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Nos2 nitric oxide synthase 2, inducible [ Mus musculus (house mouse) ]

Gene ID: 18126, updated on 12-Sep-2021

Summary

Official Symbol
Nos2provided by MGI
Official Full Name
nitric oxide synthase 2, inducibleprovided by MGI
Primary source
MGI:MGI:97361
See related
Ensembl:ENSMUSG00000020826
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
N; No; iNOS; Nos-2; Nos2a; i-NOS; NOS-II; MAC-NOS
Summary
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Expression
Biased expression in large intestine adult (RPKM 30.6), ovary adult (RPKM 13.0) and 6 other tissues See more
Orthologs
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Genomic context

See Nos2 in Genome Data Viewer
Location:
11 B5; 11 46.74 cM
Exon count:
29
Annotation release Status Assembly Chr Location
109 current GRCm39 (GCF_000001635.27) 11 NC_000077.7 (78811613..78851052)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 11 NC_000077.6 (78920787..78960226)
Build 37.2 previous assembly MGSCv37 (GCF_000001635.18) 11 NC_000077.5 (78734361..78773626)

Chromosome 11 - NC_000077.7Genomic Context describing neighboring genes Neighboring gene insulin-like growth factor binding protein 4 pseudogene Neighboring gene predicted gene, 19741 Neighboring gene Nos2 promoter region Neighboring gene lectin, galactose binding, soluble 9 Neighboring gene predicted gene 11201 Neighboring gene kinase suppressor of ras 1

Genomic regions, transcripts, and products

Expression

  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Variation

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (3)  1 citation
  • Endonuclease-mediated (1) 

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables FMN binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
enables FMN binding IDA
Inferred from Direct Assay
more info
PubMed 
enables Hsp90 protein binding ISO
Inferred from Sequence Orthology
more info
 
enables NADP binding IEA
Inferred from Electronic Annotation
more info
 
enables actin binding ISO
Inferred from Sequence Orthology
more info
 
enables arginine binding IDA
Inferred from Direct Assay
more info
PubMed 
enables beta-catenin binding ISO
Inferred from Sequence Orthology
more info
 
enables cAMP-dependent protein kinase regulator activity ISO
Inferred from Sequence Orthology
more info
 
enables cadherin binding ISO
Inferred from Sequence Orthology
more info
 
enables calmodulin binding TAS
Traceable Author Statement
more info
PubMed 
enables flavin adenine dinucleotide binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
enables flavin adenine dinucleotide binding IDA
Inferred from Direct Assay
more info
PubMed 
enables heme binding IDA
Inferred from Direct Assay
more info
PubMed 
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
 
enables nitric-oxide synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
enables nitric-oxide synthase activity IDA
Inferred from Direct Assay
more info
PubMed 
enables nitric-oxide synthase activity ISO
Inferred from Sequence Orthology
more info
 
enables nitric-oxide synthase binding ISO
Inferred from Sequence Orthology
more info
 
enables oxidoreductase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
PubMed 
enables protein kinase binding ISO
Inferred from Sequence Orthology
more info
 
enables tetrahydrobiopterin binding IDA
Inferred from Direct Assay
more info
PubMed 
Process Evidence Code Pubs
involved_in G protein-coupled receptor signaling pathway coupled to cGMP nucleotide second messenger ISO
Inferred from Sequence Orthology
more info
 
involved_in arginine catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in arginine catabolic process IDA
Inferred from Direct Assay
more info
PubMed 
involved_in arginine catabolic process ISO
Inferred from Sequence Orthology
more info
 
involved_in blood vessel remodeling ISO
Inferred from Sequence Orthology
more info
 
involved_in cGMP-mediated signaling ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within cellular response to cytokine stimulus ISO
Inferred from Sequence Orthology
more info
PubMed 
acts_upstream_of_or_within cellular response to interferon-gamma IDA
Inferred from Direct Assay
more info
PubMed 
acts_upstream_of_or_within cellular response to lipopolysaccharide IDA
Inferred from Direct Assay
more info
PubMed 
acts_upstream_of_or_within cellular response to organic cyclic compound ISO
Inferred from Sequence Orthology
more info
PubMed 
acts_upstream_of_or_within cellular response to xenobiotic stimulus IDA
Inferred from Direct Assay
more info
PubMed 
acts_upstream_of_or_within circadian rhythm IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in defense response to bacterium IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
acts_upstream_of_or_within defense response to bacterium IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in defense response to bacterium ISO
Inferred from Sequence Orthology
more info
 
involved_in inflammatory response IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in inflammatory response TAS
Traceable Author Statement
more info
PubMed 
involved_in intracellular signal transduction ISO
Inferred from Sequence Orthology
more info
 
involved_in negative regulation of blood pressure IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in negative regulation of gene expression IGI
Inferred from Genetic Interaction
more info
PubMed 
involved_in negative regulation of gene expression ISO
Inferred from Sequence Orthology
more info
 
involved_in negative regulation of protein catabolic process IDA
Inferred from Direct Assay
more info
PubMed 
involved_in nitric oxide biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in nitric oxide biosynthetic process IDA
Inferred from Direct Assay
more info
PubMed 
involved_in nitric oxide biosynthetic process ISO
Inferred from Sequence Orthology
more info
 
involved_in nitric oxide mediated signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
NOT acts_upstream_of_or_within ovulation from ovarian follicle IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in peptidyl-cysteine S-nitrosylation IDA
Inferred from Direct Assay
more info
PubMed 
involved_in positive regulation of apoptotic process ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of guanylate cyclase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in positive regulation of interleukin-6 production ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of interleukin-8 production ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of killing of cells of other organism ISO
Inferred from Sequence Orthology
more info
 
involved_in prostaglandin secretion ISO
Inferred from Sequence Orthology
more info
 
involved_in regulation of blood pressure ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within regulation of cell population proliferation IGI
Inferred from Genetic Interaction
more info
PubMed 
involved_in regulation of cytokine production involved in inflammatory response ISO
Inferred from Sequence Orthology
more info
 
involved_in regulation of heart contraction ISO
Inferred from Sequence Orthology
more info
 
involved_in regulation of insulin secretion ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within response to bacterium IEP
Inferred from Expression Pattern
more info
PubMed 
involved_in response to hormone IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
acts_upstream_of_or_within response to hypoxia IDA
Inferred from Direct Assay
more info
PubMed 
involved_in response to lipopolysaccharide IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in response to lipopolysaccharide IDA
Inferred from Direct Assay
more info
PubMed 
involved_in response to tumor necrosis factor ISO
Inferred from Sequence Orthology
more info
 
involved_in signal transduction ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within superoxide metabolic process IMP
Inferred from Mutant Phenotype
more info
PubMed 
Component Evidence Code Pubs
located_in cortical cytoskeleton IDA
Inferred from Direct Assay
more info
PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
located_in cytosol ISO
Inferred from Sequence Orthology
more info
 
located_in cytosol TAS
Traceable Author Statement
more info
PubMed 
located_in extracellular space ISO
Inferred from Sequence Orthology
more info
 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
located_in nucleus ISO
Inferred from Sequence Orthology
more info
 
located_in perinuclear region of cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
located_in perinuclear region of cytoplasm ISO
Inferred from Sequence Orthology
more info
 
located_in peroxisome ISO
Inferred from Sequence Orthology
more info
 
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
is_active_in vesicle membrane IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
located_in vesicle membrane ISO
Inferred from Sequence Orthology
more info
 

General protein information

Preferred Names
nitric oxide synthase, inducible
Names
NOS type II
inducible NO synthase
inducible NOS
inducible nitric oxide synthase
macrophage NOS
nitric oxide synthase 2, inducible, macrophage
peptidyl-cysteine S-nitrosylase NOS2
NP_001300850.1
NP_001300851.1
NP_035057.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001313921.1NP_001300850.1  nitric oxide synthase, inducible isoform b

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) contains an alternate exon in place of the first two exons compared to variant 1. This difference results in translation initiation at a downstream start codon and an isoform (b) that is shorter at the N-terminus compared to isoform a. Variants 2 and 3 both encode the same isoform (b).
    Source sequence(s)
    AL592185, CJ057491, M87039
    UniProtKB/Swiss-Prot
    P29477
    UniProtKB/TrEMBL
    A0A1L1STZ4
    Conserved Domains (4) summary
    COG0369
    Location:4211008
    CysJ; Sulfite reductase, alpha subunit (flavoprotein) [Inorganic ion transport and metabolism]
    cd00795
    Location:1377
    NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...
    pfam00258
    Location:422553
    Flavodoxin_1; Flavodoxin
    cl06868
    Location:6171013
    FNR_like; Ferredoxin reductase (FNR), an FAD and NAD(P) binding protein, was intially identified as a chloroplast reductase activity, catalyzing the electron transfer from reduced iron-sulfur protein ferredoxin to NADP+ as the final step in the electron transport ...
  2. NM_001313922.1NP_001300851.1  nitric oxide synthase, inducible isoform b

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) contains an alternate exon in place of the first two exons compared to variant 1. This difference results in translation initiation at a downstream start codon and an isoform (b) that is shorter at the N-terminus compared to isoform a. Variants 2 and 3 both encode the same isoform (b).
    Source sequence(s)
    AL592185, CN693247, M87039
    UniProtKB/Swiss-Prot
    P29477
    UniProtKB/TrEMBL
    A0A1L1STZ4
    Related
    ENSMUSP00000150558.3, ENSMUST00000214397.3
    Conserved Domains (4) summary
    COG0369
    Location:4211008
    CysJ; Sulfite reductase, alpha subunit (flavoprotein) [Inorganic ion transport and metabolism]
    cd00795
    Location:1377
    NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...
    pfam00258
    Location:422553
    Flavodoxin_1; Flavodoxin
    cl06868
    Location:6171013
    FNR_like; Ferredoxin reductase (FNR), an FAD and NAD(P) binding protein, was intially identified as a chloroplast reductase activity, catalyzing the electron transfer from reduced iron-sulfur protein ferredoxin to NADP+ as the final step in the electron transport ...
  3. NM_010927.4NP_035057.1  nitric oxide synthase, inducible isoform a

    See identical proteins and their annotated locations for NP_035057.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a).
    Source sequence(s)
    AL592185, M87039
    Consensus CDS
    CCDS25115.1
    UniProtKB/Swiss-Prot
    P29477
    Related
    ENSMUSP00000018610.7, ENSMUST00000018610.7
    Conserved Domains (4) summary
    COG0369
    Location:5341121
    CysJ; Sulfite reductase, alpha subunit (flavoprotein) [Inorganic ion transport and metabolism]
    cd00795
    Location:78490
    NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...
    pfam00258
    Location:535666
    Flavodoxin_1; Flavodoxin
    cl06868
    Location:7301126
    FNR_like; Ferredoxin reductase (FNR), an FAD and NAD(P) binding protein, was intially identified as a chloroplast reductase activity, catalyzing the electron transfer from reduced iron-sulfur protein ferredoxin to NADP+ as the final step in the electron transport ...

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000077.7 Reference GRCm39 C57BL/6J

    Range
    78811613..78851052
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
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