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PAK4 p21 (RAC1) activated kinase 4 [ Homo sapiens (human) ]

Gene ID: 10298, updated on 4-Feb-2024

Summary

Official Symbol
PAK4provided by HGNC
Official Full Name
p21 (RAC1) activated kinase 4provided by HGNC
Primary source
HGNC:HGNC:16059
See related
Ensembl:ENSG00000130669 MIM:605451; AllianceGenome:HGNC:16059
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Summary
PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in prostate (RPKM 14.9), kidney (RPKM 12.6) and 25 other tissues See more
Orthologs
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Genomic context

See PAK4 in Genome Data Viewer
Location:
19q13.2
Exon count:
14
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 19 NC_000019.10 (39125786..39182816)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 19 NC_060943.1 (41929765..41986971)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 19 NC_000019.9 (39616426..39673456)

Chromosome 19 - NC_000019.10Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC124904801 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14611 Neighboring gene NANOG hESC enhancer GRCh37_chr19:39549145-39549646 Neighboring gene BRD4-independent group 4 enhancer GRCh37_chr19:39564087-39565286 Neighboring gene Sharpr-MPRA regulatory region 11111 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:39574861-39575361 Neighboring gene acid phosphatase 7, tartrate resistant (putative) Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14612 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:39622545-39623126 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:39623127-39623707 Neighboring gene uncharacterized LOC124904714 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:39625781-39626526 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:39629677-39630266 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:39634730-39635726 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:39635727-39636721 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:39641079-39641580 Neighboring gene uncharacterized LOC124904716 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:39647059-39647724 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:39648391-39649056 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:39649057-39649720 Neighboring gene NCCRP1, F-box associated domain containing Neighboring gene syncollin

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
Knockdown of p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4) by shRNA library screening inhibits HIV-1 replication in cultured Jurkat T-cells PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
 
enables cadherin binding involved in cell-cell adhesion HDA PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables protein kinase activity NAS
Non-traceable Author Statement
more info
PubMed 
enables protein serine kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables protein serine/threonine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
 
enables protein serine/threonine kinase activity IDA
Inferred from Direct Assay
more info
PubMed 
enables protein serine/threonine kinase activity IMP
Inferred from Mutant Phenotype
more info
PubMed 
Process Evidence Code Pubs
involved_in apoptotic process TAS
Traceable Author Statement
more info
PubMed 
involved_in cell cycle IEA
Inferred from Electronic Annotation
more info
 
involved_in cell migration TAS
Traceable Author Statement
more info
PubMed 
involved_in cell-cell adhesion IEA
Inferred from Electronic Annotation
more info
 
involved_in cellular response to organic cyclic compound IEA
Inferred from Electronic Annotation
more info
 
involved_in cytoskeleton organization TAS
Traceable Author Statement
more info
PubMed 
involved_in dendritic spine development IEA
Inferred from Electronic Annotation
more info
 
involved_in intracellular signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in negative regulation of endothelial cell apoptotic process IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in phosphorylation IEA
Inferred from Electronic Annotation
more info
 
involved_in positive regulation of angiogenesis IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in protein phosphorylation IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in regulation of MAPK cascade IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in regulation of cell growth TAS
Traceable Author Statement
more info
PubMed 
involved_in signal transduction TAS
Traceable Author Statement
more info
PubMed 
Component Evidence Code Pubs
located_in Golgi apparatus TAS
Traceable Author Statement
more info
PubMed 
located_in adherens junction HDA PubMed 
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
 
located_in cytosol TAS
Traceable Author Statement
more info
 
located_in focal adhesion HDA PubMed 

General protein information

Preferred Names
serine/threonine-protein kinase PAK 4
Names
p21 protein (Cdc42/Rac)-activated kinase 4
p21(CDKN1A)-activated kinase 4
protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
NP_001014831.1
NP_001014832.1
NP_001014834.1
NP_001014835.1
NP_001381430.1
NP_005875.1
XP_011524618.1
XP_011524619.1
XP_011524622.1
XP_024307082.1
XP_047293990.1
XP_047293991.1
XP_047293992.1
XP_047293993.1
XP_047293994.1
XP_047293996.1
XP_047293997.1
XP_054175478.1
XP_054175479.1
XP_054175480.1
XP_054175481.1
XP_054175482.1
XP_054175483.1
XP_054175484.1
XP_054175485.1
XP_054175486.1
XP_054175487.1
XP_054175488.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_047118.2 RefSeqGene

    Range
    5002..58620
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001014831.3NP_001014831.1  serine/threonine-protein kinase PAK 4 isoform 1

    See identical proteins and their annotated locations for NP_001014831.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) has an additional segment in the 5' UTR, and encodes the same isoform (1), as compared to variant 1.
    Source sequence(s)
    AB032968, AC011443, BC025282, BG773480
    Consensus CDS
    CCDS12528.1
    UniProtKB/Swiss-Prot
    B4DGG6, O96013, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
    UniProtKB/TrEMBL
    A8K724
    Related
    ENSP00000469413.1, ENST00000593690.5
    Conserved Domains (2) summary
    cd06657
    Location:300591
    STKc_PAK4; Catalytic domain of the Serine/Threonine Kinase, p21-activated kinase 4
    cd01093
    Location:1055
    CRIB_PAK_like; PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the ...
  2. NM_001014832.2NP_001014832.1  serine/threonine-protein kinase PAK 4 isoform 1

    See identical proteins and their annotated locations for NP_001014832.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) lacks an exon in the 5' UTR, and encodes the same isoform (1), as compared to variant 1.
    Source sequence(s)
    AB032968, AC011443, BC011368, BC025282, BG773480
    Consensus CDS
    CCDS12528.1
    UniProtKB/Swiss-Prot
    B4DGG6, O96013, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
    UniProtKB/TrEMBL
    A8K724
    Related
    ENSP00000351049.2, ENST00000358301.7
    Conserved Domains (2) summary
    cd06657
    Location:300591
    STKc_PAK4; Catalytic domain of the Serine/Threonine Kinase, p21-activated kinase 4
    cd01093
    Location:1055
    CRIB_PAK_like; PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the ...
  3. NM_001014834.3NP_001014834.1  serine/threonine-protein kinase PAK 4 isoform 2

    See identical proteins and their annotated locations for NP_001014834.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (5) lacks an exon in the 5' UTR and an in-frame exon in the coding region, as compared to variant 1. The encoded isoform (2) thus lacks an internal segment, as compared to isoform 1.
    Source sequence(s)
    AB032968, AC011443, AL834236, BG773480
    Consensus CDS
    CCDS33019.1
    UniProtKB/TrEMBL
    B4DE88
    Related
    ENSP00000470284.1, ENST00000599470.5
    Conserved Domains (2) summary
    cd01093
    Location:1055
    CRIB_PAK_like; PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the ...
    cl21453
    Location:147438
    PKc_like; Protein Kinases, catalytic domain
  4. NM_001014835.2NP_001014835.1  serine/threonine-protein kinase PAK 4 isoform 2

    See identical proteins and their annotated locations for NP_001014835.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) lacks an in-frame exon in the coding region, as compared to variant 1. The encoded isoform (2) thus lacks an internal segment, as compared to isoform 1.
    Source sequence(s)
    AB032968, AC011443, BG773480
    Consensus CDS
    CCDS33019.1
    UniProtKB/TrEMBL
    B4DE88
    Related
    ENSP00000471157.1, ENST00000599386.5
    Conserved Domains (2) summary
    cd01093
    Location:1055
    CRIB_PAK_like; PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the ...
    cl21453
    Location:147438
    PKc_like; Protein Kinases, catalytic domain
  5. NM_001394501.1NP_001381430.1  serine/threonine-protein kinase PAK 4 isoform 1

    Status: REVIEWED

    Source sequence(s)
    AC011443
    Consensus CDS
    CCDS12528.1
    UniProtKB/Swiss-Prot
    B4DGG6, O96013, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
    UniProtKB/TrEMBL
    A8K724
    Conserved Domains (2) summary
    cd06657
    Location:300591
    STKc_PAK4; Catalytic domain of the Serine/Threonine Kinase, p21-activated kinase 4
    cd01093
    Location:1055
    CRIB_PAK_like; PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the ...
  6. NM_005884.5NP_005875.1  serine/threonine-protein kinase PAK 4 isoform 1

    See identical proteins and their annotated locations for NP_005875.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (1).
    Source sequence(s)
    AB032968, AF005046, BG773480
    Consensus CDS
    CCDS12528.1
    UniProtKB/Swiss-Prot
    B4DGG6, O96013, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
    UniProtKB/TrEMBL
    A8K724
    Related
    ENSP00000353625.3, ENST00000360442.8
    Conserved Domains (2) summary
    cd06657
    Location:300591
    STKc_PAK4; Catalytic domain of the Serine/Threonine Kinase, p21-activated kinase 4
    cd01093
    Location:1055
    CRIB_PAK_like; PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the ...

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000019.10 Reference GRCh38.p14 Primary Assembly

    Range
    39125786..39182816
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011526316.2XP_011524618.1  serine/threonine-protein kinase PAK 4 isoform X1

    See identical proteins and their annotated locations for XP_011524618.1

    UniProtKB/Swiss-Prot
    B4DGG6, O96013, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
    UniProtKB/TrEMBL
    A8K724
    Conserved Domains (2) summary
    cd06657
    Location:300591
    STKc_PAK4; Catalytic domain of the Serine/Threonine Kinase, p21-activated kinase 4
    cd01093
    Location:1055
    CRIB_PAK_like; PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the ...
  2. XM_011526320.2XP_011524622.1  serine/threonine-protein kinase PAK 4 isoform X2

    See identical proteins and their annotated locations for XP_011524622.1

    UniProtKB/TrEMBL
    B4DE88
    Conserved Domains (2) summary
    cd01093
    Location:1055
    CRIB_PAK_like; PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the ...
    cl21453
    Location:147438
    PKc_like; Protein Kinases, catalytic domain
  3. XM_047438034.1XP_047293990.1  serine/threonine-protein kinase PAK 4 isoform X1

    UniProtKB/Swiss-Prot
    B4DGG6, O96013, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
    UniProtKB/TrEMBL
    A8K724
  4. XM_024451314.2XP_024307082.1  serine/threonine-protein kinase PAK 4 isoform X2

    UniProtKB/TrEMBL
    B4DE88
    Conserved Domains (2) summary
    cd01093
    Location:1055
    CRIB_PAK_like; PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the ...
    cl21453
    Location:147438
    PKc_like; Protein Kinases, catalytic domain
  5. XM_011526317.3XP_011524619.1  serine/threonine-protein kinase PAK 4 isoform X1

    See identical proteins and their annotated locations for XP_011524619.1

    UniProtKB/Swiss-Prot
    B4DGG6, O96013, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
    UniProtKB/TrEMBL
    A8K724
    Conserved Domains (2) summary
    cd06657
    Location:300591
    STKc_PAK4; Catalytic domain of the Serine/Threonine Kinase, p21-activated kinase 4
    cd01093
    Location:1055
    CRIB_PAK_like; PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the ...
  6. XM_047438040.1XP_047293996.1  serine/threonine-protein kinase PAK 4 isoform X2

    UniProtKB/TrEMBL
    B4DE88
  7. XM_047438037.1XP_047293993.1  serine/threonine-protein kinase PAK 4 isoform X1

    UniProtKB/Swiss-Prot
    B4DGG6, O96013, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
    UniProtKB/TrEMBL
    A8K724
  8. XM_047438038.1XP_047293994.1  serine/threonine-protein kinase PAK 4 isoform X1

    UniProtKB/Swiss-Prot
    B4DGG6, O96013, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
    UniProtKB/TrEMBL
    A8K724
  9. XM_047438036.1XP_047293992.1  serine/threonine-protein kinase PAK 4 isoform X1

    UniProtKB/Swiss-Prot
    B4DGG6, O96013, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
    UniProtKB/TrEMBL
    A8K724
  10. XM_047438035.1XP_047293991.1  serine/threonine-protein kinase PAK 4 isoform X1

    UniProtKB/Swiss-Prot
    B4DGG6, O96013, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
    UniProtKB/TrEMBL
    A8K724
  11. XM_047438041.1XP_047293997.1  serine/threonine-protein kinase PAK 4 isoform X2

    UniProtKB/TrEMBL
    B4DE88

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060943.1 Alternate T2T-CHM13v2.0

    Range
    41929765..41986971
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054319503.1XP_054175478.1  serine/threonine-protein kinase PAK 4 isoform X1

    UniProtKB/Swiss-Prot
    B4DGG6, O96013, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
    UniProtKB/TrEMBL
    A8K724
  2. XM_054319510.1XP_054175485.1  serine/threonine-protein kinase PAK 4 isoform X2

    UniProtKB/TrEMBL
    B4DE88
  3. XM_054319504.1XP_054175479.1  serine/threonine-protein kinase PAK 4 isoform X1

    UniProtKB/Swiss-Prot
    B4DGG6, O96013, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
    UniProtKB/TrEMBL
    A8K724
  4. XM_054319505.1XP_054175480.1  serine/threonine-protein kinase PAK 4 isoform X1

    UniProtKB/Swiss-Prot
    B4DGG6, O96013, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
    UniProtKB/TrEMBL
    A8K724
  5. XM_054319512.1XP_054175487.1  serine/threonine-protein kinase PAK 4 isoform X2

    UniProtKB/TrEMBL
    B4DE88
  6. XM_054319511.1XP_054175486.1  serine/threonine-protein kinase PAK 4 isoform X2

    UniProtKB/TrEMBL
    B4DE88
  7. XM_054319508.1XP_054175483.1  serine/threonine-protein kinase PAK 4 isoform X1

    UniProtKB/Swiss-Prot
    B4DGG6, O96013, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
    UniProtKB/TrEMBL
    A8K724
  8. XM_054319509.1XP_054175484.1  serine/threonine-protein kinase PAK 4 isoform X1

    UniProtKB/Swiss-Prot
    B4DGG6, O96013, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
    UniProtKB/TrEMBL
    A8K724
  9. XM_054319507.1XP_054175482.1  serine/threonine-protein kinase PAK 4 isoform X1

    UniProtKB/Swiss-Prot
    B4DGG6, O96013, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
    UniProtKB/TrEMBL
    A8K724
  10. XM_054319506.1XP_054175481.1  serine/threonine-protein kinase PAK 4 isoform X1

    UniProtKB/Swiss-Prot
    B4DGG6, O96013, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
    UniProtKB/TrEMBL
    A8K724
  11. XM_054319513.1XP_054175488.1  serine/threonine-protein kinase PAK 4 isoform X2

    UniProtKB/TrEMBL
    B4DE88

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_001014833.1: Suppressed sequence

    Description
    NM_001014833.1: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript and the protein.