Sqstm1 sequestosome 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Sqstm1provided by MGI
Official Full Name: sequestosome 1provided by MGI
Primary source: MGI:MGI:107931
See related: Ensembl:ENSMUSG00000015837 AllianceGenome:MGI:107931
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Osi; p62; A170; STAP; OSF-6; STONE14
Summary: Enables several functions, including K63-linked polyubiquitin modification-dependent protein binding activity; ionotropic glutamate receptor binding activity; and molecular condensate scaffold activity. Involved in several processes, including aggrephagy; brown fat cell proliferation; and positive regulation of long-term synaptic potentiation. Acts upstream of or within energy homeostasis; negative regulation of transcription by RNA polymerase II; and temperature homeostasis. Located in several cellular components, including aggresome; sperm midpiece; and vacuole. Is active in intracellular membraneless organelle. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; limb; and sensory organ. Used to study Paget's disease of bone. Human ortholog(s) of this gene implicated in Paget's disease of bone; Paget's disease of bone 3; distal myopathy with rimmed vacuoles; frontotemporal dementia and/or amyotrophic lateral sclerosis 3; and neurodegeneration with ataxia, dystonia, and gaze palsy, childhood-onset. Orthologous to human SQSTM1 (sequestosome 1). [provided by Alliance of Genome Resources, Apr 2025]
Expression: Ubiquitous expression in adrenal adult (RPKM 275.7), placenta adult (RPKM 170.1) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 B1.3; 11 30.36 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (50090979..50105303, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (50200152..50210820, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Hypothalamic P62 (SQSTM1) regulates energy balance by modulating leptin signaling.
Title: Hypothalamic P62 (SQSTM1) regulates energy balance by modulating leptin signaling.
P62-autophagic pathway degrades SLC7A11 to regulate ferroptosis in doxorubicin-induced cardiotoxicity.
Title: P62-autophagic pathway degrades SLC7A11 to regulate ferroptosis in doxorubicin-induced cardiotoxicity.
P62 promotes FSH-induced antral follicle formation by directing degradation of ubiquitinated WT1.
Title: P62 promotes FSH-induced antral follicle formation by directing degradation of ubiquitinated WT1.
Sequestosome 1 (p62) mitigates hypoxia-induced cardiac dysfunction by stabilizing hypoxia-inducible factor 1alpha and nuclear factor erythroid 2-related factor 2.
Title: Sequestosome 1 (p62) mitigates hypoxia-induced cardiac dysfunction by stabilizing hypoxia-inducible factor 1α and nuclear factor erythroid 2-related factor 2.
Endothelium-specific deletion of p62 causes organ fibrosis and cardiac dysfunction.
Title: Endothelium-specific deletion of p62 causes organ fibrosis and cardiac dysfunction.
TRIM11 attenuates Treg cell differentiation by p62-selective autophagic degradation of AIM2.
Title: TRIM11 attenuates Treg cell differentiation by p62-selective autophagic degradation of AIM2.
RNF13 protects against pathological cardiac hypertrophy through p62-NRF2 pathway.
Title: RNF13 protects against pathological cardiac hypertrophy through p62-NRF2 pathway.
Muscle p62 stimulates the expression of antioxidant proteins alleviating cancer cachexia.
Title: Muscle p62 stimulates the expression of antioxidant proteins alleviating cancer cachexia.
P62/SQSTM1 binds with claudin-2 to target for selective autophagy in stressed intestinal epithelium.
Title: P62/SQSTM1 binds with claudin-2 to target for selective autophagy in stressed intestinal epithelium.
Caveolin-1 depletion attenuates hepatic fibrosis via promoting SQSTM1-mediated PFKL degradation in HSCs.
Title: Caveolin-1 depletion attenuates hepatic fibrosis via promoting SQSTM1-mediated PFKL degradation in HSCs.

Submit: New GeneRIF Correction See all GeneRIFs (190)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (13) 1 citation
Targeted (13) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI851514 (e-PCR)
- UniSTS:185878

NoName (e-PCR)
- UniSTS:236567

Sqstm1 (e-PCR), detects polymorphism
- UniSTS:536424

AI845619 (e-PCR)
- UniSTS:165749

AW491098 (e-PCR)
- UniSTS:180185

AW475975 (e-PCR)
- UniSTS:180187

Sqstm1 (e-PCR), detects polymorphism
- UniSTS:144097

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables K63-linked polyubiquitin modification-dependent protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables K63-linked polyubiquitin modification-dependent protein binding	IDA Inferred from Direct Assay more info	PubMed
enables K63-linked polyubiquitin modification-dependent protein binding	IEA Inferred from Electronic Annotation more info
enables K63-linked polyubiquitin modification-dependent protein binding	ISO Inferred from Sequence Orthology more info
enables SH2 domain binding	IEA Inferred from Electronic Annotation more info
enables SH2 domain binding	ISO Inferred from Sequence Orthology more info
enables SH2 domain binding	ISS Inferred from Sequence or Structural Similarity more info
enables enzyme binding	IEA Inferred from Electronic Annotation more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables ionotropic glutamate receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables molecular condensate scaffold activity	IDA Inferred from Direct Assay more info	PubMed
enables molecular condensate scaffold activity	IEA Inferred from Electronic Annotation more info
enables molecular condensate scaffold activity	ISO Inferred from Sequence Orthology more info
enables molecular sequestering activity	IEA Inferred from Electronic Annotation more info
enables molecular sequestering activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase C binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase C binding	IEA Inferred from Electronic Annotation more info
enables protein kinase C binding	ISO Inferred from Sequence Orthology more info
enables protein kinase C binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein kinase binding	IEA Inferred from Electronic Annotation more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables protein sequestering activity	IEA Inferred from Electronic Annotation more info
enables protein sequestering activity	ISO Inferred from Sequence Orthology more info
enables protein sequestering activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein-containing complex binding	IEA Inferred from Electronic Annotation more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables protein-macromolecule adaptor activity	IDA Inferred from Direct Assay more info	PubMed
enables protein-macromolecule adaptor activity	IEA Inferred from Electronic Annotation more info
enables protein-macromolecule adaptor activity	ISO Inferred from Sequence Orthology more info
enables signaling adaptor activity	IEA Inferred from Electronic Annotation more info
enables signaling adaptor activity	ISO Inferred from Sequence Orthology more info
enables signaling receptor activity	IEA Inferred from Electronic Annotation more info
enables signaling receptor activity	ISO Inferred from Sequence Orthology more info
enables transcription coregulator activity	TAS Traceable Author Statement more info	PubMed
enables ubiquitin binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin binding	ISS Inferred from Sequence or Structural Similarity more info
enables ubiquitin protein ligase binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin-modified protein reader activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-modified protein reader activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-modified protein reader activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-modified protein reader activity	ISS Inferred from Sequence or Structural Similarity more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in aggrephagy	IBA Inferred from Biological aspect of Ancestor more info
involved_in aggrephagy	IDA Inferred from Direct Assay more info	PubMed
involved_in aggrephagy	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within aggrephagy	ISO Inferred from Sequence Orthology more info
involved_in aggrephagy	ISO Inferred from Sequence Orthology more info
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in autophagy	IDA Inferred from Direct Assay more info	PubMed
involved_in autophagy	IEA Inferred from Electronic Annotation more info
involved_in autophagy	ISO Inferred from Sequence Orthology more info
involved_in autophagy	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within brown fat cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in brown fat cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cellular response to stress	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cellular response to stress	ISO Inferred from Sequence Orthology more info
involved_in cellular response to stress	ISO Inferred from Sequence Orthology more info
involved_in endosome organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in endosome organization	IEA Inferred from Electronic Annotation more info
involved_in endosome organization	ISO Inferred from Sequence Orthology more info
involved_in endosome organization	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within energy homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in energy homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in immune system process	IEA Inferred from Electronic Annotation more info
involved_in macroautophagy	IEA Inferred from Electronic Annotation more info
involved_in macroautophagy	ISO Inferred from Sequence Orthology more info
involved_in macroautophagy	ISS Inferred from Sequence or Structural Similarity more info
involved_in membraneless organelle assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in membraneless organelle assembly	IEA Inferred from Electronic Annotation more info
involved_in membraneless organelle assembly	ISO Inferred from Sequence Orthology more info
involved_in mitophagy	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitophagy	IEA Inferred from Electronic Annotation more info
involved_in mitophagy	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of ferroptosis	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of ferroptosis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of ferroptosis	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein ubiquitination	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of toll-like receptor 4 signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of toll-like receptor 4 signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pexophagy	IEA Inferred from Electronic Annotation more info
involved_in pexophagy	ISO Inferred from Sequence Orthology more info
involved_in pexophagy	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of autophagy	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of autophagy	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of long-term synaptic potentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein localization to plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein K63-linked ubiquitination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein K63-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein import into nucleus	IDA Inferred from Direct Assay more info	PubMed
involved_in protein import into nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
involved_in protein localization to perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
involved_in protein localization to perinuclear region of cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein targeting to vacuole involved in autophagy	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein targeting to vacuole involved in autophagy	ISO Inferred from Sequence Orthology more info
involved_in protein targeting to vacuole involved in autophagy	ISO Inferred from Sequence Orthology more info
involved_in protein targeting to vacuole involved in autophagy	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of canonical NF-kappaB signal transduction	IEA Inferred from Electronic Annotation more info
involved_in regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in regulation of canonical NF-kappaB signal transduction	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of protein complex stability	IEA Inferred from Electronic Annotation more info
involved_in regulation of protein complex stability	ISO Inferred from Sequence Orthology more info
involved_in response to ischemia	IEA Inferred from Electronic Annotation more info
involved_in response to mitochondrial depolarisation	IEA Inferred from Electronic Annotation more info
involved_in response to mitochondrial depolarisation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within temperature homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in temperature homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within toll-like receptor 4 signaling pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within toll-like receptor 4 signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Lewy body	IEA Inferred from Electronic Annotation more info
located_in Lewy body	ISO Inferred from Sequence Orthology more info
located_in P-body	IEA Inferred from Electronic Annotation more info
located_in P-body	ISO Inferred from Sequence Orthology more info
located_in P-body	ISS Inferred from Sequence or Structural Similarity more info
located_in PML body	IEA Inferred from Electronic Annotation more info
located_in PML body	ISO Inferred from Sequence Orthology more info
is_active_in aggresome	IBA Inferred from Biological aspect of Ancestor more info
located_in aggresome	IDA Inferred from Direct Assay more info	PubMed
is_active_in amphisome	IBA Inferred from Biological aspect of Ancestor more info
located_in amphisome	IEA Inferred from Electronic Annotation more info
located_in amphisome	ISO Inferred from Sequence Orthology more info
located_in autolysosome	IDA Inferred from Direct Assay more info	PubMed
located_in autolysosome	IEA Inferred from Electronic Annotation more info
located_in autolysosome	ISO Inferred from Sequence Orthology more info
located_in autophagosome	IDA Inferred from Direct Assay more info	PubMed
located_in autophagosome	IEA Inferred from Electronic Annotation more info
is_active_in autophagosome	ISO Inferred from Sequence Orthology more info
located_in autophagosome	ISO Inferred from Sequence Orthology more info	PubMed
located_in autophagosome	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endosome	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in inclusion body	IEA Inferred from Electronic Annotation more info
located_in inclusion body	ISO Inferred from Sequence Orthology more info
is_active_in intracellular membraneless organelle	IDA Inferred from Direct Assay more info	PubMed
is_active_in intracellular membraneless organelle	ISS Inferred from Sequence or Structural Similarity more info
located_in late endosome	IEA Inferred from Electronic Annotation more info
located_in lysosome	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in phagophore assembly site	IDA Inferred from Direct Assay more info	PubMed
located_in phagophore assembly site	IEA Inferred from Electronic Annotation more info
located_in sarcomere	IEA Inferred from Electronic Annotation more info
located_in sperm midpiece	IDA Inferred from Direct Assay more info	PubMed
is_active_in synapse	IEA Inferred from Electronic Annotation more info
is_active_in synapse	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: sequestosome-1

Names: oxidative stress induced; ubiquitin-binding protein p62

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001290769.1 → NP_001277698.1 sequestosome-1 isoform 2

See identical proteins and their annotated locations for NP_001277698.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region but maintains reading frame, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.

Source sequence(s)

AK028898, BY267273

Consensus CDS

CCDS70176.1

UniProtKB/Swiss-Prot

Q64337

Related

ENSMUSP00000015981.6, ENSMUST00000015981.12

Conserved Domains (3) summary

cd06402
Location:5 → 102

PB1_p62; The PB1 domain is an essential part of p62 scaffold protein (alias sequestosome 1,SQSTM) involved in cell signaling, receptor internalization, and protein turnover. The PB1 domain is a modular domain mediating specific protein-protein interaction which ...

cd02340
Location:126 → 166

ZZ_NBR1_like; Zinc finger, ZZ type. Zinc finger present in Drosophila ref(2)P, NBR1, Human sequestosome 1 and related proteins. The ZZ motif coordinates two zinc ions and most likely participates in ligand binding or molecular scaffolding. Drosophila ref(2)P appears ...

cl21463
Location:353 → 403

UBA_like_SF; UBA domain-like superfamily
NM_011018.3 → NP_035148.1 sequestosome-1 isoform 1

See identical proteins and their annotated locations for NP_035148.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AK028898, BY267273, CX240486

Consensus CDS

CCDS24629.1

UniProtKB/Swiss-Prot

Q64337, Q99JM8

Related

ENSMUSP00000099835.5, ENSMUST00000102774.11

Conserved Domains (3) summary

cd06402
Location:5 → 102

PB1_p62; The PB1 domain is an essential part of p62 scaffold protein (alias sequestosome 1,SQSTM) involved in cell signaling, receptor internalization, and protein turnover. The PB1 domain is a modular domain mediating specific protein-protein interaction which ...

cd02340
Location:126 → 166

ZZ_NBR1_like; Zinc finger, ZZ type. Zinc finger present in Drosophila ref(2)P, NBR1, Human sequestosome 1 and related proteins. The ZZ motif coordinates two zinc ions and most likely participates in ligand binding or molecular scaffolding. Drosophila ref(2)P appears ...

pfam16577
Location:381 → 442

UBA_5; UBA domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

50090979..50105303 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036156414.1 → XP_036012307.1 sequestosome-1 isoform X1

Conserved Domains (3) summary

cd06402
Location:5 → 102

PB1_p62; The PB1 domain is an essential part of p62 scaffold protein (alias sequestosome 1,SQSTM) involved in cell signaling, receptor internalization, and protein turnover. The PB1 domain is a modular domain mediating specific protein-protein interaction which ...

cd02340
Location:126 → 166

ZZ_NBR1_like; Zinc finger, ZZ type. Zinc finger present in Drosophila ref(2)P, NBR1, Human sequestosome 1 and related proteins. The ZZ motif coordinates two zinc ions and most likely participates in ligand binding or molecular scaffolding. Drosophila ref(2)P appears ...

pfam16577
Location:354 → 415

UBA_5; UBA domain