Sh3pxd2a SH3 and PX domains 2A [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Sh3pxd2aprovided by MGI
Official Full Name: SH3 and PX domains 2Aprovided by MGI
Primary source: MGI:MGI:1298393
See related: Ensembl:ENSMUSG00000053617 AllianceGenome:MGI:1298393
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Fish; TSK5; Tks5; Gm5098; Sh3md1; EG329070; C230050L11; 2310014D11Rik
Summary: Enables phosphatidylinositol phosphate binding activity; phosphatidylinositol-3,4-bisphosphate binding activity; and protease binding activity. Involved in reactive oxygen species metabolic process. Acts upstream of or within in utero embryonic development. Located in cytoplasm and podosome. Is expressed in several structures, including brain; clavicle; liver; skeletal muscle; and spleen. Orthologous to human SH3PXD2A (SH3 and PX domains 2A). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in subcutaneous fat pad adult (RPKM 33.7), mammary gland adult (RPKM 26.4) and 24 other tissues See more
Orthologs: human all
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Genomic context

Location:: 19; 19 C3

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
109	current	GRCm39 (GCF_000001635.27)	19	NC_000085.7 (47248613..47453053, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	19	NC_000085.6 (47260174..47464612, complement)

Chromosome 19 - NC_000085.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Tks5 Regulates Synaptic Podosome Formation and Stabilization of the Postsynaptic Machinery at the Neuromuscular Junction.
Title: Tks5 Regulates Synaptic Podosome Formation and Stabilization of the Postsynaptic Machinery at the Neuromuscular Junction.
Compressive force upregulated the expression of Ets-1 and Tks5.
Title: Orthodontic compressive force modulates Ets-1/Tks5 pathway to promote the formation of circumferential invadopodia and the fusion of osteoclast precursors.
Suggest the importance of Tks adaptor proteins in melanoma growth and metastasis in vivo is likely via functional invadopodia formation.
Title: The role of Tks adaptor proteins in invadopodia formation, growth and metastasis of melanoma.
This is the first study that identifies a new Rab40b-Tks5- and miR-204-dependent invadopodia transport pathway that regulates MMP2 and MMP9 secretion, and extracellular matrix remodeling during cancer progression.
Title: Rab40b and Tks5 interact at invadopodia during metastasis.
Results show that TKS5 is highly expressed in many cancer types with strong correlation with increased metastatic events and a poorer prognosis suggesting a clinical importance.
Title: Expression of the adaptor protein Tks5 in human cancer: prognostic potential.
Signaling leading to Tks5 activation in peritoneal mesothelial cells may be a suitable therapeutic target for prevention of peritoneal carcinomatosis.
Title: Tks5 activation in mesothelial cells creates invasion front of peritoneal carcinomatosis.
Tks5 undergoes an isoform switch during metastatic progression in a genetically engineered mouse model of lung adenocarcinoma
Title: Differential Tks5 isoform expression contributes to metastatic invasion of lung adenocarcinoma.
Tks5, a master regulator of invadopodia in cancer cells, is crucial for osteoclast fusion downstream of phosphoinositide 3-kinase and Src.
Title: Tks5-dependent formation of circumferential podosomes/invadopodia mediates cell-cell fusion.
Src-Tks5 pathway is required for neural crest cell migration during embryonic development
Title: A Src-Tks5 pathway is required for neural crest cell migration during embryonic development.
Tks5 facilitates the production of ROS necessary for invadopodia formation, and in turn ROS modulate Tks5 tyrosine phosphorylation in a positive feedback loop.
Title: Tks5-dependent, nox-mediated generation of reactive oxygen species is necessary for invadopodia formation.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI413738 (e-PCR)
- UniSTS:181253

RH124986 (e-PCR)
- UniSTS:161808

AU048745 (e-PCR)
- UniSTS:145195

AA589508 (e-PCR)
- UniSTS:160855

bnlg1246b (e-PCR), detects polymorphism
- UniSTS:472187

RH136079 (e-PCR)
- UniSTS:210074

Homology

Homologs of the Sh3pxd2a gene: The Sh3pxd2a gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, zebrafish, and frog.
Orthologs from Annotation Pipeline: 488 organisms have orthologs with human gene Sh3pxd2a
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables phosphatidylinositol binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphatidylinositol-3,4-bisphosphate binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphatidylinositol-3-phosphate binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphatidylinositol-4,5-bisphosphate binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphatidylinositol-4-phosphate binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphatidylinositol-5-phosphate binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protease binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables superoxide-generating NADPH oxidase activator activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within in utero embryonic development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in osteoclast fusion	ISO Inferred from Sequence Orthology more info
involved_in reactive oxygen species metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in superoxide anion generation	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in superoxide metabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cell junction	IEA Inferred from Electronic Annotation more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in podosome	IDA Inferred from Direct Assay more info	PubMed
located_in podosome	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: SH3 and PX domain-containing protein 2A

Names: SH3 multiple domains 1; SH3 multiple domains protein 1; five SH3 domain-containing protein; five SH3 domains; tyrosine kinase substrate with five SH3 domains

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001164717.2 → NP_001158189.1 SH3 and PX domain-containing protein 2A isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.

Source sequence(s)

AC132268, AC132288

Consensus CDS

CCDS50464.1

UniProtKB/Swiss-Prot

O89032

Related

ENSMUSP00000107430.3, ENSMUST00000111800.4

Conserved Domains (6) summary

cd06888
Location:6 → 124

PX_FISH; The phosphoinositide binding Phox Homology domain of Five SH protein

cd12019
Location:810 → 860

SH3_Tks5_4; Fourth Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

cd12020
Location:1039 → 1095

SH3_Tks5_5; Fifth (C-terminal) Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

cd12074
Location:170 → 222

SH3_Tks5_1; First Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

cd12077
Location:241 → 294

SH3_Tks5_2; Second Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

cd12079
Location:422 → 475

SH3_Tks5_3; Third Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
NM_008018.5 → NP_032044.2 SH3 and PX domain-containing protein 2A isoform 1

See identical proteins and their annotated locations for NP_032044.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC132268, AC132288

Consensus CDS

CCDS29891.1

UniProtKB/Swiss-Prot

O89032, Q148Q8

Related

ENSMUSP00000080325.3, ENSMUST00000081619.10

Conserved Domains (6) summary

cd06888
Location:6 → 124

PX_FISH; The phosphoinositide binding Phox Homology domain of Five SH protein

cd12019
Location:838 → 888

SH3_Tks5_4; Fourth Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

cd12020
Location:1067 → 1123

SH3_Tks5_5; Fifth (C-terminal) Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

cd12074
Location:170 → 222

SH3_Tks5_1; First Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

cd12077
Location:269 → 322

SH3_Tks5_2; Second Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

cd12079
Location:450 → 503

SH3_Tks5_3; Third Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000085.7 Reference GRCm39 C57BL/6J

Range

47248613..47453053 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036161432.1 → XP_036017325.1 SH3 and PX domain-containing protein 2A isoform X2

Conserved Domains (6) summary

cd06888
Location:6 → 124

PX_FISH; The phosphoinositide binding Phox Homology domain of Five SH protein

cd12019
Location:795 → 845

SH3_Tks5_4; Fourth Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

cd12020
Location:1024 → 1080

SH3_Tks5_5; Fifth (C-terminal) Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

cd12074
Location:155 → 207

SH3_Tks5_1; First Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

cd12077
Location:226 → 279

SH3_Tks5_2; Second Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

cd12079
Location:407 → 460

SH3_Tks5_3; Third Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
XM_006526669.5 → XP_006526732.1 SH3 and PX domain-containing protein 2A isoform X1

Conserved Domains (6) summary

cd06888
Location:6 → 124

PX_FISH; The phosphoinositide binding Phox Homology domain of Five SH protein

cd12019
Location:823 → 873

SH3_Tks5_4; Fourth Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

cd12020
Location:1052 → 1108

SH3_Tks5_5; Fifth (C-terminal) Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

cd12074
Location:155 → 207

SH3_Tks5_1; First Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

cd12077
Location:254 → 307

SH3_Tks5_2; Second Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

cd12079
Location:435 → 488

SH3_Tks5_3; Third Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
XM_006526670.5 → XP_006526733.1 SH3 and PX domain-containing protein 2A isoform X3

Conserved Domains (3) summary

cd12019
Location:368 → 418

SH3_Tks5_4; Fourth Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

cd12020
Location:597 → 653

SH3_Tks5_5; Fifth (C-terminal) Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

cl17036
Location:4 → 33

SH3; Src Homology 3 domain superfamily

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_177833.2: Suppressed sequence

Description

NM_177833.2: This RefSeq was permanently suppressed because currently there is support for the transcript but not for the protein.