U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Smad3 SMAD family member 3 [ Mus musculus (house mouse) ]

Gene ID: 17127, updated on 19-Feb-2024

Summary

Official Symbol
Smad3provided by MGI
Official Full Name
SMAD family member 3provided by MGI
Primary source
MGI:MGI:1201674
See related
Ensembl:ENSMUSG00000032402 AllianceGenome:MGI:1201674
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Madh3
Summary
Enables several functions, including DNA binding activity; DNA-binding transcription activator activity, RNA polymerase II-specific; and promoter-specific chromatin binding activity. Involved in several processes, including JNK cascade; negative regulation of osteoblast proliferation; and positive regulation of chondrocyte differentiation. Acts upstream of or within several processes, including animal organ development; regulation of transcription by RNA polymerase II; and transmembrane receptor protein serine/threonine kinase signaling pathway. Located in cytoplasm; nucleus; and plasma membrane. Is expressed in several structures, including alimentary system; early conceptus; genitourinary system; limb; and nervous system. Used to study osteoarthritis. Human ortholog(s) of this gene implicated in Loeys-Dietz syndrome 3; Lynch syndrome; and pancreatic cancer. Orthologous to human SMAD3 (SMAD family member 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in ovary adult (RPKM 18.8), limb E14.5 (RPKM 18.2) and 28 other tissues See more
Orthologs
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

See Smad3 in Genome Data Viewer
Location:
9 C; 9 34.22 cM
Exon count:
11
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 9 NC_000075.7 (63554048..63665276, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 9 NC_000075.6 (63646766..63757994, complement)

Chromosome 9 - NC_000075.7Genomic Context describing neighboring genes Neighboring gene IQ motif containing H Neighboring gene predicted gene, 39358 Neighboring gene CapStarr-seq enhancer MGSCv37_chr9:63458940-63459049 Neighboring gene alpha- and gamma-adaptin binding protein Neighboring gene STARR-positive B cell enhancer ABC_E6753 Neighboring gene CapStarr-seq enhancer MGSCv37_chr9:63568712-63568904 Neighboring gene CapStarr-seq enhancer MGSCv37_chr9:63585488-63585725 Neighboring gene STARR-seq mESC enhancer starr_24405 Neighboring gene predicted gene, 51710 Neighboring gene predicted gene, 35941 Neighboring gene predicted gene, 39359

Genomic regions, transcripts, and products

Expression

  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Variation

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (5)  1 citation
  • Endonuclease-mediated (5) 

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables DEAD/H-box RNA helicase binding ISO
Inferred from Sequence Orthology
more info
 
enables DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
enables DNA binding ISO
Inferred from Sequence Orthology
more info
 
enables DNA-binding transcription activator activity, RNA polymerase II-specific IPI
Inferred from Physical Interaction
more info
PubMed 
enables DNA-binding transcription activator activity, RNA polymerase II-specific ISO
Inferred from Sequence Orthology
more info
 
enables DNA-binding transcription factor activity IDA
Inferred from Direct Assay
more info
PubMed 
contributes_to DNA-binding transcription factor activity ISO
Inferred from Sequence Orthology
more info
 
enables DNA-binding transcription factor activity ISO
Inferred from Sequence Orthology
more info
 
enables DNA-binding transcription factor activity, RNA polymerase II-specific IBA
Inferred from Biological aspect of Ancestor
more info
 
enables DNA-binding transcription factor activity, RNA polymerase II-specific ISO
Inferred from Sequence Orthology
more info
 
enables DNA-binding transcription factor binding ISO
Inferred from Sequence Orthology
more info
 
enables DNA-binding transcription repressor activity ISO
Inferred from Sequence Orthology
more info
 
enables I-SMAD binding IBA
Inferred from Biological aspect of Ancestor
more info
 
enables R-SMAD binding ISO
Inferred from Sequence Orthology
more info
 
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
 
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding ISO
Inferred from Sequence Orthology
more info
 
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding ISO
Inferred from Sequence Orthology
more info
 
enables RNA polymerase II-specific DNA-binding transcription factor binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables RNA polymerase II-specific DNA-binding transcription factor binding ISO
Inferred from Sequence Orthology
more info
 
enables bHLH transcription factor binding ISO
Inferred from Sequence Orthology
more info
 
enables beta-catenin binding ISO
Inferred from Sequence Orthology
more info
 
enables chromatin DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
enables chromatin binding IDA
Inferred from Direct Assay
more info
PubMed 
enables cis-regulatory region sequence-specific DNA binding ISO
Inferred from Sequence Orthology
more info
 
enables co-SMAD binding ISO
Inferred from Sequence Orthology
more info
 
enables collagen binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables double-stranded DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
enables enzyme binding ISO
Inferred from Sequence Orthology
more info
 
enables identical protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
 
enables nuclear glucocorticoid receptor binding ISO
Inferred from Sequence Orthology
more info
 
enables nuclear mineralocorticoid receptor binding ISO
Inferred from Sequence Orthology
more info
 
enables nuclear receptor binding ISO
Inferred from Sequence Orthology
more info
 
enables phosphatase binding ISO
Inferred from Sequence Orthology
more info
 
enables promoter-specific chromatin binding IDA
Inferred from Direct Assay
more info
PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables protein homodimerization activity ISO
Inferred from Sequence Orthology
more info
 
enables protein kinase binding ISO
Inferred from Sequence Orthology
more info
 
enables sequence-specific DNA binding ISO
Inferred from Sequence Orthology
more info
 
enables sterol response element binding ISO
Inferred from Sequence Orthology
more info
 
enables transcription cis-regulatory region binding ISO
Inferred from Sequence Orthology
more info
 
enables transcription coactivator binding ISO
Inferred from Sequence Orthology
more info
 
enables transcription corepressor binding ISO
Inferred from Sequence Orthology
more info
 
enables transforming growth factor beta receptor binding ISO
Inferred from Sequence Orthology
more info
 
enables ubiquitin binding ISO
Inferred from Sequence Orthology
more info
 
enables ubiquitin protein ligase binding ISO
Inferred from Sequence Orthology
more info
 
enables zinc ion binding ISO
Inferred from Sequence Orthology
more info
 
Process Evidence Code Pubs
involved_in JNK cascade IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in MAPK cascade IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in SMAD protein signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
 
acts_upstream_of_or_within SMAD protein signal transduction IGI
Inferred from Genetic Interaction
more info
PubMed 
acts_upstream_of SMAD protein signal transduction ISO
Inferred from Sequence Orthology
more info
 
involved_in SMAD protein signal transduction ISO
Inferred from Sequence Orthology
more info
 
involved_in T cell activation IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in activation of cysteine-type endopeptidase activity involved in apoptotic process ISO
Inferred from Sequence Orthology
more info
 
involved_in activin receptor signaling pathway IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in activin receptor signaling pathway ISO
Inferred from Sequence Orthology
more info
 
involved_in activin receptor signaling pathway NAS
Non-traceable Author Statement
more info
PubMed 
involved_in adrenal gland development ISO
Inferred from Sequence Orthology
more info
 
involved_in anatomical structure morphogenesis IBA
Inferred from Biological aspect of Ancestor
more info
 
acts_upstream_of_or_within apoptotic process IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in cell differentiation IBA
Inferred from Biological aspect of Ancestor
more info
 
acts_upstream_of_or_within cell population proliferation IGI
Inferred from Genetic Interaction
more info
PubMed 
involved_in cell-cell junction organization ISO
Inferred from Sequence Orthology
more info
 
involved_in cellular response to transforming growth factor beta stimulus ISO
Inferred from Sequence Orthology
more info
 
involved_in cellular response to virus IDA
Inferred from Direct Assay
more info
PubMed 
acts_upstream_of_or_within developmental growth IGI
Inferred from Genetic Interaction
more info
PubMed 
acts_upstream_of_or_within embryonic cranial skeleton morphogenesis IGI
Inferred from Genetic Interaction
more info
PubMed 
acts_upstream_of_or_within embryonic foregut morphogenesis IGI
Inferred from Genetic Interaction
more info
PubMed 
acts_upstream_of_or_within embryonic pattern specification IGI
Inferred from Genetic Interaction
more info
PubMed 
acts_upstream_of_or_within endoderm development IGI
Inferred from Genetic Interaction
more info
PubMed 
involved_in extrinsic apoptotic signaling pathway ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within gastrulation IGI
Inferred from Genetic Interaction
more info
PubMed 
acts_upstream_of_or_within heart looping IGI
Inferred from Genetic Interaction
more info
PubMed 
involved_in immune response ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within immune system development IGI
Inferred from Genetic Interaction
more info
PubMed 
acts_upstream_of_or_within in utero embryonic development IGI
Inferred from Genetic Interaction
more info
PubMed 
acts_upstream_of_or_within lens fiber cell differentiation IMP
Inferred from Mutant Phenotype
more info
PubMed 
acts_upstream_of_or_within liver development IGI
Inferred from Genetic Interaction
more info
PubMed 
acts_upstream_of_or_within mesoderm formation IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in negative regulation of apoptotic process ISO
Inferred from Sequence Orthology
more info
 
involved_in negative regulation of cardiac muscle hypertrophy in response to stress ISO
Inferred from Sequence Orthology
more info
 
involved_in negative regulation of cell growth ISO
Inferred from Sequence Orthology
more info
 
involved_in negative regulation of cell population proliferation ISO
Inferred from Sequence Orthology
more info
 
involved_in negative regulation of cytosolic calcium ion concentration ISO
Inferred from Sequence Orthology
more info
 
involved_in negative regulation of fat cell differentiation ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of negative regulation of gene expression ISO
Inferred from Sequence Orthology
more info
 
involved_in negative regulation of inflammatory response IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in negative regulation of lung blood pressure ISO
Inferred from Sequence Orthology
more info
 
involved_in negative regulation of miRNA transcription ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within negative regulation of osteoblast differentiation IGI
Inferred from Genetic Interaction
more info
PubMed 
involved_in negative regulation of osteoblast proliferation IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in negative regulation of protein catabolic process ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
PubMed 
involved_in negative regulation of transcription by RNA polymerase II ISO
Inferred from Sequence Orthology
more info
 
involved_in negative regulation of wound healing IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in nodal signaling pathway ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within osteoblast development IGI
Inferred from Genetic Interaction
more info
PubMed 
involved_in osteoblast differentiation IMP
Inferred from Mutant Phenotype
more info
PubMed 
acts_upstream_of_or_within paraxial mesoderm morphogenesis IMP
Inferred from Mutant Phenotype
more info
PubMed 
acts_upstream_of_or_within pericardium development IGI
Inferred from Genetic Interaction
more info
PubMed 
acts_upstream_of_or_within positive regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
PubMed 
involved_in positive regulation of DNA-templated transcription ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of bone mineralization ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of canonical Wnt signaling pathway ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of cell migration ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of chondrocyte differentiation IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in positive regulation of epithelial to mesenchymal transition ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of positive regulation of extracellular matrix assembly ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of focal adhesion assembly ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of gene expression ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of interleukin-1 beta production ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of positive regulation of miRNA transcription ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of nitric oxide biosynthetic process ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of positive chemotaxis ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of stress fiber assembly ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
 
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
PubMed 
involved_in positive regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
PubMed 
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II IGI
Inferred from Genetic Interaction
more info
PubMed 
involved_in positive regulation of transcription by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in positive regulation of transcription by RNA polymerase II ISO
Inferred from Sequence Orthology
more info
 
involved_in positive regulation of transforming growth factor beta3 production ISO
Inferred from Sequence Orthology
more info
 
involved_in protein stabilization ISO
Inferred from Sequence Orthology
more info
 
involved_in regulation of DNA-templated transcription ISO
Inferred from Sequence Orthology
more info
 
involved_in regulation of DNA-templated transcription NAS
Non-traceable Author Statement
more info
PubMed 
acts_upstream_of_or_within regulation of binding IDA
Inferred from Direct Assay
more info
PubMed 
acts_upstream_of_or_within regulation of epithelial cell proliferation IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in regulation of immune response IMP
Inferred from Mutant Phenotype
more info
PubMed 
acts_upstream_of_or_within regulation of striated muscle tissue development IDA
Inferred from Direct Assay
more info
PubMed 
involved_in regulation of transcription by RNA polymerase II ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within regulation of transforming growth factor beta receptor signaling pathway IDA
Inferred from Direct Assay
more info
PubMed 
involved_in regulation of transforming growth factor beta receptor signaling pathway ISO
Inferred from Sequence Orthology
more info
 
involved_in regulation of transforming growth factor beta2 production ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of response to angiotensin ISO
Inferred from Sequence Orthology
more info
 
involved_in response to hypoxia ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within response to transforming growth factor beta IGI
Inferred from Genetic Interaction
more info
PubMed 
involved_in signal transduction involved in regulation of gene expression ISO
Inferred from Sequence Orthology
more info
 
acts_upstream_of_or_within skeletal system development IGI
Inferred from Genetic Interaction
more info
PubMed 
acts_upstream_of_or_within somitogenesis IMP
Inferred from Mutant Phenotype
more info
PubMed 
acts_upstream_of_or_within thyroid gland development IGI
Inferred from Genetic Interaction
more info
PubMed 
involved_in transdifferentiation ISO
Inferred from Sequence Orthology
more info
 
involved_in transforming growth factor beta receptor signaling pathway IBA
Inferred from Biological aspect of Ancestor
more info
 
acts_upstream_of_or_within transforming growth factor beta receptor signaling pathway IDA
Inferred from Direct Assay
more info
PubMed 
acts_upstream_of_or_within transforming growth factor beta receptor signaling pathway IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in transforming growth factor beta receptor signaling pathway ISO
Inferred from Sequence Orthology
more info
 
involved_in transforming growth factor beta receptor signaling pathway NAS
Non-traceable Author Statement
more info
PubMed 
involved_in ureteric bud development IEP
Inferred from Expression Pattern
more info
PubMed 
Component Evidence Code Pubs
part_of SMAD protein complex ISO
Inferred from Sequence Orthology
more info
 
part_of chromatin ISO
Inferred from Sequence Orthology
more info
 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
 
located_in cytosol ISO
Inferred from Sequence Orthology
more info
 
located_in cytosol TAS
Traceable Author Statement
more info
 
part_of heteromeric SMAD protein complex IBA
Inferred from Biological aspect of Ancestor
more info
 
part_of heteromeric SMAD protein complex ISO
Inferred from Sequence Orthology
more info
 
part_of heteromeric SMAD protein complex NAS
Non-traceable Author Statement
more info
PubMed 
located_in nuclear inner membrane ISO
Inferred from Sequence Orthology
more info
 
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
 
located_in nucleoplasm TAS
Traceable Author Statement
more info
 
located_in nucleus IDA
Inferred from Direct Assay
more info
PubMed 
located_in nucleus ISO
Inferred from Sequence Orthology
more info
 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
PubMed 
part_of receptor complex ISO
Inferred from Sequence Orthology
more info
 
part_of transcription regulator complex ISO
Inferred from Sequence Orthology
more info
 

General protein information

Preferred Names
mothers against decapentaplegic homolog 3
Names
MAD homolog 3
Smad 3
mMad3
mad3
mothers against DPP homolog 3

NCBI Reference Sequences (RefSeq)

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_016769.4NP_058049.3  mothers against decapentaplegic homolog 3

    See identical proteins and their annotated locations for NP_058049.3

    Status: VALIDATED

    Source sequence(s)
    AC158515, AK048626, AK083158
    Consensus CDS
    CCDS23272.1
    UniProtKB/Swiss-Prot
    Q8BUN5, Q8BX84
    UniProtKB/TrEMBL
    Q3V3E0
    Related
    ENSMUSP00000034973.4, ENSMUST00000034973.10
    Conserved Domains (2) summary
    cd10491
    Location:8132
    MH1_SMAD_2_3; N-terminal Mad Homology 1 (MH1) domain in SMAD2 and SMAD3
    cd10985
    Location:224414
    MH2_SMAD_2_3; C-terminal Mad Homology 2 (MH2) domain in SMAD2 and SMAD3

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000075.7 Reference GRCm39 C57BL/6J

    Range
    63554048..63665276 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006510819.4XP_006510882.3  mothers against decapentaplegic homolog 3 isoform X1

    UniProtKB/TrEMBL
    Q3V3E0
    Conserved Domains (2) summary
    cd10985
    Location:192382
    MH2_SMAD_2_3; C-terminal Mad Homology 2 (MH2) domain in SMAD2 and SMAD3
    cl00055
    Location:37100
    MH1; N-terminal Mad Homology 1 (MH1) domain
  2. XM_006510821.5XP_006510884.1  mothers against decapentaplegic homolog 3 isoform X2

    See identical proteins and their annotated locations for XP_006510884.1

    Conserved Domains (1) summary
    cd10985
    Location:29219
    MH2_SMAD_2_3; C-terminal Mad Homology 2 (MH2) domain in SMAD2 and SMAD3