Rnf2 ring finger protein 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rnf2provided by MGI
Official Full Name: ring finger protein 2provided by MGI
Primary source: MGI:MGI:1101759
See related: Ensembl:ENSMUSG00000026484 AllianceGenome:MGI:1101759
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: dinG; Ring1B
Summary: Enables chromatin binding activity and ubiquitin protein ligase activity. Involved in germ cell development and histone H2A-K119 monoubiquitination. Acts upstream of or within with a negative effect on gene expression. Acts upstream of or within several processes, including gastrulation with mouth forming second; histone ubiquitination; and negative regulation of transcription by RNA polymerase II. Located in euchromatin; nuclear body; and sex chromatin. Part of PcG protein complex and ubiquitin ligase complex. Is expressed in several structures, including central nervous system; limb; ovary; retina; and urinary system. Orthologous to human RNF2 (ring finger protein 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in CNS E11.5 (RPKM 21.3), placenta adult (RPKM 21.2) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1; 1 G1

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
109	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (151345149..151376747, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (151469398..151500991, complement)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

RNF2 ablation reprograms the tumor-immune microenvironment and stimulates durable NK and CD4(+) T-cell-dependent antitumor immunity.
Title: RNF2 ablation reprograms the tumor-immune microenvironment and stimulates durable NK and CD4(+) T-cell-dependent antitumor immunity.
Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4.
Title: Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4.
Functional associations among DNA, transcription, and chromatin over PcG-repressed genes can be regulated by the formation of R-loops and that RING1B may play a vital role in this pathway for transcriptional repression.
Title: R-Loops Enhance Polycomb Repression at a Subset of Developmental Regulator Genes.
Ring1A/B regulate and maintain AML stem cells in part by repressing Glis2 expression, which promotes their differentiation.
Title: Ring1A and Ring1B inhibit expression of Glis2 to maintain murine MOZ-TIF2 AML stem cells.
Data show that RNF2 promotes STAT1/STAT2 disassociation from DNA.
Title: Nuclear RNF2 inhibits interferon function by promoting K33-linked STAT1 disassociation from DNA.
the ability of RING1B, a core component of PRC1, to ubiquitinate histone H2A is dispensable for early mouse embryonic development and much of the gene repression activity of PRC1
Title: The E3 ubiquitin ligase activity of RING1B is not essential for early mouse development.
RNF2 associates with AMBRA1 to act as an E3 ligase to ubiquitinate AMBRA1 via K48 linkage.
Title: RNF2 is recruited by WASH to ubiquitinate AMBRA1 leading to downregulation of autophagy.
The polycomb component Ring1B regulates the timed termination of Fezf2 expression and thus subcerebral projection neuron production during mouse neocortical development.
Title: The polycomb component Ring1B regulates the timed termination of subcerebral projection neuron production during mouse neocortical development.
Expansion of hepatic stem/progenitor cells requires Ring1B-mediated epigenetic silencing of Cdkn1a and Cdkn2a, demonstrating that Ring1B simultaneously regulates multiple CDKIs in tissue stem/progenitor cells.
Title: Ring1B promotes hepatic stem/progenitor cell expansion through simultaneous suppression of Cdkn1a and Cdkn2a in mice.
Af9 mediates site-selective physical and functional recruitment of Rnf2 to the alpha-ENaC promoter to constrain basal alpha-ENaC transcription in collecting duct cells.
Title: Physical and functional interaction of Rnf2 with Af9 regulates basal and aldosterone-stimulated transcription of the α-ENaC gene in a renal collecting duct cell line.

Submit: New GeneRIF Correction See all GeneRIFs (35)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (9) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI326319 (e-PCR)
- UniSTS:178477

RH136542 (e-PCR)
- UniSTS:210524

AI450156 (e-PCR)
- UniSTS:158866

Homology

Orthologs from Annotation Pipeline: 482 organisms have orthologs with human gene Rnf2
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables RING-like zinc finger domain binding	ISO Inferred from Sequence Orthology more info
enables chromatin binding	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin binding	ISO Inferred from Sequence Orthology more info
enables histone H2AK119 ubiquitin ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within anterior/posterior axis specification	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within_positive_effect chromatin organization	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within_positive_effect chromatin organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in chromatin remodeling	ISO Inferred from Sequence Orthology more info
involved_in epigenetic regulation of gene expression	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within gastrulation with mouth forming second	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within_negative_effect gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germ cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in histone H2A monoubiquitination	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in histone H2A-K119 monoubiquitination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of MLL1 complex	ISO Inferred from Sequence Orthology more info
part_of PRC1 complex	IDA Inferred from Direct Assay more info	PubMed
part_of PRC1 complex	ISO Inferred from Sequence Orthology more info
part_of PcG protein complex	IBA Inferred from Biological aspect of Ancestor more info	PubMed
part_of PcG protein complex	IDA Inferred from Direct Assay more info	PubMed
part_of PcG protein complex	ISO Inferred from Sequence Orthology more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in euchromatin	IDA Inferred from Direct Assay more info	PubMed
located_in heterochromatin	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear body	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear body	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in sex chromatin	IDA Inferred from Direct Assay more info	PubMed
part_of ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info	PubMed
part_of ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase RING2

Names: RING finger protein 1B; RING-type E3 ubiquitin transferase RING2

NP_001347773.1

EC 2.3.2.27

NP_001347774.1

EC 2.3.2.27

NP_001347776.1

EC 2.3.2.27

NP_035407.1

EC 2.3.2.27

XP_006529332.1

EC 2.3.2.27

XP_036018759.1

EC 2.3.2.27

XP_036018760.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001360844.1 → NP_001347773.1 E3 ubiquitin-protein ligase RING2 isoform a

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a).

Source sequence(s)

AC115060, AC165946

Conserved Domains (2) summary

cd16740
Location:56 → 102

RING-HC_RING2; RING finger, HC subclass, found in really interesting new gene 2 protein (RING2) and similar proteins

cd17167
Location:234 → 339

RAWUL_RING2; RING finger- and WD40-associated ubiquitin-like (RAWUL) domain found in really interesting new gene 2 protein (RING2)
NM_001360845.1 → NP_001347774.1 E3 ubiquitin-protein ligase RING2 isoform b

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform (b).

Source sequence(s)

AC115060, AC165946

Consensus CDS

CCDS15362.1

UniProtKB/Swiss-Prot

Q8C1X8

UniProtKB/TrEMBL

A0A6P5QDT0, A0A8C6GSC0

Conserved Domains (3) summary

COG5222
Location:11 → 103

COG5222; Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only]

cd16531
Location:51 → 91

RING-HC_RING1_like; RING finger, HC subclass, found in really interesting new gene proteins RING1, RING2 and similar proteins

cd17167
Location:225 → 330

RAWUL_RING2; RING finger- and WD40-associated ubiquitin-like (RAWUL) domain found in really interesting new gene 2 protein (RING2)
NM_001360847.1 → NP_001347776.1 E3 ubiquitin-protein ligase RING2 isoform b

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform (b).

Source sequence(s)

AC115060, AC165946, AK030319

Consensus CDS

CCDS15362.1

UniProtKB/Swiss-Prot

Q8C1X8

UniProtKB/TrEMBL

A0A6P5QDT0, A0A8C6GSC0

Related

ENSMUSP00000140896.2, ENSMUST00000187048.7

Conserved Domains (3) summary

COG5222
Location:11 → 103

COG5222; Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only]

cd16531
Location:51 → 91

RING-HC_RING1_like; RING finger, HC subclass, found in really interesting new gene proteins RING1, RING2 and similar proteins

cd17167
Location:225 → 330

RAWUL_RING2; RING finger- and WD40-associated ubiquitin-like (RAWUL) domain found in really interesting new gene 2 protein (RING2)
NM_011277.3 → NP_035407.1 E3 ubiquitin-protein ligase RING2 isoform b

See identical proteins and their annotated locations for NP_035407.1

Status: VALIDATED

Description

Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform (b).

Source sequence(s)

AC115060, AC165946, AK012358

Consensus CDS

CCDS15362.1

UniProtKB/Swiss-Prot

Q8C1X8, Q9CQJ4

UniProtKB/TrEMBL

A0A6P5QDT0, A0A8C6GSC0

Related

ENSMUSP00000075476.5, ENSMUST00000076110.11

Conserved Domains (3) summary

COG5222
Location:11 → 103

COG5222; Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only]

cd16531
Location:51 → 91

RING-HC_RING1_like; RING finger, HC subclass, found in really interesting new gene proteins RING1, RING2 and similar proteins

cd17167
Location:225 → 330

RAWUL_RING2; RING finger- and WD40-associated ubiquitin-like (RAWUL) domain found in really interesting new gene 2 protein (RING2)

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

151345149..151376747 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006529269.4 → XP_006529332.1 E3 ubiquitin-protein ligase RING2 isoform X1

Conserved Domains (2) summary

cd16740
Location:56 → 102

RING-HC_RING2; RING finger, HC subclass, found in really interesting new gene 2 protein (RING2) and similar proteins

cd17167
Location:234 → 339

RAWUL_RING2; RING finger- and WD40-associated ubiquitin-like (RAWUL) domain found in really interesting new gene 2 protein (RING2)
XM_036162866.1 → XP_036018759.1 E3 ubiquitin-protein ligase RING2 isoform X2

UniProtKB/Swiss-Prot

Q8C1X8

UniProtKB/TrEMBL

A0A6P5QDT0, A0A8C6GSC0

Conserved Domains (3) summary

COG5222
Location:11 → 103

COG5222; Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only]

cd16531
Location:51 → 91

RING-HC_RING1_like; RING finger, HC subclass, found in really interesting new gene proteins RING1, RING2 and similar proteins

cd17167
Location:225 → 330

RAWUL_RING2; RING finger- and WD40-associated ubiquitin-like (RAWUL) domain found in really interesting new gene 2 protein (RING2)
XM_036162867.1 → XP_036018760.1 E3 ubiquitin-protein ligase RING2 isoform X2

UniProtKB/Swiss-Prot

Q8C1X8

UniProtKB/TrEMBL

A0A6P5QDT0, A0A8C6GSC0

Conserved Domains (3) summary

COG5222
Location:11 → 103

COG5222; Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only]

cd16531
Location:51 → 91

RING-HC_RING1_like; RING finger, HC subclass, found in really interesting new gene proteins RING1, RING2 and similar proteins

cd17167
Location:225 → 330

RAWUL_RING2; RING finger- and WD40-associated ubiquitin-like (RAWUL) domain found in really interesting new gene 2 protein (RING2)