info
 
regulation of glycolytic process IDA
Inferred from Direct Assay
more info
PubMed 
regulation of megakaryocyte differentiation TAS
Traceable Author Statement
more info
 
regulation of mitochondrion organization ISS
Inferred from Sequence or Structural Similarity
more info
 
regulation of signal transduction by p53 class mediator TAS
Traceable Author Statement
more info
 
regulation of transcription from RNA polymerase II promoter in response to hypoxia TAS
Traceable Author Statement
more info
 
regulation of transcription, DNA-templated IDA
Inferred from Direct Assay
more info
PubMed 
regulation of tubulin deacetylation IDA
Inferred from Direct Assay
more info
PubMed 
response to estrogen IDA
Inferred from Direct Assay
more info
PubMed 
response to hypoxia IDA
Inferred from Direct Assay
more info
PubMed 
skeletal muscle tissue development IEA
Inferred from Electronic Annotation
more info
 
somitogenesis IEA
Inferred from Electronic Annotation
more info
 
stimulatory C-type lectin receptor signaling pathway TAS
Traceable Author Statement
more info
 
swimming IEA
Inferred from Electronic Annotation
more info
 
thigmotaxis IEA
Inferred from Electronic Annotation
more info
 
transcription-coupled nucleotide-excision repair TAS
Traceable Author Statement
more info
 
viral process IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
chromosome IEA
Inferred from Electronic Annotation
more info
 
cytosol IDA
Inferred from Direct Assay
more info
 
histone acetyltransferase complex IEA
Inferred from Electronic Annotation
more info
 
host cell nucleus IEA
Inferred from Electronic Annotation
more info
 
nucleoplasm IDA
Inferred from Direct Assay
more info
 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 
protein-DNA complex ISS
Inferred from Sequence or Structural Similarity
more info
PubMed 
transcription factor complex IEA
Inferred from Electronic Annotation
more info
 

General protein information

Preferred Names
histone acetyltransferase p300
Names
E1A-associated protein p300
E1A-binding protein, 300kD
histone butyryltransferase p300
histone crotonyltransferase p300
p300 HAT
protein 2-hydroxyisobutyryltransferase p300
protein propionyltransferase p300
NP_001349772.1
NP_001420.2

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_009817.1 RefSeqGene

    Range
    4983..92468
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001362843.2NP_001349772.1  histone acetyltransferase p300 isoform 2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region, compared to variant 1, which results in a shorter protein (isoform 2) compared to isoform 1.
    Source sequence(s)
    AL035658, AL080243, AL096765
    Related
    ENSP00000501078.1, ENST00000674155.1
    Conserved Domains (11) summary
    cd05495
    Location:10251132
    Bromo_cbp_like; Bromodomain, cbp_like subfamily. Cbp (CREB binding protein or CREBBP) is an acetyltransferase acting on histone, which gives a specific tag for transcriptional activation and also acetylates non-histone proteins. CREBBP binds specifically to ...
    smart00551
    Location:17031781
    ZnF_TAZ; TAZ zinc finger, present in p300 and CBP
    cd02337
    Location:16421682
    ZZ_CBP; Zinc finger, ZZ type. Zinc finger present in CBP/p300 and related proteins. The ZZ motif coordinates two zinc ions and most likely participates in ligand binding or molecular scaffolding. CREB-binding protein (CBP) is a large multidomain protein that ...
    cd15802
    Location:11441216
    RING_CBP-p300; atypical RING domain found in CREB-binding protein and p300 histone acetyltransferases
    cd15646
    Location:12171251
    PHD_p300; PHD finger found in histone acetyltransferase p300
    pfam02172
    Location:566646
    KIX; KIX domain
    pfam02135
    Location:347414
    zf-TAZ; TAZ zinc finger
    pfam08214
    Location:12801586
    HAT_KAT11; Histone acetylation protein
    pfam09030
    Location:19792071
    Creb_binding; Creb binding
    pfam09606
    Location:19582382
    Med15; ARC105 or Med15 subunit of Mediator complex non-fungal
    cl25526
    Location:9351038
    TonB_N; TonB N-terminal region
  2. NM_001429.4NP_001420.2  histone acetyltransferase p300 isoform 1

    See identical proteins and their annotated locations for NP_001420.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longer isoform (1).
    Source sequence(s)
    AL035658, AL080243, BC053889, CN278277, CN278282, DB047746, N57495, U01877
    Consensus CDS
    CCDS14010.1
    UniProtKB/Swiss-Prot
    Q09472
    UniProtKB/TrEMBL
    Q7Z6C1
    Related
    ENSP00000263253.7, ENST00000263253.9
    Conserved Domains (11) summary
    cd05495
    Location:10511158
    Bromo_cbp_like; Bromodomain, cbp_like subfamily. Cbp (CREB binding protein or CREBBP) is an acetyltransferase acting on histone, which gives a specific tag for transcriptional activation and also acetylates non-histone proteins. CREBBP binds specifically to ...
    smart00551
    Location:17291807
    ZnF_TAZ; TAZ zinc finger, present in p300 and CBP
    cd02337
    Location:16681708
    ZZ_CBP; Zinc finger, ZZ type. Zinc finger present in CBP/p300 and related proteins. The ZZ motif coordinates two zinc ions and most likely participates in ligand binding or molecular scaffolding. CREB-binding protein (CBP) is a large multidomain protein that ...
    pfam08430
    Location:124258
    Forkhead_N; Forkhead N-terminal region
    pfam09606
    Location:19372412
    Med15; ARC105 or Med15 subunit of Mediator complex non-fungal
    cd15802
    Location:11701242
    RING_CBP-p300; atypical RING domain found in CREB-binding protein and p300 histone acetyltransferases
    cd15646
    Location:12431277
    PHD_p300; PHD finger found in histone acetyltransferase p300
    pfam02135
    Location:347414
    zf-TAZ; TAZ zinc finger
    pfam02172
    Location:566646
    KIX; KIX domain
    pfam08214
    Location:13061612
    HAT_KAT11; Histone acetylation protein
    pfam09030
    Location:20052095
    Creb_binding; Creb binding

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000022.11 Reference GRCh38.p13 Primary Assembly

    Range
    41092592..41180077
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
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