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MED8 mediator complex subunit 8 [ Homo sapiens (human) ]

Gene ID: 112950, updated on 4-Nov-2018

Summary

Official Symbol
MED8provided by HGNC
Official Full Name
mediator complex subunit 8provided by HGNC
Primary source
HGNC:HGNC:19971
See related
Ensembl:ENSG00000159479 MIM:607956; Vega:OTTHUMG00000007421
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
ARC32
Summary
This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
Expression
Ubiquitous expression in thyroid (RPKM 12.7), placenta (RPKM 11.6) and 25 other tissues See more
Orthologs

Genomic context

See MED8 in Genome Data Viewer
Location:
1p34.2
Exon count:
8
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) 1 NC_000001.11 (43383908..43389812, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (43849579..43855483, complement)

Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene ELOVL fatty acid elongase 1 Neighboring gene microRNA 6734 Neighboring gene uncharacterized LOC112268225 Neighboring gene uncharacterized LOC107984947 Neighboring gene SZT2, KICSTOR complex subunit Neighboring gene SZT2 antisense RNA 1 Neighboring gene microRNA 6735 Neighboring gene hydroxypyruvate isomerase (putative)

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Jun 15 11:32:44 2016

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
Knockdown of mediator complex subunit 8 (MED8) by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Tat tat Knockdown of MED8 by siRNAs inhibits HIV LTR-beta-gal activation in the Tat transactivation assay PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

  • Developmental Biology, organism-specific biosystem (from REACTOME)
    Developmental Biology, organism-specific biosystemAs a first step towards capturing the array of processes by which a fertilized egg gives rise to the diverse tissues of the body, examples of three kinds of processes have been annotated. These are a...
  • Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem (from REACTOME)
    Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystemThe reactions involved in the metabolism of fatty acids and of the triacylglycerols and ketone bodies derived from them form a closely interrelated, coordinately regulated module that plays a central...
  • Gene Expression, organism-specific biosystem (from REACTOME)
    Gene Expression, organism-specific biosystemGene Expression covers the pathways by which genomic DNA is transcribed to yield RNA, the regulation of these transcription processes, and the pathways by which newly-made RNA Transcripts are process...
  • Generic Transcription Pathway, organism-specific biosystem (from REACTOME)
    Generic Transcription Pathway, organism-specific biosystemOVERVIEW OF TRANSCRIPTION REGULATION: Detailed studies of gene transcription regulation in a wide variety of eukaryotic systems has revealed the general principles and mechanisms by which cell- or t...
  • Herpes simplex infection, organism-specific biosystem (from KEGG)
    Herpes simplex infection, organism-specific biosystemHerpes simplex virus (HSV) infections are very common worldwide, with the prevalence of HSV-1 reaching up to 80%-90%. Primary infection with HSV takes place in the mucosa, followed by the establishme...
  • Herpes simplex infection, conserved biosystem (from KEGG)
    Herpes simplex infection, conserved biosystemHerpes simplex virus (HSV) infections are very common worldwide, with the prevalence of HSV-1 reaching up to 80%-90%. Primary infection with HSV takes place in the mucosa, followed by the establishme...
  • Metabolism, organism-specific biosystem (from REACTOME)
    Metabolism, organism-specific biosystemMetabolic processes in human cells generate energy through the oxidation of molecules consumed in the diet and mediate the synthesis of diverse essential molecules not taken in the diet as well as th...
  • Metabolism of lipids and lipoproteins, organism-specific biosystem (from REACTOME)
    Metabolism of lipids and lipoproteins, organism-specific biosystemLipids are hydrophobic but otherwise chemically diverse molecules that play a wide variety of roles in human biology. They include ketone bodies, fatty acids, triacylglycerols, phospholipids and sphi...
  • PPARA activates gene expression, organism-specific biosystem (from REACTOME)
    PPARA activates gene expression, organism-specific biosystemThe set of genes regulated by PPAR-alpha is not fully known in humans, however many examples have been found in mice. Genes directly activated by PPAR-alpha contain peroxisome proliferator receptor e...
  • Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha), organism-specific biosystem (from REACTOME)
    Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha), organism-specific biosystemPeroxisome proliferator-activated receptor alpha (PPAR-alpha) is the major regulator of fatty acid oxidation in the liver. PPARalpha is also the target of fibrate drugs used to treat abnormal plasma ...
  • Transcriptional regulation of white adipocyte differentiation, organism-specific biosystem (from REACTOME)
    Transcriptional regulation of white adipocyte differentiation, organism-specific biosystemAdipogenesis is the process of cell differentiation by which preadipocytes become adipocytes. During this process the preadipocytes cease to proliferate, begin to accumulate lipid droplets and develo...

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Potential readthrough

Included gene: ELOVL1

Homology

Clone Names

  • MGC17544, MGC19641

Gene Ontology Provided by GOA

Function Evidence Code Pubs
RNA polymerase II proximal promoter sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
transcription coregulator activity IEA
Inferred from Electronic Annotation
more info
 
Process Evidence Code Pubs
protein ubiquitination IEA
Inferred from Electronic Annotation
more info
 
regulation of transcription by RNA polymerase II IEA
Inferred from Electronic Annotation
more info
 
transcription initiation from RNA polymerase II promoter TAS
Traceable Author Statement
more info
 
Component Evidence Code Pubs
core mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
mediator complex IDA
Inferred from Direct Assay
more info
PubMed 
nucleoplasm TAS
Traceable Author Statement
more info
 

General protein information

Preferred Names
mediator of RNA polymerase II transcription subunit 8
Names
activator-recruited cofactor 32 kDa component
mediator of RNA polymerase II transcription subunit MED8

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001001653.2NP_001001653.1  mediator of RNA polymerase II transcription subunit 8 isoform 1

    See identical proteins and their annotated locations for NP_001001653.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) contains multiple differences in the UTRs and coding region, compared to variant 3. It initiates translation at a downstream in-frame start codon. The encoded isoform (1) has shorter N- and C-termini, compared to isoform 3.
    Source sequence(s)
    AL139289, BC010019, BF196855, BG620658, CB162354
    Consensus CDS
    CCDS60108.1
    UniProtKB/Swiss-Prot
    Q96G25
    Related
    ENSP00000361533.4, OTTHUMP00000008573, ENST00000372455.4, OTTHUMT00000019491
    Conserved Domains (1) summary
    pfam10232
    Location:1176
    Med8; Mediator of RNA polymerase II transcription complex subunit 8
  2. NM_052877.4NP_443109.2  mediator of RNA polymerase II transcription subunit 8 isoform 3

    See identical proteins and their annotated locations for NP_443109.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) represents the shortest transcript and encodes the longest isoform (3).
    Source sequence(s)
    BC010543, BF196855, BG620658, BI488866
    Consensus CDS
    CCDS486.2
    UniProtKB/Swiss-Prot
    Q96G25
    Related
    ENSP00000290663.6, OTTHUMP00000008581, ENST00000290663.10, OTTHUMT00000019520
    Conserved Domains (1) summary
    pfam10232
    Location:1265
    Med8; Mediator of RNA polymerase II transcription complex subunit 8
  3. NM_201542.4NP_963836.2  mediator of RNA polymerase II transcription subunit 8 isoform 4

    See identical proteins and their annotated locations for NP_963836.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (5) uses an alternate exon structure in the 3' coding region and differs in the 3' UTR, compared to variant 3. The resulting isoform (4) has a shorter C-terminus, compared to isoform 3.
    Source sequence(s)
    AF521562, AL139289, BC010019, BF196855, BG620658, CB162354
    Consensus CDS
    CCDS487.2
    UniProtKB/Swiss-Prot
    Q96G25
    Related
    ENSP00000361535.4, OTTHUMP00000197861, ENST00000372457.8, OTTHUMT00000318959
    Conserved Domains (1) summary
    pfam10232
    Location:1265
    Med8; Mediator of RNA polymerase II transcription complex subunit 8

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000001.11 Reference GRCh38.p12 Primary Assembly

    Range
    43383908..43389812 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
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