Mgrn1 mahogunin, ring finger 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mgrn1provided by MGI
Official Full Name: mahogunin, ring finger 1provided by MGI
Primary source: MGI:MGI:2447670
See related: Ensembl:ENSMUSG00000022517 AllianceGenome:MGI:2447670
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: md; nc; mKIAA0544; 2610042J20Rik
Summary: Enables ubiquitin protein ligase activity. Involved in negative regulation of smoothened signaling pathway. Acts upstream of or within protein polyubiquitination. Predicted to be located in endoplasmic reticulum. Predicted to be active in early endosome; nucleus; and plasma membrane. Is expressed in several structures, including genitourinary system; heart; node; and skin. Orthologous to human MGRN1 (mahogunin ring finger 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in heart adult (RPKM 66.4), ovary adult (RPKM 57.2) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 16 A1; 16 2.48 cM

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	16	NC_000082.7 (4703964..4756160)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	16	NC_000082.6 (4886100..4938296)

Chromosome 16 - NC_000082.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Distribution and Localization of Mahogunin Ring Finger 1 in the Mouse Central Nervous System.
Title: Distribution and Localization of Mahogunin Ring Finger 1 in the Mouse Central Nervous System.
Mahogunin Ring Finger 1 regulates pigmentation by controlling the pH of melanosomes in melanocytes and melanoma cells.
Title: Mahogunin Ring Finger 1 regulates pigmentation by controlling the pH of melanosomes in melanocytes and melanoma cells.
E3 ubiquitin-protein ligase MGRN1 (MGRN1) localizes to mitochondria, and mitochondria in cells from Mgrn1 null mutant mice display fragmentation and depolarization without recruitment of the parkin E3 ubiquitin ligase. The late onset of pathology in the brains of Mgrn1 null mutant mice suggests that a further, age-dependent effect on mitochondrial homeostasis is required to trigger vacuolation.
Title: Chronic and age-dependent effects of the spongiform neurodegeneration-associated MGRN1 E3 ubiquitin ligase on mitochondrial homeostasis.
Calmodulin regulates MGRN1-GP78 interaction mediated ubiquitin proteasomal degradation system.
Title: Calmodulin regulates MGRN1-GP78 interaction mediated ubiquitin proteasomal degradation system.
Mgrn1 can negatively regulate signaling through the Hedgehog pathway by reducing cell-surface and ciliary levels of Smoothened, an oncoprotein that transmits the Hedgehog signal across the membrane.
Title: CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog.
With aging, the neuroprotective e3 ligase MGRN1 relocates from the cytosol to the nucleus of neurons, where it associates with chromatin and potentiates the cellular response to proteotoxic stress. This nuclear shift is due to a proteasome impairment dependent increase of a monoubiquitinated form of MGRN1.
Title: Aging Triggers Cytoplasmic Depletion and Nuclear Translocation of the E3 Ligase Mahogunin: A Function for Ubiquitin in Neuronal Survival.
Findings suggest that mahogunin ring finger-1 contributes to the clearance of toxic mutant copper-zinc superoxide dismutase inclusions likely through autophagic pathway.
Title: Mahogunin ring finger 1 confers cytoprotection against mutant SOD1 aggresomes and is defective in an ALS mouse model.
Mahogunin-mediated alpha-tubulin ubiquitination via noncanonical K6 linkage regulates microtubule stability and mitotic spindle orientation.
Title: Mahogunin-mediated α-tubulin ubiquitination via noncanonical K6 linkage regulates microtubule stability and mitotic spindle orientation.
This study indicates the mahogunin deficiency results in the infertility of male mice, disruption of hormones secretion and impaired active progressive motility.
Title: The effect of mahogunin gene mutant on reproduction in male mice: a new sight for infertility?
ATRN also has no effect on prion disease onset or progression and discuss possible mechanisms that could cause vacuolation of the central nervous system in Mgrn1 and Atrn null mutant mice
Title: RML prions act through Mahogunin and Attractin-independent pathways.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Spontaneous (5)
Chemically induced (other) (1)
Gene trapped (1) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH125074 (e-PCR)
- UniSTS:161896

NoName (e-PCR)
- UniSTS:548544

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in endosome to lysosome transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in endosome to lysosome transport	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cAMP-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of cAMP-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of smoothened signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein monoubiquitination	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein polyubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in early endosome	IBA Inferred from Biological aspect of Ancestor more info
located_in early endosome	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase MGRN1

Names: RING-type E3 ubiquitin transferase MGRN1; mahoganoid; mahogunin RING finger protein 1; probable E3 ubiquitin-protein ligase MGRN1

NP_001239366.1

EC 2.3.2.27

NP_001343990.1

EC 2.3.2.27

NP_001343991.1

EC 2.3.2.27

NP_001365941.1

EC 2.3.2.27

NP_001365942.1

EC 2.3.2.27

NP_001365943.1

EC 2.3.2.27

NP_001365944.1

EC 2.3.2.27

NP_083933.1

EC 2.3.2.27

XP_006521900.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001252437.1 → NP_001239366.1 E3 ubiquitin-protein ligase MGRN1 isoform 1

See identical proteins and their annotated locations for NP_001239366.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes isoform (1).

Source sequence(s)

AC167018, AK153407

Consensus CDS

CCDS57016.1

UniProtKB/Swiss-Prot

Q9D074

Related

ENSMUSP00000155034.2, ENSMUST00000229038.2

Conserved Domains (3) summary

PHA02929
Location:233 → 332

PHA02929; N1R/p28-like protein; Provisional

TIGR00599
Location:274 → 494

rad18; DNA repair protein rad18

pfam13920
Location:278 → 320

zf-C3HC4_3; Zinc finger, C3HC4 type (RING finger)
NM_001357061.1 → NP_001343990.1 E3 ubiquitin-protein ligase MGRN1 isoform 3

Status: VALIDATED

Source sequence(s)

AC167018

Consensus CDS

CCDS88869.1

Related

ENSMUSP00000023159.10, ENSMUST00000023159.10

Conserved Domains (3) summary

cd16789
Location:277 → 317

mRING-HC-C3HC5_MGRN1_like---blasttree; Modified RING finger, HC subclass (C3HC5-type), found in mahogunin RING finger protein 1 (MGRN1), RING finger protein 157 (RNF157) and similar proteins

cl27165
Location:232 → 331

PHA02929; N1R/p28-like protein; Provisional

cl28410
Location:273 → 493

RAD18; RING-finger-containing E3 ubiquitin ligase [Signal transduction mechanisms]
NM_001357062.1 → NP_001343991.1 E3 ubiquitin-protein ligase MGRN1 isoform 4

Status: VALIDATED

Source sequence(s)

AC167018

Consensus CDS

CCDS88868.1

Related

ENSMUSP00000155425.2, ENSMUST00000230990.2

Conserved Domains (2) summary

cd16789
Location:277 → 317

mRING-HC-C3HC5_MGRN1_like---blasttree; Modified RING finger, HC subclass (C3HC5-type), found in mahogunin RING finger protein 1 (MGRN1), RING finger protein 157 (RNF157) and similar proteins

cl27165
Location:232 → 331

PHA02929; N1R/p28-like protein; Provisional
NM_001379012.1 → NP_001365941.1 E3 ubiquitin-protein ligase MGRN1 isoform 5

Status: VALIDATED

Source sequence(s)

AC167018

Conserved Domains (2) summary

cd16789
Location:277 → 317

mRING-HC-C3HC5_MGRN1_like---blasttree; Modified RING finger, HC subclass (C3HC5-type), found in mahogunin RING finger protein 1 (MGRN1), RING finger protein 157 (RNF157) and similar proteins

cl29320
Location:232 → 331

KilA-N; KilA-N domain
NM_001379013.1 → NP_001365942.1 E3 ubiquitin-protein ligase MGRN1 isoform 6

Status: VALIDATED

Source sequence(s)

AC167018

Conserved Domains (3) summary

TIGR00599
Location:274 → 494

rad18; DNA repair protein rad18

cd16789
Location:278 → 318

mRING-HC-C3HC5_MGRN1_like---blasttree; Modified RING finger, HC subclass (C3HC5-type), found in mahogunin RING finger protein 1 (MGRN1), RING finger protein 157 (RNF157) and similar proteins

cl29320
Location:233 → 332

KilA-N; KilA-N domain
NM_001379014.1 → NP_001365943.1 E3 ubiquitin-protein ligase MGRN1 isoform 7

Status: VALIDATED

Source sequence(s)

AC167018

Conserved Domains (2) summary

cd16789
Location:278 → 318

mRING-HC-C3HC5_MGRN1_like---blasttree; Modified RING finger, HC subclass (C3HC5-type), found in mahogunin RING finger protein 1 (MGRN1), RING finger protein 157 (RNF157) and similar proteins

cl29320
Location:233 → 332

KilA-N; KilA-N domain
NM_001379015.1 → NP_001365944.1 E3 ubiquitin-protein ligase MGRN1 isoform 2

Status: VALIDATED

Source sequence(s)

AC167018

UniProtKB/Swiss-Prot

Q3U5V9, Q3UDA1, Q6ZQ97, Q8BZM9, Q9D074

Conserved Domains (3) summary

TIGR00599
Location:273 → 493

rad18; DNA repair protein rad18

cd16789
Location:277 → 317

mRING-HC-C3HC5_MGRN1_like---blasttree; Modified RING finger, HC subclass (C3HC5-type), found in mahogunin RING finger protein 1 (MGRN1), RING finger protein 157 (RNF157) and similar proteins

cl29320
Location:232 → 331

KilA-N; KilA-N domain
NM_029657.4 → NP_083933.1 E3 ubiquitin-protein ligase MGRN1 isoform 2

See identical proteins and their annotated locations for NP_083933.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

Source sequence(s)

AC167018, AK011747

Consensus CDS

CCDS49751.1

UniProtKB/Swiss-Prot

Q3U5V9, Q3UDA1, Q6ZQ97, Q8BZM9, Q9D074

Related

ENSMUSP00000068314.9, ENSMUST00000070658.16

Conserved Domains (3) summary

TIGR00599
Location:273 → 493

rad18; DNA repair protein rad18

cd16789
Location:277 → 317

mRING-HC-C3HC5_MGRN1_like---blasttree; Modified RING finger, HC subclass (C3HC5-type), found in mahogunin RING finger protein 1 (MGRN1), RING finger protein 157 (RNF157) and similar proteins

cl29320
Location:232 → 331

KilA-N; KilA-N domain

RNA

NR_166412.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC167018

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000082.7 Reference GRCm39 C57BL/6J

Range

4703964..4756160

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006521837.1 → XP_006521900.1 E3 ubiquitin-protein ligase MGRN1 isoform X1

Conserved Domains (2) summary

cd16789
Location:278 → 318

mRING-HC-C3HC5_MGRN1_like---blasttree; Modified RING finger, HC subclass (C3HC5-type), found in mahogunin RING finger protein 1 (MGRN1), RING finger protein 157 (RNF157) and similar proteins

cl29320
Location:233 → 332

KilA-N; KilA-N domain