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CHKA choline kinase alpha [ Homo sapiens (human) ]

Gene ID: 1119, updated on 3-Oct-2020

Summary

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Official Symbol
CHKAprovided by HGNC
Official Full Name
choline kinase alphaprovided by HGNC
Primary source
HGNC:HGNC:1937
See related
Ensembl:ENSG00000110721 MIM:118491
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
CK; EK; CHK; CKI
Summary
The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Expression
Broad expression in testis (RPKM 24.3), thyroid (RPKM 9.0) and 25 other tissues See more
Orthologs
mouse all

Genomic context

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See CHKA in Genome Data Viewer
Location:
11q13.2
Exon count:
14
Annotation release Status Assembly Chr Location
109.20200815 current GRCh38.p13 (GCF_000001405.39) 11 NC_000011.10 (68052859..68121389, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 11 NC_000011.9 (67820326..67888858, complement)

Chromosome 11 - NC_000011.10Genomic Context describing neighboring genes Neighboring gene NADH:ubiquinone oxidoreductase core subunit S8 Neighboring gene microRNA 4691 Neighboring gene T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 Neighboring gene microRNA 6753 Neighboring gene uncharacterized LOC105369362 Neighboring gene lysine methyltransferase 5B

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Identification of a Unique Inhibitor-Binding Site on Choline Kinase α. Kall SL, et al. Biochemistry, 2018 Feb 27. PMID 29389115
  2. Choline Kinase α Mediates Interactions Between the Epidermal Growth Factor Receptor and Mechanistic Target of Rapamycin Complex 2 in Hepatocellular Carcinoma Cells to Promote Drug Resistance and Xenograft Tumor Progression. Lin XM, et al. Gastroenterology, 2017 Apr. PMID 28065789, Free PMC Article
  3. Overexpression of CHKA contributes to tumor progression and metastasis and predicts poor prognosis in colorectal carcinoma. Hu L, et al. Oncotarget, 2016 Oct 11. PMID 27556502, Free PMC Article
  4. Choline Kinase Alpha (CHKα) as a Therapeutic Target in Pancreatic Ductal Adenocarcinoma: Expression, Predictive Value, and Sensitivity to Inhibitors. Mazarico JM, et al. Mol Cancer Ther, 2016 Feb. PMID 26769123
  5. Choline Kinase Alpha as an Androgen Receptor Chaperone and Prostate Cancer Therapeutic Target. Asim M, et al. J Natl Cancer Inst, 2016 May. PMID 26657335, Free PMC Article

See all (63) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. During proliferation, KDM2B binds to the Box2 located in the Chka promoter repressing its transcription. KDM2B knockdown enhances the levels of CKalpha expression in neuroblast cells and induces neuronal differentiation. These results suggest that KDM2B is required for the appropriate regulation of CKalpha during neuronal differentiation and to the maintaining of the undifferentiated stage of neuroblast cells.
    Title: KDM2B regulates choline kinase expression and neuronal differentiation of neuroblastoma cells.
  2. miR-876-5p was able to significantly decrease the expression level of choline kinase alpha mRNA.
    Title: Downregulation of human choline kinase α gene expression by miR-876-5p.
  3. There is no correlation between single CHKA rs7928739 polymorphism and the incidence of intrauterine fetal death.
    Title: Polymorphic variants of genes involved in choline pathway and the risk of intrauterine fetal death.
  4. This study presents the detail the 2.35 A structure of choline kinase alpha in complex with inhibitor TCD-717.
    Title: Identification of a Unique Inhibitor-Binding Site on Choline Kinase α.
  5. Results indicate that choline kinase alpha (CHKA) contributes to tumor progression and metastasis and may serve as a prognostic biomarker and potential therapeutic target in colorectal carcinoma (CRC).
    Title: Overexpression of CHKA contributes to tumor progression and metastasis and predicts poor prognosis in colorectal carcinoma.
  6. hCKalpha functions as an indispensable regulator that bridges PI4KIIIalpha and hepatitis C virus NS5A and potentiates NS5A-stimulated PI4KIIIalpha activity, which then facilitates the targeting of the ternary complex to the endoplasmic reticulum for viral replication.
    Title: Hepatitis C Virus Subverts Human Choline Kinase-α To Bridge Phosphatidylinositol-4-Kinase IIIα (PI4KIIIα) and NS5A and Upregulates PI4KIIIα Activation, Thereby Promoting the Translocation of the Ternary Complex to the Endoplasmic Reticulum for Viral Replication.
  7. We found levels of CHKA to be increased in human HCCs compared to nontumor tissues, and increased expression to be associated with tumor aggressiveness. Overexpression of CHKA in HCC cell lines increased their invasiveness, resistance to EGFR inhibitors, and ability to form metastatic tumors in mice by promoting interaction of EGFR with mechanistic target of rapamycin complex 2.
    Title: Choline Kinase α Mediates Interactions Between the Epidermal Growth Factor Receptor and Mechanistic Target of Rapamycin Complex 2 in Hepatocellular Carcinoma Cells to Promote Drug Resistance and Xenograft Tumor Progression.
  8. These findings demonstrate that hCKalpha forms a complex with hepatitis C virus NS5A and that hCKalpha activity enhances the targeting of the complex to the endoplasmic reticulum, where hCKalpha protein, not activity, mediates NS5A binding to NS5B, thereby promoting functional membranous viral replication complex assembly and viral RNA replication.
    Title: Human Choline Kinase-α Promotes Hepatitis C Virus RNA Replication through Modulation of Membranous Viral Replication Complex Formation.
  9. Study shows increased CHKA expression in pancreatic ductal adenocarcinoma tumors and may be a predictive marker of response to chemotherapy.
    Title: Choline Kinase Alpha (CHKα) as a Therapeutic Target in Pancreatic Ductal Adenocarcinoma: Expression, Predictive Value, and Sensitivity to Inhibitors.
  10. These data also support the approach of antitumor strategies that destabilize Chk-alpha protein or downregulate PtdCho in breast cancer treatment.
    Title: Choline kinase-α protein and phosphatidylcholine but not phosphocholine are required for breast cancer cell survival.
Submit: New GeneRIF Correction See all GeneRIFs (45)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding IEA
Inferred from Electronic Annotation
more info
  
choline binding IEA
Inferred from Electronic Annotation
more info
  
choline kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
cholinesterase activity IEA
Inferred from Electronic Annotation
more info
  
drug binding IDA
Inferred from Direct Assay
more info
 PubMed 
ethanolamine kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
identical protein binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
CDP-choline pathway IEA
Inferred from Electronic Annotation
more info
  
choline metabolic process IEA
Inferred from Electronic Annotation
more info
  
ethanolamine metabolic process IEA
Inferred from Electronic Annotation
more info
  
lipid metabolic process TAS
Traceable Author Statement
more info
 PubMed 
lipid transport TAS
Traceable Author Statement
more info
 PubMed 
phosphatidylcholine biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
phosphatidylcholine biosynthetic process TAS
Traceable Author Statement
more info
  
phosphatidylethanolamine biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
phosphatidylethanolamine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
phosphatidylethanolamine biosynthetic process TAS
Traceable Author Statement
more info
  
phosphorylation IEA
Inferred from Electronic Annotation
more info
  
response to 3-methylcholanthrene IEA
Inferred from Electronic Annotation
more info
  
response to toxic substance IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
cytosol TAS
Traceable Author Statement
more info
  

General protein information

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Preferred Names
choline kinase alpha
Names
CHETK-alpha
ethanolamine kinase
NP_001268.2
NP_001363148.1
NP_001363149.1
NP_001363150.1
NP_001363151.1
NP_997634.1
XP_016872637.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001277.3 → NP_001268.2  choline kinase alpha isoform a

    See identical proteins and their annotated locations for NP_001268.2

    Status: REVIEWED

    Source sequence(s)
    BC036471, BG703955, BG723115, BI195927, BU617910, D10704
    Consensus CDS
    CCDS8178.1
    UniProtKB/Swiss-Prot
    P35790
    Related
    ENSP00000265689.4, ENST00000265689.9
    Conserved Domains (2) summary
    PLN02236
    Location:99 → 455
    PLN02236; choline kinase
    cd05156
    Location:111 → 452
    ChoK_euk; Euykaryotic Choline Kinase

  2. NM_001376219.1 → NP_001363148.1  choline kinase alpha isoform c

    Status: REVIEWED

    Source sequence(s)
    AP002807, AP002992
    Conserved Domains (1) summary
    cd05156
    Location:111 → 462
    ChoK_euk; Euykaryotic Choline Kinase

  3. NM_001376220.1 → NP_001363149.1  choline kinase alpha isoform d

    Status: REVIEWED

    Source sequence(s)
    AP002807, AP002992
    Conserved Domains (1) summary
    cd05156
    Location:111 → 414
    ChoK_euk; Euykaryotic Choline Kinase

  4. NM_001376221.1 → NP_001363150.1  choline kinase alpha isoform e

    Status: REVIEWED

    Source sequence(s)
    AP002807, AP002992
    Conserved Domains (1) summary
    cd05156
    Location:1 → 330
    ChoK_euk; Euykaryotic Choline Kinase

  5. NM_001376222.1 → NP_001363151.1  choline kinase alpha isoform f

    Status: REVIEWED

    Source sequence(s)
    AP002807, AP002992
    Conserved Domains (1) summary
    cd05156
    Location:1 → 312
    ChoK_euk; Euykaryotic Choline Kinase

  6. NM_212469.2 → NP_997634.1  choline kinase alpha isoform b

    See identical proteins and their annotated locations for NP_997634.1

    Status: REVIEWED

    Source sequence(s)
    AP002807, AP002992
    Consensus CDS
    CCDS8179.1
    UniProtKB/Swiss-Prot
    P35790
    Related
    ENSP00000348454.4, ENST00000356135.9
    Conserved Domains (2) summary
    PLN02236
    Location:99 → 437
    PLN02236; choline kinase
    cd05156
    Location:111 → 434
    ChoK_euk; Euykaryotic Choline Kinase

RNA

  1. NR_164782.1 RNA Sequence

    Status: REVIEWED

    Source sequence(s)
    AP002807, AP002992

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.20200815

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000011.10 Reference GRCh38.p13 Primary Assembly

    Range
    68052859..68121389 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017017148.2 → XP_016872637.1  choline kinase alpha isoform X2

RNA

  1. XR_949773.2 RNA Sequence

  2. XR_949772.2 RNA Sequence

  3. XR_428905.3 RNA Sequence

  4. XR_002957119.1 RNA Sequence

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P35790.3 GenPept UniProtKB/Swiss-Prot:P35790

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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