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pylS pyrrolysine--tRNA(Pyl) ligase [ Methanosarcina mazei Go1 ]

Gene ID: 1479787, updated on 3-Dec-2025
Gene symbol
pylS
Gene description
pyrrolysine--tRNA(Pyl) ligase
Locus tag
MM_RS07465
Gene type
protein coding
Organism
Methanosarcina mazei Go1 (strain: Go1, culture-collection: DSM:3647)
Lineage
Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group; Methanomicrobia; Methanosarcinales; Methanosarcinaceae; Methanosarcina
Old locus tag
MM_1445
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Sequence:
NC_003901.1 (1727617..1728981, complement)

NC_003901.1Genomic Context describing neighboring genes Neighboring gene 3-methylornithyl-N6-L-lysine dehydrogenase PylD Neighboring gene 3-methylornithine--L-lysine ligase PylC Neighboring gene methylornithine synthase PylB Neighboring gene hypothetical protein Neighboring gene prenyltransferase/squalene oxidase repeat-containing protein

Genomic Sequence:
NC_003901.1

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. A novel crystal form of pyrrolysyl-tRNA synthetase reveals the pre- and post-aminoacyl-tRNA synthesis conformational states of the adenylate and aminoacyl moieties and an asparagine residue in the catalytic site. Yanagisawa T, et al. Acta Crystallogr D Biol Crystallogr, 2013 Jan. PMID 23275158
  2. Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA. Srinivasan G, et al. Science, 2002 May 24. PMID 12029131
  3. The amber codon in the gene encoding the monomethylamine methyltransferase isolated from Methanosarcina barkeri is translated as a sense codon. James CM, et al. J Biol Chem, 2001 Sep 7. PMID 11435424
  4. Activation of the pyrrolysine suppressor tRNA requires formation of a ternary complex with class I and class II lysyl-tRNA synthetases. Polycarpo C, et al. Mol Cell, 2003 Aug. PMID 14536069
  5. An aminoacyl-tRNA synthetase that specifically activates pyrrolysine. Polycarpo C, et al. Proc Natl Acad Sci U S A, 2004 Aug 24. PMID 15314242, Free PMC Article

See all (7) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A novel crystal form contains two pyrrolysyl-tRNA synthetase dimers in the asymmetric unit, in which two subunits in one dimer each bind N-(tert-butyloxycarbonyl)-L-lysyladenylate (BocLys-AMP) and the two subunits in the other dimer each bind AMP.
    Title: A novel crystal form of pyrrolysyl-tRNA synthetase reveals the pre- and post-aminoacyl-tRNA synthesis conformational states of the adenylate and aminoacyl moieties and an asparagine residue in the catalytic site.
Submit: New GeneRIF Correction
Preferred Names
pyrrolysine--tRNA(Pyl) ligase
WP_011033391.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003901.1 Reference assembly

    Range
    1727617..1728981 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. WP_011033391.1 pyrrolysine--tRNA(Pyl) ligase

    See identical proteins and their annotated locations for WP_011033391.1

    UniProtKB/Swiss-Prot
    Q8PWY1
    UniProtKB/TrEMBL
    A0A0E3LT83, A0A0E3PZI8, A0A0E3RUJ0, A0A0E3RZQ6, A0A0E3WPC9, A0A0F8GPG2
    Conserved Domains (1) summary
    PRK09537
    Location:1 → 454
    pylS; pyrrolysine--tRNA(Pyl) ligase
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8PWY1.1

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