lgg-2 Protein lgg;Protein lgg-2 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: lgg-2
Official Full Name: Protein lgg;Protein lgg-2
Primary source: WormBase:WBGene00002981
Locus tag: CELE_ZK593.6
See related: AllianceGenome:WB:WBGene00002981
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable microtubule binding activity; phosphatidylethanolamine binding activity; and ubiquitin protein ligase binding activity. Involved in cellular response to toxic substance; defense response to other organism; and positive regulation of autophagosome maturation. Acts upstream of or within with a positive effect on plasma membrane repair. Located in autophagosome membrane. Is expressed in several structures, including intestine; muscle cell; pharynx; spermatheca; and vulva. Human ortholog(s) of this gene implicated in glioblastoma. Orthologous to several human genes including MAP1LC3A (microtubule associated protein 1 light chain 3 alpha) and MAP1LC3B (microtubule associated protein 1 light chain 3 beta). [provided by Alliance of Genome Resources, Apr 2025]
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Genomic context

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See lgg-2 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 3

Sequence:: Chromosome: IV; NC_003282.8 (10934196..10935903, complement)

Chromosome IV - NC_003282.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003282.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Autophagy-associated proteins, including the Beclin 1 homolog BEC-1 and the Atg8/LC3-family members LGG-1 and LGG-2, localize around the midbody phagosome and are required for midbody degradation.
Title: C. elegans midbodies are released, phagocytosed and undergo LC3-dependent degradation independent of macroautophagy.
LGG-1 is involved in the exposure of the 'eat-me signal' phosphatidylserine at the surface of the apoptotic cell to allow its recognition by the phagocytic cell, whereas LGG-2 is involved in later steps of phagocytosis to allow efficient cell corpse clearance by mediating the maturation/degradation of the phagosome.
Title: Autophagy mediates phosphatidylserine exposure and phagosome degradation during apoptosis through specific functions of GABARAP/LGG-1 and LC3/LGG-2.
we found that LGG-2/LC3 regulates Nematocida colonization inside intestinal cells. Interestingly, LGG-2/LC3 regulates intracellular colonization only in the HW strain, and not in N2. Altogether these results demonstrate that there is natural genetic variation in an LGG-2-dependent process that regulates microsporidia colonization inside intestinal cells, although not microsporidia clearance
Title: Natural variation in the roles of C. elegans autophagy components during microsporidia infection.
This work analyzed in apoptotic cell and phagocytic cell the localization of the two homologs of LC3/GABARAP proteins, namely, LGG-1 and LGG-2.
Title: Correlative Light and Electron Microscopy to Analyze LC3 Proteins in Caenorhabditis elegans Embryo.
Our results indicate that an ARF-like GTPase gene product, ARL-8, mitigates endocytic Ab neurodegeneration in a VPS-41-dependent manner, rather than through RAB-7 and AP3 as with alpha-synuclein..we demonstrate that the LC3 orthologue, LGG-2, plays a critical role in Ab toxicity with ARL-8.
Title: Distinct functional roles of Vps41-mediated neuroprotection in Alzheimer's and Parkinson's disease models of neurodegeneration.
Data show the N-terminal of LGG-1 and LGG-2 adopts the closed and open form, respectively. They have distinct binding pockets that prefer different residues, influencing binding specificity and differential interaction with autophagy proteins.
Title: Structural Basis of the Differential Function of the Two C. elegans Atg8 Homologs, LGG-1 and LGG-2, in Autophagy.
the major contribution of LGG-2 in sperm-inherited organelle clearance resides in its capacity to mediate the retrograde transport of autophagosomes rather than their fusion with acidic compartments.
Title: Sperm-inherited organelle clearance in C. elegans relies on LC3-dependent autophagosome targeting to the pericentrosomal area.
LGG-2 interacts with VPS-39 to tether autophagosomes and lysosomes.
Title: The C. elegans LC3 acts downstream of GABARAP to degrade autophagosomes by interacting with the HOPS subunit VPS39.
both LGG-2 and LGG-1 act synergistically and are involved in dauer formation and longevity of the worm.
Title: The autophagosomal protein LGG-2 acts synergistically with LGG-1 in dauer formation and longevity in C. elegans.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

ZK593.6 (e-PCR)
- UniSTS:318750

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylethanolamine binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in autophagosome assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in autophagosome maturation	IBA Inferred from Biological aspect of Ancestor more info
involved_in autophagy	IEA Inferred from Electronic Annotation more info
involved_in cellular response to nitrogen starvation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to toxic substance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in defense response to Gram-positive bacterium	IEP Inferred from Expression Pattern more info	PubMed
involved_in macroautophagy	IEA Inferred from Electronic Annotation more info
involved_in mitophagy	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within_positive_effect plasma membrane repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of autophagosome maturation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to stress	IEA Inferred from Electronic Annotation more info
involved_in xenophagy	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in autophagosome	IEA Inferred from Electronic Annotation more info
is_active_in autophagosome membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in autophagosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Protein lgg;Protein lgg-2

NP_001255523.2

Confirmed by transcript evidence

NP_001255524.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003282.8 Reference assembly

Range

10934196..10935903 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001268594.4 → NP_001255523.2 Protein lgg-2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q23536

Conserved Domains (1) summary

cd01611
Location:29 → 142

GABARAP; Ubiquitin domain of GABA-receptor-associated protein
NM_001268595.3 → NP_001255524.1 Protein lgg

See identical proteins and their annotated locations for NP_001255524.1

Status: REVIEWED

UniProtKB/TrEMBL

E2JKY9

Conserved Domains (1) summary

cl00155
Location:1 → 74

UBQ; Ubiquitin-like proteins