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Chrnb3 cholinergic receptor, nicotinic, beta polypeptide 3 [ Mus musculus (house mouse) ]

Gene ID: 108043, updated on 3-Jun-2026
Official Symbol
Chrnb3provided by MGI
Official Full Name
cholinergic receptor, nicotinic, beta polypeptide 3provided by MGI
Primary source
MGI:MGI:106212
See related
Ensembl:ENSMUSG00000031492 AllianceGenome:MGI:106212
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Acrb3; 5730417K16Rik
Summary
Predicted to enable acetylcholine binding activity and acetylcholine-gated monoatomic cation-selective channel activity. Predicted to contribute to acetylcholine receptor activity and heterocyclic compound binding activity. Involved in presynaptic modulation of chemical synaptic transmission. Is active in dopaminergic synapse. Is expressed in intestine; liver; nervous system; and retina. Human ortholog(s) of this gene implicated in several diseases, including alcohol dependence; bipolar disorder; cocaine dependence; depressive disorder; and nicotine dependence. Orthologous to human CHRNB3 (cholinergic receptor nicotinic beta 3 subunit). [provided by Alliance of Genome Resources, Jul 2025]
Expression
Low expression observed in reference dataset See more
Orthologs
all
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Try the new Transcripts and proteins table
See Chrnb3 in Genome Data Viewer
Location:
8 A2; 8 16.0 cM
Exon count:
6
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 8 NC_000074.7 (27858688..27889758)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 8 NC_000074.6 (27368711..27399730)

Chromosome 8 - NC_000074.7Genomic Context describing neighboring genes Neighboring gene testis expressed gene 24 Neighboring gene predicted gene, 53409 Neighboring gene cholinergic receptor, nicotinic, alpha polypeptide 6 Neighboring gene predicted gene, 24410

Go to nucleotide: Graphics FASTA GenBank

  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Related articles in PubMed

  1. Decreased anxiety-like behavior in beta3 nicotinic receptor subunit knockout mice. Booker TK, et al. Pharmacol Biochem Behav, 2007 May. PMID 17509676
  2. The β3 subunit of the nicotinic acetylcholine receptor is required for nicotine withdrawal-induced affective but not physical signs or nicotine reward in mice. Jackson AB, et al. Pharmacol Biochem Behav, 2019 Aug. PMID 31145916, Free PMC Article
  3. Roles for N-terminal extracellular domains of nicotinic acetylcholine receptor (nAChR) β3 subunits in enhanced functional expression of mouse α6β2β3- and α6β4β3-nAChRs. Dash B, et al. J Biol Chem, 2014 Oct 10. PMID 25028511, Free PMC Article
  4. Expression of nigrostriatal alpha 6-containing nicotinic acetylcholine receptors is selectively reduced, but not eliminated, by beta 3 subunit gene deletion. Gotti C, et al. Mol Pharmacol, 2005 Jun. PMID 15749993
  5. Menthol Alone Upregulates Midbrain nAChRs, Alters nAChR Subtype Stoichiometry, Alters Dopamine Neuron Firing Frequency, and Prevents Nicotine Reward. Henderson BJ, et al. J Neurosci, 2016 Mar 9. PMID 26961950, Free PMC Article

See all (38) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Beta3 nicotinic subunits do not seem to influence nicotine reward but plays an important role in affective nicotine withdrawal signs.
    Title: The β3 subunit of the nicotinic acetylcholine receptor is required for nicotine withdrawal-induced affective but not physical signs or nicotine reward in mice.
  2. Data provide evidence that the protective genetic variant at rs6474413 identified in human genetic studies reduces gene expression and that decreased beta3 gene expression in mice reduces nicotine intake
    Title: The β3 subunit of the nicotinic acetylcholine receptor: Modulation of gene expression and nicotine consumption.
  3. Chimeric nicotinic acetylcholine receptors reveal the roles for N-terminal extracellular domains of (nAChR) beta3 subunits in the receptor activity and function.
    Title: Roles for N-terminal extracellular domains of nicotinic acetylcholine receptor (nAChR) β3 subunits in enhanced functional expression of mouse α6β2β3- and α6β4β3-nAChRs.
  4. beta3 subunit coexpression promotes function of alpha6*-nAChR
    Title: Modulation of gain-of-function α6*-nicotinic acetylcholine receptor by β3 subunits.
  5. Data show that in Prkce(-/-) mice, conditioned place preference and self-administer behaviors are associated with reduced levels of alpha(6) and beta(3) nicotinic receptor subunit mRNA in the ventral midbrain and striatum.
    Title: Protein kinase C epsilon modulates nicotine consumption and dopamine reward signals in the nucleus accumbens.
  6. beta3-containing nicotinic receptors influence anxiety and may be critical for the continuation of smoking behaviors
    Title: Decreased anxiety-like behavior in beta3 nicotinic receptor subunit knockout mice.
Submit: New GeneRIF Correction

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (3) 
  • Targeted (2)  1 citation

Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables acetylcholine binding IEA
Inferred from Electronic Annotation
more info
  
enables acetylcholine binding ISO
Inferred from Sequence Orthology
more info
  
contributes_to acetylcholine receptor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
contributes_to acetylcholine-gated monoatomic cation-selective channel activity IBA
Inferred from Biological aspect of Ancestor
more info
  
contributes_to acetylcholine-gated monoatomic cation-selective channel activity IEA
Inferred from Electronic Annotation
more info
  
enables acetylcholine-gated monoatomic cation-selective channel activity IEA
Inferred from Electronic Annotation
more info
  
contributes_to acetylcholine-gated monoatomic cation-selective channel activity ISO
Inferred from Sequence Orthology
more info
  
enables extracellular ligand-gated monoatomic ion channel activity IEA
Inferred from Electronic Annotation
more info
  
contributes_to heterocyclic compound binding IEA
Inferred from Electronic Annotation
more info
  
contributes_to heterocyclic compound binding ISO
Inferred from Sequence Orthology
more info
  
enables monoatomic ion channel activity IEA
Inferred from Electronic Annotation
more info
  
enables neurotransmitter receptor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables transmembrane signaling receptor activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in acetylcholine receptor signaling pathway IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in acetylcholine receptor signaling pathway IEA
Inferred from Electronic Annotation
more info
  
involved_in acetylcholine receptor signaling pathway ISO
Inferred from Sequence Orthology
more info
  
involved_in calcium ion transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in chemical synaptic transmission, postsynaptic IEA
Inferred from Electronic Annotation
more info
  
involved_in excitatory postsynaptic potential IEA
Inferred from Electronic Annotation
more info
  
involved_in membrane depolarization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in modulation of chemical synaptic transmission IEA
Inferred from Electronic Annotation
more info
  
involved_in monoatomic ion transmembrane transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in monoatomic ion transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in monoatomic ion transport IEA
Inferred from Electronic Annotation
more info
  
involved_in neuromuscular synaptic transmission IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in presynaptic modulation of chemical synaptic transmission EXP
Inferred from Experiment
more info
 PubMed 
involved_in presynaptic modulation of chemical synaptic transmission IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in presynaptic modulation of chemical synaptic transmission IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in presynaptic modulation of chemical synaptic transmission IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of postsynaptic membrane potential IEA
Inferred from Electronic Annotation
more info
  
involved_in response to nicotine IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in synaptic transmission, cholinergic IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
part_of acetylcholine-gated channel complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of acetylcholine-gated channel complex IEA
Inferred from Electronic Annotation
more info
  
part_of acetylcholine-gated channel complex ISO
Inferred from Sequence Orthology
more info
  
part_of cation channel complex ISO
Inferred from Sequence Orthology
more info
  
is_active_in dopaminergic synapse EXP
Inferred from Experiment
more info
 PubMed 
is_active_in dopaminergic synapse IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in dopaminergic synapse IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in neuron projection IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of neurotransmitter receptor complex ISO
Inferred from Sequence Orthology
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISS
Inferred from Sequence or Structural Similarity
more info
  
located_in postsynaptic membrane IEA
Inferred from Electronic Annotation
more info
  
located_in presynapse IEA
Inferred from Electronic Annotation
more info
  
part_of protein-containing complex IEA
Inferred from Electronic Annotation
more info
  
part_of protein-containing complex ISO
Inferred from Sequence Orthology
more info
  
is_active_in synapse IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in synaptic membrane IEA
Inferred from Electronic Annotation
more info
  
located_in synaptic membrane ISS
Inferred from Sequence or Structural Similarity
more info
  
Preferred Names
neuronal acetylcholine receptor subunit beta-3

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_027454.4 → NP_081730.1  neuronal acetylcholine receptor subunit beta-3 isoform 2 precursor

    See identical proteins and their annotated locations for NP_081730.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) lacks an alternate segment, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1.
    Source sequence(s)
    AK017571, AK030464, BC058193
    Consensus CDS
    CCDS22216.1
    UniProtKB/TrEMBL
    Q3UZS0
    Related
    ENSMUSP00000078428.5, ENSMUST00000079463.12
    Conserved Domains (3) summary
    TIGR00860
    Location:6 → 440
    LIC; Cation transporter family protein
    pfam02931
    Location:35 → 224
    Neur_chan_LBD; Neurotransmitter-gated ion-channel ligand binding domain
    pfam02932
    Location:231 → 437
    Neur_chan_memb; Neurotransmitter-gated ion-channel transmembrane region

  2. NM_173212.4 → NP_775304.1  neuronal acetylcholine receptor subunit beta-3 isoform 1 precursor

    See identical proteins and their annotated locations for NP_775304.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant represents the longer transcript and it encodes the longer protein (isoform 1).
    Source sequence(s)
    AK030464, AK133689, BC058193
    Consensus CDS
    CCDS22217.1
    UniProtKB/Swiss-Prot
    Q8BMN3, Q8R5H3, Q9CYK8
    UniProtKB/TrEMBL
    Q3UZS0
    Related
    ENSMUSP00000052297.4, ENSMUST00000060943.5
    Conserved Domains (3) summary
    TIGR00860
    Location:6 → 455
    LIC; Cation transporter family protein
    pfam02931
    Location:35 → 239
    Neur_chan_LBD; Neurotransmitter-gated ion-channel ligand binding domain
    pfam02932
    Location:246 → 452
    Neur_chan_memb; Neurotransmitter-gated ion-channel transmembrane region

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000074.7 Reference GRCm39 C57BL/6J

    Range
    27858688..27889758
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_030243231.2 → XP_030099091.1  neuronal acetylcholine receptor subunit beta-3 isoform X1

    Conserved Domains (1) summary
    TIGR00860
    Location:1 → 320
    LIC; Cation transporter family protein
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8BMN3.1 GenPept UniProtKB/Swiss-Prot:Q8BMN3

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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