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DDX17 DEAD-box helicase 17 [ Homo sapiens (human) ]

Gene ID: 10521, updated on 3-Mar-2019

Summary

Official Symbol
DDX17provided by HGNC
Official Full Name
DEAD-box helicase 17provided by HGNC
Primary source
HGNC:HGNC:2740
See related
Ensembl:ENSG00000100201 MIM:608469
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
P72; RH70
Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. This protein, and that encoded by DDX5 gene, are more closely related to each other than to any other member of the DEAD box family. This gene can encode multiple isoforms due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) start codon. [provided by RefSeq, Apr 2011]
Expression
Ubiquitous expression in spleen (RPKM 144.6), endometrium (RPKM 136.4) and 25 other tissues See more
Orthologs

Genomic context

See DDX17 in Genome Data Viewer
Location:
22q13.1
Exon count:
13
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) 22 NC_000022.11 (38483438..38506340, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 22 NC_000022.10 (38879443..38903622, complement)

Chromosome 22 - NC_000022.11Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC101927183 Neighboring gene uncharacterized LOC105373029 Neighboring gene potassium voltage-gated channel subfamily J member 4 Neighboring gene KDEL endoplasmic reticulum protein retention receptor 3 Neighboring gene DNA meiotic recombinase 1 Neighboring gene uncharacterized LOC105373031

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
siRNA knockdown of DDX17 decreases intra- and extra-cellular HIV CA(p24) from HeLa cells transfected with env-deleted HIV-1 plasmid, a vesicular stomatitis virus glycoprotein plasmid and specific siRNA. Resulting HIV demonstrates decreased infectivity. PubMed
siRNA knockdown of DDX17 reduced HIV-1 infectivity by 5 times and the extracelluar (supernatant) CA (p24) by a smiliar reduction without affecting the intracellular HIV-1 Gag levels PubMed
Knockdown of DDX17 by siRNA or shRNA inhibits HIV-1 production in HeLa cells and infectivity in TZM-bl cells PubMed
Knockdown of DDX17 by siRNA decreases the levels of total unspliced and spliced mRNAs, and results in reduction of HIV-1 production PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify DEAD (Asp-Glu-Ala-Asp) box helicase 17 (DDX17), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Gag-Pol gag-pol Overexpression of DDX17 DQAD leads to a decrease in frameshift efficiency, suggesting that the helicase activity of DDX17 is involved in maintaining the proper ratio of Gag-Pol expression required for optimal infectivity PubMed
gag-pol Tandem affinity purification and mass spectrometry analysis identify DEAD (Asp-Glu-Ala-Asp) box helicase 17 (DDX17), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Nef nef Tandem affinity purification and mass spectrometry analysis identify DEAD (Asp-Glu-Ala-Asp) box helicase 17 (DDX17), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Pr55(Gag) gag HIV-1 Gag interacts with DDX17 as demonstrated by proximity dependent biotinylation proteomics and validated via immunoprecipitation and western blot analysis PubMed
gag siRNA knockdown of DDX17 DOES NOT affect intracellular Gag levels but does decrease extracellular (supernatant) CA (p24) levels upon HIV-1 infection PubMed
gag Expression of DDX17 DQAD mutant decreases the amount of HIV-1 CA but not Gag, suggesting that the mutant inhibits Gag processing PubMed
gag Tandem affinity purification and mass spectrometry analysis identify DEAD (Asp-Glu-Ala-Asp) box helicase 17 (DDX17), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Rev rev Coexpression of HIV-1 Rev with DDX17 greatly upregulate the expression of HIV-1 CA in HeLa cells PubMed
rev DDX17 interacts with HIV-1 Rev and enhances the Rev function. DDX17 partially co-localizes with Rev in the nucleolus PubMed
rev HIV-1 Rev interacting protein, DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17), is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells. The interaction of Rev with DDX17 is increased by RRE PubMed
Tat tat DEAD (Asp-Glu-Ala-Asp) box helicase 17 (DDX17) is identified to interact with HIV-1 Tat mutant Nullbasic in HeLa cells by LC MS/MS PubMed
capsid gag siRNA knockdown of DDX17 decreases intra- and extra-cellular HIV CA(p24) from HeLa cells transfected with env-deleted HIV-1 plasmid, a vesicular stomatitis virus glycoprotein plasmid and specific siRNA. Resulting HIV demonstrates decreased infectivity. PubMed
gag siRNA knockdown of DDX17 decreases extracellular (supernatant) CA (p24) levels upon HIV-1 infection BUT DOES NOT affect intracellular Gag levels PubMed
gag Coexpression of HIV-1 Rev with DDX17 greatly upregulate the expression of HIV-1 CA in HeLa cells PubMed
gag Expression of DDX17 DQAD mutant decreases the amount of HIV-1 CA but not Gag, suggesting that the mutant inhibits Gag processing PubMed
retropepsin gag-pol Positional proteomics analysis identifies the cleavage of human DEAD (Asp-Glu-Ala-Asp) box helicase 17 (DDX17) at amino acid residues 573-574 by the HIV-1 protease PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

  • RIG-I-like Receptor Signaling, organism-specific biosystem (from WikiPathways)
    RIG-I-like Receptor Signaling, organism-specific biosystemViral pathogen RNA are recognized by host helicases called RIG-I-like receptors (RLRs) that include DDX58 (RIG-I), DHX58 (LGP2), IFIH1 (MDA5), SNW1, and DDX17. These RLR proteins then go on to initia...

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • DKFZp761H2016

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding IEA
Inferred from Electronic Annotation
more info
 
RNA binding HDA PubMed 
RNA helicase activity TAS
Traceable Author Statement
more info
PubMed 
RNA-dependent ATPase activity TAS
Traceable Author Statement
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
transcription coactivator activity IDA
Inferred from Direct Assay
more info
PubMed 
Process Evidence Code Pubs
RNA processing TAS
Traceable Author Statement
more info
PubMed 
alternative mRNA splicing, via spliceosome IMP
Inferred from Mutant Phenotype
more info
PubMed 
androgen receptor signaling pathway IMP
Inferred from Mutant Phenotype
more info
PubMed 
defense response to virus IEA
Inferred from Electronic Annotation
more info
 
epithelial to mesenchymal transition IMP
Inferred from Mutant Phenotype
more info
PubMed 
gene silencing by RNA IEA
Inferred from Electronic Annotation
more info
 
intracellular estrogen receptor signaling pathway IMP
Inferred from Mutant Phenotype
more info
PubMed 
miRNA metabolic process IMP
Inferred from Mutant Phenotype
more info
PubMed 
myoblast differentiation ISS
Inferred from Sequence or Structural Similarity
more info
 
positive regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
PubMed 
rRNA processing IEA
Inferred from Electronic Annotation
more info
 
regulation of alternative mRNA splicing, via spliceosome IMP
Inferred from Mutant Phenotype
more info
PubMed 
regulation of skeletal muscle cell differentiation IMP
Inferred from Mutant Phenotype
more info
PubMed 
regulation of transcription by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
PubMed 
Component Evidence Code Pubs
cytosol IEA
Inferred from Electronic Annotation
more info
 
membrane HDA PubMed 
nuclear speck IDA
Inferred from Direct Assay
more info
 
nucleolus IEA
Inferred from Electronic Annotation
more info
 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
probable ATP-dependent RNA helicase DDX17
Names
DEAD (Asp-Glu-Ala-Asp) box helicase 17
DEAD (Asp-Glu-Ala-Asp) box polypeptide 17
DEAD box protein p72
DEAD box protein p82
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 17 (72kD)
RNA-dependent helicase p72
NP_001091974.1
NP_006377.2

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_029642.1 RefSeqGene

    Range
    5001..27903
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001098504.1NP_001091974.1  probable ATP-dependent RNA helicase DDX17 isoform 3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) uses a different donor splice site (6 nt downstream) in the penultimate coding exon, but initiates translation from the same non-AUG (CUG) codon as transcript variant 1, resulting in an isoform (3) that is 2 aa longer than isoform 1. Alternate translation from an in-frame, downstream AUG yields a shorter isoform.
    Source sequence(s)
    AB209595, AK024985, AL080113, BP353248, CB215934, Z97056
    Consensus CDS
    CCDS46706.1
    UniProtKB/TrEMBL
    A0A1W2PQ51, Q59F66
    Related
    ENSP00000380033.3, ENST00000396821.7
    Conserved Domains (3) summary
    smart00487
    Location:191393
    DEXDc; DEAD-like helicases superfamily
    cd00079
    Location:389521
    HELICc; Helicase superfamily c-terminal domain; associated with DEXDc-, DEAD-, and DEAH-box proteins, yeast initiation factor 4A, Ski2p, and Hepatitis C virus NS3 helicases; this domain is found in a wide variety of helicases and helicase related proteins; may ...
    cd00268
    Location:173378
    DEADc; DEAD-box helicases. A diverse family of proteins involved in ATP-dependent RNA unwinding, needed in a variety of cellular processes including splicing, ribosome biogenesis and RNA degradation. The name derives from the sequence of the Walker B motif ...
  2. NM_006386.5NP_006377.2  probable ATP-dependent RNA helicase DDX17 isoform 1

    See identical proteins and their annotated locations for NP_006377.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes isoform 1 (also known as p82) through the use of a non-AUG (CUG) translation initiation codon. Alternate translation from an in-frame, downstream AUG results in a shorter isoform (known as p72).
    Source sequence(s)
    AK024985, AL080113, BP353248, CB215934, Z97056
    Consensus CDS
    CCDS33646.1
    UniProtKB/Swiss-Prot
    Q92841
    Related
    ENSP00000385536.1, ENST00000403230.1
    Conserved Domains (3) summary
    smart00487
    Location:191393
    DEXDc; DEAD-like helicases superfamily
    cd00079
    Location:389521
    HELICc; Helicase superfamily c-terminal domain; associated with DEXDc-, DEAD-, and DEAH-box proteins, yeast initiation factor 4A, Ski2p, and Hepatitis C virus NS3 helicases; this domain is found in a wide variety of helicases and helicase related proteins; may ...
    cd00268
    Location:173378
    DEADc; DEAD-box helicases. A diverse family of proteins involved in ATP-dependent RNA unwinding, needed in a variety of cellular processes including splicing, ribosome biogenesis and RNA degradation. The name derives from the sequence of the Walker B motif ...

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000022.11 Reference GRCh38.p12 Primary Assembly

    Range
    38483438..38506340 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_001098505.1: Suppressed sequence

    Description
    NM_001098505.1: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript and the protein.
  2. NM_030881.3: Suppressed sequence

    Description
    NM_030881.3: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript and the protein.
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