Nck1 non-catalytic region of tyrosine kinase adaptor protein 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nck1provided by MGI
Official Full Name: non-catalytic region of tyrosine kinase adaptor protein 1provided by MGI
Primary source: MGI:MGI:109601
See related: Ensembl:ENSMUSG00000032475 AllianceGenome:MGI:109601
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Nck; Nck-1; 6330586M15Rik; D230010O13Rik
Summary: Enables ephrin receptor binding activity; eukaryotic initiation factor eIF2 binding activity; and protein domain specific binding activity. Involved in regulation of cellular macromolecule biosynthetic process and regulation of response to endoplasmic reticulum stress. Acts upstream of or within several processes, including ephrin receptor signaling pathway; plasma membrane bounded cell projection assembly; and positive regulation of cellular component organization. Located in cell-cell junction and vesicle membrane. Is expressed in several structures, including extraembryonic component; hemolymphoid system gland; male reproductive gland or organ; nervous system; and somite. Orthologous to human NCK1 (NCK adaptor protein 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in CNS E11.5 (RPKM 10.7), placenta adult (RPKM 9.3) and 24 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 E3.3; 9 52.69 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (100376047..100428187, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (100494302..100546134, complement)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Distinct Requirements for Adaptor Proteins NCK1 and NCK2 in Mammary Gland Development.
Title: Distinct Requirements for Adaptor Proteins NCK1 and NCK2 in Mammary Gland Development.
NCK1 Modulates Neuronal Actin Dynamics and Promotes Dendritic Spine, Synapse, and Memory Formation.
Title: NCK1 Modulates Neuronal Actin Dynamics and Promotes Dendritic Spine, Synapse, and Memory Formation.
Activation of the PDGFRalpha-Nrf2 pathway mediates impaired adipocyte differentiation in bone marrow mesenchymal stem cells lacking Nck1.
Title: Activation of the PDGFRα-Nrf2 pathway mediates impaired adipocyte differentiation in bone marrow mesenchymal stem cells lacking Nck1.
Nck1, But Not Nck2, Mediates Disturbed Flow-Induced p21-Activated Kinase Activation and Endothelial Permeability.
Title: Nck1, But Not Nck2, Mediates Disturbed Flow-Induced p21-Activated Kinase Activation and Endothelial Permeability.
Selective role of Nck1 in atherogenic inflammation and plaque formation.
Title: Selective role of Nck1 in atherogenic inflammation and plaque formation.
SHIP interacts with adaptor protein Nck and restricts actin turnover in B cells.
Title: SHIP interacts with adaptor protein Nck and restricts actin turnover in B cells.
Nck1 silencing in pancreatic beta cells promotes PERK activation and signaling to protect beta cells against pathological stresses.
Title: Nck1 deficiency improves pancreatic β cell survival to diabetes-relevant stresses by modulating PERK activation and signaling.
In mice with Nck1/2 deletions, endothelial cells fail to develop front-rear polarized vessel sprouts.
Title: Targeting NCK-Mediated Endothelial Cell Front-Rear Polarity Inhibits Neovascularization.
These results reveal that Nck regulates preosteoblastic/osteoblastic migration and bone mass.
Title: Nck influences preosteoblastic/osteoblastic migration and bone mass.
we show that the non-catalytic region of tyrosine kinase adaptor (NCK) proteins 1 and 2 are distributed in the developing spinal cord
Title: NCK is critical for the development of deleted in colorectal cancer (DCC) sensitive spinal circuits.

Submit: New GeneRIF Correction See all GeneRIFs (44)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (4) 1 citation
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ephrin receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables ephrin receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables eukaryotic initiation factor eIF2 binding	IPI Inferred from Physical Interaction more info	PubMed
enables eukaryotic initiation factor eIF2 binding	ISO Inferred from Sequence Orthology more info
enables molecular condensate scaffold activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase inhibitor activity	ISO Inferred from Sequence Orthology more info
enables protein-macromolecule adaptor activity	ISO Inferred from Sequence Orthology more info
enables receptor tyrosine kinase binding	IBA Inferred from Biological aspect of Ancestor more info
enables receptor tyrosine kinase binding	ISO Inferred from Sequence Orthology more info
enables signaling adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables signaling receptor binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in T cell activation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within actin filament organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in antiviral innate immune response	ISO Inferred from Sequence Orthology more info
involved_in cell migration	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ephrin receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within ephrin receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lamellipodium assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of PERK-mediated unfolded protein response	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of PERK-mediated unfolded protein response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of PERK-mediated unfolded protein response	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of insulin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of T cell proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of actin filament polymerization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of actin filament polymerization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cap-dependent translational initiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cap-independent translational initiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of neuron projection development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of peptidyl-serine dephosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of translation in response to endoplasmic reticulum stress	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of translation in response to endoplasmic reticulum stress	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of translation in response to endoplasmic reticulum stress	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of cell migration	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in response to endoplasmic reticulum stress	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to endoplasmic reticulum stress	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to other organism	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within substrate-dependent cell migration, cell extension	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cell-cell junction	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
part_of protein phosphatase type 1 complex	ISO Inferred from Sequence Orthology more info
located_in ribosome	ISO Inferred from Sequence Orthology more info
located_in vesicle membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: SH2/SH3 adapter protein Nck1

Names: NCK adapter protein 1; NCK adaptor protein 1; cytoplasmic protein NCK1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001324530.1 → NP_001311459.1 SH2/SH3 adapter protein Nck1 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses a distinct promoter and therefore has a distinct 5' UTR and 5' coding region, compared to variant 1. This variant encodes isoform 2 which, compared to isoform 1, lacks an N-terminal SH3 domain.

Source sequence(s)

AK053155, AK054051

Consensus CDS

CCDS85715.1

UniProtKB/TrEMBL

Q8BH99

Related

ENSMUSP00000108495.4, ENSMUST00000112874.4

Conserved Domains (3) summary

cd10408
Location:216 → 312

SH2_Nck1; Src homology 2 (SH2) domain found in Nck

cd11901
Location:44 → 98

SH3_Nck1_2; Second Src Homology 3 domain of Nck1 adaptor protein

cd11904
Location:129 → 185

SH3_Nck1_3; Third Src Homology 3 domain of Nck1 adaptor protein
NM_010878.3 → NP_035008.2 SH2/SH3 adapter protein Nck1 isoform 1

See identical proteins and their annotated locations for NP_035008.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AK053155, BY096444, CJ171968

Consensus CDS

CCDS23440.1

UniProtKB/Swiss-Prot

O55032, Q99M51, Q9Z279

Related

ENSMUSP00000112221.2, ENSMUST00000116522.8

Conserved Domains (4) summary

cd10408
Location:280 → 376

SH2_Nck1; Src homology 2 (SH2) domain found in Nck

cd11900
Location:3 → 61

SH3_Nck1_1; First Src Homology 3 domain of Nck1 adaptor protein

cd11901
Location:108 → 162

SH3_Nck1_2; Second Src Homology 3 domain of Nck1 adaptor protein

cd11904
Location:193 → 249

SH3_Nck1_3; Third Src Homology 3 domain of Nck1 adaptor protein

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

100376047..100428187 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006510840.4 → XP_006510903.1 SH2/SH3 adapter protein Nck1 isoform X1

See identical proteins and their annotated locations for XP_006510903.1

UniProtKB/Swiss-Prot

O55032, Q99M51, Q9Z279

Conserved Domains (4) summary

cd10408
Location:280 → 376

SH2_Nck1; Src homology 2 (SH2) domain found in Nck

cd11900
Location:3 → 61

SH3_Nck1_1; First Src Homology 3 domain of Nck1 adaptor protein

cd11901
Location:108 → 162

SH3_Nck1_2; Second Src Homology 3 domain of Nck1 adaptor protein

cd11904
Location:193 → 249

SH3_Nck1_3; Third Src Homology 3 domain of Nck1 adaptor protein