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Small RNAs generated in vitro by budding-yeast Dicer

(Submitter supplied) The Dicer ribonuclease III (RNase III) enzymes process long double-stranded RNA (dsRNA) into the small interfering RNAs (siRNAs) that direct RNA interference. Here, we describe the structure and activity of a catalytically active fragment of Kluyveromyces polysporus Dcr1, which represents the noncanonical Dicers found in budding yeast. The crystal structure reveals a homodimer that resembles bacterial RNase III but includes a novel N-terminal domain and newly identified catalytic residues conserved throughout eukaryotic RNase III enzymes. more...
Organism:
Vanderwaltozyma polyspora
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL9132
2 Samples
Download data:
GEO, SRA SRP006749
Series
Accession:
GSE29168
ID:
200029168

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