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IFITM3 interferon induced transmembrane protein 3 [ Homo sapiens (human) ]

Gene ID: 10410, updated on 17-Jun-2019

Summary

Official Symbol
IFITM3provided by HGNC
Official Full Name
interferon induced transmembrane protein 3provided by HGNC
Primary source
HGNC:HGNC:5414
See related
Ensembl:ENSG00000142089 MIM:605579
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
1-8U; IP15; DSPA2b
Summary
The protein encoded by this gene is an interferon-induced membrane protein that helps confer immunity to influenza A H1N1 virus, West Nile virus, and dengue virus. Two transcript variants, only one of them protein-coding, have been found for this gene. Another variant encoding an N-terminally truncated isoform has been reported, but the full-length nature of this variant has not been determined. [provided by RefSeq, May 2012]
Expression
Ubiquitous expression in liver (RPKM 370.9), placenta (RPKM 358.2) and 25 other tissues See more
Orthologs

Genomic context

See IFITM3 in Genome Data Viewer
Location:
11p15.5
Exon count:
2
Annotation release Status Assembly Chr Location
109.20190607 current GRCh38.p13 (GCF_000001405.39) 11 NC_000011.10 (319676..320860, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 11 NC_000011.9 (319673..320914, complement)

Chromosome 11 - NC_000011.10Genomic Context describing neighboring genes Neighboring gene interferon induced transmembrane protein 2 Neighboring gene interferon induced transmembrane protein 1 Neighboring gene uncharacterized LOC105376504 Neighboring gene uncharacterized LOC105376505

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
HIV-1 incorporates IFITM3 into virions but this incorporation does not strictly correlate with the phenotype of IFITM inhibition of HIV-1 cell-to-cell infection PubMed
HIV-1 CXCR4-tropic virus replication is restricted by IFITM2 and IFITM3 as shown through shRNA knockdown PubMed
HIV-1 infectivity is inhibited when IFITM3 is expressed by the virus producing cell (293T cells) PubMed
HIV-1 cell-to-cell transmission (Jurkat-to-Jurkat, 293T-to-Jurkat, PM1 to Jurkat, PBMCs to Jurkat) is inhibited by IFITM3 and this inhibition appears to be regulated by the IFITM protein C terminus, is IFITM-species specific and virus-strain specific PubMed
Knockdown of IFITM1/2/3 by siRNA enhances virus transmission to target cells and the production of virions in CD4+ T cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 gp120 shedding is induced by IFITM3 PubMed
env HIV-1 gp120 interacts with IFITM3 PubMed
env HIV-1 Env processing from gp160 to gp120/41 is inhibited by IFITM3 PubMed
env The exposure to HIV-1 gp120 upregulates the IFITM3 level in HeLa cells PubMed
env Tyrosine kinase Fyn-mediated phosphorylation of IFITM3 at position Y20 induces localization of IFITM3 at the endocytic compartments, which impairs inhibition of Env-mediated entry by IFITM3 delta(1-21) mutant PubMed
env The expression of IFITM3 delta(1-21) mutant inhibits HIV-1 gp120/gp41 Env-mediated entry into human SupT1 cells, suggesting that the first 21 amino acids in IFITM3 are not required for inhibition of Env-mediated entry PubMed
Envelope surface glycoprotein gp160, precursor env HIV-1 gp160 interacts with IFITM3 PubMed
env IFITM2/3 proteins are co-localize with HIV-1 Env in the cell interior, while surface-associated IFITM2/3 proteins are present at virus budding sites containing HIV-1 Gag and Env proteins in CD4+ T cells PubMed
Envelope transmembrane glycoprotein gp41 env HIV-1 Env processing from gp160 to gp120/41 is inhibited by IFITM3 PubMed
env HIV-1 gp41 interacts with IFITM3 PubMed
env Tyrosine kinase Fyn-mediated phosphorylation of IFITM3 at position Y20 induces localization of IFITM3 at the endocytic compartments, which impairs inhibition of Env-mediated entry by IFITM3 delta(1-21) mutant PubMed
env The expression of IFITM3 delta(1-21) mutant inhibits HIV-1 gp120/gp41 Env-mediated entry into human SupT1 cells, suggesting that the first 21 amino acids in IFITM3 are not required for inhibition of Env-mediated entry PubMed
Nef nef The small molecule PKR inhibitor C16 inhibits the IFITM3-induced suppression of HIV-1 Gag, Vif, and Nef protein expression PubMed
nef IFITM3 significantly downregulates the expression of HIV-1 Nef by interfering with Tat/TAR-mediated expression PubMed
Pr55(Gag) gag IFITM1, IFITM2, and IFITM3 proteins (IFITMs) co-localize with HIV-1 Gag in virus-producing cells and co-assemble with Gag into budding particles PubMed
gag Endogenous IFITM3 inhibits virus infectivity by measuement of Gag+ target cells PubMed
gag Endogenous IFITM1/2/3 proteins are upregulated in activated CD4+ T cells and incorporated into HIV-1 Gag/p24-associated virions during infection PubMed
gag IFITM2/3 proteins are co-localize with HIV-1 Env in the cell interior, while surface-associated IFITM2/3 proteins are present at virus budding sites containing HIV-1 Gag and Env proteins in CD4+ T cells PubMed
gag The small molecule PKR inhibitor C16 inhibits the IFITM3-induced suppression of HIV-1 Gag, Vif, and Nef protein expression PubMed
gag IFITM3 significantly downregulates the expression of HIV-1 Gag and CA by interfering with both Rev/RRE- and Tat/TAR-mediated expression PubMed
Vif vif The small molecule PKR inhibitor C16 inhibits the IFITM3-induced suppression of HIV-1 Gag, Vif, and Nef protein expression PubMed
vif IFITM3 significantly downregulates the expression of HIV-1 Vif by interfering with both Rev/RRE- and Tat/TAR-mediated expression PubMed
Vpr vpr HIV-1 Vpr upregulates the gene expression of IFITM3 in human monocyte-derived dendritic cells PubMed
vpr IFITM1, 2 or 3 marginally inhibit the entry of HIV-1 NL4-3 and SIV Mac into TZM-bl by measuring the cleavage of CCF2 by BlaM-Vpr that enters the cytoplasm together with viral cores PubMed
capsid gag IFITM1, IFITM2, and IFITM3 proteins (IFITMs) co-localize with HIV-1 CA in velocity gradients, indicating that IFITMs are virion-associated proteins PubMed
gag Endogenous IFITM1/2/3 proteins are upregulated in activated CD4+ T cells and incorporated into HIV-1 Gag/p24-associated virions during infection PubMed
gag IFITM3 significantly downregulates the expression of HIV-1 Gag and CA by interfering with both Rev/RRE- and Tat/TAR-mediated expression PubMed
matrix gag IFITM3 associates with HIV-1 viral membrane-associated MA rather than with the core in virions PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

  • Cytokine Signaling in Immune system, organism-specific biosystem (from REACTOME)
    Cytokine Signaling in Immune system, organism-specific biosystemCytokines are small proteins that regulate and mediate immunity, inflammation, and hematopoiesis. They are secreted in response to immune stimuli, and usually act briefly, locally, at very low concen...
  • Immune System, organism-specific biosystem (from REACTOME)
    Immune System, organism-specific biosystemHumans are exposed to millions of potential pathogens daily, through contact, ingestion, and inhalation. Our ability to avoid infection depends on the adaptive immune system and during the first crit...
  • Interferon Signaling, organism-specific biosystem (from REACTOME)
    Interferon Signaling, organism-specific biosystemInterferons (IFNs) are cytokines that play a central role in initiating immune responses, especially antiviral and antitumor effects. There are three types of IFNs:Type I (IFN-alpha, -beta and others...
  • Interferon alpha/beta signaling, organism-specific biosystem (from REACTOME)
    Interferon alpha/beta signaling, organism-specific biosystemType I interferons (IFNs) are composed of various genes including IFN alpha (IFNA), beta (IFNB), omega, epsilon, and kappa. In humans the IFNA genes are composed of more than 13 subfamily genes, wher...

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
Process Evidence Code Pubs
defense response to virus IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
defense response to virus IDA
Inferred from Direct Assay
more info
PubMed 
immune response TAS
Traceable Author Statement
more info
PubMed 
negative regulation of viral entry into host cell IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
negative regulation of viral entry into host cell IDA
Inferred from Direct Assay
more info
PubMed 
negative regulation of viral entry into host cell IMP
Inferred from Mutant Phenotype
more info
PubMed 
negative regulation of viral genome replication IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
negative regulation of viral genome replication IDA
Inferred from Direct Assay
more info
PubMed 
negative regulation of viral transcription IDA
Inferred from Direct Assay
more info
PubMed 
response to interferon-alpha IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
response to interferon-alpha IDA
Inferred from Direct Assay
more info
PubMed 
response to interferon-beta IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
response to interferon-beta IDA
Inferred from Direct Assay
more info
PubMed 
response to interferon-gamma IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
response to interferon-gamma IDA
Inferred from Direct Assay
more info
PubMed 
response to virus IDA
Inferred from Direct Assay
more info
PubMed 
type I interferon signaling pathway IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
type I interferon signaling pathway TAS
Traceable Author Statement
more info
 
Component Evidence Code Pubs
integral component of membrane IEA
Inferred from Electronic Annotation
more info
 
late endosome membrane IEA
Inferred from Electronic Annotation
more info
 
lysosomal membrane IEA
Inferred from Electronic Annotation
more info
 
plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
plasma membrane IDA
Inferred from Direct Assay
more info
PubMed 
plasma membrane TAS
Traceable Author Statement
more info
 
protein-containing complex IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
interferon-induced transmembrane protein 3
Names
dispanin subfamily A member 2b
interferon-inducible protein 1-8U

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_032755.1 RefSeqGene

    Range
    5001..6242
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_021034.3NP_066362.2  interferon-induced transmembrane protein 3

    See identical proteins and their annotated locations for NP_066362.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longer, protein-coding transcript.
    Source sequence(s)
    AI355192, DB261103
    Consensus CDS
    CCDS41585.1
    UniProtKB/Swiss-Prot
    Q01628
    Related
    ENSP00000382707.4, ENST00000399808.5
    Conserved Domains (1) summary
    pfam04505
    Location:55121
    CD225; Interferon-induced transmembrane protein

RNA

  1. NR_049759.2 RNA Sequence

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) uses an alternate splice junction at the 5' end of a coding exon compared to variant 1, that causes a frameshift. The resulting transcript is not thought to be protein-coding.
    Source sequence(s)
    BQ937265, DB261103

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000011.10 Reference GRCh38.p13 Primary Assembly

    Range
    319676..320860 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
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