Ctsl cathepsin L [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ctslprovided by MGI
Official Full Name: cathepsin Lprovided by MGI
Primary source: MGI:MGI:88564
See related: Ensembl:ENSMUSG00000021477 AllianceGenome:MGI:88564
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: fs; MEP; nkt; CatL; Ctsl1; 1190035F06Rik
Summary: This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the activation peptide and the cathepsin L1 heavy and light chains. The mature enzyme appears to be important in embryonic development through its processing of histone H3 and may play a role in disease progression in a model of kidney disease. Homozygous knockout mice for this gene exhibit hair loss, skin thickening, bone and heart defects, and enhanced susceptibility to bacterial infection. A pseudogene of this gene has been identified in the genome. [provided by RefSeq, Aug 2015]
Expression: Broad expression in placenta adult (RPKM 194.5), liver E18 (RPKM 75.5) and 20 other tissues See more
Orthologs: human all
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Genomic context

Location:: 13 B3; 13 33.26 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	13	NC_000079.7 (64509704..64518586, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	13	NC_000079.6 (64361890..64370772, complement)

Chromosome 13 - NC_000079.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cathepsin L was involved in vascular aging by mediating phenotypic transformation of vascular cells.
Title: Cathepsin L was involved in vascular aging by mediating phenotypic transformation of vascular cells.
Double deficiency of cathepsin B and L in the mouse pancreas alters trypsin activity without affecting acute pancreatitis severity.
Title: Double deficiency of cathepsin B and L in the mouse pancreas alters trypsin activity without affecting acute pancreatitis severity.
Cathepsin L, a Target of Hypoxia-Inducible Factor-1-alpha, Is Involved in Melanosome Degradation in Melanocytes.
Title: Cathepsin L, a Target of Hypoxia-Inducible Factor-1-α, Is Involved in Melanosome Degradation in Melanocytes.
Cathepsin L regulates pathogenicCD4 T cells in experimental autoimmune encephalomyelitis.
Title: Cathepsin L regulates pathogenicCD4 T cells in experimental autoimmune encephalomyelitis.
Sequential, but not Concurrent, Incubation of Cathepsin K and L with Type I Collagen Results in Extended Proteolysis.
Title: Sequential, but not Concurrent, Incubation of Cathepsin K and L with Type I Collagen Results in Extended Proteolysis.
This study reveals a previously undescribed molecular mechanism by which peripheral CPL-1/cathepsin L inhibition induces fat loss in C. elegans and mice through promoting central serotonin signaling.
Title: Peripheral cathepsin L inhibition induces fat loss in C. elegans and mice through promoting central serotonin synthesis.
serpin B13 inhibition and downstream targeting of E-cadherin by cathepsin L regulates cell proliferation in mouse and human pancreatic islets
Title: Cellular proliferation in mouse and human pancreatic islets is regulated by serpin B13 inhibition and downstream targeting of E-cadherin by cathepsin L.
Cts L regulates metabolic circuit to keep cell division under control.
Title: Cathepsin L Regulates Metabolic Networks Controlling Rapid Cell Growth and Proliferation.
High cathepsin L expression is associated with aortic aneurysms.
Title: Expression levels of cathepsin L and cystatin C in a hyperglycemic environment were associated with aortic aneurysm development in a mouse model.
These findings establish cathepsin L as a critical regulator of the inflammation that leads to intimal hyperplasia and that the TLR4- MyD88 pathway in myeloid lineages regulates cathepsin L expression in the vessel wall following wire injury.
Title: Cathepsin L promotes Vascular Intimal Hyperplasia after Arterial Injury.

Submit: New GeneRIF Correction See all GeneRIFs (95)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1)
Endonuclease-mediated (5)
Radiation induced (1) 1 citation
Spontaneous (1)
Targeted (7) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

MGI:6430 (e-PCR)
- UniSTS:141442

MGI:6402 (e-PCR)
- UniSTS:141443

Ctsl (e-PCR), detects polymorphism
- UniSTS:502679

Ctsl (e-PCR), detects polymorphism
- UniSTS:256969

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables aminopeptidase activity	ISO Inferred from Sequence Orthology more info
enables aminopeptidase activity	ISS Inferred from Sequence or Structural Similarity more info
enables collagen binding	ISO Inferred from Sequence Orthology more info
enables cysteine-type carboxypeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables cysteine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cysteine-type endopeptidase activity	IEA Inferred from Electronic Annotation more info
enables cysteine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info
enables cysteine-type endopeptidase activity	ISS Inferred from Sequence or Structural Similarity more info
enables cysteine-type peptidase activity	IEA Inferred from Electronic Annotation more info
enables cysteine-type peptidase activity	ISO Inferred from Sequence Orthology more info
enables endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables fibronectin binding	ISO Inferred from Sequence Orthology more info
enables histone binding	IDA Inferred from Direct Assay more info	PubMed
enables histone binding	ISO Inferred from Sequence Orthology more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables kininogen binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables peptidase activity	IEA Inferred from Electronic Annotation more info
enables peptide binding	ISO Inferred from Sequence Orthology more info
enables peptide binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables proteoglycan binding	ISO Inferred from Sequence Orthology more info
enables serpin family protein binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in CD4-positive, alpha-beta T cell lineage commitment	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Sertoli cell differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in antigen processing and presentation of peptide antigen	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in autophagic cell death	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to starvation	ISS Inferred from Sequence or Structural Similarity more info
involved_in collagen catabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within decidualization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in decidualization	ISS Inferred from Sequence or Structural Similarity more info
involved_in elastin catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in enkephalin processing	ISS Inferred from Sequence or Structural Similarity more info
NOT involved_in epithelial tube branching involved in lung morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within hair follicle morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within heparan sulfate proteoglycan catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in male gonad development	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within negative regulation of keratinocyte proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nerve development	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within protein autoprocessing	IDA Inferred from Direct Assay more info	PubMed
involved_in protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in proteolysis	IDA Inferred from Direct Assay more info	PubMed
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in proteolysis	ISO Inferred from Sequence Orthology more info
involved_in proteolysis	ISS Inferred from Sequence or Structural Similarity more info
involved_in proteolysis involved in protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteolysis involved in protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to glucocorticoid	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to glucose	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to gonadotropin	ISS Inferred from Sequence or Structural Similarity more info
involved_in spermatogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in thyroid hormone generation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in zymogen activation	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in apical part of cell	ISO Inferred from Sequence Orthology more info
colocalizes_with apical part of cell	ISS Inferred from Sequence or Structural Similarity more info
located_in apical plasma membrane	IEA Inferred from Electronic Annotation more info
located_in chromaffin granule	IEA Inferred from Electronic Annotation more info
located_in chromaffin granule	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasmic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in external side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in external side of plasma membrane	ISO Inferred from Sequence Orthology more info
located_in external side of plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in extracellular matrix	HDA more info	PubMed
located_in extracellular region	IEA Inferred from Electronic Annotation more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular space	ISO Inferred from Sequence Orthology more info
is_active_in lysosome	IBA Inferred from Biological aspect of Ancestor more info
located_in lysosome	IDA Inferred from Direct Assay more info	PubMed
located_in lysosome	IEA Inferred from Electronic Annotation more info
located_in lysosome	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in microvillus	ISO Inferred from Sequence Orthology more info
located_in microvillus	ISS Inferred from Sequence or Structural Similarity more info
located_in multivesicular body	ISO Inferred from Sequence Orthology more info
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in perikaryon	ISO Inferred from Sequence Orthology more info
located_in perikaryon	ISS Inferred from Sequence or Structural Similarity more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in secretory granule	ISO Inferred from Sequence Orthology more info
colocalizes_with secretory granule	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: procathepsin L

Names: Cat L; cathepsin L1; major excreted protein; p39 cysteine proteinase

NP_034114.1

EC 3.4.22.15

XP_006517143.1

EC 3.4.22.15

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001428550.1 → NP_001415479.1 procathepsin L isoform 2 precursor

Status: VALIDATED

Source sequence(s)

CT030159

UniProtKB/TrEMBL

Q3U5U9
NM_001428551.1 → NP_001415480.1 procathepsin L isoform 3 precursor

Status: VALIDATED

Source sequence(s)

CT030159

UniProtKB/TrEMBL

Q3TNC8
NM_001428552.1 → NP_001415481.1 procathepsin L isoform 4 precursor

Status: VALIDATED

Source sequence(s)

CT030159

UniProtKB/TrEMBL

Q3TVJ1
NM_001428553.1 → NP_001415482.1 procathepsin L isoform 5 precursor

Status: VALIDATED

Source sequence(s)

CT030159

UniProtKB/TrEMBL

Q3TT75
NM_001428554.1 → NP_001415483.1 procathepsin L isoform 6 precursor

Status: VALIDATED

Source sequence(s)

CT030159
NM_001428555.1 → NP_001415484.1 procathepsin L isoform 7 precursor

Status: VALIDATED

Source sequence(s)

CT030159

UniProtKB/TrEMBL

Q3TNC8, Q3TT75, Q3TVJ1, Q3U5U9, Q3UHZ4, Q9D0C0
NM_001428557.1 → NP_001415486.1 procathepsin L isoform 8 precursor

Status: VALIDATED

Source sequence(s)

CT030159

UniProtKB/TrEMBL

Q3TNC8, Q3TT75, Q3TVJ1, Q3U5U9, Q3UHZ4, Q9D0C0
NM_001428567.1 → NP_001415496.1 procathepsin L isoform 9

Status: VALIDATED

Source sequence(s)

CT030159

UniProtKB/TrEMBL

Q3UWH6
NM_001428568.1 → NP_001415497.1 procathepsin L isoform 9

Status: VALIDATED

Source sequence(s)

CT030159

UniProtKB/TrEMBL

Q3UWH6
NM_001428569.1 → NP_001415498.1 procathepsin L isoform 10 precursor

Status: VALIDATED

Source sequence(s)

CT030159

UniProtKB/TrEMBL

Q3U5U9, Q9D0C0
NM_001428570.1 → NP_001415499.1 procathepsin L isoform 11 precursor

Status: VALIDATED

Source sequence(s)

CT030159
NM_001428571.1 → NP_001415500.1 procathepsin L isoform 12 precursor

Status: VALIDATED

Source sequence(s)

CT030159
NM_001428572.1 → NP_001415501.1 procathepsin L isoform 13 precursor

Status: VALIDATED

Source sequence(s)

CT030159
NM_009984.5 → NP_034114.1 procathepsin L isoform 1 preproprotein

See identical proteins and their annotated locations for NP_034114.1

Status: VALIDATED

Source sequence(s)

CT030159

Consensus CDS

CCDS26600.1

UniProtKB/Swiss-Prot

P06797, Q91UZ0

UniProtKB/TrEMBL

Q3TNC8, Q3TT75, Q3TVJ1, Q3U5U9, Q3UHZ4, Q543M3, Q9D0C0

Related

ENSMUSP00000021933.8, ENSMUST00000021933.8

Conserved Domains (2) summary

smart00848
Location:29 → 87

Inhibitor_I29; Cathepsin propeptide inhibitor domain (I29)

pfam00112
Location:114 → 331

Peptidase_C1; Papain family cysteine protease