Ncl nucleolin [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nclprovided by MGI
Official Full Name: nucleolinprovided by MGI
Primary source: MGI:MGI:97286
See related: Ensembl:ENSMUSG00000026234 AllianceGenome:MGI:97286
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: C23; Nucl; D0Nds28; D1Nds28; B530004O11Rik
Summary: Enables RNA binding activity. Acts upstream of or within cellular response to epidermal growth factor stimulus; cellular response to leukemia inhibitory factor; and positive regulation of transcription by RNA polymerase II. Located in nucleolus and nucleoplasm. Is expressed in several structures, including 1st branchial arch mandibular component; blastocyst; central nervous system; early embryo; and tooth. Orthologous to human NCL (nucleolin). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in CNS E11.5 (RPKM 52.4), liver E14 (RPKM 41.1) and 11 other tissues See more
Orthologs: human all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 1 C5; 1 43.94 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
109	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (86272441..86287177, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (86344719..86359455, complement)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help

See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The glycine arginine-rich domain of the RNA-binding protein nucleolin regulates its subcellular localization.
Title: The glycine arginine-rich domain of the RNA-binding protein nucleolin regulates its subcellular localization.
LncRNA H19 is involved in myocardial ischemic preconditioning via increasing the stability of nucleolin protein.
Title: LncRNA H19 is involved in myocardial ischemic preconditioning via increasing the stability of nucleolin protein.
Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.
Title: Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.
Growth Factor Midkine Aggravates Pulmonary Arterial Hypertension via Surface Nucleolin.
Title: Growth Factor Midkine Aggravates Pulmonary Arterial Hypertension via Surface Nucleolin.
Inhibiting nucleolin reduces inflammation induced by mitochondrial DNA in cardiomyocytes exposed to hypoxia and reoxygenation.
Title: Inhibiting nucleolin reduces inflammation induced by mitochondrial DNA in cardiomyocytes exposed to hypoxia and reoxygenation.
TLR4 and nucleolin influence cell injury, apoptosis and inflammatory factor expression in respiratory syncytial virus-infected N2a neuronal cells.
Title: TLR4 and nucleolin influence cell injury, apoptosis and inflammatory factor expression in respiratory syncytial virus-infected N2a neuronal cells.
Phosphorylated nucleolin interacted with Ago2 in the cytoplasm to protect the cardiomyocytes from oxidative stress by up-expression of miR-21.
Title: Phosphorylation of nucleolin is indispensable to upregulate miR-21 and inhibit apoptosis in cardiomyocytes.
Study in RAW264.7 and HeLa cells revealed that Bothrops asper myotoxin-II (Mt-II) and nucleolin (NCL) were shown to colocalise, at 4 degrees C, on cell membrane, while at 37 degrees C, 20 minutes after the intoxication, they colocalise in intracellular spots, and observed that, by lowering NCL expression by RNA interference in Hela cells, the sensitivity of these cells to Mt-II cytotoxicity is considerably decreased.
Title: Cell surface nucleolin interacts with and internalizes Bothrops asper Lys49 phospholipase A<sub>2</sub> and mediates its toxic activity.
These results have demonstrated that nucleolin is involved in the regulation of Doxorubicin-induced cardiac injury and dysfunction via the regulation of miRNA-21.
Title: Nucleolin protects against doxorubicin-induced cardiotoxicity via upregulating microRNA-21.
The results demonstrated prolific RSV infection of N2a cells, which triggered a decrease of NeuN protein expression, coinciding with an increase of nuclear lesions, F protein expression, RSV viral titers, and late apoptotic levels of N2a cells. RSV infection induced co-localization of RSV F protein with TLR4 and nucleolin.
Title: Respiratory syncytial virus prolifically infects N2a neuronal cells, leading to TLR4 and nucleolin protein modulations and RSV F protein co-localization with TLR4 and nucleolin.

Submit: New GeneRIF Correction See all GeneRIFs (34)

Variation

Go to the top of the page Help

Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2) 1 citation

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

Ncl (e-PCR), detects polymorphism
- UniSTS:532321

Ncl (e-PCR), detects polymorphism
- UniSTS:143994

D1S3697 (e-PCR)
- UniSTS:474275

D1Mit516 (e-PCR), detects polymorphism
- UniSTS:142252

Ncl (e-PCR), detects polymorphism
- UniSTS:479178

Homology

Homologs of the Ncl gene: The Ncl gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, and rat.
Orthologs from Annotation Pipeline: 463 organisms have orthologs with human gene Ncl
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA topoisomerase binding	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables ErbB-4 class receptor binding	ISO Inferred from Sequence Orthology more info
enables PH domain binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA binding	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA binding	ISO Inferred from Sequence Orthology more info
enables calcium ion binding	ISO Inferred from Sequence Orthology more info
enables histone binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables insulin receptor substrate binding	IPI Inferred from Physical Interaction more info	PubMed
enables laminin binding	ISO Inferred from Sequence Orthology more info
enables mRNA 5'-UTR binding	ISO Inferred from Sequence Orthology more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables rRNA primary transcript binding	ISO Inferred from Sequence Orthology more info
enables selenocysteine insertion sequence binding	ISO Inferred from Sequence Orthology more info
enables sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables signaling receptor binding	ISO Inferred from Sequence Orthology more info
enables single-stranded DNA binding	ISO Inferred from Sequence Orthology more info
enables telomeric DNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in angiogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to epidermal growth factor stimulus	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cellular response to leukemia inhibitory factor	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to lipopolysaccharide	ISO Inferred from Sequence Orthology more info
involved_in endocytosis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of insulin receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of translation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interleukin-6 production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of mRNA splicing, via spliceosome	IBA Inferred from Biological aspect of Ancestor more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription of nucleolar large rRNA by RNA polymerase I	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of tumor necrosis factor production	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of peptidyl-tyrosine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of rRNA processing	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cell cortex	ISO Inferred from Sequence Orthology more info
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in chromosome	ISO Inferred from Sequence Orthology more info
is_active_in cornified envelope	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic ribonucleoprotein granule	ISO Inferred from Sequence Orthology more info
located_in dense fibrillar component	ISO Inferred from Sequence Orthology more info
located_in fibrillar center	ISO Inferred from Sequence Orthology more info
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of ribonucleoprotein complex	ISO Inferred from Sequence Orthology more info	PubMed
part_of spliceosomal complex	IBA Inferred from Biological aspect of Ancestor more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: nucleolin

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_010880.3 → NP_035010.3 nucleolin

See identical proteins and their annotated locations for NP_035010.3

Status: VALIDATED

Source sequence(s)

AC102609, AK144894, CJ084318

Consensus CDS

CCDS35646.1

UniProtKB/Swiss-Prot

P09405, Q99K50

Related

ENSMUSP00000027438.7, ENSMUST00000027438.8

Conserved Domains (4) summary

cd12403
Location:309 → 383

RRM1_NCL; RNA recognition motif 1 in vertebrate nucleolin

cd12404
Location:392 → 467

RRM2_NCL; RNA recognition motif 2 in vertebrate nucleolin

cd12405
Location:486 → 558

RRM3_NCL; RNA recognition motif 3 in vertebrate nucleolin

cd12406
Location:569 → 646

RRM4_NCL; RNA recognition motif 4 in vertebrate nucleolin

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

86272441..86287177 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006529238.4 → XP_006529301.1 nucleolin isoform X1

Conserved Domains (5) summary

PRK10905
Location:34 → 124

PRK10905; cell division protein DamX; Validated

cd12403
Location:293 → 367

RRM1_NCL; RNA recognition motif 1 in vertebrate nucleolin

cd12404
Location:376 → 451

RRM2_NCL; RNA recognition motif 2 in vertebrate nucleolin

cd12405
Location:470 → 542

RRM3_NCL; RNA recognition motif 3 in vertebrate nucleolin

cd12406
Location:553 → 630

RRM4_NCL; RNA recognition motif 4 in vertebrate nucleolin