pie-1 Pharynx and intestine in excess protein 1 [Caenorhabditis elegans] - Gene

Summary

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Gene symbol: pie-1
Gene description: Pharynx and intestine in excess protein 1
Primary source: WormBase:WBGene00004027
Locus tag: CELE_Y49E10.14
See related: AllianceGenome:WB:WBGene00004027
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable mRNA 3'-UTR binding activity. Involved in several processes, including embryonic pattern specification; formation of primary germ layer; and negative regulation of nitrogen compound metabolic process. Located in several cellular components, including P granule; centrosome; and cytosol. Is expressed in several structures, including P0; germ line; head muscle; nerve ring; and oocyte. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: III

Exon count:: 5

Sequence:: Chromosome: III; NC_003281.10 (12426893..12429195)

Chromosome III - NC_003281.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003281.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PIE-1 SUMOylation promotes germline fates and piRNA-dependent silencing in C. elegans.
Title: PIE-1 SUMOylation promotes germline fates and piRNA-dependent silencing in C. elegans.
Y14 and MAG-1 do not regulate PIE-1 degradation, segregation or synthesis in the early embryo, but do regulate the concentration of maternally-deposited PIE-1::GFP. Taken together, or findings point to an important role for translational control in the regulation of PIE-1 levels in the germline lineage.
Title: PIE-1 Translation in the Germline Lineage Contributes to PIE-1 Asymmetry in the Early Caenorhabditis elegans Embryo.
Results support a heterogeneous reaction/diffusion model for PIE-1 enrichment in which the diffusion coefficient of PIE-1 is reversibly reduced in the posterior, resulting in a stable protein gradient across the zygote at steady state.
Title: Asymmetric enrichment of PIE-1 in the Caenorhabditis elegans zygote mediated by binary counterdiffusion.
These results suggest that PIE-1 uses partially redundant mechanisms to block transcription by targeting both the initiation and elongation phases of the transcription cycle.
Title: Inhibition of transcription by the Caenorhabditis elegans germline protein PIE-1: genetic evidence for distinct mechanisms targeting initiation and elongation.
Degradation of this maternally supplied cell fate determinant is delayed in oma-1 (zu405) mutant embryos.
Title: A gain-of-function mutation in oma-1, a C. elegans gene required for oocyte maturation, results in delayed degradation of maternal proteins and embryonic lethality.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Y49E10.14 (e-PCR)
- UniSTS:319946

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA 3'-UTR binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cell fate determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin remodeling	IDA Inferred from Direct Assay more info	PubMed
involved_in embryonic pattern specification	IGI Inferred from Genetic Interaction more info	PubMed
involved_in embryonic pattern specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in formation of primary germ layer	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in P granule	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in intracellular organelle	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in spindle	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Pharynx and intestine in excess protein 1

NP_001255166.1

Confirmed by transcript evidence

NP_001255167.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003281.10 Reference assembly

Range

12426893..12429195

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001268237.2 → NP_001255166.1 Pharynx and intestine in excess protein 1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001255166.1

Status: REVIEWED

UniProtKB/Swiss-Prot

E9P8A6, Q94131, Q9XTT5

Conserved Domains (2) summary

smart00356
Location:184 → 210

ZnF_C3H1; zinc finger

pfam00642
Location:99 → 125

zf-CCCH; Zinc finger C-x8-C-x5-C-x3-H type (and similar)
NM_001268238.3 → NP_001255167.1 Pharynx and intestine in excess protein 1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001255167.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q94131

Conserved Domains (1) summary

PRK15273
Location:46 → 86

PRK15273; putative fimbrial outer membrane usher protein SteB; Provisional