GEO DataSets

(Submitter supplied) We investigate tissue transcriptomic evolution across bilaterian animals by analyzing RNA-seq data from eight different tissues across twenty species.

Organism:

Branchiostoma lanceolatum; Danio rerio; Blattella germanica; Strongylocentrotus purpuratus; Octopus bimaculoides; Strigamia maritima; Bombyx mori; Drosophila melanogaster; Callorhinchus milii; Monodelphis domestica; Episyrphus balteatus; Tribolium castaneum; Aedes aegypti; Xenopus tropicalis; Cloeon dipterum

Type:

Expression profiling by high throughput sequencing

15 related Platforms

89 Samples

Download data: TSV

(Submitter supplied) Purpose: Next-generation transcriptome sequencing was done to understand host and eukaryotic microbiome changes in gene expression in association with defined insecticide selection pressures. The specific goal of this research was to understand whole-body physiological responses in German cockroaches and associated microbiota, at the metatranscriptome level, to defined insecticide selection pressures. more...

Organism:

Blattella germanica

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30962

6 Samples

Download data: FASTA, XLSX

(Submitter supplied) Background: Germ-free or axenic organisms are valuable tools for studying immunity, digestion, and development in different hosts. Although most of these studies have been conducted on mice, recently, germ-free invertebrate models (e.g. Drosophila and Apis) are used due to their easy husbandry, low cost for production, maintenance and the high number of individuals per generation they produce. However, a limitation of using these insects is the simple bacterial community present in their guts. more...

Organism:

Periplaneta americana

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29276

18 Samples

Download data: FASTA, TAB, TSV, TXT

(Submitter supplied) Despite considerable speculation for the role of cytosine (DNA) methylation in biological and molecular processes in insects, direct functional tests are lacking. Here we provide evidence for the functional role of the maintenance DNA methyltransferase 1 (Dnmt1) in an insect using experimental manipulation. Through RNA interference (RNAi) we successfully post-transcriptionally knocked down Dnmt1 in ovarian tissue of the hemipteran Oncopeltus fasciatus (the large milkweed bug). more...

Organism:

Oncopeltus fasciatus; Blattella germanica

Type:

Methylation profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:

GPL22038 GPL24515

75 Samples

Download data: TSV, TXT, XLSX

(Submitter supplied) The success of neopteran insects, with 1 million species described, is associated with developmental innovations like the holometaboly and the evolution from short to long germ-band embryogenesis. To unveil the mechanisms underlining these innovations, we compared gene expression during the ontogeny of two extreme neopterans, the cockroach Blattella germanica (polyneopteran, hemimetabolan and short germ-band species), and the fly Drosophila melanogaster (endopterygote, holometabolan and long germ-band species). more...

Organism:

Blattella germanica

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22010

22 Samples

Download data: CSV

(Submitter supplied) Do miRNAs contribute to specify the germ-band type and the body structure in the insect embryo? Our goal was to address that issue by studying the changes in miRNA expression along the ontogeny of the German cockroach Blattella germanica, which is a short germ-band and hemimetabolan species. We sequenced small RNA libraries representing 11 developmental stages of B. germanica ontogeny (with especial emphasis on embryogenesis) and the changes in miRNA expression were examined. more...

Organism:

Blattella germanica

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL22038

22 Samples

Download data: CSV

(Submitter supplied) DNA methylation is an important chromatin modification that is necessary for the structural integrity and proper regulation of the genome for many species. Despite its conservation across the tree of life, little is known about its contribution to complex traits. Reports that differences in DNA methylation between castes in closely related Hymenopteran insects (ants, bees and wasps) contributes to social behaviors has generated hypotheses on the role of DNA methylation in governing social behavior. more...

Organism:

Blattella germanica; Periplaneta americana; Helicoverpa zea; Aedes albopictus; Aedes atropalpus; Rhyparobia maderae; Cryptocercus garciai; Aphis craccivora; Acyrthosiphon pisum; Anastrepha suspensa; Aphidius ervi; Toxorhynchites amboinensis; Pemphigus populicaulis; Pyractomena borealis; Heliothis virescens; Copidosoma floridanum; Eublaberus posticus; Reticulitermes flavipes; Microplitis demolitor; Polistes carolina; Blattella asahinai; Aedes aegypti; Anopheles gambiae; Culex quinquefasciatus; Reticulitermes virginicus; Pemphigus obesinymphae; Blaptica dubia; Pemphigus populitransversus; Microplitis mediator; Chrysodeixis includens

Type:

Methylation profiling by high throughput sequencing

30 related Platforms

69 Samples

Download data: TXT

(Submitter supplied) Is there a correlation between miRNA diversity and levels of organismic complexity? Exhibiting extraordinary levels of morphological and developmental complexity, insects are the most diverse animal class on earth. Their evolutionary success was in particular shaped by the innovation of holometabolan metamorphosis in endopterygotes. Previously, miRNA evolution had been linked to morphological complexity, but astonishing variation in the currently available miRNA complements of insects made this link unclear. more...

Organism:

Blattella germanica

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL22010

4 Samples

Download data: CSV

(Submitter supplied) The tergal gland is a structure exclusive of adult male cockroaches that produces substances attractive to the female and facilitates mating. It is formed de novo in tergites 7 and 8 during the transition from the last nymphal instar to the adult. Thus, the tergal gland can afford a suitable case study to investigate the molecular basis of a morphogenetic process occurring during metamorphosis. Using Blattella germanica as model, we constructed transcriptomes from male tergites 7-8 in non-metamorphosing specimens, and from the same tergites in metamorphosing specimens. more...

Organism:

Blattella germanica

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19525

4 Samples

Download data: TXT

(Submitter supplied) Uric acid stored in the fat body of cockroaches is a nitrogen reservoir mobilized in times of scarcity. The discovery of urease in Blattabacterium cuenoti, the primary endosymbiont of cockroaches, suggests that the endosymbiont may participate in cockroach nitrogen economy. However, bacterial urease may only be one piece in the entire nitrogen recycling process from insect uric acid. Thus, in addition to the uricolytic pathway to urea, there must be glutamine synthetase assimilating the released ammonia by the urease reaction to enable the stored nitrogen to be metabolically usable.

Organism:

Blattella germanica

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19508

3 Samples

Download data: TXT

(Submitter supplied) microRNAs (miRNAs) have been reported as key regulators in the post-transcriptional process in eukaryotic cells. In insects most of the studies have been reported in holometabolans while only recently two hemimetabolans  (Locusta migratoria and Acyrthosiphonpisum) have had their miRNAs identified. Therefore, the study on miRNAs of the evolutionarily basal hemimetabolan  Blattella germanica, may provide valuable insights on the structural and functional evolution of miRNAs. more...

Organism:

Blattella germanica

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL10675

2 Samples

Download data: TXT