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ACOT8 acyl-CoA thioesterase 8 [ Homo sapiens (human) ]

Gene ID: 10005, updated on 21-Dec-2019

Summary

Official Symbol
ACOT8provided by HGNC
Official Full Name
acyl-CoA thioesterase 8provided by HGNC
Primary source
HGNC:HGNC:15919
See related
Ensembl:ENSG00000101473 MIM:608123
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
hTE; NAP1; PTE1; PTE2; PTE-1; PTE-2; HNAACTE; hACTE-III
Summary
The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010]
Expression
Ubiquitous expression in adrenal (RPKM 10.6), brain (RPKM 10.3) and 25 other tissues See more
Orthologs

Genomic context

See ACOT8 in Genome Data Viewer
Location:
20q13.12
Exon count:
7
Annotation release Status Assembly Chr Location
109.20191205 current GRCh38.p13 (GCF_000001405.39) 20 NC_000020.11 (45841721..45857405, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 20 NC_000020.10 (44470360..44486048, complement)

Chromosome 20 - NC_000020.11Genomic Context describing neighboring genes Neighboring gene ubiquitin conjugating enzyme E2 C Neighboring gene troponin C2, fast skeletal type Neighboring gene sorting nexin family member 21 Neighboring gene zinc finger SWIM-type containing 3 Neighboring gene zinc finger SWIM-type containing 1

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Nef nef HIV-1 Nef (from strains NA7 and NL4-3 only) directly interacts with ACOT8 in 293T cells and this interaction is mediated by residues in the Nef core domain as shown by FACS-Forster resonance energy transfer (FRET)-based methods PubMed
nef HIV-1 Nef is identified to have a physical interaction with acyl-CoA thioesterase 8 (ACOT8) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
nef Point mutations in HIV-1 Nef at amino acids Asp108, Leu121, Pro122, and Asp123 abrogate the ability of Nef to bind hTE , downregulate CD4 and MHC class I, and dimerize, indicating a key role for this region of Nef in mediating most of its known functions PubMed
nef Amino acids 108-152 mediate binding of HIV-1 Nef to the human Thioesterase II protein (hTE/ACOT8), an interaction that enhances hTE enzymatic activity PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
CoA hydrolase activity TAS
Traceable Author Statement
more info
 
acetoacetyl-CoA hydrolase activity IEA
Inferred from Electronic Annotation
more info
 
acetyl-CoA hydrolase activity TAS
Traceable Author Statement
more info
 
acyl-CoA hydrolase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
acyl-CoA hydrolase activity IDA
Inferred from Direct Assay
more info
PubMed 
carboxylic ester hydrolase activity IEA
Inferred from Electronic Annotation
more info
 
choloyl-CoA hydrolase activity IEA
Inferred from Electronic Annotation
more info
 
hydroxymethylglutaryl-CoA hydrolase activity IEA
Inferred from Electronic Annotation
more info
 
NOT long-chain acyl-CoA hydrolase activity IDA
Inferred from Direct Assay
more info
PubMed 
medium-chain acyl-CoA hydrolase activity IDA
Inferred from Direct Assay
more info
PubMed 
myristoyl-CoA hydrolase activity IEA
Inferred from Electronic Annotation
more info
 
palmitoyl-CoA hydrolase activity IDA
Inferred from Direct Assay
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
succinyl-CoA hydrolase activity IEA
Inferred from Electronic Annotation
more info
 
Process Evidence Code Pubs
acyl-CoA metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
acyl-CoA metabolic process IDA
Inferred from Direct Assay
more info
PubMed 
acyl-CoA metabolic process TAS
Traceable Author Statement
more info
 
alpha-linolenic acid metabolic process TAS
Traceable Author Statement
more info
 
bile acid biosynthetic process TAS
Traceable Author Statement
more info
 
dicarboxylic acid catabolic process IDA
Inferred from Direct Assay
more info
PubMed 
fatty acid beta-oxidation using acyl-CoA oxidase TAS
Traceable Author Statement
more info
 
fatty acid catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
negative regulation of CD4 biosynthetic process IDA
Inferred from Direct Assay
more info
PubMed 
peroxisome fission IDA
Inferred from Direct Assay
more info
PubMed 
protein targeting to peroxisome TAS
Traceable Author Statement
more info
 
viral process IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
cytosol TAS
Traceable Author Statement
more info
 
peroxisomal matrix IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
peroxisomal matrix IDA
Inferred from Direct Assay
more info
PubMed 
peroxisomal matrix IMP
Inferred from Mutant Phenotype
more info
PubMed 
peroxisomal matrix TAS
Traceable Author Statement
more info
 

General protein information

Preferred Names
acyl-coenzyme A thioesterase 8
Names
HIV-Nef associated acyl-CoA thioesterase
Nef (lentivirus myristoylated factor) associated protein 1
choloyl-CoA hydrolase
choloyl-coenzyme A thioesterase
long-chain fatty-acyl-CoA hydrolase
palmitoyl-CoA hydrolase
peroxisomal acyl-CoA thioesterase 1
peroxisomal acyl-CoA thioesterase 2
peroxisomal acyl-coenzyme A thioester hydrolase 1
peroxisomal long-chain acyl-CoA thioesterase 1
thioesterase II
thioesterase III
NP_005460.2
XP_005260296.1
XP_011526782.1

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_005469.4NP_005460.2  acyl-coenzyme A thioesterase 8

    See identical proteins and their annotated locations for NP_005460.2

    Status: REVIEWED

    Source sequence(s)
    BC117157, BM986979, DB470803, X86032
    Consensus CDS
    CCDS13378.1
    UniProtKB/Swiss-Prot
    O14734
    Related
    ENSP00000217455.4, ENST00000217455.9
    Conserved Domains (1) summary
    TIGR00189
    Location:35309
    tesB; acyl-CoA thioesterase II

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.20191205

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000020.11 Reference GRCh38.p13 Primary Assembly

    Range
    45841721..45857405 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_005260239.3XP_005260296.1  acyl-coenzyme A thioesterase 8 isoform X1

    See identical proteins and their annotated locations for XP_005260296.1

    Conserved Domains (3) summary
    cd03444
    Location:152255
    Thioesterase_II_repeat1; Thioesterase II (TEII) is thought to regenerate misprimed nonribosomal peptide synthetases (NRPSs) as well as modular polyketide synthases (PKSs) by hydrolyzing acetyl groups bound to the peptidyl carrier protein (PCP) and acyl carrier protein (ACP) ...
    TIGR00189
    Location:32256
    tesB; acyl-CoA thioesterase II
    cl00509
    Location:3577
    hot_dog; The hotdog fold was initially identified in the E. coli FabA (beta-hydroxydecanoyl-acyl carrier protein (ACP)-dehydratase) structure and subsequently in 4HBT (4-hydroxybenzoyl-CoA thioesterase) from Pseudomonas. A number of other seemingly unrelated ...
  2. XM_011528480.2XP_011526782.1  acyl-coenzyme A thioesterase 8 isoform X2

    Related
    ENSP00000498636.1, ENST00000652771.1
    Conserved Domains (1) summary
    cd03444
    Location:70173
    Thioesterase_II_repeat1; Thioesterase II (TEII) is thought to regenerate misprimed nonribosomal peptide synthetases (NRPSs) as well as modular polyketide synthases (PKSs) by hydrolyzing acetyl groups bound to the peptidyl carrier protein (PCP) and acyl carrier protein (ACP) ...

RNA

  1. XR_001754105.1 RNA Sequence

    Related
    ENST00000488679.5
  2. XR_244130.4 RNA Sequence

    Related
    ENST00000461272.5

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_183385.1: Suppressed sequence

    Description
    NM_183385.1: This RefSeq was temporary suppressed because insufficient evidence exists for the exon combination of this transcript variant, and because it is nonsense-mediated decay (NMD) candidate.
  2. NM_183386.1: Suppressed sequence

    Description
    NM_183386.1: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript and the protein.
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