U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

mthfd2 methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase [ Danio rerio (zebrafish) ]

Gene ID: 100004977, updated on 19-May-2025
Official Symbol
mthfd2provided by ZNC
Official Full Name
methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolaseprovided by ZNC
Primary source
ZFIN:ZDB-GENE-040704-20
See related
Ensembl:ENSDARG00000098646 AllianceGenome:ZFIN:ZDB-GENE-040704-20
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Danio rerio
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as
zgc:91891; wu:fb38b11; wu:fe12e11
Summary
Predicted to enable methenyltetrahydrofolate cyclohydrolase activity; methylenetetrahydrofolate dehydrogenase (NAD+) activity; and methylenetetrahydrofolate dehydrogenase (NADP+) activity. Predicted to be involved in tetrahydrofolate interconversion. Predicted to act upstream of or within one-carbon metabolic process. Predicted to be active in mitochondrion. Is expressed in anterior neural tube and optic cup. Orthologous to human MTHFD2 (methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase). [provided by Alliance of Genome Resources, May 2025]
Orthologs
human mouse all
NEW
Try the new Gene table
Try the new Transcript table
See mthfd2 in Genome Data Viewer
Location:
chromosome: 5
Exon count:
8
Annotation release Status Assembly Chr Location
RS_2025_04 current GRCz12tu (GCF_049306965.1) 5 NC_133180.1 (75731431..75748606)
RS_2024_08 previous assembly GRCz11 (GCF_000002035.6) 5 NC_007116.7 (69716508..69732746)

Chromosome 5 - NC_133180.1Genomic Context describing neighboring genes Neighboring gene MOB kinase activator 1A Neighboring gene uncharacterized LOC141385685 Neighboring gene deoxyguanosine kinase Neighboring gene ADP-ribosylation factor-like 6 interacting protein 4 Neighboring gene uncharacterized LOC101886578

Go to nucleotide: Graphics FASTA GenBank

  • Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
  • Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
  • BioProject: PRJEB1986
  • Analysis date: Fri Dec 8 19:48:10 2017

Related articles in PubMed

  1. Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells. Hitzel J, et al. Nat Commun, 2018 Jun 12. PMID 29895827, Free PMC Article
  2. XBP-1, a key regulator of unfolded protein response, activates transcription of IGF1 and Akt phosphorylation in zebrafish embryonic cell line. Hu MC, et al. Biochem Biophys Res Commun, 2007 Aug 3. PMID 17560942
  3. The N-glycome regulates the endothelial-to-hematopoietic transition. Kasper DM, et al. Science, 2020 Dec 4. PMID 33273096, Free PMC Article
  4. Disruption of the folate pathway in zebrafish causes developmental defects. Lee MS, et al. BMC Dev Biol, 2012 Apr 5. PMID 22480165, Free PMC Article
  5. Regulation of E2F1 by the von Hippel-Lindau tumour suppressor protein predicts survival in renal cell cancer patients. Mans DA, et al. J Pathol, 2013 Sep. PMID 23744542

See all (11) citations in PubMed

GeneRIFs: Gene References Into Functions

Markers

Homology

Clone Names

  • MGC91891, MGC174756

Gene Ontology Provided by ZFIN

Function Evidence Code Pubs
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables methenyltetrahydrofolate cyclohydrolase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables methenyltetrahydrofolate cyclohydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables methylenetetrahydrofolate dehydrogenase (NAD+) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables methylenetetrahydrofolate dehydrogenase (NADP+) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables methylenetetrahydrofolate dehydrogenase (NADP+) activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within one-carbon metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in tetrahydrofolate interconversion IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
is_active_in mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
  
Preferred Names
bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
NP_001002181.1

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001002181.1 → NP_001002181.1  bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial

    See identical proteins and their annotated locations for NP_001002181.1

    Status: PROVISIONAL

    Source sequence(s)
    BC074043
    UniProtKB/TrEMBL
    A0A0R4IG77, A0A2R8QHJ1, Q6GMK2
    Related
    ENSDARP00000132963.1, ENSDART00000159594.2
    Conserved Domains (3) summary
    cd01080
    Location:143 → 322
    NAD_bind_m-THF_DH_Cyclohyd; NADP binding domain of methylene-tetrahydrofolate dehydrogenase/cyclohydrolase
    COG0190
    Location:32 → 323
    FolD; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase [Coenzyme transport and metabolism]
    pfam00763
    Location:32 → 148
    THF_DHG_CYH; Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain

RefSeqs of Annotated Genomes: GCF_049306965.1-RS_2025_04

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCz12tu Primary Assembly

Genomic

  1. NC_133180.1 Reference GRCz12tu Primary Assembly

    Range
    75731431..75748606
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Molecular Biology Databases
Research Materials
Tools
Miscellaneous