Format

Send to:

Choose Destination

DHX8 DEAH-box helicase 8 [ Homo sapiens (human) ]

Gene ID: 1659, updated on 10-Oct-2019

Summary

Official Symbol
DHX8provided by HGNC
Official Full Name
DEAH-box helicase 8provided by HGNC
Primary source
HGNC:HGNC:2749
See related
Ensembl:ENSG00000067596 MIM:600396
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
DDX8; Dhr2; HRH1; PRP22; PRPF22
Summary
This gene is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
Expression
Ubiquitous expression in bone marrow (RPKM 10.3), testis (RPKM 7.7) and 25 other tissues See more
Orthologs

Genomic context

See DHX8 in Genome Data Viewer
Location:
17q21.31
Exon count:
27
Annotation release Status Assembly Chr Location
109.20190905 current GRCh38.p13 (GCF_000001405.39) 17 NC_000017.11 (43483865..43544799)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 17 NC_000017.10 (41561330..41604185)

Chromosome 17 - NC_000017.11Genomic Context describing neighboring genes Neighboring gene RNA, U6 small nuclear 971, pseudogene Neighboring gene ribosomal protein L29 pseudogene 31 Neighboring gene ETS variant transcription factor 4 Neighboring gene mesenchyme homeobox 1 Neighboring gene uncharacterized LOC107985085 Neighboring gene long intergenic non-protein coding RNA 2594

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
Knockdown of DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8) by siRNA inhibits HIV-1 replication in CD4+/CCR5+/CXCR4+ TZM-bl HeLa cells PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

  • Spliceosome, organism-specific biosystem (from KEGG)
    Spliceosome, organism-specific biosystemAfter transcription, eukaryotic mRNA precursors contain protein-coding exons and noncoding introns. In the following splicing, introns are excised and exons are joined by a macromolecular complex, th...
  • Spliceosome, conserved biosystem (from KEGG)
    Spliceosome, conserved biosystemAfter transcription, eukaryotic mRNA precursors contain protein-coding exons and noncoding introns. In the following splicing, introns are excised and exons are joined by a macromolecular complex, th...
  • mRNA Processing, organism-specific biosystem (from WikiPathways)
    mRNA Processing, organism-specific biosystemThis process describes the conversion of precursor messenger RNA into mature messenger RNA (mRNA). The pre-mRNA molecule undergoes three main modifications. These modifications are 5' capping, 3' po...

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding IEA
Inferred from Electronic Annotation
more info
 
RNA binding HDA PubMed 
RNA binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
RNA helicase activity IEA
Inferred from Electronic Annotation
more info
 
identical protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
Process Evidence Code Pubs
RNA processing TAS
Traceable Author Statement
more info
PubMed 
RNA splicing TAS
Traceable Author Statement
more info
PubMed 
mRNA splicing, via spliceosome IC
Inferred by Curator
more info
PubMed 
mRNA splicing, via spliceosome IDA
Inferred from Direct Assay
more info
PubMed 
spliceosomal complex disassembly IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
Component Evidence Code Pubs
U2-type catalytic step 2 spliceosome IDA
Inferred from Direct Assay
more info
PubMed 
catalytic step 2 spliceosome IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
catalytic step 2 spliceosome IDA
Inferred from Direct Assay
more info
PubMed 
nuclear body IDA
Inferred from Direct Assay
more info
 
nucleoplasm IDA
Inferred from Direct Assay
more info
 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 
spliceosomal complex TAS
Traceable Author Statement
more info
PubMed 

General protein information

Preferred Names
ATP-dependent RNA helicase DHX8
Names
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase)
DEAH (Asp-Glu-Ala-His) box polypeptide 8
DEAH box protein 8
RNA helicase HRH1
NP_001289552.1
NP_001309145.1
NP_001309146.1
NP_001309147.1
NP_001309148.1
NP_001309149.1
NP_001309150.1
NP_004932.1

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001302623.2NP_001289552.1  ATP-dependent RNA helicase DHX8 isoform 2

    See identical proteins and their annotated locations for NP_001289552.1

    Status: REVIEWED

    Source sequence(s)
    AC068675, AK303319, BC038223, BE890949, D50487
    Consensus CDS
    CCDS77040.1
    UniProtKB/Swiss-Prot
    Q14562
    UniProtKB/TrEMBL
    B7Z8F4, F5H658, Q05CV4
    Related
    ENSP00000437886.1, ENST00000540306.5
    Conserved Domains (3) summary
    COG1643
    Location:5571139
    HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]
    cd05684
    Location:265343
    S1_DHX8_helicase; S1_DHX8_helicase: The N-terminal S1 domain of human ATP-dependent RNA helicase DHX8, a DEAH (Asp-Glu-Ala-His) box polypeptide. The DEAH-box RNA helicases are thought to play key roles in pre-mRNA splicing and DHX8 facilitates nuclear export of spliced ...
    cl28253
    Location:263422
    RpsA; Ribosomal protein S1 [Translation, ribosomal structure and biogenesis]
  2. NM_001322216.1NP_001309145.1  ATP-dependent RNA helicase DHX8 isoform 3

    Status: REVIEWED

    Source sequence(s)
    AC068675, AC087650
    Conserved Domains (6) summary
    smart00487
    Location:286471
    DEXDc; DEAD-like helicases superfamily
    smart00847
    Location:689771
    HA2; Helicase associated domain (HA2) Add an annotation
    cd00046
    Location:307445
    DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.
    cd00079
    Location:470627
    HELICc; Helicase superfamily c-terminal domain; associated with DEXDc-, DEAD-, and DEAH-box proteins, yeast initiation factor 4A, Ski2p, and Hepatitis C virus NS3 helicases; this domain is found in a wide variety of helicases and helicase related proteins; may ...
    cd05684
    Location:168
    S1_DHX8_helicase; S1_DHX8_helicase: The N-terminal S1 domain of human ATP-dependent RNA helicase DHX8, a DEAH (Asp-Glu-Ala-His) box polypeptide. The DEAH-box RNA helicases are thought to play key roles in pre-mRNA splicing and DHX8 facilitates nuclear export of spliced ...
    pfam07717
    Location:805904
    OB_NTP_bind; Oligonucleotide/oligosaccharide-binding (OB)-fold
  3. NM_001322217.1NP_001309146.1  ATP-dependent RNA helicase DHX8 isoform 4

    Status: REVIEWED

    Source sequence(s)
    AC068675, AC087650
    Conserved Domains (6) summary
    smart00487
    Location:470655
    DEXDc; DEAD-like helicases superfamily
    smart00847
    Location:873955
    HA2; Helicase associated domain (HA2) Add an annotation
    cd00046
    Location:491629
    DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.
    cd00079
    Location:654811
    HELICc; Helicase superfamily c-terminal domain; associated with DEXDc-, DEAD-, and DEAH-box proteins, yeast initiation factor 4A, Ski2p, and Hepatitis C virus NS3 helicases; this domain is found in a wide variety of helicases and helicase related proteins; may ...
    cd05684
    Location:174252
    S1_DHX8_helicase; S1_DHX8_helicase: The N-terminal S1 domain of human ATP-dependent RNA helicase DHX8, a DEAH (Asp-Glu-Ala-His) box polypeptide. The DEAH-box RNA helicases are thought to play key roles in pre-mRNA splicing and DHX8 facilitates nuclear export of spliced ...
    pfam07717
    Location:9891088
    OB_NTP_bind; Oligonucleotide/oligosaccharide-binding (OB)-fold
  4. NM_001322218.2NP_001309147.1  ATP-dependent RNA helicase DHX8 isoform 5

    Status: REVIEWED

    Source sequence(s)
    AC068675, AC087650
    Conserved Domains (6) summary
    smart00487
    Location:559744
    DEXDc; DEAD-like helicases superfamily
    smart00847
    Location:9621044
    HA2; Helicase associated domain (HA2) Add an annotation
    cd00046
    Location:580718
    DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.
    cd00079
    Location:743900
    HELICc; Helicase superfamily c-terminal domain; associated with DEXDc-, DEAD-, and DEAH-box proteins, yeast initiation factor 4A, Ski2p, and Hepatitis C virus NS3 helicases; this domain is found in a wide variety of helicases and helicase related proteins; may ...
    cd05684
    Location:263341
    S1_DHX8_helicase; S1_DHX8_helicase: The N-terminal S1 domain of human ATP-dependent RNA helicase DHX8, a DEAH (Asp-Glu-Ala-His) box polypeptide. The DEAH-box RNA helicases are thought to play key roles in pre-mRNA splicing and DHX8 facilitates nuclear export of spliced ...
    pfam07717
    Location:10781145
    OB_NTP_bind; Oligonucleotide/oligosaccharide-binding (OB)-fold
  5. NM_001322219.2NP_001309148.1  ATP-dependent RNA helicase DHX8 isoform 6

    Status: REVIEWED

    Source sequence(s)
    AC068675, AC087650
    Conserved Domains (6) summary
    smart00487
    Location:561746
    DEXDc; DEAD-like helicases superfamily
    smart00847
    Location:9641046
    HA2; Helicase associated domain (HA2) Add an annotation
    cd00046
    Location:582720
    DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.
    cd00079
    Location:745902
    HELICc; Helicase superfamily c-terminal domain; associated with DEXDc-, DEAD-, and DEAH-box proteins, yeast initiation factor 4A, Ski2p, and Hepatitis C virus NS3 helicases; this domain is found in a wide variety of helicases and helicase related proteins; may ...
    cd05684
    Location:265343
    S1_DHX8_helicase; S1_DHX8_helicase: The N-terminal S1 domain of human ATP-dependent RNA helicase DHX8, a DEAH (Asp-Glu-Ala-His) box polypeptide. The DEAH-box RNA helicases are thought to play key roles in pre-mRNA splicing and DHX8 facilitates nuclear export of spliced ...
    pfam07717
    Location:10801151
    OB_NTP_bind; Oligonucleotide/oligosaccharide-binding (OB)-fold
  6. NM_001322220.2NP_001309149.1  ATP-dependent RNA helicase DHX8 isoform 7

    Status: REVIEWED

    Source sequence(s)
    AC068675, AC087650
    Conserved Domains (6) summary
    smart00487
    Location:561746
    DEXDc; DEAD-like helicases superfamily
    smart00847
    Location:9641046
    HA2; Helicase associated domain (HA2) Add an annotation
    cd00046
    Location:582720
    DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.
    cd00079
    Location:745902
    HELICc; Helicase superfamily c-terminal domain; associated with DEXDc-, DEAD-, and DEAH-box proteins, yeast initiation factor 4A, Ski2p, and Hepatitis C virus NS3 helicases; this domain is found in a wide variety of helicases and helicase related proteins; may ...
    cd05684
    Location:265343
    S1_DHX8_helicase; S1_DHX8_helicase: The N-terminal S1 domain of human ATP-dependent RNA helicase DHX8, a DEAH (Asp-Glu-Ala-His) box polypeptide. The DEAH-box RNA helicases are thought to play key roles in pre-mRNA splicing and DHX8 facilitates nuclear export of spliced ...
    pfam07717
    Location:10801149
    OB_NTP_bind; Oligonucleotide/oligosaccharide-binding (OB)-fold
  7. NM_001322221.1NP_001309150.1  ATP-dependent RNA helicase DHX8 isoform 8

    Status: REVIEWED

    Source sequence(s)
    AC068675, AC087650
    Conserved Domains (3) summary
    COG1643
    Location:5551217
    HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]
    cd05684
    Location:263341
    S1_DHX8_helicase; S1_DHX8_helicase: The N-terminal S1 domain of human ATP-dependent RNA helicase DHX8, a DEAH (Asp-Glu-Ala-His) box polypeptide. The DEAH-box RNA helicases are thought to play key roles in pre-mRNA splicing and DHX8 facilitates nuclear export of spliced ...
    cl28253
    Location:261420
    RpsA; Ribosomal protein S1 [Translation, ribosomal structure and biogenesis]
  8. NM_004941.3NP_004932.1  ATP-dependent RNA helicase DHX8 isoform 1

    See identical proteins and their annotated locations for NP_004932.1

    Status: REVIEWED

    Description
    Transcript Variant: .
    Source sequence(s)
    AC068675, BC044586, D50487
    Consensus CDS
    CCDS11464.1
    UniProtKB/Swiss-Prot
    Q14562
    UniProtKB/TrEMBL
    Q05CV4, Q86YB2
    Related
    ENSP00000262415.2, ENST00000262415.8
    Conserved Domains (3) summary
    COG0539
    Location:263422
    RpsA; Ribosomal protein S1 [Translation, ribosomal structure and biogenesis]
    COG1643
    Location:5571219
    HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]
    cd05684
    Location:265343
    S1_DHX8_helicase; The N-terminal S1 domain of human ATP-dependent RNA helicase DHX8, a DEAH (Asp-Glu-Ala-His) box polypeptide. The DEAH-box RNA helicases are thought to play key roles in pre-mRNA splicing and DHX8 facilitates nuclear export of spliced mRNA by releasing ...

RNA

  1. NR_136225.1 RNA Sequence

    Status: REVIEWED

    Source sequence(s)
    AC068675, AC087650
  2. NR_136226.1 RNA Sequence

    Status: REVIEWED

    Source sequence(s)
    AC068675, AC087650
  3. NR_136227.1 RNA Sequence

    Status: REVIEWED

    Source sequence(s)
    AC068675, AC087650
  4. NR_136228.2 RNA Sequence

    Status: REVIEWED

    Source sequence(s)
    AC068675, AC087650

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109.20190905

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000017.11 Reference GRCh38.p13 Primary Assembly

    Range
    43483865..43544799
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Support Center