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Gcnt1 glucosaminyl (N-acetyl) transferase 1, core 2 [ Mus musculus (house mouse) ]

Gene ID: 14537, updated on 24-Jan-2023

Summary

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Official Symbol
Gcnt1provided by MGI
Official Full Name
glucosaminyl (N-acetyl) transferase 1, core 2provided by MGI
Primary source
MGI:MGI:95676
See related
Ensembl:ENSMUSG00000038843 AllianceGenome:MGI:95676
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
IGnT; C2GNT; C2GnT-L; C2GlcNAcT; B130048E03; C2 GlcNAcT; 5630400D21Rik
Summary
Predicted to enable beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity. Acts upstream of or within kidney morphogenesis and tissue morphogenesis. Predicted to be located in Golgi cisterna; extracellular space; and trans-Golgi network. Is expressed in several structures, including alimentary system; genitourinary system; nervous system; sensory organ; and skeleton. Orthologous to human GCNT1 (glucosaminyl (N-acetyl) transferase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in kidney adult (RPKM 36.0), liver E14 (RPKM 9.3) and 9 other tissues See more
Orthologs
human all
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Genomic context

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See Gcnt1 in Genome Data Viewer
Location:
19 B; 19 12.75 cM
Exon count:
9
Annotation release Status Assembly Chr Location
109 current GRCm39 (GCF_000001635.27) 19 NC_000085.7 (17303505..17350208, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 19 NC_000085.6 (17326141..17372844, complement)

Chromosome 19 - NC_000085.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 32341 Neighboring gene prune homolog 2 Neighboring gene riboflavin kinase Neighboring gene proprotein convertase subtilisin/kexin type 5 Neighboring gene predicted gene, 41813

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Deficiency in the glycosyltransferase Gcnt1 increases susceptibility to tuberculosis through a mechanism involving neutrophils. Fonseca KL, et al. Mucosal Immunol, 2020 Sep. PMID 32203062, Free PMC Article
  2. Core 2 ß1,6-N-acetylglucosaminyltransferase-I, crucial for P-selectin ligand expression is controlled by a distal enhancer regulated by STAT4 and T-bet in CD4+ T helper cells 1. Mardahl M, et al. Mol Immunol, 2016 Sep. PMID 27505708
  3. X-ray crystal structure of leukocyte type core 2 beta1,6-N-acetylglucosaminyltransferase. Evidence for a convergence of metal ion-independent glycosyltransferase mechanism. Pak JE, et al. J Biol Chem, 2006 Sep 8. PMID 16829524
  4. Core 2 oligosaccharides mediate eosinophil and neutrophil peritoneal but not lung recruitment. Broide DH, et al. Am J Physiol Lung Cell Mol Physiol, 2002 Feb. PMID 11792630
  5. Severe impairment of leukocyte rolling in venules of core 2 glucosaminyltransferase-deficient mice. Sperandio M, et al. Blood, 2001 Jun 15. PMID 11389021

See all (71) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Deficiency in the glycosyltransferase Gcnt1 increases susceptibility to tuberculosis through a mechanism involving neutrophils.
    Title: Deficiency in the glycosyltransferase Gcnt1 increases susceptibility to tuberculosis through a mechanism involving neutrophils.
  2. we show that Gcnt1 transcription and subsequent P-lselectin ligand induction in Th1 cells is governed by binding of STAT4 and T-bet to a distal enhancer and further regulated by epigenetic marks such as H3K27me3.
    Title: Core 2 ß1,6-N-acetylglucosaminyltransferase-I, crucial for P-selectin ligand expression is controlled by a distal enhancer regulated by STAT4 and T-bet in CD4+ T helper cells 1.
  3. simultaneous deletion of the Core2 beta1,6-N-acetylglucosaminyltransferase (C2GnT) enzymes C2GnT1 and C2Gnt2 resulted in delayed expulsion of worms.
    Title: Requirement for core 2 O-glycans for optimal resistance to helminth infection.
  4. striking differences between the requirement of FucT-VII and C2GlcNAcT-I for Ligands for E-selectin and P-selectin expression in CD4+ T cells.
    Title: Differential regulation and impact of fucosyltransferase VII and core 2 β1,6-N-acetyl-glycosaminyltransferase for generation of E-selectin and P-selectin ligands in murine CD4+ T cells.
  5. Core2 O-glycan structure is essential for expression of SI and DDP-IV during intestinal cell differentiation.
    Title: Core2 O-glycan structure is essential for the cell surface expression of sucrase isomaltase and dipeptidyl peptidase-IV during intestinal cell differentiation.
  6. Core2 1-6-N-glucosaminyltransferase-I deficiency protects injured arteries from neointima formation in ApoE-deficient mice
    Title: Core2 1-6-N-glucosaminyltransferase-I deficiency protects injured arteries from neointima formation in ApoE-deficient mice.
  7. Core2 1-6-N-glucosaminyltransferase-I is crucial for the formation of atherosclerotic lesions in apolipoprotein E-deficient mice.
    Title: Core2 1-6-N-glucosaminyltransferase-I is crucial for the formation of atherosclerotic lesions in apolipoprotein E-deficient mice.
  8. Pancreas carcinoma antigen, C2GnT, fused to invariant chain elicits T-cell response and tumor growth inhibition.
    Title: Pancreas carcinoma antigen fused to invariant chain elicits T-cell response and tumor growth inhibition.
  9. These results suggest that the core 2 GlcNAc extended glycan chains on megalin can change the ligand-binding affinity and capacity.
    Title: Core 2 GlcNAc modification and megalin ligand-binding activity.
  10. plays a critical role in lymphocyte trafficking during chronic inflammation
    Title: Core 2 branching beta1,6-N-acetylglucosaminyltransferase and high endothelial venule-restricted sulfotransferase collaboratively control lymphocyte homing.
Submit: New GeneRIF Correction See all GeneRIFs (14)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Gene trapped (1) 
  • Targeted (2)  1 citation
  • Endonuclease-mediated (2) 

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
enables beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity ISO
Inferred from Sequence Orthology
more info
  
enables glycosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in O-glycan processing, core 2 IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in O-glycan processing, core 2 ISO
Inferred from Sequence Orthology
more info
  
involved_in cell adhesion molecule production ISO
Inferred from Sequence Orthology
more info
  
involved_in glycoprotein biosynthetic process ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within kidney morphogenesis IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in leukocyte tethering or rolling ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of leukocyte tethering or rolling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within tissue morphogenesis IGI
Inferred from Genetic Interaction
more info
 PubMed 
Component Evidence Code Pubs
located_in Golgi apparatus IEA
Inferred from Electronic Annotation
more info
  
located_in Golgi cisterna ISO
Inferred from Sequence Orthology
more info
  
located_in extracellular space ISO
Inferred from Sequence Orthology
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in trans-Golgi network ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
Names
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase (Core 2 branching enzyme) (Core2-GlcNAc-transferase) (C2GNT)
core 2 beta-1,6-N-acetylglucosaminyltransferase
core 2-branching enzyme
core2-GlcNAc-transferase
leukocyte type core 2 beta-1,6-N-acetylglucosaminyltransferase
NP_001129956.3
NP_034395.4
NP_775618.3
XP_006526742.1
XP_011245446.1
XP_017173551.1
XP_036017326.1
XP_036017327.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001136484.3 → NP_001129956.3  beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

    See identical proteins and their annotated locations for NP_001129956.3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein.
    Source sequence(s)
    AC147369, BY431975, BY768580
    Consensus CDS
    CCDS29687.1
    UniProtKB/Swiss-Prot
    Q09324, Q8BRB2
    Related
    ENSMUSP00000127835.2, ENSMUST00000169897.2
    Conserved Domains (1) summary
    pfam02485
    Location:123 → 391
    Branch; Core-2/I-Branching enzyme

  2. NM_010265.5 → NP_034395.4  beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

    See identical proteins and their annotated locations for NP_034395.4

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein.
    Source sequence(s)
    AC147369, BY431975, CJ092264
    Consensus CDS
    CCDS29687.1
    UniProtKB/Swiss-Prot
    Q09324, Q8BRB2
    Related
    ENSMUSP00000157681.2, ENSMUST00000236139.2
    Conserved Domains (1) summary
    pfam02485
    Location:123 → 391
    Branch; Core-2/I-Branching enzyme

  3. NM_173442.5 → NP_775618.3  beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

    See identical proteins and their annotated locations for NP_775618.3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.
    Source sequence(s)
    AC147369, AK045216, BY431975
    Consensus CDS
    CCDS29687.1
    UniProtKB/Swiss-Prot
    Q09324, Q8BRB2
    Related
    ENSMUSP00000133935.3, ENSMUST00000174236.8
    Conserved Domains (1) summary
    pfam02485
    Location:123 → 391
    Branch; Core-2/I-Branching enzyme

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000085.7 Reference GRCm39 C57BL/6J

    Range
    17303505..17350208 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011247144.4 → XP_011245446.1  beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase isoform X1

    See identical proteins and their annotated locations for XP_011245446.1

    Conserved Domains (1) summary
    pfam02485
    Location:127 → 395
    Branch; Core-2/I-Branching enzyme

  2. XM_006526679.4 → XP_006526742.1  beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase isoform X1

    See identical proteins and their annotated locations for XP_006526742.1

    Conserved Domains (1) summary
    pfam02485
    Location:127 → 395
    Branch; Core-2/I-Branching enzyme

  3. XM_017318062.3 → XP_017173551.1  beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase isoform X1

    Conserved Domains (1) summary
    pfam02485
    Location:127 → 395
    Branch; Core-2/I-Branching enzyme

  4. XM_036161433.1 → XP_036017326.1  beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase isoform X1

    Conserved Domains (1) summary
    pfam02485
    Location:127 → 395
    Branch; Core-2/I-Branching enzyme

  5. XM_036161434.1 → XP_036017327.1  beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase isoform X1

    Related
    ENSMUSP00000157544.2, ENSMUST00000235184.2
    Conserved Domains (1) summary
    pfam02485
    Location:127 → 395
    Branch; Core-2/I-Branching enzyme
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q09324.2 GenPept UniProtKB/Swiss-Prot:Q09324

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