fbf-2 Fem-3 mRNA-binding factor 2 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: fbf-2
Official Full Name: Fem-3 mRNA-binding factor 2
Primary source: WormBase:WBGene00001402
Locus tag: CELE_F21H12.5
See related: AllianceGenome:WB:WBGene00001402
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables mRNA 3'-UTR binding activity. Involved in negative regulation of translation. Located in P granule. Is expressed in germ line. Used to study obesity. [provided by Alliance of Genome Resources, Jul 2025]
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Genomic context

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See fbf-2 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 8

Sequence:: Chromosome: II; NC_003280.10 (6088863..6091480, complement)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

COP9 signalosome component CSN-5 stabilizes PUF proteins FBF-1 and FBF-2 in Caenorhabditis elegans germline stem and progenitor cells.
Title: COP9 signalosome component CSN-5 stabilizes PUF proteins FBF-1 and FBF-2 in Caenorhabditis elegans germline stem and progenitor cells.
Antagonistic control of Caenorhabditis elegans germline stem cell proliferation and differentiation by PUF proteins FBF-1 and FBF-2.
Title: Antagonistic control of Caenorhabditis elegans germline stem cell proliferation and differentiation by PUF proteins FBF-1 and FBF-2.
LST-1 weakens FBF-2 binding affinity for short and long motifs, which may increase target selectivity.
Title: A crystal structure of a collaborative RNA regulatory complex reveals mechanisms to refine target specificity.
Data indicate that a 10-amino-acid region in germline development defective-3 (GLD-3) is required for fem-3 binding factor (FBF) binding, while a 7-amino-acid loop in FBF between PUF repeats 7 and 8 is necessary for GLD-3 binding.
Title: A protein.protein interaction platform involved in recruitment of GLD-3 to the FBF.fem-3 mRNA complex.
Data show that CPB-1 (cytoplasmic polyadenylation element binding protein homolog-1) alters the affinity of FBF (fem-3 mRNA binding factor) for specific RNA sequences.
Title: Biochemical characterization of the Caenorhabditis elegans FBF.CPB-1 translational regulation complex identifies conserved protein interaction hotspots.
findings show FBF-1 and FBF-2 have different effects on target mRNAs; FBF-2 prevents translation; FBF-2 activity depends on PGL-1; PGL-1 is required to localize FBF-2 to perinuclear P granules and for efficient binding of FBF-2 to its mRNA targets
Title: The P granule component PGL-1 promotes the localization and silencing activity of the PUF protein FBF-2 in germline stem cells.
Data suggest that the FBF-2/GLD-2 protein directed polyadenylation of the endogenous mRNA, compensating for that mRNA's natural deadenylation.
Title: Targeted translational regulation using the PUF protein family scaffold.
The Puf RNA-binding proteins FBF-1 and FBF-2 inhibit the expression of synaptonemal complex proteins in germline stem cells.
Title: The Puf RNA-binding proteins FBF-1 and FBF-2 inhibit the expression of synaptonemal complex proteins in germline stem cells.
fbf-2 regulates the size of the mitotic region in helminth germ cells.
Title: FBF-1 and FBF-2 regulate the size of the mitotic region in the C. elegans germline.
FBF may coordinately repress sperm-specifying mRNAs to direct oogenesis and it represses the fog-1 mRNA to maintain FOG-1 at a low level appropriate for proliferation
Title: Dose-dependent control of proliferation and sperm specification by FOG-1/CPEB.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

F21H12.5 (e-PCR)
- UniSTS:309877

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables RNA binding	EXP Inferred from Experiment more info	PubMed
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA 3'-UTR binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA 3'-UTR binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in negative regulation of translation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in post-transcriptional regulation of gene expression	IBA Inferred from Biological aspect of Ancestor more info
involved_in post-transcriptional regulation of gene expression	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in P granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Fem-3 mRNA-binding factor 2

NP_001367592.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003280.10 Reference assembly

Range

6088863..6091480 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001381445.2 → NP_001367592.1 Fem-3 mRNA-binding factor 2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q09312

Conserved Domains (1) summary

cd07920
Location:182 → 562

Pumilio; Pumilio-family RNA binding domain